################################## # NO CUT ENZYMES ( 214 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmFI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssKI BssSI Bst1107I BstAPI BstBI BstEII BstNI BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I Cfr13I ClaI CpoI DpnII DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRII EcoRV EcoT22I FbaI FseI FspI HaeII HaeIII HapII HhaI HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MaeI MaeII MaeIII MamI MboI MfeI MflI MluI MluNI MroI MscI MslI MspI MunI MvaI MvnI NaeI NarI NciI NcoI NdeI NdeII NgoMIV NheI NlaIII NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsaI RsrII SacI SacII SalI SapI Sau3AI Sau96I SbfI ScaI ScrFI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tsp45I Tsp509I TspRI Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 28 enzymes) # ################################### AciI 264 GCGG CCGC(-3/-1) AcyI 94 GACGCC G[GA]^CG[CT]C AspI 289 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC BbsI 312 GAAGAC GAAGAC(2/6) BpuAI 312 GAAGAC GAAGAC(2/6) BsaHI 94 GACGCC G[GA]^CG[CT]C BsaJI 41 CCAAGG C^C[ACGT][ACGT]GG BsiYI 100 CCTGCTAACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 100 CCTGCTAACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 92 GAGAC GTCTC(1/5) BsmBI 92 GAGACG CGTCTC(1/5) BsmI 111 GAATGC GAATGC(1/-1) DdeI 245 CTGAG C^T[ACGT]AG EcoT14I 41 CCAAGG C^C[AT][AT]GG FauI 264 GCGGG CCCGC(4/6) Hin1I 94 GACGCC G[GA]^CG[CT]C HinfI 247 GAGTC G^A[ACGT]TC MseI 49 TTAA T^TAA MspA1I 269 CAGCTG C[AC]G^C[GT]G MwoI 244 GCTGAGTCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC PflFI 289 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 247 GAGTC GAGTC(4/5) PvuII 269 CAGCTG CAG^CTG StyI 41 CCAAGG C^C[AT][AT]GG TaqI 296 TCGA T^CGA Tru9I 49 TTAA T^TAA Tth111I 289 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 296 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 8 enzymes) # ################################### AccI 16 GTAGAC GT^[AC][GT]AC 154 GTAGAC BbvI 10 GCAGC GCAGC(8/12) 268 GCAGC BseRI 187 GAGGAG GAGGAG(10/8) 190 GAGGAG Cac8I 260 GCAAGC GC[ACGT]^[ACGT]GC 264 GCGGGC Fnu4HI 10 GCAGC GC^[ACGT]GC 268 GCAGC HgaI 7 GACGC GACGC(5/10) 94 GACGC ItaI 10 GCAGC GC^[ACGT]GC 268 GCAGC TseI 10 GCAGC G^C[AT]GC 268 GCAGC ######################################### # 3 OR MORE CUT ENZYMES ( 8 enzymes) # ######################################### AluI BstF5I EarI FokI HphI Ksp632I MboII MnlI