Restriction map for
pFN21AE5705
##################################
# NO CUT ENZYMES ( 101 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AviII AvrII
BbrPI BciVI BfrI BglI BlnI
BlpI Bpu1102I BsaAI BsaWI BseAI
BsiEI BsiWI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII DrdI DsaI EagI
EclXI Eco47III Eco52I Eco81I EcoRV
FseI FspI HindIII HpaI KpnI
KspI MfeI MluI MroI MunI
NcoI NheI NotI NruI NspV
PacI PflMI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI ScaI SexAI
SfiI SfuI SgrAI SnaBI SpeI
SspBI SspI SwaI Van91I XbaI
XmnI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AhdI 465 GACAGACCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 465 GACAGACCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1212 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 395 TGGCCA TGG^CCA
BbeI 1171 GGCGCC GGCGC^C
BbvCI 1096 GCTGAGG CCTCAGC(-5/-2)
BcgI 168 CGATGGCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 365 TGATCA T^GATCA
BglII 1076 AGATCT A^GATCT
BsaBI 106 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 898 GAGACC GGTCTC(1/5)
BsgI 1364 GTGCAG GTGCAG(16/14)
BsmI 1324 GAATGC GAATGC(1/-1)
BsrFI 415 GCCGGC [GA]^CCGG[CT]
BstXI 248 CCAAGCAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 415 GCCGGC [GA]^CCGG[CT]
Eam1105I 465 GACAGACCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1221 CCTGGATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 1291 ATGCAT ATGCA^T
FbaI 365 TGATCA T^GATCA
HincII 451 GTTGAC GT[CT]^[GA]AC
HindII 451 GTTGAC GT[CT]^[GA]AC
KasI 1171 GGCGCC G^GCGCC
MaeII 578 ACGT A^CGT
MamI 106 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 395 TGGCCA TGG^CCA
MscI 395 TGGCCA TGG^CCA
NaeI 415 GCCGGC GCC^GGC
NarI 1171 GGCGCC GG^CGCC
NdeI 1334 CATATG CA^TATG
NgoMIV 415 GCCGGC G^CCGGC
NsiI 1291 ATGCAT ATGCA^T
NspI 204 GCATGC [GA]CATG^[CT]
PaeR7I 1825 CTCGAG C^TCGAG
PflFI 1212 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 760 GACTTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1773 CTGCAG CTGCA^G
SacI 820 GAGCTC GAGCT^C
SbfI 1772 CCTGCAGG CCTGCA^GG
SfoI 1171 GGCGCC GGC^GCC
SmaI 1570 CCCGGG CCC^GGG
SphI 204 GCATGC GCATG^C
Sse8387I 1772 CCTGCAGG CCTGCA^GG
Tth111I 1212 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 344 CCAGGGTTGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1825 CTCGAG C^TCGAG
XmaCI 1570 CCCGGG C^CCGGG
XmaI 1570 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccII 1188 CGCG CG^CG
1283 CGCG
AcsI 966 GAATTC [GA]^AATT[CT]
1598 GAATTC
AcyI 727 GACGCC G[GA]^CG[CT]C
1171 GGCGCC
ApoI 966 GAATTC [GA]^AATT[CT]
1598 GAATTC
BamHI 340 GGATCC G^GATCC
785 GGATCC
BanI 1171 GGCGCC G^G[CT][GA]CC
1267 GGCACC
BbsI 310 GAAGAC GAAGAC(2/6)
1635 GAAGAC
BfaI 688 CTAG C^TAG
1441 CTAG
BpmI 238 CTCCAG CTGGAG(16/14)
1042 CTGGAG
BpuAI 310 GAAGAC GAAGAC(2/6)
1635 GAAGAC
BsaHI 727 GACGCC G[GA]^CG[CT]C
1171 GGCGCC
BseRI 496 CTCCTC GAGGAG(10/8)
1099 GAGGAG
BspMI 749 ACCTGC ACCTGC(4/8)
1771 ACCTGC
BstEII 715 GGTGACC G^GT[ACGT]ACC
1393 GGTCACC
BstPI 715 GGTGACC G^GT[ACGT]ACC
1393 GGTCACC
BstUI 1188 CGCG CG^CG
1283 CGCG
EaeI 395 TGGCCA [CT]^GGCC[GA]
417 CGGCCA
EarI 703 CTCTTC CTCTTC(1/4)
199 GAAGAG
EcoO65I 715 GGTGACC G^GT[ACGT]ACC
1393 GGTCACC
EcoRI 966 GAATTC G^AATTC
1598 GAATTC
Hin1I 727 GACGCC G[GA]^CG[CT]C
1171 GGCGCC
Ksp632I 703 CTCTTC CTCTTC(1/4)
199 GAAGAG
MaeI 688 CTAG C^TAG
1441 CTAG
MvnI 1188 CGCG CG^CG
1283 CGCG
SapI 199 GAAGAGC GCTCTTC(1/4)
702 GCTCTTC
StuI 83 AGGCCT AGG^CCT
1437 AGGCCT
TaqI 167 TCGA T^CGA
1826 TCGA
TthHB8I 167 TCGA T^CGA
1826 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaI AvaII BanII BbvI
BcnI BmyI BsaJI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XhoII