################################## # NO CUT ENZYMES ( 123 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspHI AspI AviII AvrII BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsiHKAI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI BssHII BssSI BstBI BstEII BstPI CelII ClaI DraI DraIII Eam1105I EcoO65I EcoRI EcoT14I EcoT22I FbaI FseI FspI HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI MaeII MamI MfeI MluI MroI MslI MunI NarI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI SacI SalI SbfI ScaI SexAI SfoI SfuI SgrAI SmlI SnaBI SphI Sse8387I SspBI SspI StyI SwaI TfiI Tsp509I Tth111I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AccI 640 GTATAC GT^[AC][GT]AC AfaI 629 GTAC GT^AC AocI 427 CCTCAGG CC^T[ACGT]AGG Aor51HI 365 AGCGCT AGC^GCT BamHI 318 GGATCC G^GATCC BanI 90 GGTGCC G^G[CT][GA]CC BsaI 655 GAGACC GGTCTC(1/5) BsgI 1321 CTGCAC GTGCAG(16/14) BspMI 87 GCAGGT ACCTGC(4/8) Bst1107I 640 GTATAC GTA^TAC BstAPI 864 GCAACTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 587 CCAGCCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 640 GTATAC GTA^TAC Bsu36I 427 CCTCAGG CC^T[ACGT]AGG CpoI 104 CGGTCCG CG^G[AT]CCG DrdI 544 GACTGCCTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 122 CGGCCG C^GGCCG EclXI 122 CGGCCG C^GGCCG Eco47III 365 AGCGCT AGC^GCT Eco52I 122 CGGCCG C^GGCCG Eco81I 427 CCTCAGG CC^T[ACGT]AGG EcoNI 555 CCTTAACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 1161 GATATC GAT^ATC HphI 1002 GGTGA GGTGA(8/7) KspI 263 CCGCGG CCGC^GG MaeIII 542 GTGAC ^GT[ACGT]AC NaeI 124 GCCGGC GCC^GGC NgoMIV 124 GCCGGC G^CCGGC PstI 508 CTGCAG CTGCA^G RsaI 629 GTAC GT^AC RsrII 104 CGGTCCG CG^G[AT]CCG SacII 263 CCGCGG CCGC^GG SapI 466 GAAGAGC GCTCTTC(1/4) SpeI 18 ACTAGT A^CTAGT StuI 1295 AGGCCT AGG^CCT Tsp45I 542 GTGAC ^GT[GC]AC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### BalI 356 TGGCCA TGG^CCA 647 TGGCCA BbsI 448 GTCTTC GAAGAC(2/6) 1377 GTCTTC BglII 1143 AGATCT A^GATCT 1277 AGATCT BpuAI 448 GTCTTC GAAGAC(2/6) 1377 GTCTTC BsiEI 122 CGGCCG CG[GA][CT]^CG 162 CGACCG BsmBI 7 GAGACG CGTCTC(1/5) 1354 GAGACG BsrDI 441 CATTGC GCAATG(2/0) 929 GCAATG BsrFI 124 GCCGGC [GA]^CCGG[CT] 860 ACCGGC BstF5I 909 CATCC GGATG(2/0) 1063 CATCC Cfr10I 124 GCCGGC [GA]^CCGG[CT] 860 ACCGGC DsaI 227 CCGTGG C^C[GA][CT]GG 263 CCGCGG FauI 33 GCGGG CCCGC(4/6) 1269 GCGGG FokI 909 CATCC GGATG(9/13) 1063 CATCC HaeII 73 GGCGCT [GA]GCGC^[CT] 365 AGCGCT MluNI 356 TGGCCA TGG^CCA 647 TGGCCA MscI 356 TGGCCA TGG^CCA 647 TGGCCA NlaIII 1266 CATG CATG^ 1430 CATG PpuMI 422 AGGACCC [GA]G^G[AT]CC[CT] 1196 AGGACCT SfcI 508 CTGCAG C^T[GA][CT]AG 666 CTATAG SfiI 252 GGCCGCTCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 348 GGCCGAGCTGGCC SmaI 94 CCCGGG CCC^GGG 194 CCCGGG XmaCI 94 CCCGGG C^CCGGG 194 CCCGGG XmaI 94 CCCGGG C^CCGGG 194 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 76 enzymes) # ######################################### AccII AciI AluI AlwI AlwNI ApaI Asp700I AvaI AvaII BanII BbvI BcnI BfaI BglI BmyI BpmI BsaJI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrBI BsrI BssKI BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EarI EcoO109I EcoRII Fnu4HI HaeIII HapII HhaI HinP1I HinfI HpaII ItaI Ksp632I MaeI MboI MboII MflI MnlI MseI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIV PflMI PleI Sau3AI Sau96I ScrFI SfaNI TaqI Tru9I TseI TspRI TthHB8I Van91I XhoII XmnI