(Linear) (Six-base) MAPSORT of: KIEE5619.seq Check: 2043 from: 1 to: 1443 KIEE5619.orf With 182 enzymes: SgfI * December 5, 2014 13:03 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 485 534 1443 Size: 485 49 909 Fragments arranged by size: 909 485 49 AflIII A'CryG_T Cuts at: 0 189 1079 1443 Size: 189 890 364 Fragments arranged by size: 890 364 189 AleI CACnn'nnGTG Cuts at: 0 338 1443 Size: 338 1105 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1214 1443 Size: 1214 229 AlwNI CAG_nnn'CTG Cuts at: 0 142 1443 Size: 142 1301 ApaI G_GGCC'C Cuts at: 0 534 1443 Size: 534 909 ApoI r'AATT_y Cuts at: 0 129 955 1443 Size: 129 826 488 Fragments arranged by size: 826 488 129 AseI AT'TA_AT Cuts at: 0 704 1443 Size: 704 739 BamHI G'GATC_C Cuts at: 0 1277 1443 Size: 1277 166 BanI G'GyrC_C Cuts at: 0 652 1443 Size: 652 791 BanII G_rGCy'C Cuts at: 0 534 757 1443 Size: 534 223 686 Fragments arranged by size: 686 534 223 BbsI GAAGACnn'nnnn_ Cuts at: 0 192 1382 1443 Size: 192 1190 61 Fragments arranged by size: 1190 192 61 BciVI GTATCCnnnnn_n' Cuts at: 0 217 1443 Size: 217 1226 Bme1580I G_kGCm'C Cuts at: 0 534 1443 Size: 534 909 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 82 491 1443 Size: 82 409 952 Fragments arranged by size: 952 409 82 Bpu10I CC'TnA_GC Cuts at: 0 1019 1443 Size: 1019 424 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 592 716 1443 Size: 592 124 727 Fragments arranged by size: 727 592 124 BsaAI yAC'GTr Cuts at: 0 192 1294 1443 Size: 192 1102 149 Fragments arranged by size: 1102 192 149 BsaBI GATnn'nnATC Cuts at: 0 850 1443 Size: 850 593 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 460 1443 Size: 460 983 BseYI C'CCAG_C Cuts at: 0 218 1443 Size: 218 1225 BsiHKAI G_wGCw'C Cuts at: 0 177 1313 1443 Size: 177 1136 130 Fragments arranged by size: 1136 177 130 BsmI GAATG_Cn' Cuts at: 0 817 1443 Size: 817 626 Bsp1286I G_dGCh'C Cuts at: 0 177 534 757 1313 1443 Size: 177 357 223 556 130 Fragments arranged by size: 556 357 223 177 130 BsrBI CCG'CTC Cuts at: 0 576 1443 Size: 576 867 BssSI C'ACGA_G Cuts at: 0 599 1443 Size: 599 844 BstAPI GCAn_nnn'nTGC Cuts at: 0 72 1443 Size: 72 1371 BstYI r'GATC_y Cuts at: 0 470 1001 1277 1443 Size: 470 531 276 166 Fragments arranged by size: 531 470 276 166 BtgI C'CryG_G Cuts at: 0 623 1443 Size: 623 820 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1033 1068 1443 Size: 1033 35 375 Fragments arranged by size: 1033 375 35 EaeI y'GGCC_r Cuts at: 0 180 810 867 1443 Size: 180 630 57 576 Fragments arranged by size: 630 576 180 57 EarI CTCTTCn'nnn_ Cuts at: 0 263 461 1399 1443 Size: 263 198 938 44 Fragments arranged by size: 938 263 198 44 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 82 485 491 534 1443 Size: 82 403 6 43 909 Fragments arranged by size: 909 403 82 43 6 EcoO109I rG'GnC_Cy Cuts at: 0 531 1443 Size: 531 912 FspI TGC'GCA Cuts at: 0 1225 1443 Size: 1225 218 FspAI rTGC'GCAy Cuts at: 0 1225 1443 Size: 1225 218 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 365 382 397 414 1443 Size: 365 17 15 17 1029 Fragments arranged by size: 1029 365 17 17 15 HincII GTy'rAC Cuts at: 0 1419 1443 Size: 1419 24 HindIII A'AGCT_T Cuts at: 0 546 1443 Size: 546 897 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 350 1443 Size: 350 1093 MscI TGG'CCA Cuts at: 0 812 869 1443 Size: 812 57 574 Fragments arranged by size: 812 574 57 MslI CAynn'nnrTG Cuts at: 0 338 1443 Size: 338 1105 MspA1I CmG'CkG Cuts at: 0 566 1443 Size: 566 877 NcoI C'CATG_G Cuts at: 0 623 1443 Size: 623 820 NspI r_CATG'y Cuts at: 0 1083 1443 Size: 1083 360 PciI A'CATG_T Cuts at: 0 1079 1443 Size: 1079 364 PflMI CCAn_nnn'nTGG Cuts at: 0 876 1266 1443 Size: 876 390 177 Fragments arranged by size: 876 390 177 PfoI T'CCnGG_A Cuts at: 0 1273 1443 Size: 1273 170 PspOMI G'GGCC_C Cuts at: 0 530 1443 Size: 530 913 PvuII CAG'CTG Cuts at: 0 566 1443 Size: 566 877 SfcI C'TryA_G Cuts at: 0 536 950 1443 Size: 536 414 493 Fragments arranged by size: 536 493 414 SmlI C'TyrA_G Cuts at: 0 571 731 1443 Size: 571 160 712 Fragments arranged by size: 712 571 160 SnaBI TAC'GTA Cuts at: 0 1294 1443 Size: 1294 149 SpeI A'CTAG_T Cuts at: 0 303 1443 Size: 303 1140 SspI AAT'ATT Cuts at: 0 1032 1443 Size: 1032 411 StyI C'CwwG_G Cuts at: 0 409 623 870 1260 1443 Size: 409 214 247 390 183 Fragments arranged by size: 409 390 247 214 183 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 670 1328 1443 Size: 670 658 115 Fragments arranged by size: 670 658 115 TatI w'GTAC_w Cuts at: 0 735 1443 Size: 735 708 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1307 1339 1443 Size: 1307 32 104 Fragments arranged by size: 1307 104 32 Enzymes that do cut: AcuI AflIII AleI AlfI AlwNI ApaI ApoI AseI BamHI BanI BanII BbsI BciVI Bme1580I BpmI Bpu10I BpuEI BsaAI BsaBI BseRI BseYI BsiHKAI BsmI Bsp1286I BsrBI BssSI BstAPI BstYI BtgI CspCI EaeI EarI Eco57MI EcoO109I FspI FspAI Hin4I HincII HindIII MmeI MscI MslI MspA1I NcoI NspI PciI PflMI PfoI PspOMI PvuII SfcI SmlI SnaBI SpeI SspI StyI TaqII TatI TstI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AloI ApaLI AscI AsiSI AvaI AvrII BaeI BbeI BbvCI BcgI BclI BfrBI BglI BglII BlpI BmgBI BmrI BmtI BplI BsaI BsaHI BsaWI BsaXI BsgI BsiEI BsiWI BsmBI BspEI BspHI BspMI BsrDI BsrFI BsrGI BssHII BstBI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI ClaI DraI DraIII DrdI EagI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI HaeII HpaI KasI KpnI MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SphI SrfI StuI SwaI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI