(Linear) (Six-base) MAPSORT of: KIEE5705.seq Check: 5924 from: 1 to: 1869 KIEE5705.orf With 182 enzymes: SgfI * December 5, 2014 13:07 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 758 1869 Size: 758 1111 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2 310 356 1869 Size: 2 308 46 1513 Fragments arranged by size: 1513 308 46 2 AhdI GACnn_n'nnGTC Cuts at: 0 470 1869 Size: 470 1399 AleI CACnn'nnGTG Cuts at: 0 1276 1869 Size: 1276 593 AlwNI CAG_nnn'CTG Cuts at: 0 266 1087 1198 1869 Size: 266 821 111 671 Fragments arranged by size: 821 671 266 111 ApoI r'AATT_y Cuts at: 0 966 1598 1869 Size: 966 632 271 Fragments arranged by size: 966 632 271 AvaI C'yCGr_G Cuts at: 0 1245 1570 1825 1869 Size: 1245 325 255 44 Fragments arranged by size: 1245 325 255 44 BamHI G'GATC_C Cuts at: 0 340 785 1869 Size: 340 445 1084 Fragments arranged by size: 1084 445 340 BanI G'GyrC_C Cuts at: 0 1171 1267 1869 Size: 1171 96 602 Fragments arranged by size: 1171 602 96 BanII G_rGCy'C Cuts at: 0 824 999 1538 1571 1869 Size: 824 175 539 33 298 Fragments arranged by size: 824 539 298 175 33 BbeI G_GCGC'C Cuts at: 0 1175 1869 Size: 1175 694 BbsI GAAGACnn'nnnn_ Cuts at: 0 317 1642 1869 Size: 317 1325 227 Fragments arranged by size: 1325 317 227 BbvCI CC'TCA_GC Cuts at: 0 1097 1869 Size: 1097 772 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 157 191 1869 Size: 157 34 1678 Fragments arranged by size: 1678 157 34 BclI T'GATC_A Cuts at: 0 365 1869 Size: 365 1504 BfrBI ATG'CAT Cuts at: 0 1293 1869 Size: 1293 576 BglII A'GATC_T Cuts at: 0 1076 1869 Size: 1076 793 Bme1580I G_kGCm'C Cuts at: 0 184 1869 Size: 184 1685 BmrI ACTGGGnnnn_n' Cuts at: 0 514 728 1869 Size: 514 214 1141 Fragments arranged by size: 1141 514 214 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1013 1869 Size: 1013 856 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 223 1063 1869 Size: 223 840 806 Fragments arranged by size: 840 806 223 Bpu10I CC'TnA_GC Cuts at: 0 476 814 1097 1135 1869 Size: 476 338 283 38 734 Fragments arranged by size: 734 476 338 283 38 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 808 1327 1869 Size: 808 519 542 Fragments arranged by size: 808 542 519 BsaI GGTCTCn'nnnn_ Cuts at: 0 892 1869 Size: 892 977 BsaBI GATnn'nnATC Cuts at: 0 110 1869 Size: 110 1759 BsaHI Gr'CG_yC Cuts at: 0 728 1172 1869 Size: 728 444 697 Fragments arranged by size: 728 697 444 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 847 877 1869 Size: 847 30 992 Fragments arranged by size: 992 847 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 487 1114 1869 Size: 487 627 755 Fragments arranged by size: 755 627 487 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1385 1869 Size: 1385 484 BsiHKAI G_wGCw'C Cuts at: 0 653 749 824 1254 1869 Size: 653 96 75 430 615 Fragments arranged by size: 653 615 430 96 75 BsmI GAATG_Cn' Cuts at: 0 1330 1869 Size: 1330 539 BsmBI CGTCTCn'nnnn_ Cuts at: 0 685 1195 1837 1869 Size: 685 510 642 32 Fragments arranged by size: 685 642 510 32 Bsp1286I G_dGCh'C Cuts at: 0 184 653 749 824 999 1254 1538 1571 Size: 184 469 96 75 175 255 284 33 Cuts at: 1571 1869 Size: 298 Fragments arranged by size: 469 298 284 255 184 175 96 75 33 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 758 1780 1869 Size: 758 1022 89 Fragments arranged by size: 1022 758 89 BsrFI r'CCGG_y Cuts at: 0 415 1869 Size: 415 1454 BstEII G'GTnAC_C Cuts at: 0 715 1393 1869 Size: 715 678 476 Fragments arranged by size: 715 678 476 BstXI CCAn_nnnn'nTGG Cuts at: 0 255 1869 Size: 255 1614 BstYI r'GATC_y Cuts at: 0 340 785 1076 1869 Size: 340 445 291 793 Fragments arranged by size: 793 445 340 291 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 933 1869 Size: 933 936 BtsI GCAGTG_nn' Cuts at: 0 596 1869 Size: 596 1273 EaeI y'GGCC_r Cuts at: 0 395 417 1869 Size: 395 22 1452 Fragments arranged by size: 1452 395 22 EarI CTCTTCn'nnn_ Cuts at: 0 194 709 1869 Size: 194 515 1160 Fragments arranged by size: 1160 515 194 EcoICRI GAG'CTC Cuts at: 0 822 1869 Size: 822 1047 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2 