(Circular) (Six-base) MAPSORT of: pFN21AB5730.seq Check: 4624 from: 1 to: 6901 pFN21AB5730 6901 bp With 182 enzymes: SgfI * April 15, 2010 09:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2045 3421 2045 Size: 1376 5525 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3621 4711 278 Size: 53 83 186 936 2085 1090 2468 Fragments arranged by size: 2468 2085 1090 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4244 1937 Size: 2307 4594 Acc65I G'GTAC_C Cuts at: 4222 4222 Size: 6901 AclI AA'CG_TT Cuts at: 1563 5028 5401 1563 Size: 3465 373 3063 Fragments arranged by size: 3465 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2299 2608 3432 3482 3857 4964 6348 6616 Size: 667 309 824 50 375 1107 1384 268 Cuts at: 6616 1632 Size: 1917 Fragments arranged by size: 1917 1384 1107 824 667 375 309 268 50 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6704 AflIII A'CryG_T Cuts at: 1203 3039 5800 6777 1203 Size: 1836 2761 977 1327 Fragments arranged by size: 2761 1836 1327 977 AgeI A'CCGG_T Cuts at: 5732 5732 Size: 6901 AhdI GACnn_n'nnGTC Cuts at: 2953 5630 2953 Size: 2677 4224 AleI CACnn'nnGTG Cuts at: 1166 2291 1166 Size: 1125 5776 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6901 AlwNI CAG_nnn'CTG Cuts at: 2432 2501 2860 3178 4188 4644 6216 2432 Size: 69 359 318 1010 456 1572 3117 Fragments arranged by size: 3117 1572 1010 456 359 318 69 ApaI G_GGCC'C Cuts at: 3460 4116 3460 Size: 656 6245 ApaLI G'TGCA_C Cuts at: 4155 4958 6114 4155 Size: 803 1156 4942 Fragments arranged by size: 4942 1156 803 ApoI r'AATT_y Cuts at: 1493 2413 4210 4436 4588 6697 1493 Size: 920 1797 226 152 2109 1697 Fragments arranged by size: 2109 1797 1697 920 226 152 AseI AT'TA_AT Cuts at: 160 1032 5455 160 Size: 872 4423 1606 Fragments arranged by size: 4423 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6901 AvaI C'yCGr_G Cuts at: 1870 1943 3301 4226 1870 Size: 73 1358 925 4545 Fragments arranged by size: 4545 1358 925 73 AvrII C'CTAG_G Cuts at: 2635 2635 Size: 6901 BamHI G'GATC_C Cuts at: 4231 4598 4231 Size: 367 6534 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2421 3969 4222 5677 618 Size: 325 217 627 634 1548 253 1455 1842 Fragments arranged by size: 1842 1548 1455 634 627 325 253 217 BanII G_rGCy'C Cuts at: 1391 2996 3264 3460 4116 4220 1391 Size: 1605 268 196 656 104 4072 Fragments arranged by size: 4072 1605 656 268 196 104 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5930 BbvCI CC'TCA_GC Cuts at: 4004 4118 4004 Size: 114 6787 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3238 3272 3499 3533 5092 5126 1903 Size: 34 1301 34 227 34 1559 34 3678 Fragments arranged by size: 3678 1559 1301 227 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2467 4793 6003 2467 Size: 2326 1210 3365 Fragments arranged by size: 3365 2326 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6901 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5512 136 Size: 107 122 71 631 605 3840 1525 Fragments arranged by size: 3840 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2727 2739 4017 6896 2727 Size: 12 1278 2879 2732 Fragments arranged by size: 2879 2732 1278 12 BlpI GC'TnA_GC Cuts at: 3167 4318 3167 Size: 1151 5750 Bme1580I G_kGCm'C Cuts at: 1790 2887 3460 4116 4159 4962 6118 1790 Size: 1097 573 656 43 803 1156 2573 Fragments arranged by size: 2573 1156 1097 803 656 573 43 BmgBI CAC'GTC Cuts at: 1318 3022 1318 Size: 1704 5197 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4632 5590 442 Size: 416 53 83 399 24 3215 958 1753 Fragments arranged by size: 3215 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3932 1056 Size: 2876 4025 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3015 3015 Size: 6901 