(Circular) (Six-base) MAPSORT of: pFN21AE5619.seq Check: 6778 from: 1 to: 6148 pFN21AE5619 6148 bp With 182 enzymes: SgfI * December 8, 2014 13:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3958 278 Size: 53 83 186 936 2422 2468 Fragments arranged by size: 2468 2422 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3491 1937 Size: 1554 4594 Acc65I G'GTAC_C Cuts at: 3469 3469 Size: 6148 AclI AA'CG_TT Cuts at: 1563 4275 4648 1563 Size: 2712 373 3063 Fragments arranged by size: 3063 2712 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2490 2539 4211 5595 5863 1632 Size: 858 49 1672 1384 268 1917 Fragments arranged by size: 1917 1672 1384 858 268 49 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5951 AflIII A'CryG_T Cuts at: 1203 2194 3084 5047 6024 1203 Size: 991 890 1963 977 1327 Fragments arranged by size: 1963 1327 991 977 890 AgeI A'CCGG_T Cuts at: 4979 4979 Size: 6148 AhdI GACnn_n'nnGTC Cuts at: 4877 4877 Size: 6148 AleI CACnn'nnGTG Cuts at: 1166 2343 1166 Size: 1177 4971 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3219 1184 Size: 2035 4113 AlwNI CAG_nnn'CTG Cuts at: 2147 3891 5463 2147 Size: 1744 1572 2832 Fragments arranged by size: 2832 1744 1572 ApaI G_GGCC'C Cuts at: 2539 2539 Size: 6148 ApaLI G'TGCA_C Cuts at: 4205 5361 4205 Size: 1156 4992 ApoI r'AATT_y Cuts at: 1493 2134 2960 3457 3683 3835 5944 1493 Size: 641 826 497 226 152 2109 1697 Fragments arranged by size: 2109 1697 826 641 497 226 152 AseI AT'TA_AT Cuts at: 160 1032 2709 4702 160 Size: 872 1677 1993 1606 Fragments arranged by size: 1993 1677 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6148 AvaI C'yCGr_G Cuts at: 1870 1943 3473 1870 Size: 73 1530 4545 Fragments arranged by size: 4545 1530 73 BamHI G'GATC_C Cuts at: 3282 3478 3845 3282 Size: 196 367 5585 Fragments arranged by size: 5585 367 196 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2657 3469 4924 618 Size: 325 217 627 870 812 1455 1842 Fragments arranged by size: 1842 1455 870 812 627 325 217 BanII G_rGCy'C Cuts at: 1391 2539 2762 3467 1391 Size: 1148 223 705 4072 Fragments arranged by size: 4072 1148 705 223 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2197 3387 928 Size: 971 298 1190 3689 Fragments arranged by size: 3689 1190 971 298 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4339 4373 1903 Size: 34 2402 34 3678 Fragments arranged by size: 3678 2402 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2222 4040 5250 2222 Size: 1818 1210 3120 Fragments arranged by size: 3120 1818 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6148 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4759 136 Size: 107 122 71 631 605 3087 1525 Fragments arranged by size: 3087 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6143 6143 Size: 6148 BlpI GC'TnA_GC Cuts at: 3565 3565 Size: 6148 Bme1580I G_kGCm'C Cuts at: 1790 2539 4209 5365 1790 Size: 749 1670 1156 2573 Fragments arranged by size: 2573 1670 1156 749 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6148 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3879 4837 442 Size: 416 53 83 399 24 2462 958 1753 Fragments arranged by size: 2462 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6148 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2087 2496 4808 1239 Size: 848 409 2312 2579 Fragments arranged by size: 2579 2312 848 409 Bpu10I CC'TnA_GC Cuts at: 3024 3024 Size: 6148 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2597 2721 4273 5138 5436 2597 Size: 124 1552 865 298 3309 Fragments arranged by size: 3309 1552 865 298 124 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4811 882 Size: 617 3312 2219 Fragments arranged by size: 3312 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2197 3299 3939 493 Size: 711 993 1102 640 2702 Fragments arranged by size: 2702 1102 993 711 640 BsaBI GATnn'nnATC Cuts at: 2855 6142 2855 Size: 3287 2861 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3955 4337 4972 275 Size: 53 83 186 936 2422 382 635 1451 Fragments arranged by size: 2422 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4581 4979 5253 5400 5811 4581 Size: 398 274 147 411 4918 Fragments arranged by size: 4918 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5923 