Restriction map for
pFN21AB6420
##################################
# NO CUT ENZYMES ( 141 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspHI
AspI AviII BalI BamHI BbeI
BbrPI BbsI BciVI BclI BfrI
BglI BglII BpuAI BsaAI BsaHI
BsaI BseAI BseRI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsmFI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrGI BssHII BssSI
Bst1107I BstAPI BstEII BstPI BstXI
BstZ17I Bsu36I CfoI ClaI CpoI
DraI DraII DraIII DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO109I EcoO65I EcoT22I FbaI
FseI FspI HaeII HhaI Hin1I
HinP1I HindIII HpaI KasI KpnI
MaeII MfeI MluI MluNI MroI
MscI MslI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SwaI TfiI Tth111I
Van91I
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AccI 56 GTAGAC GT^[AC][GT]AC
AccII 29 CGCG CG^CG
AcsI 874 GAATTC [GA]^AATT[CT]
AfaI 231 GTAC GT^AC
AlwNI 61 CAGTTACTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 874 GAATTC [GA]^AATT[CT]
AvaII 138 GGACC G^G[AT]CC
AvrII 985 CCTAGG C^CTAGG
BanI 240 GGTGCC G^G[CT][GA]CC
BbvCI 7 GCTGAGG CCTCAGC(-5/-2)
BcgI 306 GCAGCGGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 985 CCTAGG C^CTAGG
BlpI 889 GCTCAGC GC^T[ACGT]AGC
BpmI 713 CTCCAG CTGGAG(16/14)
Bpu1102I 889 GCTCAGC GC^T[ACGT]AGC
BsaBI 600 GATTGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 840 ACCGGA [AT]^CCGG[AT]
BsrFI 97 GCCGGT [GA]^CCGG[CT]
BstBI 46 TTCGAA TT^CGAA
BstUI 29 CGCG CG^CG
BstYI 647 AGATCC [GA]^GATC[CT]
CelII 889 GCTCAGC GC^T[ACGT]AGC
Cfr10I 97 GCCGGT [GA]^CCGG[CT]
Cfr13I 138 GGACC G^G[ACGT]CC
EcoNI 144 CCTTGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 874 GAATTC G^AATTC
EcoRV 604 GATATC GAT^ATC
HaeIII 710 GGCC GG^CC
HgaI 291 GCGTC GACGC(5/10)
HincII 277 GTCAAC GT[CT]^[GA]AC
HindII 277 GTCAAC GT[CT]^[GA]AC
HinfI 664 GACTC G^A[ACGT]TC
KspI 28 CCGCGG CCGC^GG
MamI 600 GATTGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 647 AGATCC [GA]^GATC[CT]
MseI 337 TTAA T^TAA
MvnI 29 CGCG CG^CG
NcoI 425 CCATGG C^CATGG
NspV 46 TTCGAA TT^CGAA
PaeR7I 314 CTCGAG C^TCGAG
PleI 664 GACTC GAGTC(4/5)
PvuII 22 CAGCTG CAG^CTG
RsaI 231 GTAC GT^AC
SacII 28 CCGCGG CCGC^GG
Sau96I 138 GGACC G^G[ACGT]CC
SfuI 46 TTCGAA TT^CGAA
SmaI 953 CCCGGG CCC^GGG
SspI 572 AATATT AAT^ATT
StuI 709 AGGCCT AGG^CCT
Tru9I 337 TTAA T^TAA
Tsp45I 803 GTGAC ^GT[GC]AC
XbaI 294 TCTAGA T^CTAGA
XcmI 416 CCAATTCCTCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 314 CTCGAG C^TCGAG
XhoII 647 AGATCC [GA]^GATC[CT]
XmaCI 953 CCCGGG C^CCGGG
XmaI 953 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 9 enzymes) #
###################################
AlwI 648 GATCC GGATC(4/5)
843 GGATC
Asp700I 120 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
720 GAAACCCTTC
BsmAI 662 GAGAC GTCTC(1/5)
440 GTCTC
BsrDI 349 CATTGC GCAATG(2/0)
520 GCAATG
DsaI 28 CCGCGG C^C[GA][CT]GG
425 CCATGG
FauI 94 CCCGC CCCGC(4/6)
309 GCGGG
SfaNI 454 GCATC GCATC(5/9)
679 GCATC
Tsp509I 418 AATT ^AATT
875 AATT
XmnI 120 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
720 GAAACCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AluI AvaI BanII BbvI
BcnI BfaI BmyI BsaJI BsiYI
BslI BsoBI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I DdeI DpnII
EarI EcoRII EcoT14I Fnu4HI FokI
HapII HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MnlI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV Sau3AI ScrFI
SmlI StyI TaqI TseI TspRI
TthHB8I