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2076 2095 2423 2891 3111 3800 4165 5561 Size: 837 19 328 468 220 689 365 1396 Cuts at: 5561 1239 Size: 2579 Fragments arranged by size: 2579 1396 837 689 468 365 328 220 19 Bpu10I CC'TnA_GC Cuts at: 2990 3530 4004 4118 2990 Size: 540 474 114 5773 Fragments arranged by size: 5773 540 474 114 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2690 2909 5026 5891 6189 2690 Size: 219 2117 865 298 3402 Fragments arranged by size: 3402 2117 865 298 219 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2336 2787 3386 5564 882 Size: 617 837 451 599 2178 2219 Fragments arranged by size: 2219 2178 837 617 599 451 BsaAI yAC'GTr Cuts at: 493 1204 4160 4692 493 Size: 711 2956 532 2702 Fragments arranged by size: 2956 2702 711 532 BsaBI GATnn'nnATC Cuts at: 6895 6895 Size: 6901 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3618 4708 5090 5725 Size: 53 83 186 936 2085 1090 382 635 Cuts at: 5725 275 Size: 1451 Fragments arranged by size: 2085 1451 1090 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3540 5334 5732 6006 6153 6564 3540 Size: 1794 398 274 147 411 3877 Fragments arranged by size: 3877 1794 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3109 3139 1178 Size: 30 165 30 1706 30 4940 Fragments arranged by size: 4940 1706 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2453 2540 2805 3071 3254 3404 3662 1186 Size: 1267 87 265 266 183 150 258 4425 Fragments arranged by size: 4425 1267 266 265 258 183 150 87 BseYI C'CCAG_C Cuts at: 2773 2785 3992 4011 4058 4129 6104 6600 2773 Size: 12 1207 19 47 71 1975 496 3074 Fragments arranged by size: 3074 1975 1207 496 71 47 19 12 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2287 1163 Size: 573 551 5777 Fragments arranged by size: 5777 573 551 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4351 5112 6140 1540 Size: 275 186 2350 761 1028 2301 Fragments arranged by size: 2350 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2090 3308 4159 4168 4962 5047 6118 2090 Size: 1218 851 9 794 85 1071 2873 Fragments arranged by size: 2873 1218 1071 851 794 85 9 BsmI GAATG_Cn' Cuts at: 3913 4412 4505 3913 Size: 499 93 6309 Fragments arranged by size: 6309 499 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2090 2887 2996 3264 3308 3460 4116 Size: 399 300 797 109 268 44 152 656 Cuts at: 4116 4159 4168 4220 4962 5047 6118 1391 Size: 43 9 52 742 85 1071 2174 Fragments arranged by size: 2174 1071 797 742 656 399 300 268 152 109 85 52 44 43 9 BspEI T'CCGG_A Cuts at: 6564 6564 Size: 6901 BspHI T'CATG_A Cuts at: 4790 6520 4790 Size: 1730 5171 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2045 3421 4256 844 Size: 1201 1376 835 3489 Fragments arranged by size: 3489 1376 1201 835 BsrBI CCG'CTC Cuts at: 1394 1823 4788 1394 Size: 429 2965 3507 Fragments arranged by size: 3507 2965 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5396 5570 65 Size: 1183 4148 174 1396 Fragments arranged by size: 4148 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5545 5732 1700 Size: 110 3735 187 2869 Fragments arranged by size: 3735 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6901 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6901 BssSI C'ACGA_G Cuts at: 3008 3746 4961 5973 3008 Size: 738 1215 1012 3936 Fragments arranged by size: 3936 1215 1012 738 BstAPI GCAn_nnn'nTGC Cuts at: 794 4163 794 Size: 3369 3532 BstBI TT'CG_AA Cuts at: 4027 5705 4027 Size: 1678 5223 BstEII G'GTnAC_C Cuts at: 1184 3115 1184 Size: 1931 4970 BstXI CCAn_nnnn'nTGG Cuts at: 2057 2219 2057 Size: 162 6739 BstYI r'GATC_y Cuts at: 1972 2254 2727 2739 4017 4231 4598 4983 5000 Size: 282 473 12 1278 214 367 385 17 Cuts at: 5000 6452 6538 6550 6896 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1278 473 385 367 346 282 214 86 17 12 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2109 2126 3973 4108 513 Size: 552 939 105 17 1847 135 3306 Fragments arranged by size: 3306 1847 939 552 135 105 17 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3919 487 Size: 683 56 2693 3469 Fragments arranged by size: 3469 2693 683 56 BtsI GCAGTG_nn' Cuts at: 2139 3333 4417 5211 5231 2139 Size: 1194 1084 794 20 3809 Fragments arranged by size: 3809 1194 1084 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6901 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3578 3613 529 Size: 35 3014 35 3817 Fragments arranged by size: 3817 3014 35 35 DraI TTT'AAA Cuts at: 4205 4205 Size: 6901 DraIII CAC_nnn'GTG Cuts at: 4163 4163 Size: 6901 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5908 809 Size: 507 4592 1802 Fragments arranged by size: 4592 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2136 2608 4348 Size: 54 1425 50 275 18 306 472 1740 Cuts at: 4348 5237 6866 8 Size: 889 1629 43 Fragments arranged by size: 1740 1629 1425 889 472 306 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4348 1537 Size: 275 2536 4090 Fragments arranged by size: 4090 2536 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2025 2091 2824 2903 4831 1353 Size: 672 66 733 79 1928 3423 Fragments arranged by size: 3423 1928 733 672 79 66 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5488 5862 6008 387 Size: 1064 138 3899 374 146 1280 Fragments arranged by size: 3899 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2076 2095 2299 2423 2608 2891 3111 Size: 393 444 19 204 124 185 283 220 Cuts at: 3111 3432 3482 3800 3857 4165 4964 5561 6348 Size: 321 50 318 57 308 799 597 787 Cuts at: 6348 6616 1239 Size: 268 1524 Fragments arranged by size: 1524 799 787 597 444 393 321 318 308 283 268 220 204 185 124 57 50 19 EcoNI CCTnn'n_nnAGG Cuts at: 2329 4138 2329 Size: 1809 5092 EcoO109I rG'GnC_Cy Cuts at: 2639 2865 3457 4113 2639 Size: 226 592 656 5427 Fragments arranged by size: 5427 656 592 226 EcoRI G'AATT_C Cuts at: 2413 4210 4588 2413 Size: 1797 378 4726 Fragments arranged by size: 4726 1797 378 FspI TGC'GCA Cuts at: 5407 5407 Size: 6901 HaeII r_GCGC'y Cuts at: 1678 3726 3844 6048 1678 Size: 2048 118 2204 2531 Fragments arranged by size: 2531 2204 2048 118 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2939 2971 3109 3141 5542 Size: 32 135 32 1367 32 138 32 2401 Cuts at: 5542 5574 5616 5648 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2401 1367 138 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4245 4491 669 Size: 988 281 2307 246 3079 Fragments arranged by size: 3079 2307 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6901 HpaI GTT'AAC Cuts at: 4491 4491 Size: 6901 KpnI G_GTAC'C Cuts at: 4226 4226 Size: 6901 MfeI C'AATT_G Cuts at: 4500 4500 Size: 6901 MluI A'CGCG_T Cuts at: 6777 6777 Size: 6901 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3700 6015 6199 1305 Size: 2395 2315 184 2007 Fragments arranged by size: 2395 2315 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2138 2610 10 Size: 54 1425 343 306 472 4301 Fragments arranged by size: 4301 1425 472 343 306 54 MslI CAynn'nnrTG Cuts at: 518 1166 2245 2291 2981 4859 5218 5377 518 Size: 648 1079 46 690 1878 359 159 2042 Fragments arranged by size: 2042 1878 1079 690 648 359 159 46 MspA1I CmG'CkG Cuts at: 1606 2773 2878 3665 4152 4188 4317 4994 6142 Size: 1167 105 787 487 36 129 677 1148 Cuts at: 6142 6387 1606 Size: 245 2120 Fragments arranged by size: 2120 1167 1148 787 677 487 245 129 105 36 NcoI C'CATG_G Cuts at: 513 1065 2004 2126 3973 513 Size: 552 939 122 1847 3441 Fragments arranged by size: 3441 1847 939 552 122 NdeI CA'TA_TG Cuts at: 387 