223 310 356 1063 1869 Size: 2 221 87 46 707 806 Fragments arranged by size: 806 707 221 87 46 2 EcoNI CCTnn'n_nnAGG Cuts at: 0 1225 1869 Size: 1225 644 EcoO109I rG'GnC_Cy Cuts at: 0 828 1162 1211 1301 1498 1869 Size: 828 334 49 90 197 371 Fragments arranged by size: 828 371 334 197 90 49 EcoRI G'AATT_C Cuts at: 0 966 1598 1869 Size: 966 632 271 Fragments arranged by size: 966 632 271 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1344 1414 1869 Size: 1344 70 455 Fragments arranged by size: 1344 455 70 HaeII r_GCGC'y Cuts at: 0 231 483 1175 1869 Size: 231 252 692 694 Fragments arranged by size: 694 692 252 231 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 456 488 1869 Size: 456 32 1381 Fragments arranged by size: 1381 456 32 HincII GTy'rAC Cuts at: 0 453 1869 Size: 453 1416 KasI G'GCGC_C Cuts at: 0 1171 1869 Size: 1171 698 MscI TGG'CCA Cuts at: 0 397 1869 Size: 397 1472 MslI CAynn'nnrTG Cuts at: 0 116 1276 1357 1869 Size: 116 1160 81 512 Fragments arranged by size: 1160 512 116 81 MspA1I CmG'CkG Cuts at: 0 36 920 973 1084 1869 Size: 36 884 53 111 785 Fragments arranged by size: 884 785 111 53 36 NaeI GCC'GGC Cuts at: 0 417 1869 Size: 417 1452 NarI GG'CG_CC Cuts at: 0 1172 1869 Size: 1172 697 NdeI CA'TA_TG Cuts at: 0 1335 1869 Size: 1335 534 NgoMIV G'CCGG_C Cuts at: 0 415 1869 Size: 415 1454 NsiI A_TGCA'T Cuts at: 0 1295 1869 Size: 1295 574 NspI r_CATG'y Cuts at: 0 208 1869 Size: 208 1661 PfoI T'CCnGG_A Cuts at: 0 529 1869 Size: 529 1340 PpuMI rG'GwC_Cy Cuts at: 0 1162 1211 1301 1498 1869 Size: 1162 49 90 197 371 Fragments arranged by size: 1162 371 197 90 49 PshAI GACnn'nnGTC Cuts at: 0 764 1869 Size: 764 1105 PstI C_TGCA'G Cuts at: 0 1777 1869 Size: 1777 92 PvuII CAG'CTG Cuts at: 0 36 973 1084 1869 Size: 36 937 111 785 Fragments arranged by size: 937 785 111 36 SacI G_AGCT'C Cuts at: 0 824 1869 Size: 824 1045 SanDI GG'GwC_CC Cuts at: 0 1162 1301 1869 Size: 1162 139 568 Fragments arranged by size: 1162 568 139 SapI GCTCTTCn'nnn_ Cuts at: 0 194 709 1869 Size: 194 515 1160 Fragments arranged by size: 1160 515 194 SbfI CC_TGCA'GG Cuts at: 0 1777 1869 Size: 1777 92 SfcI C'TryA_G Cuts at: 0 374 1307 1773 1869 Size: 374 933 466 96 Fragments arranged by size: 933 466 374 96 SfoI GGC'GCC Cuts at: 0 1173 1869 Size: 1173 696 SmaI CCC'GGG Cuts at: 0 1572 1869 Size: 1572 297 SmlI C'TyrA_G Cuts at: 0 823 1342 1825 1869 Size: 823 519 483 44 Fragments arranged by size: 823 519 483 44 SphI G_CATG'C Cuts at: 0 208 1869 Size: 208 1661 StuI AGG'CCT Cuts at: 0 85 1439 1869 Size: 85 1354 430 Fragments arranged by size: 1354 430 85 StyI C'CwwG_G Cuts at: 0 602 850 908 1538 1869 Size: 602 248 58 630 331 Fragments arranged by size: 630 602 331 248 58 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 85 1869 Size: 85 1784 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 629 661 847 879 1869 Size: 629 32 186 32 990 Fragments arranged by size: 990 629 186 32 32 Tth111I GACn'n_nGTC Cuts at: 0 1215 1869 Size: 1215 654 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 351 1869 Size: 351 1518 XhoI C'TCGA_G Cuts at: 0 1825 1869 Size: 1825 44 XmaI C'CCGG_G Cuts at: 0 1570 1869 Size: 1570 299 Enzymes that do cut: AarI AcuI AhdI AleI AlwNI ApoI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BclI BfrBI BglII Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaXI BseRI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrFI BstEII BstXI BstYI BtgZI BtsI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII KasI MscI MslI MspA1I NaeI NarI NdeI NgoMIV NsiI NspI PfoI PpuMI PshAI PstI PvuII SacI SanDI SapI SbfI SfcI SfoI SmaI SmlI SphI StuI StyI TaqII TstI Tth111I XcmI XhoI XmaI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BciVI BglI BlpI BmgBI BmtI BsaAI BsaWI BseYI BsiEI BsiWI BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstZ17I Bsu36I BtgI ClaI CspCI DraI DraIII DrdI EagI EciI EcoRV FseI FspI FspAI HindIII HpaI KpnI MfeI MluI MmeI NcoI NheI NotI NruI PacI PasI PciI PflMI PmeI PmlI PpiI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI TatI XbaI XmnI ZraI