(Circular) (Six-base) MAPSORT of: pFN21AE3606.seq Check: 2417 from: 1 to: 6142 pFN21AE3606 6142 bp With 182 enzymes: SgfI * December 8, 2014 13:40 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2083 2083 Size: 6142 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3952 278 Size: 53 83 186 936 2416 2468 Fragments arranged by size: 2468 2416 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2646 3485 1937 Size: 709 839 4594 Fragments arranged by size: 4594 839 709 Acc65I G'GTAC_C Cuts at: 3463 3463 Size: 6142 AclI AA'CG_TT Cuts at: 1563 4269 4642 1563 Size: 2706 373 3063 Fragments arranged by size: 3063 2706 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3083 4205 5589 5857 1632 Size: 1451 1122 1384 268 1917 Fragments arranged by size: 1917 1451 1384 1122 268 AfeI AGC'GCT Cuts at: 2372 2372 Size: 6142 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5945 AflIII A'CryG_T Cuts at: 1203 5041 6018 1203 Size: 3838 977 1327 Fragments arranged by size: 3838 1327 977 AgeI A'CCGG_T Cuts at: 4973 4973 Size: 6142 AhdI GACnn_n'nnGTC Cuts at: 4871 4871 Size: 6142 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6142 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6142 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2315 2347 2315 Size: 32 6110 AlwNI CAG_nnn'CTG Cuts at: 2185 2254 2521 3885 5457 2185 Size: 69 267 1364 1572 2870 Fragments arranged by size: 2870 1572 1364 267 69 ApaI G_GGCC'C Cuts at: 2044 2141 2200 2044 Size: 97 59 5986 Fragments arranged by size: 5986 97 59 ApaLI G'TGCA_C Cuts at: 4199 5355 4199 Size: 1156 4986 ApoI r'AATT_y Cuts at: 1493 3451 3677 3829 5938 1493 Size: 1958 226 152 2109 1697 Fragments arranged by size: 2109 1958 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4696 160 Size: 872 3664 1606 Fragments arranged by size: 3664 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6142 AvaI C'yCGr_G Cuts at: 1870 1943 2033 2074 2099 2134 2199 2384 2985 Size: 73 90 41 25 35 65 185 601 Cuts at: 2985 3467 1870 Size: 482 4545 Fragments arranged by size: 4545 601 482 185 90 73 65 41 35 25 BamHI G'GATC_C Cuts at: 2323 3472 3839 2323 Size: 1149 367 4626 Fragments arranged by size: 4626 1149 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2095 3463 4918 618 Size: 325 217 627 308 1368 1455 1842 Fragments arranged by size: 1842 1455 1368 627 325 308 217 BanII G_rGCy'C Cuts at: 1391 2021 2044 2075 2141 2156 2200 2405 2478 Size: 630 23 31 66 15 44 205 73 Cuts at: 2478 3461 1391 Size: 983 4072 Fragments arranged by size: 4072 983 630 205 73 66 44 31 23 15 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2446 3375 928 Size: 971 547 929 3695 Fragments arranged by size: 3695 971 929 547 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4333 4367 1903 Size: 34 2396 34 3678 Fragments arranged by size: 3678 2396 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4034 5244 4034 Size: 1210 4932 BclI T'GATC_A Cuts at: 1548 1548 Size: 6142 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2264 2273 2291 Size: 107 122 71 631 605 592 9 18 Cuts at: 2291 2360 2482 4753 136 Size: 69 122 2271 1525 Fragments arranged by size: 2271 1525 631 605 592 122 122 107 71 69 18 9 BglII A'GATC_T Cuts at: 3148 3282 6137 3148 Size: 134 2855 3153 Fragments arranged by size: 3153 2855 134 BlpI GC'TnA_GC Cuts at: 3559 3559 Size: 6142 Bme1580I G_kGCm'C Cuts at: 1790 2044 2100 2141 2200 4203 5359 1790 Size: 254 56 41 59 2003 1156 2573 Fragments arranged by size: 2573 2003 1156 254 59 56 41 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6142 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3007 3109 3873 Size: 416 53 83 399 24 1590 102 764 Cuts at: 3873 4831 442 Size: 958 1753 Fragments arranged by size: 1753 1590 958 764 416 399 102 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6142 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2389 2864 3100 4802 1239 Size: 1150 475 236 1702 2579 Fragments arranged by size: 2579 1702 1150 475 236 Bpu10I CC'TnA_GC Cuts at: 2262 2289 2262 Size: 27 6115 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4267 5132 5430 4267 Size: 865 298 4979 Fragments arranged by size: 4979 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2654 4805 882 Size: 617 1155 2151 2219 Fragments arranged by size: 2219 2151 1155 617 BsaAI yAC'GTr Cuts at: 493 1204 3933 493 Size: 711 2729 2702 Fragments arranged by size: 2729 2702 711 BsaBI GATnn'nnATC Cuts at: 6136 6136 Size: 6142 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3949 4331 4966 275 Size: 53 83 186 936 2416 382 635 1451 Fragments arranged by size: 2416 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4575 4973 5247 5394 5805 4575 Size: 398 274 147 411 4912 Fragments arranged by size: 4912 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2387 2417 2862 2892 1178 Size: 30 165 30 984 30 445 30 4428 Fragments arranged by size: 4428 984 445 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6142 BseYI C'CCAG_C Cuts at: 5345 5841 5345 Size: 496 5646 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3311 1163 Size: 573 1575 3994 Fragments arranged by size: 3994 1575 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2130 2170 3592 4353 5381 1540 Size: 275 186 129 40 1422 761 1028 2301 Fragments arranged by size: 2301 1422 1028 761 275 186 129 40 BsiHKAI G_wGCw'C Cuts at: 4203 4288 5359 4203 Size: 85 1071 4986 Fragments arranged by size: 4986 1071 85 BsmI GAATG_Cn' Cuts at: 3653 3746 3653 Size: 93 6049 BsmBI CGTCTCn'nnnn_ Cuts at: 2006 3353 2006 Size: 1347 4795 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2021 2044 2075 2100 2141 2156 2200 Size: 399 231 23 31 25 41 15 44 Cuts at: 2200 2405 2478 3461 4203 4288 5359 1391 Size: 205 73 983 742 85 1071 2174 Fragments arranged by size: 2174 1071 983 742 399 231 205 85 73 44 41 31 25 23 15 BspEI T'CCGG_A Cuts at: 5805 5805 Size: 6142 BspHI T'CATG_A Cuts at: 4031 5761 4031 Size: 1730 4412 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2083 3497 844 Size: 1239 1414 3489 Fragments arranged by size: 3489 1414 1239 BsrBI CCG'CTC Cuts at: 1394 1823 2038 2176 2261 2288 2305 2890 4029 Size: 429 215 138 85 27 17 585 1139 Cuts at: 4029 1394 Size: 3507 Fragments arranged by size: 3507 1139 585 429 215 138 85 27 17 BsrDI GCAATG_nn' Cuts at: 65 1248 2445 2941 4637 4811 65 Size: 1183 1197 496 1696 174 1396 Fragments arranged by size: 1696 1396 1197 1183 496 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2129 2865 4786 4973 1700 Size: 110 319 736 1921 187 2869 Fragments arranged by size: 2869 1921 736 319 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6142 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6142 BssSI C'ACGA_G Cuts at: 4202 5214 4202 Size: 1012 5130 BstAPI GCAn_nnn'nTGC Cuts at: 794 2875 794 Size: 2081 4061 BstBI TT'CG_AA Cuts at: 4946 4946 Size: 6142 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6142 BstXI CCAn_nnnn'nTGG Cuts at: 2599 2599 Size: 6142 BstYI r'GATC_y Cuts at: 1972 2323 3148 3226 3282 3472 3839 4224 4241 Size: 351 825 78 56 190 367 385 17 Cuts at: 4241 5693 5779 5791 6137 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 825 385 367 351 346 190 86 78 56 17 12 BstZ17I GTA'TAC Cuts at: 2647 2647 Size: 6142 Bsu36I CC'TnA_GG Cuts at: 2433 2433 Size: 6142 BtgI C'CryG_G Cuts at: 513 1065 2004 2232 2268 513 Size: 552 939 228 36 4387 Fragments arranged by size: 4387 939 552 228 36 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5403 Fragments arranged by size: 5403 683 56 BtsI GCAGTG_nn' Cuts at: 3658 4452 4472 3658 Size: 794 20 5328 Fragments arranged by size: 5328 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6142 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6107 DraI TTT'AAA Cuts at: 3446 3446 Size: 6142 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2555 5149 809 Size: 507 1239 2594 1802 Fragments arranged by size: 2594 1802 1239 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2127 2256 2361 Size: 54 1425 50 275 18 297 129 105 Cuts at: 2361 2542 2652 3589 4478 6107 8 Size: 181 110 937 889 1629 43 Fragments arranged by size: 1629 1425 937 889 297 275 181 129 110 105 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2127 3589 1537 Size: 275 315 1462 4090 Fragments arranged by size: 4090 1462 315 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2466 2853 3192 3221 4072 1353 Size: 1113 387 339 29 851 3423 Fragments arranged by size: 3423 1113 851 387 339 29 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2136 4729 5103 5249 387 Size: 1064 138 547 2593 374 146 1280 Fragments arranged by size: 2593 1280 1064 547 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2389 2864 3083 3100 4205 4802 5589 Size: 393 757 475 219 17 1105 597 787 Cuts at: 5589 5857 1239 Size: 268 1524 Fragments arranged by size: 1524 1105 787 757 597 475 393 268 219 17 EcoNI CCTnn'n_nnAGG Cuts at: 2564 2564 Size: 6142 EcoO109I rG'GnC_Cy Cuts at: 2138 2428 3202 2138 Size: 290 774 5078 Fragments arranged by size: 5078 774 290 EcoRI G'AATT_C Cuts at: 3451 3829 3451 Size: 378 5764 EcoRV GAT'ATC Cuts at: 3168 3168 Size: 6142 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3445 3445 Size: 6142 FspI TGC'GCA Cuts at: 4648 4648 Size: 6142 HaeII r_GCGC'y Cuts at: 1678 2082 2374 5289 1678 Size: 404 292 2915 2531 Fragments arranged by size: 2915 2531 404 292 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3073 3105 3133 3165 3274 Size: 32 135 32 1501 32 28 32 109 Cuts at: 3274 3306 4783 4815 4857 4889 1373 Size: 32 1477 32 42 32 2626 Fragments arranged by size: 2626 1501 1477 135 109 42 32 32 32 32 32 32 32 28 HincII GTy'rAC Cuts at: 669 1657 1938 3486 3732 669 Size: 988 281 1548 246 3079 Fragments arranged by size: 3079 1548 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6142 HpaI GTT'AAC Cuts at: 3732 3732 Size: 6142 KpnI G_GTAC'C Cuts at: 3467 3467 Size: 6142 MfeI C'AATT_G Cuts at: 3741 3741 Size: 6142 MluI A'CGCG_T Cuts at: 6018 6018 Size: 6142 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2169 2716 5256 5440 1305 Size: 864 547 2540 184 2007 Fragments arranged by size: 2540 2007 864 547 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2363 2654 10 Size: 54 1425 343 531 291 3498 Fragments arranged by size: 3498 1425 531 343 291 54 MslI CAynn'nnrTG Cuts at: 518 1166 4100 4459 4618 518 Size: 648 2934 359 159 2042 Fragments arranged by size: 2934 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2270 2409 2743 3379 3558 4235 5383 5628 Size: 664 139 334 636 179 677 1148 245 Cuts at: 5628 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 664 636 334 245 179 139 NaeI GCC'GGC Cuts at: 2131 2131 Size: 6142 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4651 Fragments arranged by size: 4651 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6142 NgoMIV G'CCGG_C Cuts at: 2129 2129 Size: 6142 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6142 NotI GC'GGCC_GC Cuts at: 3589 3589 Size: 6142 NspI r_CATG'y Cuts at: 1132 1229 3500 5045 1132 Size: 97 2271 1545 2229 Fragments arranged by size: 2271 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2215 1348 Size: 867 5275 PciI A'CATG_T Cuts at: 5041 5041 Size: 6142 PflMI CCAn_nnn'nTGG Cuts at: 1108 2233 2254 2611 2735 2774 1108 Size: 1125 21 357 124 39 4476 Fragments arranged by size: 4476 1125 357 124 39 21 PfoI T'CCnGG_A Cuts at: 2316 3077 2316 Size: 761 5381 PmeI GTTT'AAAC Cuts at: 3446 3446 Size: 6142 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4211 4243 5724 5756 4211 Size: 32 1481 32 4597 Fragments arranged by size: 4597 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2428 3202 2428 Size: 774 5368 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6142 PsiI TTA'TAA Cuts at: 3712 3712 Size: 6142 PspOMI G'GGCC_C Cuts at: 2040 2137 2196 2040 Size: 97 59 5986 Fragments arranged by size: 5986 97 59 PstI C_TGCA'G Cuts at: 830 2517 3494 830 Size: 1687 977 3478 Fragments arranged by size: 3478 1687 977 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6142 RsrII CG'GwC_CG Cuts at: 2110 2110 Size: 6142 SacII CC_GC'GG Cuts at: 2271 2271 Size: 6142 SalI G'TCGA_C Cuts at: 1936 3484 1936 Size: 1548 4594 SapI GCTCTTCn'nnn_ Cuts at: 2466 2466 Size: 6142 SbfI CC_TGCA'GG Cuts at: 3494 3494 Size: 6142 ScaI AGT'ACT Cuts at: 4390 6102 4390 Size: 1712 4430 SfcI C'TryA_G Cuts at: 826 1045 2513 2671 3490 4625 5306 5497 826 Size: 219 1468 158 819 1135 681 191 1471 Fragments arranged by size: 1471 1468 1135 819 681 219 191 158 SfiI GGCCn_nnn'nGGCC Cuts at: 2264 2360 2264 Size: 96 6046 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6142 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6142 SmaI CCC'GGG Cuts at: 1872 2101 2201 3469 1872 Size: 229 100 1268 4545 Fragments arranged by size: 4545 1268 229 100 SmlI C'TyrA_G Cuts at: 820 1017 1943 4246 5147 5409 820 Size: 197 926 2303 901 262 1553 Fragments arranged by size: 2303 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3933 493 Size: 3440 2702 SpeI A'CTAG_T Cuts at: 152 2023 152 Size: 1871 4271 SphI G_CATG'C Cuts at: 1132 3500 1132 Size: 2368 3774 SspI AAT'ATT Cuts at: 5 52 4066 5 Size: 47 4014 2081 Fragments arranged by size: 4014 2081 47 StuI AGG'CCT Cuts at: 1517 3302 1517 Size: 1785 4357 StyI C'CwwG_G Cuts at: 513 1065 2004 3581 513 Size: 552 939 1577 3074 Fragments arranged by size: 3074 1577 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2700 2989 4169 4186 4339 4524 4969 Size: 1255 1120 289 1180 17 153 185 445 Cuts at: 4969 325 Size: 1498 Fragments arranged by size: 1498 1255 1180 1120 445 289 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4388 6100 96 Size: 274 80 33 51 1443 2411 1712 138 Fragments arranged by size: 2411 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6102 6134 6102 Size: 32 6110 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6142 XbaI T'CTAG_A Cuts at: 3478 3478 Size: 6142 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6142 XmaI C'CCGG_G Cuts at: 1870 2099 2199 3467 1870 Size: 229 100 1268 4545 Fragments arranged by size: 4545 1268 229 100 XmnI GAAnn'nnTTC Cuts at: 2312 2847 3425 4271 2312 Size: 535 578 846 4183 Fragments arranged by size: 4183 846 578 535 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3950 276 Size: 53 83 186 936 2416 2468 Fragments arranged by size: 2468 2416 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI RsrII SacII SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI DraIII EcoICRI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI PvuII SacI SanDI SexAI SfoI SrfI SwaI XcmI