(Linear) (Six-base) MAPSORT of: KIEE6441.seq Check: 2127 from: 1 to: 1392 KIEE6441.orf With 182 enzymes: SgfI * March 16, 2015 11:31 .. AccI GT'mk_AC Cuts at: 0 641 1392 Size: 641 751 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 256 310 1392 Size: 256 54 1082 Fragments arranged by size: 1082 256 54 AfeI AGC'GCT Cuts at: 0 73 1392 Size: 73 1319 AflIII A'CryG_T Cuts at: 0 811 1100 1142 1392 Size: 811 289 42 250 Fragments arranged by size: 811 289 250 42 AlwNI CAG_nnn'CTG Cuts at: 0 748 1392 Size: 748 644 ApaI G_GGCC'C Cuts at: 0 293 738 1392 Size: 293 445 654 Fragments arranged by size: 654 445 293 ApoI r'AATT_y Cuts at: 0 245 1392 Size: 245 1147 AvaI C'yCGr_G Cuts at: 0 293 1392 Size: 293 1099 BanI G'GyrC_C Cuts at: 0 77 370 557 1392 Size: 77 293 187 835 Fragments arranged by size: 835 293 187 77 BanII G_rGCy'C Cuts at: 0 230 293 738 1392 Size: 230 63 445 654 Fragments arranged by size: 654 445 230 63 BbeI G_GCGC'C Cuts at: 0 81 561 1392 Size: 81 480 831 Fragments arranged by size: 831 480 81 BbsI GAAGACnn'nnnn_ Cuts at: 0 608 875 1392 Size: 608 267 517 Fragments arranged by size: 608 517 267 BlpI GC'TnA_GC Cuts at: 0 1253 1392 Size: 1253 139 Bme1580I G_kGCm'C Cuts at: 0 293 738 1392 Size: 293 445 654 Fragments arranged by size: 654 445 293 BmgBI CAC'GTC Cuts at: 0 126 1103 1392 Size: 126 977 289 Fragments arranged by size: 977 289 126 BmrI ACTGGGnnnn_n' Cuts at: 0 751 1392 Size: 751 641 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 370 1063 1092 1392 Size: 370 693 29 300 Fragments arranged by size: 693 370 300 29 BsaHI Gr'CG_yC Cuts at: 0 78 558 1392 Size: 78 480 834 Fragments arranged by size: 834 480 78 BsaWI w'CCGG_w Cuts at: 0 1310 1392 Size: 1310 82 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 302 332 1090 1120 1392 Size: 302 30 758 30 272 Fragments arranged by size: 758 302 272 30 30 BseYI C'CCAG_C Cuts at: 0 57 117 708 793 1355 1392 Size: 57 60 591 85 562 37 Fragments arranged by size: 591 562 85 60 57 37 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1014 1081 1381 1392 Size: 1014 67 300 11 Fragments arranged by size: 1014 300 67 11 BsiEI CG_ry'CG Cuts at: 0 772 1392 Size: 772 620 BsiHKAI G_wGCw'C Cuts at: 0 230 1373 1392 Size: 230 1143 19 Fragments arranged by size: 1143 230 19 BsmBI CGTCTCn'nnnn_ Cuts at: 0 820 1392 Size: 820 572 Bsp1286I G_dGCh'C Cuts at: 0 230 293 738 1373 1392 Size: 230 63 445 635 19 Fragments arranged by size: 635 445 230 63 19 BspEI T'CCGG_A Cuts at: 0 1310 1392 Size: 1310 82 BspHI T'CATG_A Cuts at: 0 701 1392 Size: 701 691 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 692 1130 1328 1392 Size: 692 438 198 64 Fragments arranged by size: 692 438 198 64 BsrBI CCG'CTC Cuts at: 0 740 768 786 825 1392 Size: 740 28 18 39 567 Fragments arranged by size: 740 567 39 28 18 BsrDI GCAATG_nn' Cuts at: 0 410 1392 Size: 410 982 BsrFI r'CCGG_y Cuts at: 0 692 1392 Size: 692 700 BssSI C'ACGA_G Cuts at: 0 849 1392 Size: 849 543 BstAPI GCAn_nnn'nTGC Cuts at: 0 1368 1392 Size: 1368 24 BstEII G'GTnAC_C Cuts at: 0 186 1392 Size: 186 1206 BstXI CCAn_nnnn'nTGG Cuts at: 0 885 1116 1134 1392 Size: 885 231 18 258 Fragments arranged by size: 885 258 231 18 Bsu36I CC'TnA_GG Cuts at: 0 749 1392 Size: 749 643 DraI TTT'AAA Cuts at: 0 666 1392 Size: 666 726 DrdI GACnn_nn'nnGTC Cuts at: 0 1087 1392 Size: 1087 305 EaeI y'GGCC_r Cuts at: 0 167 530 826 1392 Size: 167 363 296 566 Fragments arranged by size: 566 363 296 167 EarI CTCTTCn'nnn_ Cuts at: 0 703 1392 Size: 703 689 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 625 1093 1392 Size: 625 468 299 Fragments arranged by size: 625 468 299 EcoICRI GAG'CTC Cuts at: 0 228 1392 Size: 228 1164 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 256 310 370 1063 1092 1392 Size: 256 54 60 693 29 300 Fragments arranged by size: 693 300 256 60 54 29 EcoO109I rG'GnC_Cy Cuts at: 0 290 734 753 967 1392 Size: 290 444 19 214 425 Fragments arranged by size: 444 425 290 214 19 EcoRI G'AATT_C Cuts at: 0 245 1392 Size: 245 1147 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 340 1392 Size: 340 1052 HaeII r_GCGC'y Cuts at: 0 31 75 81 561 1392 Size: 31 44 6 480 831 Fragments arranged by size: 831 480 44 31 6 HincII GTy'rAC Cuts at: 0 263 1392 Size: 263 1129 KasI G'GCGC_C Cuts at: 0 77 557 1392 Size: 77 480 835 Fragments arranged by size: 835 480 77 MluI A'CGCG_T Cuts at: 0 811 1392 Size: 811 581 MscI TGG'CCA Cuts at: 0 169 532 1392 Size: 169 363 860 Fragments arranged by size: 860 363 169 MslI CAynn'nnrTG Cuts at: 0 473 1132 1392 Size: 473 659 260 Fragments arranged by size: 659 473 260 MspA1I CmG'CkG Cuts at: 0 48 1257 1392 Size: 48 1209 135 Fragments arranged by size: 1209 135 48 NarI GG'CG_CC Cuts at: 0 78 558 1392 Size: 78 480 834 Fragments arranged by size: 834 480 78 NspI r_CATG'y Cuts at: 0 103 720 1146 1392 Size: 103 617 426 246 Fragments arranged by size: 617 426 246 103 PciI A'CATG_T Cuts at: 0 1142 1392 Size: 1142 250 PpuMI rG'GwC_Cy Cuts at: 0 753 1392 Size: 753 639 PspOMI G'GGCC_C Cuts at: 0 289 734 1392 Size: 289 445 658 Fragments arranged by size: 658 445 289 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 532 564 1392 Size: 532 32 828 Fragments arranged by size: 828 532 32 PstI C_TGCA'G Cuts at: 0 689 1262 1392 Size: 689 573 130 Fragments arranged by size: 689 573 130 PvuII CAG'CTG Cuts at: 0 1257 1392 Size: 1257 135 SacI G_AGCT'C Cuts at: 0 230 1392 Size: 230 1162 SapI GCTCTTCn'nnn_ Cuts at: 0 703 1392 Size: 703 689 SfcI C'TryA_G Cuts at: 0 685 1258 1392 Size: 685 573 134 Fragments arranged by size: 685 573 134 SfoI GGC'GCC Cuts at: 0 79 559 1392 Size: 79 480 833 Fragments arranged by size: 833 480 79 SphI G_CATG'C Cuts at: 0 720 1392 Size: 720 672 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1075 1347 1392 Size: 1075 272 45 Fragments arranged by size: 1075 272 45 TatI w'GTAC_w Cuts at: 0 321 335 1392 Size: 321 14 1057 Fragments arranged by size: 1057 321 14 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1090 1122 1392 Size: 1090 32 270 Fragments arranged by size: 1090 270 32 Tth111I GACn'n_nGTC Cuts at: 0 757 1392 Size: 757 635 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 492 1134 1392 Size: 492 642 258 Fragments arranged by size: 642 492 258 XmnI GAAnn'nnTTC Cuts at: 0 1009 1392 Size: 1009 383 Enzymes that do cut: AccI AcuI AfeI AflIII AlwNI ApaI ApoI AvaI BanI BanII BbeI BbsI BlpI Bme1580I BmgBI BmrI BpmI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstEII BstXI Bsu36I DraI DrdI EaeI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI HaeII HincII KasI MluI MscI MslI MspA1I NarI NspI PciI PpuMI PspOMI PsrI PstI PvuII SacI SapI SfcI SfoI SphI TaqII TatI TstI Tth111I XcmI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AflII AgeI AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BglI BglII BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BseRI BsiWI BsmI BsrGI BssHII BstBI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraIII EagI EcoNI EcoRV FseI FspI FspAI Hin4I HindIII HpaI KpnI MfeI MmeI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspXI PvuI RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI SspI StuI StyI SwaI XbaI XhoI XmaI ZraI