(Circular) (Six-base) MAPSORT of: pFN21AE5705.seq Check: 566 from: 1 to: 6574 pFN21AE5705 6574 bp With 182 enzymes: SgfI * December 8, 2014 13:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2763 2763 Size: 6574 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4384 278 Size: 53 83 186 936 2848 2468 Fragments arranged by size: 2848 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3917 1937 Size: 1980 4594 Acc65I G'GTAC_C Cuts at: 3895 3895 Size: 6574 AclI AA'CG_TT Cuts at: 1563 4701 5074 1563 Size: 3138 373 3063 Fragments arranged by size: 3138 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2007 2315 2361 4637 6021 6289 1632 Size: 375 308 46 2276 1384 268 1917 Fragments arranged by size: 2276 1917 1384 375 308 268 46 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6377 AflIII A'CryG_T Cuts at: 1203 5473 6450 1203 Size: 4270 977 1327 Fragments arranged by size: 4270 1327 977 AgeI A'CCGG_T Cuts at: 5405 5405 Size: 6574 AhdI GACnn_n'nnGTC Cuts at: 2475 5303 2475 Size: 2828 3746 AleI CACnn'nnGTG Cuts at: 1166 3281 1166 Size: 2115 4459 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6574 AlwNI CAG_nnn'CTG Cuts at: 2271 3092 3203 4317 5889 2271 Size: 821 111 1114 1572 2956 Fragments arranged by size: 2956 1572 1114 821 111 ApaLI G'TGCA_C Cuts at: 4631 5787 4631 Size: 1156 5418 ApoI r'AATT_y Cuts at: 1493 2971 3603 3883 4109 4261 6370 1493 Size: 1478 632 280 226 152 2109 1697 Fragments arranged by size: 2109 1697 1478 632 280 226 152 AseI AT'TA_AT Cuts at: 160 1032 5128 160 Size: 872 4096 1606 Fragments arranged by size: 4096 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6574 AvaI C'yCGr_G Cuts at: 1870 1943 3250 3575 3830 3899 1870 Size: 73 1307 325 255 69 4545 Fragments arranged by size: 4545 1307 325 255 73 69 BamHI G'GATC_C Cuts at: 2345 2790 3904 4271 2345 Size: 445 1114 367 4648 Fragments arranged by size: 4648 1114 445 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3176 3272 3895 5350 618 Size: 325 217 627 1389 96 623 1455 1842 Fragments arranged by size: 1842 1455 1389 627 623 325 217 96 BanII G_rGCy'C Cuts at: 1391 2829 3004 3543 3576 3893 1391 Size: 1438 175 539 33 317 4072 Fragments arranged by size: 4072 1438 539 317 175 33 BbeI G_GCGC'C Cuts at: 3180 3180 Size: 6574 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2322 3647 928 Size: 971 423 1325 3855 Fragments arranged by size: 3855 1325 971 423 BbvCI CC'TCA_GC Cuts at: 3102 3102 Size: 6574 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2162 2196 4765 4799 1903 Size: 34 50 34 141 34 2569 34 3678 Fragments arranged by size: 3678 2569 141 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4466 5676 4466 Size: 1210 5364 BclI T'GATC_A Cuts at: 1548 2370 1548 Size: 822 5752 BfrBI ATG'CAT Cuts at: 3298 3298 Size: 6574 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5185 136 Size: 107 122 71 631 605 3513 1525 Fragments arranged by size: 3513 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3081 6569 3081 Size: 3488 3086 BlpI GC'TnA_GC Cuts at: 3991 3991 Size: 6574 Bme1580I G_kGCm'C Cuts at: 1790 2189 4635 5791 1790 Size: 399 2446 1156 2573 Fragments arranged by size: 2573 2446 1156 399 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6574 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2519 2733 4305 Size: 416 53 83 399 24 1102 214 1572 Cuts at: 4305 5263 442 Size: 958 1753 Fragments arranged by size: 1753 1572 1102 958 416 399 214 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6574 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3018 3018 Size: 6574 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2228 3068 5234 1239 Size: 989 840 2166 2579 