Restriction map for
pFN21AE1458
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# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII Acc65I AclI AcyI AflII
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
Asp718I AspEI AspHI AspI AviII
AvrII BalI BamHI BanI BanII
BbeI BbrPI BbvCI BcgI BciVI
BclI BfrI BlnI BlpI BmyI
Bpu1102I BsaAI BsaBI BsaHI BsiHKAI
BsiWI BsmBI BsmFI Bsp1286I Bsp1407I
BspHI BspLU11I BspMI BsrBI BsrDI
BsrGI BssHII BssSI BstAPI BstBI
BstEII BstPI BstXI Bsu36I CelII
CpoI DraI DraIII DrdI Eam1105I
Eco47III Eco81I EcoNI EcoO65I EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HgaI Hin1I HindIII HphI KasI
KpnI KspI MaeIII MamI MfeI
MluI MluNI MscI MunI NarI
NcoI NdeI NotI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SfcI SfiI SfoI SfuI
SgrAI SmlI SnaBI SpeI Sse8387I
SspBI SspI StuI StyI SwaI
Tsp45I Tth111I Van91I XbaI XhoI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccI 568 GTATAC GT^[AC][GT]AC
AccIII 33 TCCGGA T^CCGGA
AlwI 775 GATCC GGATC(4/5)
AseI 478 ATTAAT AT^TAAT
AsnI 478 ATTAAT AT^TAAT
Asp700I 792 GAAGTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 36 GGACC G^G[AT]CC
BbsI 491 GAAGAC GAAGAC(2/6)
BglI 734 GCCCTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 95 CTGGAG CTGGAG(16/14)
BpuAI 491 GAAGAC GAAGAC(2/6)
BsaI 601 GGTCTC GGTCTC(1/5)
BsaWI 33 TCCGGA [AT]^CCGG[AT]
BseAI 33 TCCGGA T^CCGGA
BsmI 700 GCATTC GAATGC(1/-1)
BspDI 280 ATCGAT AT^CGAT
BspEI 33 TCCGGA T^CCGGA
BsrFI 90 GCCGGC [GA]^CCGG[CT]
Bst1107I 568 GTATAC GTA^TAC
BstZ17I 568 GTATAC GTA^TAC
Cfr10I 90 GCCGGC [GA]^CCGG[CT]
ClaI 280 ATCGAT AT^CGAT
DraII 127 AGGCCCC [GA]G^G[ACGT]CC[CT]
DsaI 39 CCACGG C^C[GA][CT]GG
EaeI 42 CGGCCG [CT]^GGCC[GA]
EagI 42 CGGCCG C^GGCCG
EclXI 42 CGGCCG C^GGCCG
Eco52I 42 CGGCCG C^GGCCG
EcoO109I 127 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoRI 409 GAATTC G^AATTC
FauI 163 GCGGG CCCGC(4/6)
HincII 306 GTTAAC GT[CT]^[GA]AC
HindII 306 GTTAAC GT[CT]^[GA]AC
HpaI 306 GTTAAC GTT^AAC
MaeII 687 ACGT A^CGT
MroI 33 TCCGGA T^CCGGA
NaeI 90 GCCGGC GCC^GGC
NgoMIV 90 GCCGGC G^CCGGC
NheI 18 GCTAGC G^CTAGC
NlaIII 440 CATG CATG^
NlaIV 128 GGCCCC GG[ACGT]^[ACGT]CC
NruI 138 TCGCGA TCG^CGA
NspI 439 GCATGC [GA]CATG^[CT]
PshBI 478 ATTAAT AT^TAAT
SexAI 750 ACCTGGT A^CC[AT]GGT
SfaNI 190 GCATC GCATC(5/9)
SphI 439 GCATGC GCATG^C
XcmI 250 CCAGCGCCCTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 792 GAAGTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AcsI 289 GAATTT [GA]^AATT[CT]
409 GAATTC
AfaI 177 GTAC GT^AC
708 GTAC
ApoI 289 GAATTT [GA]^AATT[CT]
409 GAATTC
AvaI 180 CCCGGG C^[CT]CG[GA]G
194 CCCGGG
BfaI 19 CTAG C^TAG
574 CTAG
BglII 415 AGATCT A^GATCT
548 AGATCT
BseRI 121 GAGGAG GAGGAG(10/8)
145 GAGGAG
BsgI 220 CTGCAC GTGCAG(16/14)
103 GTGCAG
BsiEI 42 CGGCCG CG[GA][CT]^CG
203 CGGTCG
BsoBI 180 CCCGGG C^[CT]CG[GA]G
194 CCCGGG
BsrI 260 ACTGG ACTGG(1/-1)
705 CCAGT
BstF5I 191 CATCC GGATG(2/0)
675 GGATG
BstNI 473 CCTGG CC^[AT]GG
751 CCTGG
CfoI 7 GCGC GCG^C
253 GCGC
EarI 604 CTCTTC CTCTTC(1/4)
142 GAAGAG
EcoRII 473 CCTGG ^CC[AT]GG
751 CCTGG
FokI 191 CATCC GGATG(9/13)
675 GGATG
HaeII 6 GGCGCT [GA]GCGC^[CT]
252 AGCGCC
HhaI 7 GCGC GCG^C
253 GCGC
HinP1I 7 GCGC G^CGC
253 GCGC
Ksp632I 604 CTCTTC CTCTTC(1/4)
142 GAAGAG
MaeI 19 CTAG C^TAG
574 CTAG
MslI 360 CACATTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
362 CATTTGTGTG
MvaI 473 CCTGG CC^[AT]GG
751 CCTGG
PleI 135 GACTC GAGTC(4/5)
580 GACTC
PvuII 272 CAGCTG CAG^CTG
779 CAGCTG
RsaI 177 GTAC GT^AC
708 GTAC
SmaI 180 CCCGGG CCC^GGG
194 CCCGGG
TspRI 671 CAGTG CA[GC]TG[ACGT][ACGT]^
711 CACTG
XmaCI 180 CCCGGG C^CCGGG
194 CCCGGG
XmaI 180 CCCGGG C^CCGGG
194 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AccII AciI AluI BbvI BcnI
BsaJI BsiYI BslI BsmAI BssKI
BstUI BstYI Cac8I Cfr13I DdeI
DpnII Fnu4HI HaeIII HapII HinfI
HpaII ItaI MboI MboII MflI
MnlI MseI MspA1I MspI MvnI
MwoI NciI NdeII Sau3AI Sau96I
ScrFI TaqI TfiI Tru9I TseI
Tsp509I TthHB8I XhoII