Restriction map for
pFN21AB6420
(Linear) (Six-base) MAPSORT of: KIBB6420.seq Check: 8692 from: 1 to: 1017
KIBB6420.orf
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AccI GT'mk_AC
Cuts at: 0 57 1017
Size: 57 960
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 247 574 1017
Size: 247 327 443
Fragments arranged by size:
443 327 247
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 109 141 926 958 1017
Size: 109 32 785 32 59
Fragments arranged by size:
785 109 59 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 66 1017
Size: 66 951
ApoI r'AATT_y
Cuts at: 0 874 1017
Size: 874 143
AvaI C'yCGr_G
Cuts at: 0 37 314 953 1017
Size: 37 277 639 64
Fragments arranged by size:
639 277 64 37
AvrII C'CTAG_G
Cuts at: 0 985 1017
Size: 985 32
BanI G'GyrC_C
Cuts at: 0 240 1017
Size: 240 777
BanII G_rGCy'C
Cuts at: 0 261 315 954 1017
Size: 261 54 639 63
Fragments arranged by size:
639 261 63 54
BbvCI CC'TCA_GC
Cuts at: 0 8 1017
Size: 8 1009
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 295 329 1017
Size: 295 34 688
Fragments arranged by size:
688 295 34
BlpI GC'TnA_GC
Cuts at: 0 890 1017
Size: 890 127
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 698 1017
Size: 698 319
Bpu10I CC'TnA_GC
Cuts at: 0 8 1017
Size: 8 1009
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 734 1017
Size: 166 568 283
Fragments arranged by size:
568 283 166
BsaBI GATnn'nnATC
Cuts at: 0 604 1017
Size: 604 413
BsaWI w'CCGG_w
Cuts at: 0 840 1017
Size: 840 177
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 109 139 496 526 926 956 1017
Size: 109 30 357 30 400 30 61
Fragments arranged by size:
400 357 109 61 30 30 30
BseYI C'CCAG_C
Cuts at: 0 283 1017
Size: 283 734
Bsp1286I G_dGCh'C
Cuts at: 0 261 315 954 1017
Size: 261 54 639 63
Fragments arranged by size:
639 261 63 54
BsrDI GCAATG_nn'
Cuts at: 0 348 527 1017
Size: 348 179 490
Fragments arranged by size:
490 348 179
BsrFI r'CCGG_y
Cuts at: 0 97 1017
Size: 97 920
BstBI TT'CG_AA
Cuts at: 0 47 1017
Size: 47 970
BstYI r'GATC_y
Cuts at: 0 647 1017
Size: 647 370
BtgI C'CryG_G
Cuts at: 0 28 425 1017
Size: 28 397 592
Fragments arranged by size:
592 397 28
BtsI GCAGTG_nn'
Cuts at: 0 410 1017
Size: 410 607
EarI CTCTTCn'nnn_
Cuts at: 0 200 611 672 917 1017
Size: 200 411 61 245 100
Fragments arranged by size:
411 245 200 100 61
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 247 574 698 1017
Size: 247 327 124 319
Fragments arranged by size:
327 319 247 124
EcoNI CCTnn'n_nnAGG
Cuts at: 0 148 1017
Size: 148 869
EcoRI G'AATT_C
Cuts at: 0 874 1017
Size: 874 143
EcoRV GAT'ATC
Cuts at: 0 606 1017
Size: 606 411
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 683 1017
Size: 683 334
HincII GTy'rAC
Cuts at: 0 279 1017
Size: 279 738
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 482 1017
Size: 482 535
MspA1I CmG'CkG
Cuts at: 0 24 30 309 1017
Size: 24 6 279 708
Fragments arranged by size:
708 279 24 6
NcoI C'CATG_G
Cuts at: 0 425 1017
Size: 425 592
PfoI T'CCnGG_A
Cuts at: 0 103 113 1017
Size: 103 10 904
Fragments arranged by size:
904 103 10
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 109 141 926 958 1017
Size: 109 32 785 32 59
Fragments arranged by size:
785 109 59 32 32
PsiI TTA'TAA
Cuts at: 0 328 1017
Size: 328 689
PvuII CAG'CTG
Cuts at: 0 24 1017
Size: 24 993
SacII CC_GC'GG
Cuts at: 0 31 1017
Size: 31 986
SmaI CCC'GGG
Cuts at: 0 955 1017
Size: 955 62
SmlI C'TyrA_G
Cuts at: 0 145 314 749 1017
Size: 145 169 435 268
Fragments arranged by size:
435 268 169 145
SspI AAT'ATT
Cuts at: 0 574 1017
Size: 574 443
StuI AGG'CCT
Cuts at: 0 711 1017
Size: 711 306
StyI C'CwwG_G
Cuts at: 0 425 857 985 1017
Size: 425 432 128 32
Fragments arranged by size:
432 425 128 32
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 994 1017
Size: 994 23
XbaI T'CTAG_A
Cuts at: 0 294 1017
Size: 294 723
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 423 1017
Size: 423 594
XhoI C'TCGA_G
Cuts at: 0 314 1017
Size: 314 703
XmaI C'CCGG_G
Cuts at: 0 953 1017
Size: 953 64
XmnI GAAnn'nnTTC
Cuts at: 0 124 724 1017
Size: 124 600 293
Fragments arranged by size:
600 293 124
Enzymes that do cut:
AccI AcuI AloI AlwNI ApoI AvaI AvrII BanI
BanII BbvCI BcgI BlpI BpmI Bpu10I BpuEI BsaBI
BsaWI BsaXI BseYI Bsp1286I BsrDI BsrFI BstBI BstYI
BtgI BtsI EarI Eco57MI EcoNI EcoRI EcoRV FalI
HincII MmeI MspA1I NcoI PfoI PpiI PsiI PvuII
SacII SmaI SmlI SspI StuI StyI TaqII XbaI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI ApaI ApaLI AscI AseI AsiSI
BaeI BamHI BbeI BbsI BciVI BclI BfrBI BglI
BglII Bme1580I BmgBI BmrI BmtI BplI BsaI BsaAI
BsaHI BseRI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI
BspEI BspHI BspMI BsrBI BsrGI BssHII BssSI BstAPI
BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DraIII DrdI EaeI EagI EciI EcoICRI EcoO109I FseI
FspI FspAI HaeII Hin4I HindIII HpaI KasI KpnI
MfeI MluI MscI MslI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PflMI PmeI PmlI PpuMI PshAI PspOMI PspXI PsrI
PstI PvuI RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SnaBI
SpeI SphI SrfI SwaI TatI TstI Tth111I ZraI