Restriction map for
pFN21AB6357
(Circular) (Six-base) MAPSORT of: pFN21AB6357.seq Check: 9069 from: 1 to: 5506
pFN21AB6357 5506 bp
With 182 enzymes: SgfI *
April 15, 2010 09:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3316 278
Size: 53 83 186 936 1780 2468
Fragments arranged by size:
2468 1780 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2849 1937
Size: 912 4594
Acc65I G'GTAC_C
Cuts at: 2827 2827
Size: 5506
AclI AA'CG_TT
Cuts at: 1563 3633 4006 1563
Size: 2070 373 3063
Fragments arranged by size:
3063 2070 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3569 4953 5221 1632
Size: 1937 1384 268 1917
Fragments arranged by size:
1937 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5309
AflIII A'CryG_T
Cuts at: 1203 2406 4405 5382 1203
Size: 1203 1999 977 1327
Fragments arranged by size:
1999 1327 1203 977
AgeI A'CCGG_T
Cuts at: 4337 4337
Size: 5506
AhdI GACnn_n'nnGTC
Cuts at: 4235 4235
Size: 5506
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5506
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5506
AlwNI CAG_nnn'CTG
Cuts at: 3249 4821 3249
Size: 1572 3934
ApaLI G'TGCA_C
Cuts at: 3563 4719 3563
Size: 1156 4350
ApoI r'AATT_y
Cuts at: 1493 2195 2731 2815 3041 3193 5302 1493
Size: 702 536 84 226 152 2109 1697
Fragments arranged by size:
2109 1697 702 536 226 152 84
AseI AT'TA_AT
Cuts at: 160 1032 4060 160
Size: 872 3028 1606
Fragments arranged by size:
3028 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5506
AvaI C'yCGr_G
Cuts at: 1870 1943 2355 2831 1870
Size: 73 412 476 4545
Fragments arranged by size:
4545 476 412 73
BamHI G'GATC_C
Cuts at: 2836 3203 2836
Size: 367 5139
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2827 4282 618
Size: 325 217 627 1040 1455 1842
Fragments arranged by size:
1842 1455 1040 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2491 2825 1391
Size: 1100 334 4072
Fragments arranged by size:
4072 1100 334
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4535
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3697 3731 1903
Size: 34 50 34 1676 34 3678
Fragments arranged by size:
3678 1676 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2712 3398 4608 2712
Size: 686 1210 3610
Fragments arranged by size:
3610 1210 686
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5506
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4117 136
Size: 107 122 71 631 605 2445 1525
Fragments arranged by size:
2445 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5501 5501
Size: 5506
BlpI GC'TnA_GC
Cuts at: 2923 2923
Size: 5506
Bme1580I G_kGCm'C
Cuts at: 1790 3567 4723 1790
Size: 1777 1156 2573
Fragments arranged by size:
2573 1777 1156
BmgBI CAC'GTC
Cuts at: 1318 2236 1318
Size: 918 4588
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3237 4195 442
Size: 416 53 83 399 24 1820 958 1753
Fragments arranged by size:
1820 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5506
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4166 1239
Size: 2927 2579
Bpu10I CC'TnA_GC
Cuts at: 2109 2109
Size: 5506
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2105 2165 3631 4496 4794 2105
Size: 60 1466 865 298 2817
Fragments arranged by size:
2817 1466 865 298 60
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2564 4169 882
Size: 617 1065 1605 2219
Fragments arranged by size:
2219 1605 1065 617
BsaAI yAC'GTr
Cuts at: 493 1204 3297 493
Size: 711 2093 2702
Fragments arranged by size:
2702 2093 711
BsaBI GATnn'nnATC
Cuts at: 5500 5500
Size: 5506
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3313 3695 4330 275
Size: 53 83 186 936 1780 382 635 1451
Fragments arranged by size:
1780 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3939 4337 4611 4758 5169 3939
Size: 398 274 147 411 4276
Fragments arranged by size:
4276 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5281
Fragments arranged by size:
5281 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2499 2580 1186
Size: 1313 81 4112
Fragments arranged by size:
4112 1313 81
BseYI C'CCAG_C
Cuts at: 4709 5205 4709
Size: 496 5010
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4933
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2956 3717 4745 1540
Size: 275 186 955 761 1028 2301
Fragments arranged by size:
2301 1028 955 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2491 3567 3652 4723 2491
Size: 1076 85 1071 3274
Fragments arranged by size:
3274 1076 1071 85
BsmI GAATG_Cn'
Cuts at: 2763 3017 3110 2763
Size: 254 93 5159
Fragments arranged by size:
5159 254 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2491 2825 3567 3652 4723 1391
Size: 399 701 334 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 701 399 334 85
BspEI T'CCGG_A
Cuts at: 5169 5169
Size: 5506
BspHI T'CATG_A
Cuts at: 2466 2644 3395 5125 2466
Size: 178 751 1730 2847
Fragments arranged by size:
2847 1730 751 178
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2861 844
Size: 2017 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3393 1394
Size: 429 1570 3507
Fragments arranged by size:
3507 1570 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4001 4175 65
Size: 1183 2753 174 1396
Fragments arranged by size:
2753 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4150 4337 1700
Size: 110 2340 187 2869
Fragments arranged by size:
2869 2340 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5506
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5506
BssSI C'ACGA_G
Cuts at: 3566 4578 3566
Size: 1012 4494
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5506
BstBI TT'CG_AA
Cuts at: 4310 4310
Size: 5506
BstEII G'GTnAC_C
Cuts at: 1184 2329 1184
Size: 1145 4361
BstYI r'GATC_y
Cuts at: 1972 2421 2722 2836 3203 3588 3605 5057 5143
Size: 449 301 114 367 385 17 1452 86
Cuts at: 5143 5155 5501 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 449 385 367 346 301 114
