Restriction map for
pFN21AE1458
(Circular) (Six-base) MAPSORT of: pFN21AE1458.seq Check: 8859 from: 1 to: 5518
pFN21AE1458 5518 bp
With 182 enzymes: SgfI *
August 16, 2010 14:46 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3328 278
Size: 53 83 186 936 1792 2468
Fragments arranged by size:
2468 1792 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2574 2861 1937
Size: 637 287 4594
Fragments arranged by size:
4594 637 287
Acc65I G'GTAC_C
Cuts at: 2839 2839
Size: 5518
AclI AA'CG_TT
Cuts at: 1563 3645 4018 1563
Size: 2082 373 3063
Fragments arranged by size:
3063 2082 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2327 2454 2816 3581 4965 5233 1632
Size: 695 127 362 765 1384 268 1917
Fragments arranged by size:
1917 1384 765 695 362 268 127
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5321
AflIII A'CryG_T
Cuts at: 1203 4417 5394 1203
Size: 3214 977 1327
Fragments arranged by size:
3214 1327 977
AgeI A'CCGG_T
Cuts at: 4349 4349
Size: 5518
AhdI GACnn_n'nnGTC
Cuts at: 4247 4247
Size: 5518
AleI CACnn'nnGTG
Cuts at: 1166 2369 1166
Size: 1203 4315
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5518
AlwNI CAG_nnn'CTG
Cuts at: 3261 4833 3261
Size: 1572 3946
ApaLI G'TGCA_C
Cuts at: 3575 4731 3575
Size: 1156 4362
ApoI r'AATT_y
Cuts at: 1493 2294 2414 2827 3053 3205 5314 1493
Size: 801 120 413 226 152 2109 1697
Fragments arranged by size:
2109 1697 801 413 226 152 120
AseI AT'TA_AT
Cuts at: 160 1032 2484 4072 160
Size: 872 1452 1588 1606
Fragments arranged by size:
1606 1588 1452 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5518
AvaI C'yCGr_G
Cuts at: 1870 1943 2185 2199 2843 1870
Size: 73 242 14 644 4545
Fragments arranged by size:
4545 644 242 73 14
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2165 2198 2165
Size: 33 5485
BamHI G'GATC_C
Cuts at: 2848 3215 2848
Size: 367 5151
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2839 4294 618
Size: 325 217 627 1052 1455 1842
Fragments arranged by size:
1842 1455 1052 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2837 1391
Size: 1446 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2503 928
Size: 971 604 3943
Fragments arranged by size:
3943 971 604
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3709 3743 1903
Size: 34 1772 34 3678
Fragments arranged by size:
3678 1772 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3410 4620 3410
Size: 1210 4308
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5518
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2745 4129
Size: 107 122 71 631 605 337 736 1384
Cuts at: 4129 136
Size: 1525
Fragments arranged by size:
1525 1384 736 631 605 337 122 107
71
BglII A'GATC_T
Cuts at: 2420 2553 5513 2420
Size: 133 2960 2425
Fragments arranged by size:
2960 2425 133
BlpI GC'TnA_GC
Cuts at: 2935 2935
Size: 5518
Bme1580I G_kGCm'C
Cuts at: 1790 3579 4735 1790
Size: 1789 1156 2573
Fragments arranged by size:
2573 1789 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5518
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2275 3249 4207
Size: 416 53 83 399 24 858 974 958
Cuts at: 4207 442
Size: 1753
Fragments arranged by size:
1753 974 958 858 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2027 1056
Size: 971 4547
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2121 4178 1239
Size: 882 2057 2579
Fragments arranged by size:
2579 2057 882
Bpu10I CC'TnA_GC
Cuts at: 2231 2231
Size: 5518
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3643 4508 4806 3643
Size: 865 298 4355
Fragments arranged by size:
4355 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2612 4181 882
Size: 617 1113 1569 2219
Fragments arranged by size:
2219 1569 1113 617
BsaAI yAC'GTr
Cuts at: 493 1204 3309 493
Size: 711 2105 2702
Fragments arranged by size:
2702 2105 711
BsaBI GATnn'nnATC
Cuts at: 5512 5512
Size: 5518
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3325 3707 4342 275
Size: 53 83 186 936 1792 382 635 1451
Fragments arranged by size:
1792 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2038 3951 4349 4623 4770 5181 2038
Size: 1913 398 274 147 411 2375
Fragments arranged by size:
2375 1913 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2294 2324 1178
Size: 30 165 30 891 30 4372
Fragments arranged by size:
4372 891 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2141 2165 1186
Size: 955 24 4539
Fragments arranged by size:
4539 955 24
BseYI C'CCAG_C
Cuts at: 2115 4721 5217 2115
Size: 2606 496 2416
Fragments arranged by size:
2606 2416 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2129 2210 1163
Size: 573 393 81 4471
Fragments arranged by size:
4471 573 393 81
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2050 2211 2968 3729 4757 1540
Size: 275 186 49 161 757 761 1028 2301
Fragments arranged by size:
2301 1028 761 757 275 186 161 49
BsiHKAI G_wGCw'C
Cuts at: 3579 3664 4735 3579
Size: 85 1071 4362
Fragments arranged by size:
4362 1071 85
BsmI GAATG_Cn'
Cuts at: 2705 3029 3122 2705
Size: 324 93 5101
Fragments arranged by size:
5101 324 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2837 3579 3664 4735 1391
Size: 399 1047 742 85 1071 2174
Fragments arranged by size:
2174 1071 1047 742 399 85
BspEI T'CCGG_A
Cuts at: 2038 5181 2038
Size: 3143 2375
BspHI T'CATG_A
Cuts at: 3407 5137 3407
Size: 1730 3788
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2873 844
Size: 2029 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3405 1394
Size: 429 1582 3507
Fragments arranged by size:
3507 1582 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4013 4187 65
Size: 1183 2765 174 1396
Fragments arranged by size:
2765 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2095 4162 4349 1700
Size: 110 285 2067 187 2869
Fragments arranged by size:
2869 2067 285 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5518
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5518
BssSI C'ACGA_G
Cuts at: 3578 4590 3578
Size: 1012 4506
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5518
BstBI TT'CG_AA
Cuts at: 4322 4322
Size: 5518
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5518
BstYI r'GATC_y
Cuts at: 1972 2420 2553 2779 2848 3215 3600 3617 5069
Size: 448 133 226 69 367 385 17 1452
Cuts at: 5069 5155 5167 5513 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 448 385 367 346 226 133
86 69 17 12
BstZ17I GTA'TAC
Cuts at: 2575 2575
Size: 5518
BtgI C'CryG_G
Cuts at: 513 1065 2004 2044 513
Size: 552 939 40 3987
Fragments arranged by size:
3987 939 552 40
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4779
Fragments arranged by size:
4779 683 56
BtsI GCAGTG_nn'
Cuts at: 3034 3828 3848 3034
Size: 794 20 4704
Fragments arranged by size:
4704 794 20
ClaI AT'CG_AT
Cuts at: 1554 2286 1554
Size: 732 4786
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5483
DraI TTT'AAA
Cuts at: 2822 2822
Size: 5518
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4525 809
Size: 507 3209 1802
Fragments arranged by size:
3209 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2047 2965 3854
Size: 54 1425 50 275 18 217 918 889
Cuts at: 3854 5483 8
Size: 1629 43
Fragments arranged by size:
1629 1425 918 889 275 217 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2047 2965 1537
Size: 275 235 918 4090
Fragments arranged by size:
4090 918 275 235
EarI CTCTTCn'nnn_
Cuts at: 1353 2142 2615 3448 1353
Size: 789 473 833 3423
Fragments arranged by size:
3423 833 789 473
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4105 4479 4625 387
Size: 1064 138 2516 374 146 1280
Fragments arranged by size:
2516 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2121 2327 2454 2816 3581 4178 4965
Size: 393 489 206 127 362 765 597 787
Cuts at: 4965 5233 1239
Size: 268 1524
Fragments arranged by size:
1524 787 765 597 489 393 362 268
206 127
EcoO109I rG'GnC_Cy
Cuts at: 2133 2133
Size: 5518
EcoRI G'AATT_C
Cuts at: 2414 2827 3205 2414
Size: 413 378 4727
Fragments arranged by size:
4727 413 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2525 2525
Size: 5518
FspI TGC'GCA
Cuts at: 4024 4024
Size: 5518
HaeII r_GCGC'y
Cuts at: 1678 2015 2261 4665 1678
Size: 337 246 2404 2531
Fragments arranged by size:
2531 2404 337 246
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4159 4191 4233 4265 1373
Size: 32 135 32 2587 32 42 32 2626
Fragments arranged by size:
2626 2587 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2313 2862 3108 669
Size: 988 281 375 549 246 3079
Fragments arranged by size:
3079 988 549 375 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5518
HpaI GTT'AAC
Cuts at: 2313 3108 2313
