################################## # NO CUT ENZYMES ( 201 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaII AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BcnI BfaI BfrI BglI BlnI BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmFI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstXI BstZ17I Bsu36I Cac8I CfoI Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FokI FseI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaI HpaII KasI KpnI Ksp632I KspI MaeI MaeII MamI MfeI MluI MluNI MroI MscI MslI MspI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsaI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tsp509I Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AciI 53 CCGC CCGC(-3/-1) AvaI 72 CCCGAG C^[CT]CG[GA]G BanII 176 GGGCTC G[GA]GC[CT]^C BclI 153 TGATCA T^GATCA BglII 65 AGATCT A^GATCT BlpI 178 GCTCAGC GC^T[ACGT]AGC BmyI 176 GGGCTC G[AGT]GC[ACT]^C BpmI 117 CTCCAG CTGGAG(16/14) Bpu1102I 178 GCTCAGC GC^T[ACGT]AGC BseRI 100 CTCCTC GAGGAG(10/8) BsoBI 72 CCCGAG C^[CT]CG[GA]G Bsp1286I 176 GGGCTC G[AGT]GC[ACT]^C BssKI 24 CCTGG ^CC[ACGT]GG BstNI 24 CCTGG CC^[AT]GG BstYI 65 AGATCT [GA]^GATC[CT] CelII 178 GCTCAGC GC^T[ACGT]AGC DdeI 179 CTCAG C^T[ACGT]AG DsaI 134 CCATGG C^C[GA][CT]GG EcoRII 24 CCTGG ^CC[AT]GG EcoT14I 134 CCATGG C^C[AT][AT]GG FbaI 153 TGATCA T^GATCA MboII 186 GAAGA GAAGA(8/7) MflI 65 AGATCT [GA]^GATC[CT] MseI 233 TTAA T^TAA MspA1I 181 CAGCTG C[AC]G^C[GT]G MvaI 24 CCTGG CC^[AT]GG MwoI 183 GCTGAAGAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NcoI 134 CCATGG C^CATGG PvuII 181 CAGCTG CAG^CTG ScrFI 24 CCTGG CC^[ACGT]GG SfaNI 227 GCATC GCATC(5/9) StyI 134 CCATGG C^C[AT][AT]GG TaqI 92 TCGA T^CGA Tru9I 233 TTAA T^TAA TthHB8I 92 TCGA T^CGA XhoII 65 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### BsaJI 23 CCCTGG C^C[ACGT][ACGT]GG 134 CCATGG BsiYI 18 CCTCACCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 99 CCTCCTCATGG BslI 18 CCTCACCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 99 CCTCCTCATGG BsrI 33 ACTGG ACTGG(1/-1) 119 CCAGT DpnII 66 GATC ^GATC 154 GATC HphI 14 TCACC GGTGA(8/7) 20 TCACC MaeIII 13 GTCAC ^GT[ACGT]AC 28 GTCAC MboI 66 GATC ^GATC 154 GATC NdeII 66 GATC ^GATC 154 GATC PstI 45 CTGCAG CTGCA^G 59 CTGCAG Sau3AI 66 GATC ^GATC 154 GATC SfcI 45 CTGCAG C^T[GA][CT]AG 59 CTGCAG Tsp45I 13 GTCAC ^GT[GC]AC 28 GTCAC TspRI 32 CACTG CA[GC]TG[ACGT][ACGT]^ 263 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 7 enzymes) # ######################################### AluI BbvI Fnu4HI ItaI MnlI NlaIII TseI