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2067 2067
Size: 5506
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4954
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4767
Fragments arranged by size:
4767 683 56
BtsI GCAGTG_nn'
Cuts at: 2700 3022 3816 3836 2700
Size: 322 794 20 4370
Fragments arranged by size:
4370 794 322 20
ClaI AT'CG_AT
Cuts at: 1554 2383 1554
Size: 829 4677
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5471
DraI TTT'AAA
Cuts at: 2403 2737 2810 2403
Size: 334 73 5099
Fragments arranged by size:
5099 334 73
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4513 809
Size: 507 3197 1802
Fragments arranged by size:
3197 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2653 2953 3842
Size: 54 1425 50 275 18 823 300 889
Cuts at: 3842 5471 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 823 300 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2953 1537
Size: 275 1141 4090
Fragments arranged by size:
4090 1141 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3436 1353
Size: 2083 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4093 4467 4613 387
Size: 1064 138 2504 374 146 1280
Fragments arranged by size:
2504 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2489 2489
Size: 5506
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3569 4166 4953 5221 1239
Size: 393 1937 597 787 268 1524
Fragments arranged by size:
1937 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2553 2553
Size: 5506
EcoRI G'AATT_C
Cuts at: 2815 3193 2815
Size: 378 5128
FspI TGC'GCA
Cuts at: 4012 4012
Size: 5506
HaeII r_GCGC'y
Cuts at: 1678 4653 1678
Size: 2975 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2116 2118 2148 2150 4147
Size: 32 135 32 544 2 30 2 1997
Cuts at: 4147 4179 4221 4253 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1997 544 135 42 32 32 32
32 30 2 2
HincII GTy'rAC
Cuts at: 669 1657 1938 2850 3096 669
Size: 988 281 912 246 3079
Fragments arranged by size:
3079 988 912 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5506
HpaI GTT'AAC
Cuts at: 3096 3096
Size: 5506
KpnI G_GTAC'C
Cuts at: 2831 2831
Size: 5506
MfeI C'AATT_G
Cuts at: 3105 3105
Size: 5506
MluI A'CGCG_T
Cuts at: 5382 5382
Size: 5506
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2729 4620 4804 1305
Size: 1424 1891 184 2007
Fragments arranged by size:
2007 1891 1424 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2655 10
Size: 54 1425 343 823 2861
Fragments arranged by size:
2861 1425 823 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2580 3464 3823 3982 518
Size: 648 1414 884 359 159 2042
Fragments arranged by size:
2042 1414 884 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2062 2922 3599 4747 4992 1606
Size: 456 860 677 1148 245 2120
Fragments arranged by size:
2120 1148 860 677 456 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4954
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5506
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5506
NotI GC'GGCC_GC
Cuts at: 2953 2953
Size: 5506
NspI r_CATG'y
Cuts at: 1132 1229 2410 2864 4409 1132
Size: 97 1181 454 1545 2229
Fragments arranged by size:
2229 1545 1181 454 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5506
PciI A'CATG_T
Cuts at: 2406 4405 2406
Size: 1999 3507
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2513 2662 2711 1108
Size: 1405 149 49 3903
Fragments arranged by size:
3903 1405 149 49
PmeI GTTT'AAAC
Cuts at: 2810 2810
Size: 5506
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3575 3607 5088 5120 3575
Size: 32 1481 32 3961
Fragments arranged by size:
3961 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5506
PsiI TTA'TAA
Cuts at: 3076 3076
Size: 5506
PstI C_TGCA'G
Cuts at: 830 2858 830
Size: 2028 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5506
SacI G_AGCT'C
Cuts at: 2491 2491
Size: 5506
SalI G'TCGA_C
Cuts at: 1936 2848 1936
Size: 912 4594
SbfI CC_TGCA'GG
Cuts at: 2858 2858
Size: 5506
ScaI AGT'ACT
Cuts at: 3754 5466 3754
Size: 1712 3794
SfcI C'TryA_G
Cuts at: 826 1045 2093 2335 2854 3989 4670 4861 826
Size: 219 1048 242 519 1135 681 191 1471
Fragments arranged by size:
1471 1135 1048 681 519 242 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5506
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5506
SmaI CCC'GGG
Cuts at: 1872 2833 1872
Size: 961 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2120 2180 2355 3610 4511 4773
Size: 197 926 177 60 175 1255 901 262
Cuts at: 4773 820
Size: 1553
Fragments arranged by size:
1553 1255 926 901 262 197 177 175
60
SnaBI TAC'GTA
Cuts at: 493 3297 493
Size: 2804 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5506
SphI G_CATG'C
Cuts at: 1132 2864 1132
Size: 1732 3774
SspI AAT'ATT
Cuts at: 5 52 3430 5
Size: 47 3378 2081
Fragments arranged by size:
3378 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5506
StyI C'CwwG_G
Cuts at: 513 1065 2945 513
Size: 552 1880 3074
Fragments arranged by size:
3074 1880 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3533 3550 3703 3888 4333 325
Size: 1255 1953 17 153 185 445 1498
Fragments arranged by size:
1953 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3752 5464 96
Size: 274 80 33 51 1443 1775 1712 138
Fragments arranged by size:
1775 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5466 5498 5466
Size: 32 5474
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5506
XbaI T'CTAG_A
Cuts at: 2842 2842
Size: 5506
XhoI C'TCGA_G
Cuts at: 1943 2355 1943
Size: 412 5094
XmaI C'CCGG_G
Cuts at: 1870 2831 1870
Size: 961 4545
XmnI GAAnn'nnTTC
Cuts at: 2791 3635 2791
Size: 844 4662
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3314 276
Size: 53 83 186 936 1780 2468
Fragments arranged by size:
2468 1780 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI