Restriction map for
pFN21AB6962
(Circular) (Six-base) MAPSORT of: pFN21AB6962.seq Check: 8414 from: 1 to: 5425
pFN21AB6962 5425 bp
With 182 enzymes: SgfI *
April 15, 2010 09:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3235 278
Size: 53 83 186 936 1699 2468
Fragments arranged by size:
2468 1699 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2223 2497 2538 2768 1937
Size: 286 274 41 230 4594
Fragments arranged by size:
4594 286 274 230 41
Acc65I G'GTAC_C
Cuts at: 2746 2746
Size: 5425
AclI AA'CG_TT
Cuts at: 1563 3552 3925 1563
Size: 1989 373 3063
Fragments arranged by size:
3063 1989 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3488 4872 5140 1632
Size: 1856 1384 268 1917
Fragments arranged by size:
1917 1856 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5228
AflIII A'CryG_T
Cuts at: 1203 4324 5301 1203
Size: 3121 977 1327
Fragments arranged by size:
3121 1327 977
AgeI A'CCGG_T
Cuts at: 4256 4256
Size: 5425
AhdI GACnn_n'nnGTC
Cuts at: 4154 4154
Size: 5425
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5425
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2684 1184
Size: 1500 3925
AlwNI CAG_nnn'CTG
Cuts at: 2381 3168 4740 2381
Size: 787 1572 3066
Fragments arranged by size:
3066 1572 787
ApaI G_GGCC'C
Cuts at: 2489 2489
Size: 5425
ApaLI G'TGCA_C
Cuts at: 3482 4638 3482
Size: 1156 4269
ApoI r'AATT_y
Cuts at: 1493 2734 2960 3112 5221 1493
Size: 1241 226 152 2109 1697
Fragments arranged by size:
2109 1697 1241 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3979 160
Size: 872 2947 1606
Fragments arranged by size:
2947 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5425
AvaI C'yCGr_G
Cuts at: 1870 1943 2750 1870
Size: 73 807 4545
Fragments arranged by size:
4545 807 73
BamHI G'GATC_C
Cuts at: 2755 3122 2755
Size: 367 5058
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2075 2088 2278 2429 2746
Size: 325 217 627 288 13 190 151 317
Cuts at: 2746 4201 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 325 317 288 217 190
151 13
BanII G_rGCy'C
Cuts at: 1391 2489 2551 2744 1391
Size: 1098 62 193 4072
Fragments arranged by size:
4072 1098 193 62
BbeI G_GCGC'C
Cuts at: 2079 2282 2079
Size: 203 5222
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4454
BbvCI CC'TCA_GC
Cuts at: 2184 2184
Size: 5425
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2295 2329 2619 2653 3616 3650 1903
Size: 34 358 34 290 34 963 34 3678
Fragments arranged by size:
3678 963 358 290 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3317 4527 3317
Size: 1210 4215
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5425
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2073 4036 136
Size: 107 122 71 631 605 401 1963 1525
Fragments arranged by size:
1963 1525 631 605 401 122 107 71
BglII A'GATC_T
Cuts at: 5420 5420
Size: 5425
BlpI GC'TnA_GC
Cuts at: 2842 2842
Size: 5425
Bme1580I G_kGCm'C
Cuts at: 1790 2093 2489 2568 3486 4642 1790
Size: 303 396 79 918 1156 2573
Fragments arranged by size:
2573 1156 918 396 303 79
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5425
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2721 3156 4114
Size: 416 53 83 399 24 1304 435 958
Cuts at: 4114 442
Size: 1753
Fragments arranged by size:
1753 1304 958 435 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2048 1056
Size: 992 4433
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2510 2664 4085 1239
Size: 1271 154 1421 2579
Fragments arranged by size:
2579 1421 1271 154
Bpu10I CC'TnA_GC
Cuts at: 2184 2184
Size: 5425
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3550 4415 4713 3550
Size: 865 298 4262
Fragments arranged by size:
4262 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2403 2682 4088 882
Size: 617 904 279 1406 2219
Fragments arranged by size:
2219 1406 904 617 279
BsaAI yAC'GTr
Cuts at: 493 1204 2563 3216 493
Size: 711 1359 653 2702
Fragments arranged by size:
2702 1359 711 653
BsaBI GATnn'nnATC
Cuts at: 5419 5419
Size: 5425
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2076 2279 3232 3614
Size: 53 83 186 936 543 203 953 382
Cuts at: 3614 4249 275
Size: 635 1451
Fragments arranged by size:
1451 953 936 635 543 382 203 186
83 53
BsaWI w'CCGG_w
