(Circular) (Six-base) MAPSORT of: pFN21AB4675.seq Check: 5701 from: 1 to: 6187 pFN21AB4675 6187 bp With 182 enzymes: SgfI * October 20, 2009 10:59 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2405 2405 Size: 6187 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3997 278 Size: 53 83 186 936 2461 2468 Fragments arranged by size: 2468 2461 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3530 1937 Size: 1593 4594 Acc65I G'GTAC_C Cuts at: 3508 3508 Size: 6187 AclI AA'CG_TT Cuts at: 1563 2915 4314 4687 1563 Size: 1352 1399 373 3063 Fragments arranged by size: 3063 1399 1352 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2415 2430 2805 2807 2870 3285 4250 5634 Size: 783 15 375 2 63 415 965 1384 Cuts at: 5634 5902 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 965 783 415 375 268 63 15 2 AflII C'TTAA_G Cuts at: 820 1017 3035 820 Size: 197 2018 3972 Fragments arranged by size: 3972 2018 197 AflIII A'CryG_T Cuts at: 1203 5086 6063 1203 Size: 3883 977 1327 Fragments arranged by size: 3883 1327 977 AgeI A'CCGG_T Cuts at: 5018 5018 Size: 6187 AhdI GACnn_n'nnGTC Cuts at: 4916 4916 Size: 6187 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6187 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2943 1184 Size: 1759 4428 AlwNI CAG_nnn'CTG Cuts at: 2783 3930 5502 2783 Size: 1147 1572 3468 Fragments arranged by size: 3468 1572 1147 ApaLI G'TGCA_C Cuts at: 4244 5400 4244 Size: 1156 5031 ApoI r'AATT_y Cuts at: 1493 2045 3047 3122 3496 3722 3874 5983 1493 Size: 552 1002 75 374 226 152 2109 1697 Fragments arranged by size: 2109 1697 1002 552 374 226 152 75 AseI AT'TA_AT Cuts at: 160 1032 4741 160 Size: 872 3709 1606 Fragments arranged by size: 3709 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6187 AvaI C'yCGr_G Cuts at: 1870 1943 3512 1870 Size: 73 1569 4545 Fragments arranged by size: 4545 1569 73 BamHI G'GATC_C Cuts at: 3517 3884 3517 Size: 367 5820 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2417 3508 4963 618 Size: 325 217 627 630 1091 1455 1842 Fragments arranged by size: 1842 1455 1091 630 627 325 217 BanII G_rGCy'C Cuts at: 1391 3506 1391 Size: 2115 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2257 2280 928 Size: 971 358 23 4835 Fragments arranged by size: 4835 971 358 23 BbvCI CC'TCA_GC Cuts at: 2251 2251 Size: 6187 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4378 4412 1903 Size: 34 50 34 2357 34 3678 Fragments arranged by size: 3678 2357 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2610 3245 4079 5289 2610 Size: 635 834 1210 3508 Fragments arranged by size: 3508 1210 834 635 BclI T'GATC_A Cuts at: 1548 1548 Size: 6187 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4798 136 Size: 107 122 71 631 605 3126 1525 Fragments arranged by size: 3126 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6182 6182 Size: 6187 BlpI GC'TnA_GC Cuts at: 2097 2828 3604 2097 Size: 731 776 4680 Fragments arranged by size: 4680 776 731 Bme1580I G_kGCm'C Cuts at: 1790 4248 5404 1790 Size: 2458 1156 2573 Fragments arranged by size: 2573 2458 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6187 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3918 4876 442 Size: 416 53 83 399 24 2501 958 1753 Fragments arranged by size: 2501 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6187 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2701 2701 Size: 6187 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2136 4847 1239 Size: 897 2711 2579 Fragments arranged by size: 2711 2579 897 Bpu10I CC'TnA_GC Cuts at: 2251 2251 Size: 6187 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2443 4312 5177 5475 2443 Size: 1869 865 298 3155 Fragments arranged by size: 3155 1869 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4850 882 Size: 617 3351 2219 Fragments arranged by size: 3351 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3978 493 Size: 711 2774 2702 Fragments arranged by size: 2774 2702 711 BsaBI GATnn'nnATC Cuts at: 6181 6181 Size: 6187 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3994 4376 5011 275 Size: 53 83 186 936 2461 382 635 1451 Fragments arranged by size: 2461 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4620 5018 5292 5439 5850 4620 Size: 398 274 147 411 4957 Fragments arranged by size: 4957 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5962 Fragments arranged by size: 5962 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2677 1186 Size: 1491 4696 BseYI C'CCAG_C Cuts at: 5390 5886 5390 Size: 496 5691 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5614 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3637 4398 5426 1540 Size: 275 186 1636 761 1028 2301 Fragments arranged by size: 2301 1636 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4248 4333 5404 4248 Size: 85 1071 5031 Fragments arranged by size: 5031 1071 85 BsmI GAATG_Cn' Cuts at: 2359 3028 3698 3791 2359 Size: 669 670 93 4755 Fragments arranged by size: 4755 670 669 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3506 4248 4333 5404 1391 Size: 399 1716 742 85 1071 2174 Fragments arranged by size: 2174 1716 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5850 5850 Size: 6187 BspHI T'CATG_A Cuts at: 4076 5806 4076 Size: 1730 4457 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2405 3542 844 Size: 1561 1137 3489 Fragments arranged by size: 3489 1561 1137 BsrBI CCG'CTC Cuts at: 1394 1823 2011 4074 1394 Size: 429 188 2063 3507 Fragments arranged by size: 3507 2063 429 188 BsrDI GCAATG_nn' Cuts at: 65 1248 4682 4856 65 Size: 1183 3434 174 1396 Fragments arranged by size: 3434 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2958 4831 5018 1700 Size: 110 1148 1873 187 2869 Fragments arranged by size: 2869 1873 1148 187 110 BsrGI T'GTAC_A Cuts at: 96 2774 3080 96 Size: 2678 306 3203 Fragments arranged by size: 3203 2678 306 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6187 BssSI C'ACGA_G Cuts at: 4247 5259 4247 Size: 1012 5175 BstAPI GCAn_nnn'nTGC Cuts at: 794 2539 794 Size: 1745 4442 BstBI TT'CG_AA Cuts at: 4991 4991 Size: 6187 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6187 BstXI CCAn_nnnn'nTGG Cuts at: 2986 2986 Size: 6187 BstYI r'GATC_y Cuts at: 1972 3130 3517 3884 4269 4286 5738 5824 5836 Size: 1158 387 367 385 17 1452 86 12 Cuts at: 5836 6182 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1158 387 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2490 513 Size: 552 1425 4210 Fragments arranged by size: 4210 1425 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5448 Fragments arranged by size: 5448 683 56 BtsI GCAGTG_nn' Cuts at: 3703 4497 4517 3703 Size: 794 20 5373 Fragments arranged by size: 5373 794 20 ClaI AT'CG_AT Cuts at: 1554 2733 1554 Size: 1179 5008 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3229 3264 529 Size: 35 2665 35 3452 Fragments arranged by size: 3452 2665 35 35 DraI TTT'AAA Cuts at: 3491 3491 Size: 6187 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5194 809 Size: 507 3878 1802 Fragments arranged by size: 3878 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3634 4523 6152 Size: 54 1425 50 275 18 1804 889 1629 Cuts at: 6152 8 Size: 43 Fragments arranged by size: 1804 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3634 1537 Size: 275 1822 4090 Fragments arranged by size: 4090 1822 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2241 3465 4117 1353 Size: 888 1224 652 3423 Fragments arranged by size: 3423 1224 888 652 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4774 5148 5294 387 Size: 1064 138 3185 374 146 1280 Fragments arranged by size: 3185 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2136 2415 2430 2805 2807 2870 3285 Size: 393 504 279 15 375 2 63 415 Cuts at: 3285 4250 4847 5634 5902 1239 Size: 965 597 787 268 1524 Fragments arranged by size: 1524 965 787 597 504 415 393 375 279 268 63 15 2 EcoRI G'AATT_C Cuts at: 3496 3874 3496 Size: 378 5809 EcoRV GAT'ATC Cuts at: 3391 3391 Size: 6187 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2417 2417 Size: 6187 FspI TGC'GCA Cuts at: 4693 4693 Size: 6187 HaeII r_GCGC'y Cuts at: 1678 5334 1678 Size: 3656 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2675 2707 2999 3031 3221 Size: 32 135 32 1103 32 292 32 190 Cuts at: 3221 3253 4828 4860 4902 4934 1373 Size: 32 1575 32 42 32 2626 Fragments arranged by size: 2626 1575 1103 292 190 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2112 3531 3777 669 Size: 988 281 174 1419 246 3079 Fragments arranged by size: 3079 1419 988 281 246 174 HindIII A'AGCT_T Cuts at: 748 748 Size: 6187 HpaI GTT'AAC Cuts at: 3777 3777 Size: 6187 KpnI G_GTAC'C Cuts at: 3512 3512 Size: 6187 MfeI C'AATT_G Cuts at: 3786 3786 Size: 6187 MluI A'CGCG_T Cuts at: 6063 6063 Size: 6187 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2594 2936 3042 5301 5485 1305 Size: 1289 342 106 2259 184 2007 Fragments arranged by size: 2259 2007 1289 342 184 106 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4365 Fragments arranged by size: 4365 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2984 4145 4504 4663 518 Size: 648 1818 1161 359 159 2042 Fragments arranged by size: 2042 1818 1161 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2101 3603 4280 5428 5673 1606 Size: 495 1502 677 1148 245 2120 Fragments arranged by size: 2120 1502 1148 677 495 245 NaeI GCC'GGC Cuts at: 2960 2960 Size: 6187 NcoI C'CATG_G Cuts at: 513 1065 2490 513 Size: 552 1425 4210 Fragments arranged by size: 4210 1425 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6187 NgoMIV G'CCGG_C Cuts at: 2958 2958 Size: 6187 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6187 NotI GC'GGCC_GC Cuts at: 3634 3634 Size: 6187 NspI r_CATG'y Cuts at: 1132 1229 3545 5090 1132 Size: 97 2316 1545 2229 Fragments arranged by size: 2316 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6187 PciI A'CATG_T Cuts at: 5086 5086 Size: 6187 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6187 PmeI GTTT'AAAC Cuts at: 3491 3491 Size: 6187 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4256 4288 5769 5801 4256 Size: 32 1481 32 4642 Fragments arranged by size: 4642 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2117 1613 Size: 504 5683 PsiI TTA'TAA Cuts at: 3003 3757 3003 Size: 754 5433 PstI C_TGCA'G Cuts at: 830 2467 2535 3539 830 Size: 1637 68 1004 3478 Fragments arranged by size: 3478 1637 1004 68 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6187 PvuII CAG'CTG Cuts at: 2101 2101 Size: 6187 SalI G'TCGA_C Cuts at: 1936 3529 1936 Size: 1593 4594 SapI GCTCTTCn'nnn_ Cuts at: 2241 2241 Size: 6187 SbfI CC_TGCA'GG Cuts at: 3539 3539 Size: 6187 ScaI AGT'ACT Cuts at: 3273 4435 6147 3273 Size: 1162 1712 3313 Fragments arranged by size: 3313 1712 1162 SfcI C'TryA_G Cuts at: 826 1045 2463 2531 3535 4670 5351 5542 826 Size: 219 1418 68 1004 1135 681 191 1471 Fragments arranged by size: 1471 1418 1135 1004 681 219 191 68 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6187 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6187 SmaI CCC'GGG Cuts at: 1872 3514 1872 Size: 1642 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2422 3035 4291 5192 5454 820 Size: 197 926 479 613 1256 901 262 1553 Fragments arranged by size: 1553 1256 926 901 613 479 262 197 SnaBI TAC'GTA Cuts at: 493 3978 493 Size: 3485 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6187 SphI G_CATG'C Cuts at: 1132 3545 1132 Size: 2413 3774 SspI AAT'ATT Cuts at: 5 52 4111 5 Size: 47 4059 2081 Fragments arranged by size: 4059 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6187 StyI C'CwwG_G Cuts at: 513 1065 2332 2490 3626 513 Size: 552 1267 158 1136 3074 Fragments arranged by size: 3074 1267 1136 552 158 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3310 4214 4231 4384 4569 5014 325 Size: 1255 1730 904 17 153 185 445 1498 Fragments arranged by size: 1730 1498 1255 904 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2774 3080 3271 Size: 274 80 33 51 1443 797 306 191 Cuts at: 3271 4433 6145 96 Size: 1162 1712 138 Fragments arranged by size: 1712 1443 1162 797 306 274 191 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6147 6179 6147 Size: 32 6155 Tth111I GACn'n_nGTC Cuts at: 1316 3365 1316 Size: 2049 4138 XbaI T'CTAG_A Cuts at: 3133 3523 3133 Size: 390 5797 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6187 XmaI C'CCGG_G Cuts at: 1870 3512 1870 Size: 1642 4545 XmnI GAAnn'nnTTC Cuts at: 4316 4316 Size: 6187 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3995 276 Size: 53 83 186 936 2461 2468 Fragments arranged by size: 2468 2461 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI