################################## # NO CUT ENZYMES ( 110 enzymes) # ################################## AatII Acc65I AclI AflII AgeI AhdI Alw44I ApaLI AscI AseI AsnI Asp718I AspEI AviII AvrII BamHI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BsaAI BsaBI BsaI BseRI BsiWI BsmI Bsp1407I BspDI BsrGI BssHII Bst1107I BstBI BstYI BstZ17I ClaI CpoI DraIII DsaI EagI Eam1105I EclXI Eco52I EcoNI EcoRV EcoT14I EcoT22I FbaI FseI FspI HindIII HpaI KpnI KspI MamI MfeI MflI MunI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI TthHB8I Van91I XbaI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 60 enzymes) # ################################### AccI 640 GTCTAC GT^[AC][GT]AC AccIII 1310 TCCGGA T^CCGGA AcsI 245 GAATTC [GA]^AATT[CT] AlwI 1285 GATCC GGATC(4/5) AlwNI 743 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 748 CCTGAGG CC^T[ACGT]AGG Aor51HI 71 AGCGCT AGC^GCT ApoI 245 GAATTC [GA]^AATT[CT] Asp700I 1005 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 754 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 293 CCCGAG C^[CT]CG[GA]G BlpI 1252 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1252 GCTCAGC GC^T[ACGT]AGC BsaWI 1310 TCCGGA [AT]^CCGG[AT] BseAI 1310 TCCGGA T^CCGGA BsiEI 769 CGGTCG CG[GA][CT]^CG BsmBI 814 CGTCTC CGTCTC(1/5) BsmFI 564 GTCCC GGGAC(10/14) BsoBI 293 CCCGAG C^[CT]CG[GA]G BspEI 1310 TCCGGA T^CCGGA BspHI 701 TCATGA T^CATGA BspLU11I 1142 ACATGT A^CATGT BsrDI 411 CATTGC GCAATG(2/0) BsrFI 692 ACCGGC [GA]^CCGG[CT] BssSI 849 CACGAG CACGAG(-5/-1) BstAPI 1362 GCAGACTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 186 GGTGACC G^GT[ACGT]ACC BstPI 186 GGTGACC G^GT[ACGT]ACC Bsu36I 748 CCTGAGG CC^T[ACGT]AGG CelII 1252 GCTCAGC GC^T[ACGT]AGC Cfr10I 692 ACCGGC [GA]^CCGG[CT] DpnII 1285 GATC ^GATC DraI 664 TTTAAA TTT^AAA DrdI 1081 GACCTGGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 697 CTCTTC CTCTTC(1/4) Eco47III 71 AGCGCT AGC^GCT Eco81I 748 CCTGAGG CC^T[ACGT]AGG EcoO65I 186 GGTGACC G^GT[ACGT]ACC EcoRI 245 GAATTC G^AATTC HincII 261 GTTGAC GT[CT]^[GA]AC HindII 261 GTTGAC GT[CT]^[GA]AC Ksp632I 697 CTCTTC CTCTTC(1/4) MboI 1285 GATC ^GATC MluI 811 ACGCGT A^CGCGT MroI 1310 TCCGGA T^CCGGA MseI 665 TTAA T^TAA NdeII 1285 GATC ^GATC PflFI 754 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 752 AGGACCC [GA]G^G[AT]CC[CT] PvuII 1255 CAGCTG CAG^CTG RcaI 701 TCATGA T^CATGA SacI 226 GAGCTC GAGCT^C SapI 696 GCTCTTC GCTCTTC(1/4) Sau3AI 1285 GATC ^GATC SphI 716 GCATGC GCATG^C Tru9I 665 TTAA T^TAA Tsp509I 246 AATT ^AATT TspRI 1110 CAGTG CA[GC]TG[ACGT][ACGT]^ Tth111I 754 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC XmnI 1005 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AccII 156 CGCG CG^CG 812 CGCG AcyI 77 GGCGCC G[GA]^CG[CT]C 557 GGCGCC ApaI 289 GGGCCC GGGCC^C 734 GGGCCC AspHI 226 GAGCTC G[AT]GC[AT]^C 1369 GTGCTC BalI 167 TGGCCA TGG^CCA 530 TGGCCA BbeI 77 GGCGCC GGCGC^C 557 GGCGCC BbsI 601 GAAGAC GAAGAC(2/6) 868 GAAGAC BcnI 81 CCGGG CC^[GC]GG 95 CCCGG BfaI 407 CTAG C^TAG 1069 CTAG BpuAI 601 GAAGAC GAAGAC(2/6) 868 GAAGAC BsaHI 77 GGCGCC G[GA]^CG[CT]C 557 GGCGCC BsiHKAI 226 GAGCTC G[AT]GC[AT]^C 1369 GTGCTC BsmAI 670 GAGAC GTCTC(1/5) 815 GTCTC BstUI 156 CGCG CG^CG 812 CGCG HgaI 810 GACGC GACGC(5/10) 813 GCGTC Hin1I 77 GGCGCC G[GA]^CG[CT]C 557 GGCGCC KasI 77 GGCGCC G^GCGCC 557 GGCGCC MaeI 407 CTAG C^TAG 1069 CTAG MaeII 125 ACGT A^CGT 1102 ACGT MluNI 167 TGGCCA TGG^CCA 530 TGGCCA MscI 167 TGGCCA TGG^CCA 530 TGGCCA MslI 469 CACAACTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1128 CATGAAGGTG MspA1I 46 CCGCTG C[AC]G^C[GT]G 1255 CAGCTG MvnI 156 CGCG CG^CG 812 CGCG NarI 77 GGCGCC GG^CGCC 557 GGCGCC NciI 81 CCGGG CC^[GC]GG 95 CCCGG PstI 685 CTGCAG CTGCA^G 1258 CTGCAG SfcI 685 CTGCAG C^T[GA][CT]AG 1258 CTGCAG SfoI 77 GGCGCC GGC^GCC 557 GGCGCC XcmI 485 CCATCATGGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1127 CCATGAAGGTGGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 58 enzymes) # ######################################### AciI AfaI AflIII AluI AvaII BanI BanII BbvI BmyI BpmI BsaJI BsgI BsiYI BslI Bsp1286I BspMI BsrBI BsrI BssKI BstF5I BstNI BstXI Cac8I CfoI Cfr13I DdeI DraII EaeI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeIII MboII MnlI MspI MvaI MwoI NlaIII NlaIV NspI PleI RsaI Sau96I ScrFI SfaNI TseI Tsp45I