Fragments arranged by size: 2579 2166 989 840 Bpu10I CC'TnA_GC Cuts at: 2481 2819 3102 3140 2481 Size: 338 283 38 5915 Fragments arranged by size: 5915 338 283 38 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2813 3332 4699 5564 5862 2813 Size: 519 1367 865 298 3525 Fragments arranged by size: 3525 1367 865 519 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2897 5237 882 Size: 617 1398 2340 2219 Fragments arranged by size: 2340 2219 1398 617 BsaAI yAC'GTr Cuts at: 493 1204 4365 493 Size: 711 3161 2702 Fragments arranged by size: 3161 2702 711 BsaBI GATnn'nnATC Cuts at: 2115 6568 2115 Size: 4453 2121 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2733 3177 4381 4763 Size: 53 83 186 936 1200 444 1204 382 Cuts at: 4763 5398 275 Size: 635 1451 Fragments arranged by size: 1451 1204 1200 936 635 444 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5007 5405 5679 5826 6237 5007 Size: 398 274 147 411 5344 Fragments arranged by size: 5344 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2852 2882 1178 Size: 30 165 30 1449 30 4870 Fragments arranged by size: 4870 1449 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2492 3119 1186 Size: 1306 627 4641 Fragments arranged by size: 4641 1306 627 BseYI C'CCAG_C Cuts at: 5777 6273 5777 Size: 496 6078 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3390 1163 Size: 573 1654 4347 Fragments arranged by size: 4347 1654 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4024 4785 5813 1540 Size: 275 186 2023 761 1028 2301 Fragments arranged by size: 2301 2023 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2658 2754 2829 3259 4635 4720 5791 2658 Size: 96 75 430 1376 85 1071 3441 Fragments arranged by size: 3441 1376 1071 430 96 85 75 BsmI GAATG_Cn' Cuts at: 3335 4085 4178 3335 Size: 750 93 5731 Fragments arranged by size: 5731 750 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2690 3200 3842 2690 Size: 510 642 5422 Fragments arranged by size: 5422 642 510 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2189 2658 2754 2829 3004 3259 3543 Size: 399 399 469 96 75 175 255 284 Cuts at: 3543 3576 3893 4635 4720 5791 1391 Size: 33 317 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 469 399 399 317 284 255 175 96 85 75 33 BspEI T'CCGG_A Cuts at: 6237 6237 Size: 6574 BspHI T'CATG_A Cuts at: 4463 6193 4463 Size: 1730 4844 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2763 3785 3929 844 Size: 1919 1022 144 3489 Fragments arranged by size: 3489 1919 1022 144 BsrBI CCG'CTC Cuts at: 1394 1823 4461 1394 Size: 429 2638 3507 Fragments arranged by size: 3507 2638 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5069 5243 65 Size: 1183 3821 174 1396 Fragments arranged by size: 3821 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2420 5218 5405 1700 Size: 110 610 2798 187 2869 Fragments arranged by size: 2869 2798 610 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6574 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6574 BssSI C'ACGA_G Cuts at: 4634 5646 4634 Size: 1012 5562 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6574 BstBI TT'CG_AA Cuts at: 5378 5378 Size: 6574 BstEII G'GTnAC_C Cuts at: 1184 2720 3398 1184 Size: 1536 678 4360 Fragments arranged by size: 4360 1536 678 BstXI CCAn_nnnn'nTGG Cuts at: 2260 2260 Size: 6574 BstYI r'GATC_y Cuts at: 1972 2345 2790 3081 3904 4271 4656 4673 6125 Size: 373 445 291 823 367 385 17 1452 Cuts at: 6125 6211 6223 6569 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 823 445 385 373 367 346 291 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6022 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2938 487 Size: 683 56 1712 4123 Fragments arranged by size: 4123 1712 683 56 BtsI GCAGTG_nn' Cuts at: 2601 4090 4884 4904 2601 Size: 1489 794 20 4271 Fragments arranged by size: 4271 1489 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6574 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6539 DraI TTT'AAA Cuts at: 3878 3878 Size: 6574 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5581 809 Size: 507 4265 1802 Fragments arranged by size: 4265 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2400 2422 4021 Size: 54 1425 50 275 18 570 22 1599 Cuts at: 4021 4910 6539 8 Size: 889 1629 43 Fragments arranged by size: 1629 1599 1425 889 570 275 54 50 43 22 18 EagI C'GGCC_G Cuts at: 1537 1812 4021 1537 Size: 275 2209 4090 Fragments arranged by size: 4090 2209 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2199 2714 4504 1353 Size: 846 515 1790 3423 Fragments arranged by size: 3423 1790 846 515 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5161 5535 5681 387 Size: 1064 138 3572 374 146 1280 Fragments arranged by size: 3572 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2827 2827 Size: 6574 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2007 2228 2315 2361 3068 4637 5234 Size: 393 375 221 87 46 707 1569 597 Cuts at: 5234 6021 6289 1239 Size: 787 268 1524 Fragments arranged by size: 1569 1524 787 707 597 393 375 268 221 87 46 EcoNI CCTnn'n_nnAGG Cuts at: 3230 3230 Size: 6574 EcoO109I rG'GnC_Cy Cuts at: 2833 3167 3216 3306 3503 2833 Size: 334 49 90 197 5904 Fragments arranged by size: 5904 334 197 90 49 EcoRI G'AATT_C Cuts at: 2971 3603 3883 4261 2971 Size: 632 280 378 5284 Fragments arranged by size: 5284 632 378 280 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3349 3419 3349 Size: 70 6504 FspI TGC'GCA Cuts at: 5080 5080 Size: 6574 HaeII r_GCGC'y Cuts at: 1678 2236 2488 3180 5721 1678 Size: 558 252 692 2541 2531 Fragments arranged by size: 2541 2531 692 558 252 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2461 2493 5215 5247 5289 Size: 32 135 32 889 32 2722 32 42 Cuts at: 5289 5321 1373 Size: 32 2626 Fragments arranged by size: 2722 2626 889 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2458 3918 4164 669 Size: 988 281 520 1460 246 3079 Fragments arranged by size: 3079 1460 988 520 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6574 HpaI GTT'AAC Cuts at: 4164 4164 Size: 6574 KasI G'GCGC_C Cuts at: 3176 3176 Size: 6574 KpnI G_GTAC'C Cuts at: 3899 3899 Size: 6574 MfeI C'AATT_G Cuts at: 4173 4173 Size: 6574 MluI A'CGCG_T Cuts at: 6450 6450 Size: 6574 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5688 5872 1305 Size: 4383 184 2007 Fragments arranged by size: 4383 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2402 10 Size: 54 1425 343 570 4182 Fragments arranged by size: 4182 1425 570 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2121 3281 3362 4532 4891 5050 518 Size: 648 955 1160 81 1170 359 159 2042 Fragments arranged by size: 2042 1170 1160 955 648 359 159 81 MspA1I CmG'CkG Cuts at: 1606 2041 2925 2978 3089 3990 4667 5815 6060 Size: 435 884 53 111 901 677 1148 245 Cuts at: 6060 1606 Size: 2120 Fragments arranged by size: 2120 1148 901 884 677 435 245 111 53 NaeI GCC'GGC Cuts at: 2422 2422 Size: 6574 NarI GG'CG_CC Cuts at: 3177 3177 Size: 6574 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6022 NdeI CA'TA_TG Cuts at: 387 3340 387 Size: 2953 3621 NgoMIV G'CCGG_C Cuts at: 2420 2420 Size: 6574 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6574 NotI GC'GGCC_GC Cuts at: 4021 4021 Size: 6574 NsiI A_TGCA'T Cuts at: 3300 3300 Size: 6574 NspI r_CATG'y Cuts at: 1132 1229 2213 3875 3932 5477 1132 Size: 97 984 1662 57 1545 2229 Fragments arranged by size: 2229 1662 1545 984 97 57 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6574 PciI A'CATG_T Cuts at: 5473 5473 Size: 6574 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6574 PfoI T'CCnGG_A Cuts at: 2534 2534 Size: 6574 PmeI GTTT'AAAC Cuts at: 3878 3878 Size: 6574 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4643 4675 6156 6188 4643 Size: 32 1481 32 5029 Fragments arranged by size: 5029 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3167 3216 3306 3503 3167 Size: 49 90 197 6238 Fragments arranged by size: 6238 197 90 49 PshAI GACnn'nnGTC Cuts at: 1613 2769 1613 Size: 1156 5418 PsiI TTA'TAA Cuts at: 4144 4144 Size: 6574 PstI C_TGCA'G Cuts at: 830 3782 3926 830 Size: 2952 144 3478 Fragments arranged by size: 3478 2952 144 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6574 PvuII CAG'CTG Cuts at: 2041 2978 3089 2041 Size: 937 111 5526 Fragments arranged by size: 5526 937 111 SacI G_AGCT'C Cuts at: 2829 2829 Size: 6574 SalI G'TCGA_C Cuts at: 1936 3916 1936 Size: 1980 4594 SanDI GG'GwC_CC Cuts at: 3167 3306 3167 Size: 139 6435 SapI GCTCTTCn'nnn_ Cuts at: 2199 2714 2199 Size: 515 6059 SbfI CC_TGCA'GG Cuts at: 3782 3926 3782 Size: 144 6430 ScaI AGT'ACT Cuts at: 4822 6534 4822 Size: 1712 4862 SfcI C'TryA_G Cuts at: 826 1045 2379 3312 3778 3922 5057 5738 5929 Size: 219 1334 933 466 144 1135 681 191 Cuts at: 5929 826 Size: 1471 Fragments arranged by size: 1471 1334 1135 933 681 466 219 191 144 SfoI GGC'GCC Cuts at: 3178 3178 Size: 6574 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6574 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6574 SmaI CCC'GGG Cuts at: 1872 3577 3901 1872 Size: 1705 324 4545 Fragments arranged by size: 4545 1705 324 SmlI C'TyrA_G Cuts at: 820 1017 1943 2828 3347 3830 4678 5579 5841 Size: 197 926 885 519 483 848 901 262 Cuts at: 5841 820 Size: 1553 Fragments arranged by size: 1553 926 901 885 848 519 483 262 197 SnaBI TAC'GTA Cuts at: 493 4365 493 Size: 3872 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6574 SphI G_CATG'C Cuts at: 1132 2213 3932 1132 Size: 1081 1719 3774 Fragments arranged by size: 3774 1719 1081 SspI AAT'ATT Cuts at: 5 52 4498 5 Size: 47 4446 2081 Fragments arranged by size: 4446 2081 47 StuI AGG'CCT Cuts at: 1517 2090 3444 1517 Size: 573 1354 4647 Fragments arranged by size: 4647 1354 573 StyI C'CwwG_G Cuts at: 513 1065 2607 2855 2913 3543 4013 513 Size: 552 1542 248 58 630 470 3074 Fragments arranged by size: 3074 1542 630 552 470 248 58 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2090 4601 4618 4771 4956 5401 325 Size: 1255 510 2511 17 153 185 445 1498 Fragments arranged by size: 2511 1498 1255 510 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4820 6532 96 Size: 274 80 33 51 1443 2843 1712 138 Fragments arranged by size: 2843 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2634 2666 2852 2884 6534 6566 2634 Size: 32 186 32 3650 32 2642 Fragments arranged by size: 3650 2642 186 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3220 1316 Size: 1904 4670 XbaI T'CTAG_A Cuts at: 3910 3910 Size: 6574 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2356 2356 Size: 6574 XhoI C'TCGA_G Cuts at: 1943 3830 1943 Size: 1887 4687 XmaI C'CCGG_G Cuts at: 1870 3575 3899 1870 Size: 1705 324 4545 Fragments arranged by size: 4545 1705 324 XmnI GAAnn'nnTTC Cuts at: 4703 4703 Size: 6574 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4382 276 Size: 53 83 186 936 2848 2468 Fragments arranged by size: 2848 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BsiWI BstZ17I Bsu36I DraIII EcoRV FseI FspAI NruI PacI PmlI PspOMI PspXI PsrI RsrII SacII SexAI SfiI SrfI SwaI