387 Size: 6901 NheI G'CTAG_C Cuts at: 1052 3928 1052 Size: 2876 4025 NotI GC'GGCC_GC Cuts at: 4348 4348 Size: 6901 NspI r_CATG'y Cuts at: 1132 1229 3043 4259 5804 1132 Size: 97 1814 1216 1545 2229 Fragments arranged by size: 2229 1814 1545 1216 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6901 PciI A'CATG_T Cuts at: 3039 5800 3039 Size: 2761 4140 PflMI CCAn_nnn'nTGG Cuts at: 1108 2432 2949 3022 3403 1108 Size: 1324 517 73 381 4606 Fragments arranged by size: 4606 1324 517 381 73 PfoI T'CCnGG_A Cuts at: 2259 3147 3342 3816 2259 Size: 888 195 474 5344 Fragments arranged by size: 5344 888 474 195 PmeI GTTT'AAAC Cuts at: 4205 4205 Size: 6901 PmlI CAC'GTG Cuts at: 4160 4160 Size: 6901 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4970 5002 6483 6515 4970 Size: 32 1481 32 5356 Fragments arranged by size: 5356 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2639 2639 Size: 6901 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6901 PsiI TTA'TAA Cuts at: 4471 4471 Size: 6901 PspOMI G'GGCC_C Cuts at: 3456 4112 3456 Size: 656 6245 PstI C_TGCA'G Cuts at: 830 2497 3090 3432 3522 3916 3945 4253 830 Size: 1667 593 342 90 394 29 308 3478 Fragments arranged by size: 3478 1667 593 394 342 308 90 29 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6901 PvuII CAG'CTG Cuts at: 2773 3665 2773 Size: 892 6009 SalI G'TCGA_C Cuts at: 1936 4243 1936 Size: 2307 4594 SapI GCTCTTCn'nnn_ Cuts at: 2025 2903 2025 Size: 878 6023 SbfI CC_TGCA'GG Cuts at: 4253 4253 Size: 6901 ScaI AGT'ACT Cuts at: 5149 6861 5149 Size: 1712 5189 SfcI C'TryA_G Cuts at: 826 1045 2493 3086 3428 3518 3912 3941 4249 Size: 219 1448 593 342 90 394 29 308 Cuts at: 4249 5384 6065 6256 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1448 1135 681 593 394 342 308 219 191 90 29 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6901 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6901 SmaI CCC'GGG Cuts at: 1872 4228 1872 Size: 2356 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2705 2924 5005 5906 6168 820 Size: 197 926 762 219 2081 901 262 1553 Fragments arranged by size: 2081 1553 926 901 762 262 219 197 SnaBI TAC'GTA Cuts at: 493 4692 493 Size: 4199 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6901 SphI G_CATG'C Cuts at: 1132 4259 1132 Size: 3127 3774 SspI AAT'ATT Cuts at: 5 52 3029 4825 5 Size: 47 2977 1796 2081 Fragments arranged by size: 2977 2081 1796 47 StuI AGG'CCT Cuts at: 1517 3293 1517 Size: 1776 5125 StyI C'CwwG_G Cuts at: 513 1065 2004 2126 2635 3611 3973 4340 513 Size: 552 939 122 509 976 362 367 3074 Fragments arranged by size: 3074 976 939 552 509 367 362 122 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2439 3217 4928 4945 5098 5283 5728 Size: 1255 859 778 1711 17 153 185 445 Cuts at: 5728 325 Size: 1498 Fragments arranged by size: 1711 1498 1255 859 778 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5147 6859 96 Size: 274 80 33 51 1443 3170 1712 138 Fragments arranged by size: 3170 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2275 2307 2655 2687 2835 2867 6861 6893 2275 Size: 32 348 32 148 32 3994 32 2283 Fragments arranged by size: 3994 2283 348 148 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6901 XbaI T'CTAG_A Cuts at: 4237 4237 Size: 6901 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6901 XmaI C'CCGG_G Cuts at: 1870 4226 1870 Size: 2356 4545 XmnI GAAnn'nnTTC Cuts at: 2447 3329 5030 2447 Size: 882 1701 4318 Fragments arranged by size: 4318 1701 882 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3619 4709 276 Size: 53 83 186 936 2085 1090 2468 Fragments arranged by size: 2468 2085 1090 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BaeI BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI