Restriction map for
pFN21AE5619
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# NO CUT ENZYMES ( 138 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaLI AscI
Asp700I Asp718I AspEI AspI AvaI
AvrII BbeI BbrPI BbvCI BcgI
BclI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaHI BsaI BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
BsoBI Bsp1407I BspDI BspEI BspHI
BspMI BsrDI BsrFI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI HaeII
HgaI Hin1I HpaI KasI KpnI
KspI MfeI MluI MroI MunI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI Psp1406I
PstI PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SphI Sse8387I SspBI StuI
SwaI Tth111I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AlwNI 137 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 530 GGGCCC GGGCC^C
AseI 703 ATTAAT AT^TAAT
AsnI 703 ATTAAT AT^TAAT
AviII 1223 TGCGCA TGC^GCA
BamHI 1277 GGATCC G^GATCC
BanI 652 GGCACC G^G[CT][GA]CC
BciVI 223 GGATAC GTATCC(6/5)
BcnI 1274 CCCGG CC^[GC]GG
BsaBI 846 GATGGCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 445 GAGGAG GAGGAG(10/8)
BsmAI 1393 GAGAC GTCTC(1/5)
BsmI 817 GCATTC GAATGC(1/-1)
BspLU11I 1079 ACATGT A^CATGT
BsrBI 574 GAGCGG CCGCTC(-3/-3)
BssSI 599 CTCGTG CACGAG(-5/-1)
BstAPI 66 GCAAGAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cac8I 156 GCGTGC GC[ACGT]^[ACGT]GC
CfoI 1224 GCGC GCG^C
DraII 530 GGGCCCC [GA]G^G[ACGT]CC[CT]
DsaI 623 CCATGG C^C[GA][CT]GG
EcoO109I 530 GGGCCCC [GA]G^G[ACGT]CC[CT]
FspI 1223 TGCGCA TGC^GCA
HapII 1275 CCGG C^CGG
HhaI 1224 GCGC GCG^C
HinP1I 1224 GCGC G^CGC
HincII 1417 GTTGAC GT[CT]^[GA]AC
HindII 1417 GTTGAC GT[CT]^[GA]AC
HindIII 546 AAGCTT A^AGCTT
HpaII 1275 CCGG C^CGG
MamI 846 GATGGCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 334 CACCGATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 564 CAGCTG C[AC]G^C[GT]G
MspI 1275 CCGG C^CGG
NciI 1274 CCCGG CC^[GC]GG
NcoI 623 CCATGG C^CATGG
NspI 1079 ACATGT [GA]CATG^[CT]
PshBI 703 ATTAAT AT^TAAT
PvuII 564 CAGCTG CAG^CTG
SnaBI 1292 TACGTA TAC^GTA
SpeI 303 ACTAGT A^CTAGT
SspI 1030 AATATT AAT^ATT
TaqI 300 TCGA T^CGA
Tsp45I 805 GTGAC ^GT[GC]AC
TthHB8I 300 TCGA T^CGA
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# TWO CUT ENZYMES ( 25 enzymes) #
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AciI 585 CCGC CCGC(-3/-1)
576 GCGG
AcsI 129 AAATTC [GA]^AATT[CT]
955 GAATTT
AflIII 189 ACACGT A^C[GA][CT]GT
1079 ACATGT
ApoI 129 AAATTC [GA]^AATT[CT]
955 GAATTT
AspHI 173 GTGCTC G[AT]GC[AT]^C
1309 GAGCAC
AvaII 1135 GGACC G^G[AT]CC
1245 GGACC
BalI 810 TGGCCA TGG^CCA
867 TGGCCA
BanII 530 GGGCCC G[GA]GC[CT]^C
753 GAGCCC
BbsI 185 GAAGAC GAAGAC(2/6)
1375 GAAGAC
BpmI 506 CTCCAG CTGGAG(16/14)
61 CTGGAG
BpuAI 185 GAAGAC GAAGAC(2/6)
1375 GAAGAC
BsaAI 190 CACGTA [CT]AC^GT[GA]
1292 TACGTA
BsiHKAI 173 GTGCTC G[AT]GC[AT]^C
1309 GAGCAC
BsmFI 1299 GGGAC GGGAC(10/14)
1352 GGGAC
MaeII 191 ACGT A^CGT
1293 ACGT
MaeIII 619 GTAAC ^GT[ACGT]AC
805 GTGAC
MluNI 810 TGGCCA TGG^CCA
867 TGGCCA
MscI 810 TGGCCA TGG^CCA
867 TGGCCA
PflMI 870 CCAAGGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1260 CCAAGGACTGG
PleI 112 GACTC GAGTC(4/5)
297 GACTC
SfaNI 1221 GATGC GCATC(5/9)
1436 GCATC
SfcI 536 CTGTAG C^T[GA][CT]AG
950 CTGTAG
SmlI 571 CTTGAG C^T[CT][GA]AG
731 CTCAAG
TfiI 421 GATTC G^A[AT]TC
1270 GAATC
Van91I 870 CCAAGGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1260 CCAAGGACTGG
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# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
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AfaI AluI AlwI BbvI BfaI
BmyI BsaJI BsiYI BslI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cfr13I DdeI DpnII EaeI EarI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HinfI HphI ItaI Ksp632I MaeI
MboI MboII MflI MnlI MseI
MvaI MwoI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI StyI
Tru9I TseI Tsp509I TspRI XhoII