Fragments arranged by size: 5923 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2465 1186 Size: 1279 4869 BseYI C'CCAG_C Cuts at: 2223 5351 5847 2223 Size: 3128 496 2524 Fragments arranged by size: 3128 2524 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5575 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3598 4359 5387 1540 Size: 275 186 1597 761 1028 2301 Fragments arranged by size: 2301 1597 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2182 3318 4209 4294 5365 2182 Size: 1136 891 85 1071 2965 Fragments arranged by size: 2965 1136 1071 891 85 BsmI GAATG_Cn' Cuts at: 2822 3659 3752 2822 Size: 837 93 5218 Fragments arranged by size: 5218 837 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2182 2539 2762 3318 3467 4209 4294 Size: 399 392 357 223 556 149 742 85 Cuts at: 4294 5365 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 556 399 392 357 223 149 85 BspEI T'CCGG_A Cuts at: 5811 5811 Size: 6148 BspHI T'CATG_A Cuts at: 4037 5767 4037 Size: 1730 4418 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3503 844 Size: 2659 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2581 4035 1394 Size: 429 758 1454 3507 Fragments arranged by size: 3507 1454 758 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4643 4817 65 Size: 1183 3395 174 1396 Fragments arranged by size: 3395 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4792 4979 1700 Size: 110 2982 187 2869 Fragments arranged by size: 2982 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6148 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6148 BssSI C'ACGA_G Cuts at: 2604 4208 5220 2604 Size: 1604 1012 3532 Fragments arranged by size: 3532 1604 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2077 794 Size: 1283 4865 BstBI TT'CG_AA Cuts at: 4952 4952 Size: 6148 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6148 BstYI r'GATC_y Cuts at: 1972 2475 3006 3282 3478 3845 4230 4247 5699 Size: 503 531 276 196 367 385 17 1452 Cuts at: 5699 5785 5797 6143 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 531 503 385 367 346 276 196 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2628 513 Size: 552 939 624 4033 Fragments arranged by size: 4033 939 624 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5409 Fragments arranged by size: 5409 683 56 BtsI GCAGTG_nn' Cuts at: 3664 4458 4478 3664 Size: 794 20 5334 Fragments arranged by size: 5334 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6148 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3038 3073 529 Size: 35 2474 35 3604 Fragments arranged by size: 3604 2474 35 35 DraI TTT'AAA Cuts at: 3452 3452 Size: 6148 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5155 809 Size: 507 3839 1802 Fragments arranged by size: 3839 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2185 2815 2872 Size: 54 1425 50 275 18 355 630 57 Cuts at: 2872 3595 4484 6113 8 Size: 723 889 1629 43 Fragments arranged by size: 1629 1425 889 723 630 355 275 57 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3595 1537 Size: 275 1783 4090 Fragments arranged by size: 4090 1783 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2268 2466 3404 4078 1353 Size: 915 198 938 674 3423 Fragments arranged by size: 3423 938 915 674 198 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4735 5109 5255 387 Size: 1064 138 3146 374 146 1280 Fragments arranged by size: 3146 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2087 2490 2496 2539 4211 4808 5595 Size: 393 455 403 6 43 1672 597 787 Cuts at: 5595 5863 1239 Size: 268 1524 Fragments arranged by size: 1672 1524 787 597 455 403 393 268 43 6 EcoO109I rG'GnC_Cy Cuts at: 2536 2536 Size: 6148 EcoRI G'AATT_C Cuts at: 3457 3835 3457 Size: 378 5770 FspI TGC'GCA Cuts at: 3230 4654 3230 Size: 1424 4724 FspAI rTGC'GCAy Cuts at: 3230 3230 Size: 6148 HaeII r_GCGC'y Cuts at: 1678 5295 1678 Size: 3617 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2370 2387 2402 2419 4789 Size: 32 135 32 798 17 15 17 2370 Cuts at: 4789 4821 4863 4895 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2370 798 135 42 32 32 32 32 17 17 15 HincII GTy'rAC Cuts at: 669 1657 1938 3424 3492 3738 669 Size: 988 281 1486 68 246 3079 