Cuts at: 2592 3858 4256 4530 4677 5088 2592
Size: 1266 398 274 147 411 2929
Fragments arranged by size:
2929 1266 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5200
Fragments arranged by size:
5200 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2156 2159 2360 2602 2689 1186
Size: 970 3 201 242 87 3922
Fragments arranged by size:
3922 970 242 201 87 3
BseYI C'CCAG_C
Cuts at: 2448 4628 5124 2448
Size: 2180 496 2749
Fragments arranged by size:
2749 2180 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2132 1163
Size: 573 396 4456
Fragments arranged by size:
4456 573 396
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2305 2875 3636 4664 1540
Size: 275 186 304 570 761 1028 2301
Fragments arranged by size:
2301 1028 761 570 304 275 186
BsiHKAI G_wGCw'C
Cuts at: 3486 3571 4642 3486
Size: 85 1071 4269
Fragments arranged by size:
4269 1071 85
BsmI GAATG_Cn'
Cuts at: 2936 3029 2936
Size: 93 5332
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2093 2489 2551 2568 2744 3486 3571
Size: 399 303 396 62 17 176 742 85
Cuts at: 3571 4642 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 396 303 176 85
62 17
BspEI T'CCGG_A
Cuts at: 5088 5088
Size: 5425
BspHI T'CATG_A
Cuts at: 3314 5044 3314
Size: 1730 3695
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2780 844
Size: 1936 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2610 3312 1394
Size: 429 787 702 3507
Fragments arranged by size:
3507 787 702 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2695 3920 4094 65
Size: 1183 1447 1225 174 1396
Fragments arranged by size:
1447 1396 1225 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4069 4256 1700
Size: 110 2259 187 2869
Fragments arranged by size:
2869 2259 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5425
BssHII G'CGCG_C
Cuts at: 1925 2328 1925
Size: 403 5022
BssSI C'ACGA_G
Cuts at: 3485 4497 3485
Size: 1012 4413
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2110 2381 794
Size: 1316 271 3838
Fragments arranged by size:
3838 1316 271
BstBI TT'CG_AA
Cuts at: 4229 4229
Size: 5425
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5425
BstYI r'GATC_y
Cuts at: 1972 2755 3122 3507 3524 4976 5062 5074 5420
Size: 783 367 385 17 1452 86 12 346
Cuts at: 5420 1972
Size: 1977
Fragments arranged by size:
1977 1452 783 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2355 2355
Size: 5425
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4873
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4686
Fragments arranged by size:
4686 683 56
BtsI GCAGTG_nn'
Cuts at: 2258 2379 2609 2941 3735 3755 2258
Size: 121 230 332 794 20 3928
Fragments arranged by size:
3928 794 332 230 121 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5425
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5390
DraI TTT'AAA
Cuts at: 2729 2729
Size: 5425
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4432 809
Size: 507 3116 1802
Fragments arranged by size:
3116 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2302 2872 3761
Size: 54 1425 50 275 18 472 570 889
Cuts at: 3761 5390 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 570 472 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2302 2872 1537
Size: 275 490 570 4090
Fragments arranged by size:
4090 570 490 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3355 1353
Size: 2002 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2250 4012 4386 4532 387
Size: 1064 138 661 1762 374 146 1280
Fragments arranged by size:
1762 1280 1064 661 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2510 2664 3488 4085 4872 5140 1239
Size: 393 878 154 824 597 787 268 1524
Fragments arranged by size:
1524 878 824 787 597 393 268 154
EcoO109I rG'GnC_Cy
Cuts at: 2633 2633
Size: 5425
EcoRI G'AATT_C
Cuts at: 2734 3112 2734
Size: 378 5047
FspI TGC'GCA
Cuts at: 3931 3931
Size: 5425
HaeII r_GCGC'y
Cuts at: 1678 2079 2282 2294 2629 4572 1678
Size: 401 203 12 335 1943 2531
Fragments arranged by size:
2531 1943 401 335 203 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2141 2173 4066 4098 4140
Size: 32 135 32 569 32 1893 32 42
Cuts at: 4140 4172 1373
Size: 32 2626
Fragments arranged by size:
2626 1893 569 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2769 3015 669
Size: 988 281 831 246 3079
Fragments arranged by size:
3079 988 831 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5425
HpaI GTT'AAC
Cuts at: 3015 3015
Size: 5425
KasI G'GCGC_C
Cuts at: 2075 2278 2075
Size: 203 5222
KpnI G_GTAC'C
Cuts at: 2750 2750
Size: 5425
MfeI C'AATT_G
Cuts at: 2569 3024 2569
Size: 455 4970
MluI A'CGCG_T
Cuts at: 5301 5301
Size: 5425
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4539 4723 1305
Size: 3234 184 2007
Fragments arranged by size:
3234 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3603
Fragments arranged by size:
3603 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2480 3383 3742 3901 518
Size: 648 1314 903 359 159 2042
Fragments arranged by size:
2042 1314 903 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2032 2313 2841 3518 4666 4911 1606
Size: 426 281 528 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 528 426 281 245
NarI GG'CG_CC
Cuts at: 2076 2279 2076
Size: 203 5222
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4873
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5425
NheI G'CTAG_C
Cuts at: 1052 2044 1052
Size: 992 4433
NotI GC'GGCC_GC
Cuts at: 2872 2872
Size: 5425
NspI r_CATG'y
Cuts at: 1132 1229 2783 4328 1132
Size: 97 1554 1545 2229
Fragments arranged by size:
2229 1554 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2617 1348
Size: 1269 4156
PciI A'CATG_T
Cuts at: 4324 4324
Size: 5425
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5425
PmeI GTTT'AAAC
Cuts at: 2729 2729
Size: 5425
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3494 3526 5007 5039 3494
Size: 32 1481 32 3880
Fragments arranged by size:
3880 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2633 2633
Size: 5425
PshAI GACnn'nnGTC
Cuts at: 1613 2534 1613
Size: 921 4504
PsiI TTA'TAA
Cuts at: 2995 2995
Size: 5425
PspOMI G'GGCC_C
Cuts at: 2485 2485
Size: 5425
PstI C_TGCA'G
Cuts at: 830 2253 2520 2777 830
Size: 1423 267 257 3478
Fragments arranged by size:
3478 1423 267 257
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5425
SalI G'TCGA_C
Cuts at: 1936 2767 1936
Size: 831 4594
SbfI CC_TGCA'GG
Cuts at: 2777 2777
Size: 5425
ScaI AGT'ACT
Cuts at: 3673 5385 3673
Size: 1712 3713
SfcI C'TryA_G
Cuts at: 826 1045 2249 2516 2773 3908 4589 4780 826
Size: 219 1204 267 257 1135 681 191 1471
Fragments arranged by size:
1471 1204 1135 681 267 257 219 191
SfoI GGC'GCC
Cuts at: 2077 2280 2077
Size: 203 5222
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5425
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5425
SmaI CCC'GGG
Cuts at: 1872 2752 1872
Size: 880 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3529 4430 4692 820
Size: 197 926 1586 901 262 1553
Fragments arranged by size:
1586 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3216 493
Size: 2723 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5425
SphI G_CATG'C
Cuts at: 1132 2783 1132
Size: 1651 3774
SspI AAT'ATT
Cuts at: 5 52 3349 5
Size: 47 3297 2081
Fragments arranged by size:
3297 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5425
StyI C'CwwG_G
Cuts at: 513 1065 2079 2265 2864 513
Size: 552 1014 186 599 3074
Fragments arranged by size:
3074 1014 599 552 186
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3452 3469 3622 3807 4252 325
Size: 1255 1872 17 153 185 445 1498
Fragments arranged by size:
1872 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3671 5383 96
Size: 274 80 33 51 1443 1694 1712 138
Fragments arranged by size:
1712 1694 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5385 5417 5385
Size: 32 5393
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5425
XbaI T'CTAG_A
Cuts at: 2761 2761
Size: 5425
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5425
XmaI C'CCGG_G
Cuts at: 1870 2750 1870
Size: 880 4545
XmnI GAAnn'nnTTC
Cuts at: 3554 3554
Size: 5425
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3233 276
Size: 53 83 186 936 1699 2468
Fragments arranged by size:
2468 1699 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV
FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI
PfoI PmlI PspXI PsrI PvuII RsrII SacI SacII
SanDI SapI SexAI SfiI SrfI SwaI XcmI