Size: 795 4723
KpnI G_GTAC'C
Cuts at: 2843 2843
Size: 5518
MfeI C'AATT_G
Cuts at: 3117 3117
Size: 5518
MluI A'CGCG_T
Cuts at: 5394 5394
Size: 5518
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2402 4632 4816 1305
Size: 1097 2230 184 2007
Fragments arranged by size:
2230 2007 1097 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3696
Fragments arranged by size:
3696 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2369 2371 3476 3835 3994 518
Size: 648 1203 2 1105 359 159 2042
Fragments arranged by size:
2042 1203 1105 648 359 159 2
MspA1I CmG'CkG
Cuts at: 1606 2020 2279 2786 2934 3611 4759 5004 1606
Size: 414 259 507 148 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 507 414 259 245 148
NaeI GCC'GGC
Cuts at: 2097 2097
Size: 5518
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4027
Fragments arranged by size:
4027 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5518
NgoMIV G'CCGG_C
Cuts at: 2095 2095
Size: 5518
NheI G'CTAG_C
Cuts at: 1052 2023 1052
Size: 971 4547
NotI GC'GGCC_GC
Cuts at: 2965 2965
Size: 5518
NruI TCG'CGA
Cuts at: 2145 2145
Size: 5518
NspI r_CATG'y
Cuts at: 1132 1229 2448 2876 4421 1132
Size: 97 1219 428 1545 2229
Fragments arranged by size:
2229 1545 1219 428 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5518
PciI A'CATG_T
Cuts at: 4417 4417
Size: 5518
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5518
PmeI GTTT'AAAC
Cuts at: 2822 2822
Size: 5518
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3587 3619 5100 5132 3587
Size: 32 1481 32 3973
Fragments arranged by size:
3973 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5518
PsiI TTA'TAA
Cuts at: 3088 3088
Size: 5518
PstI C_TGCA'G
Cuts at: 830 2870 830
Size: 2040 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5518
PvuII CAG'CTG
Cuts at: 2279 2786 2279
Size: 507 5011
SalI G'TCGA_C
Cuts at: 1936 2860 1936
Size: 924 4594
SbfI CC_TGCA'GG
Cuts at: 2870 2870
Size: 5518
ScaI AGT'ACT
Cuts at: 3766 5478 3766
Size: 1712 3806
SexAI A'CCwGG_T
Cuts at: 2755 2755
Size: 5518
SfcI C'TryA_G
Cuts at: 826 1045 2866 4001 4682 4873 826
Size: 219 1821 1135 681 191 1471
Fragments arranged by size:
1821 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5518
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5518
SmaI CCC'GGG
Cuts at: 1872 2187 2201 2845 1872
Size: 315 14 644 4545
Fragments arranged by size:
4545 644 315 14
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3622 4523 4785 820
Size: 197 926 1679 901 262 1553
Fragments arranged by size:
1679 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3309 493
Size: 2816 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5518
SphI G_CATG'C
Cuts at: 1132 2448 2876 1132
Size: 1316 428 3774
Fragments arranged by size:
3774 1316 428
SspI AAT'ATT
Cuts at: 5 52 3442 5
Size: 47 3390 2081
Fragments arranged by size:
3390 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5518
StyI C'CwwG_G
Cuts at: 513 1065 2004 2957 513
Size: 552 939 953 3074
Fragments arranged by size:
3074 953 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2197 3545 3562 3715 3900 4345 325
Size: 1255 617 1348 17 153 185 445 1498
Fragments arranged by size:
1498 1348 1255 617 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2712 3764 5476
Size: 274 80 33 51 1443 735 1052 1712
Cuts at: 5476 96
Size: 138
Fragments arranged by size:
1712 1443 1052 735 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2265 2297 5478 5510 2265
Size: 32 3181 32 2273
Fragments arranged by size:
3181 2273 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5518
XbaI T'CTAG_A
Cuts at: 2854 2854
Size: 5518
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2262 2262
Size: 5518
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5518
XmaI C'CCGG_G
Cuts at: 1870 2185 2199 2843 1870
Size: 315 14 644 4545
Fragments arranged by size:
4545 644 315 14
XmnI GAAnn'nnTTC
Cuts at: 2801 3647 2801
Size: 846 4672
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3326 276
Size: 53 83 186 936 1792 2468
Fragments arranged by size:
2468 1792 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NruI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI
EcoNI EcoRV FseI FspAI KasI NarI NsiI PacI
PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SapI SfiI SfoI SrfI SwaI