Fragments arranged by size: 3079 1486 988 281 246 68 HindIII A'AGCT_T Cuts at: 748 2551 748 Size: 1803 4345 HpaI GTT'AAC Cuts at: 3738 3738 Size: 6148 KpnI G_GTAC'C Cuts at: 3473 3473 Size: 6148 MfeI C'AATT_G Cuts at: 3747 3747 Size: 6148 MluI A'CGCG_T Cuts at: 6024 6024 Size: 6148 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2355 5262 5446 1305 Size: 1050 2907 184 2007 Fragments arranged by size: 2907 2007 1050 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2817 2874 10 Size: 54 1425 343 985 57 3284 Fragments arranged by size: 3284 1425 985 343 57 54 MslI CAynn'nnrTG Cuts at: 518 1166 2343 4106 4465 4624 518 Size: 648 1177 1763 359 159 2042 Fragments arranged by size: 2042 1763 1177 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2571 3564 4241 5389 5634 1606 Size: 965 993 677 1148 245 2120 Fragments arranged by size: 2120 1148 993 965 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2628 513 Size: 552 939 624 4033 Fragments arranged by size: 4033 939 624 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6148 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6148 NotI GC'GGCC_GC Cuts at: 3595 3595 Size: 6148 NspI r_CATG'y Cuts at: 1132 1229 3088 3506 5051 1132 Size: 97 1859 418 1545 2229 Fragments arranged by size: 2229 1859 1545 418 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6148 PciI A'CATG_T Cuts at: 3084 5047 3084 Size: 1963 4185 PflMI CCAn_nnn'nTGG Cuts at: 1108 2881 3271 1108 Size: 1773 390 3985 Fragments arranged by size: 3985 1773 390 PfoI T'CCnGG_A Cuts at: 3278 3278 Size: 6148 PmeI GTTT'AAAC Cuts at: 3452 3452 Size: 6148 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4217 4249 5730 5762 4217 Size: 32 1481 32 4603 Fragments arranged by size: 4603 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6148 PsiI TTA'TAA Cuts at: 3718 3718 Size: 6148 PspOMI G'GGCC_C Cuts at: 2535 2535 Size: 6148 PstI C_TGCA'G Cuts at: 830 3500 830 Size: 2670 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6148 PvuII CAG'CTG Cuts at: 2571 2571 Size: 6148 SalI G'TCGA_C Cuts at: 1936 3490 1936 Size: 1554 4594 SbfI CC_TGCA'GG Cuts at: 3500 3500 Size: 6148 ScaI AGT'ACT Cuts at: 4396 6108 4396 Size: 1712 4436 SfcI C'TryA_G Cuts at: 826 1045 2541 2955 3496 4631 5312 5503 826 Size: 219 1496 414 541 1135 681 191 1471 Fragments arranged by size: 1496 1471 1135 681 541 414 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6148 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6148 SmaI CCC'GGG Cuts at: 1872 3475 1872 Size: 1603 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2576 2736 4252 5153 5415 820 Size: 197 926 633 160 1516 901 262 1553 Fragments arranged by size: 1553 1516 926 901 633 262 197 160 SnaBI TAC'GTA Cuts at: 493 3299 3939 493 Size: 2806 640 2702 Fragments arranged by size: 2806 2702 640 SpeI A'CTAG_T Cuts at: 152 2308 152 Size: 2156 3992 SphI G_CATG'C Cuts at: 1132 3506 1132 Size: 2374 3774 SspI AAT'ATT Cuts at: 5 52 3037 4072 5 Size: 47 2985 1035 2081 Fragments arranged by size: 2985 2081 1035 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6148 StyI C'CwwG_G Cuts at: 513 1065 2004 2414 2628 2875 3265 3587 513 Size: 552 939 410 214 247 390 322 3074 Fragments arranged by size: 3074 939 552 410 390 322 247 214 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2675 3333 4175 4192 4345 4530 4975 Size: 1255 1095 658 842 17 153 185 445 Cuts at: 4975 325 Size: 1498 Fragments arranged by size: 1498 1255 1095 842 658 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2740 4394 6106 Size: 274 80 33 51 1443 763 1654 1712 Cuts at: 6106 96 Size: 138 Fragments arranged by size: 1712 1654 1443 763 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3312 3344 6108 6140 3312 Size: 32 2764 32 3320 Fragments arranged by size: 3320 2764 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6148 XbaI T'CTAG_A Cuts at: 3484 3484 Size: 6148 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6148 XmaI C'CCGG_G Cuts at: 1870 3473 1870 Size: 1603 4545 XmnI GAAnn'nnTTC Cuts at: 4277 4277 Size: 6148 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3956 276 Size: 53 83 186 936 2422 2468 Fragments arranged by size: 2468 2422 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI