(Circular) (Six-base) MAPSORT of: pFN21AE3624.seq Check: 9051 from: 1 to: 6520 pFN21AE3624 6520 bp With 182 enzymes: SgfI * December 28, 2011 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4330 278 Size: 53 83 186 936 2794 2468 Fragments arranged by size: 2794 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2592 3863 1937 Size: 655 1271 4594 Fragments arranged by size: 4594 1271 655 Acc65I G'GTAC_C Cuts at: 3841 3841 Size: 6520 AclI AA'CG_TT Cuts at: 1563 4647 5020 1563 Size: 3084 373 3063 Fragments arranged by size: 3084 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2048 3530 4583 5967 6235 1632 Size: 416 1482 1053 1384 268 1917 Fragments arranged by size: 1917 1482 1384 1053 416 268 AfeI AGC'GCT Cuts at: 2033 2033 Size: 6520 AflII C'TTAA_G Cuts at: 820 1017 3586 820 Size: 197 2569 3754 Fragments arranged by size: 3754 2569 197 AflIII A'CryG_T Cuts at: 1203 2826 3275 5419 6396 1203 Size: 1623 449 2144 977 1327 Fragments arranged by size: 2144 1623 1327 977 449 AgeI A'CCGG_T Cuts at: 5351 5351 Size: 6520 AhdI GACnn_n'nnGTC Cuts at: 5249 5249 Size: 6520 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6520 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2531 1184 Size: 1347 5173 AlwNI CAG_nnn'CTG Cuts at: 2422 2800 2857 4263 5835 2422 Size: 378 57 1406 1572 3107 Fragments arranged by size: 3107 1572 1406 378 57 ApaI G_GGCC'C Cuts at: 2745 2745 Size: 6520 ApaLI G'TGCA_C Cuts at: 4577 5733 4577 Size: 1156 5364 ApoI r'AATT_y Cuts at: 1493 2779 3593 3829 4055 4207 6316 1493 Size: 1286 814 236 226 152 2109 1697 Fragments arranged by size: 2109 1697 1286 814 236 226 152 AseI AT'TA_AT Cuts at: 160 1032 3368 5074 160 Size: 872 2336 1706 1606 Fragments arranged by size: 2336 1706 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6520 AvaI C'yCGr_G Cuts at: 1870 1943 2292 2445 2761 2868 3845 1870 Size: 73 349 153 316 107 977 4545 Fragments arranged by size: 4545 977 349 316 153 107 73 BamHI G'GATC_C Cuts at: 3531 3850 4217 3531 Size: 319 367 5834 Fragments arranged by size: 5834 367 319 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2087 2635 3841 5296 618 Size: 325 217 627 300 548 1206 1455 1842 Fragments arranged by size: 1842 1455 1206 627 548 325 300 217 BanII G_rGCy'C Cuts at: 1391 2541 2559 2745 3011 3111 3269 3839 1391 Size: 1150 18 186 266 100 158 570 4072 Fragments arranged by size: 4072 1150 570 266 186 158 100 18 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2801 3231 3298 928 Size: 971 902 430 67 4150 Fragments arranged by size: 4150 971 902 430 67 BbvCI CC'TCA_GC Cuts at: 2324 2369 2324 Size: 45 6475 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4711 4745 1903 Size: 34 2774 34 3678 Fragments arranged by size: 3678 2774 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4412 5622 4412 Size: 1210 5310 BclI T'GATC_A Cuts at: 1548 1548 Size: 6520 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2120 5131 136 Size: 107 122 71 631 605 448 3011 1525 Fragments arranged by size: 3011 1525 631 605 448 122 107 71 BglII A'GATC_T Cuts at: 3492 6515 3492 Size: 3023 3497 BlpI GC'TnA_GC Cuts at: 3342 3937 3342 Size: 595 5925 Bme1580I G_kGCm'C Cuts at: 1790 2640 2745 2794 4581 5737 1790 Size: 850 105 49 1787 1156 2573 Fragments arranged by size: 2573 1787 1156 850 105 49 BmgBI CAC'GTC Cuts at: 1318 3276 1318 Size: 1958 4562 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2271 4251 5209 Size: 416 53 83 399 24 854 1980 958 Cuts at: 5209 442 Size: 1753 Fragments arranged by size: 1980 1753 958 854 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6520 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2883 2883 Size: 6520 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2156 2440 2621 2737 2876 3715 3736 5180 Size: 917 284 181 116 139 839 21 1444 Cuts at: 5180 1239 Size: 2579 Fragments arranged by size: 2579 1444 917 839 284 181 139 116 21 Bpu10I CC'TnA_GC Cuts at: 2324 2369 2858 3078 2324 Size: 45 489 220 5766 Fragments arranged by size: 5766 489 220 45 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4645 5510 5808 4645 Size: 865 298 5357 Fragments arranged by size: 5357 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2242 3133 3570 5183 882 Size: 617 743 891 437 1613 2219 Fragments arranged by size: 2219 1613 891 743 617 437 BsaAI yAC'GTr Cuts at: 493 1204 2829 4311 493 Size: 711 1625 1482 2702 Fragments arranged by size: 2702 1625 1482 711 BsaBI GATnn'nnATC Cuts at: 6514 6514 Size: 6520 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3774 4327 4709 5344 Size: 53 83 186 936 2241 553 382 635 Cuts at: 5344 275 Size: 1451 Fragments arranged by size: 2241 1451 936 635 553 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2463 2481 3653 4953 5351 5625 5772 6183 2463 Size: 18 1172 1300 398 274 147 411 2800 Fragments arranged by size: 2800 1300 1172 411 398 274 147 18 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6295 Fragments arranged by size: 6295 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2008 2200 1186 Size: 822 192 5506 Fragments arranged by size: 5506 822 192 BseYI C'CCAG_C Cuts at: 2010 2744 5723 6219 2010 Size: 734 2979 496 2311 Fragments arranged by size: 2979 2311 734 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2527 2900 1163 Size: 573 791 373 4783 Fragments arranged by size: 4783 791 573 373 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3970 4731 5759 1540 Size: 275 186 1969 761 1028 2301 Fragments arranged by size: 2301 1969 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2541 3269 3504 4581 4666 5737 2541 Size: 728 235 1077 85 1071 3324 Fragments arranged by size: 3324 1077 1071 728 235 85 BsmI GAATG_Cn' Cuts at: 4031 4124 4031 Size: 93 6427 BsmBI CGTCTCn'nnnn_ Cuts at: 2711 2763 2711 Size: 52 6468 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2541 2559 2640 2745 2794 3011 3111 Size: 399 751 18 81 105 49 217 100 Cuts at: 3111 3269 3504 3839 4581 4666 5737 1391 Size: 158 235 335 742 85 1071 2174 Fragments arranged by size: 2174 1071 751 742 399 335 235 217 158 105 100 85 81 49 18 BspEI T'CCGG_A Cuts at: 2463 3653 6183 2463 Size: 1190 2530 2800 Fragments arranged by size: 2800 2530 1190 BspHI T'CATG_A Cuts at: 2835 4409 6139 2835 Size: 1574 1730 3216 Fragments arranged by size: 3216 1730 1574 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2385 2922 3789 3875 844 Size: 1541 537 867 86 3489 Fragments arranged by size: 3489 1541 867 537 86 BsrBI CCG'CTC Cuts at: 1394 1823 2305 2751 4407 1394 Size: 429 482 446 1656 3507 Fragments arranged by size: 3507 1656 482 446 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2935 3336 5015 5189 65 Size: 1183 1687 401 1679 174 1396 Fragments arranged by size: 1687 1679 1396 1183 401 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2161 5164 5351 1700 Size: 110 351 3003 187 2869 Fragments arranged by size: 3003 2869 351 187 110 BsrGI T'GTAC_A Cuts at: 96 3278 96 Size: 3182 3338 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6520 BssSI C'ACGA_G Cuts at: 2658 2945 4580 5592 2658 Size: 287 1635 1012 3586 Fragments arranged by size: 3586 1635 1012 287 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6520 BstBI TT'CG_AA Cuts at: 2812 5324 2812 Size: 2512 4008 BstEII G'GTnAC_C Cuts at: 1184 2404 1184 Size: 1220 5300 BstXI CCAn_nnnn'nTGG Cuts at: 2500 3001 2500 Size: 501 6019 BstYI r'GATC_y Cuts at: 1972 3030 3241 3492 3531 3850 4217 4602 4619 Size: 1058 211 251 39 319 367 385 17 Cuts at: 4619 6071 6157 6169 6515 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1058 385 367 346 319 251 211 86 39 17 12 Bsu36I CC'TnA_GG Cuts at: 3357 3357 Size: 6520 BtgI C'CryG_G Cuts at: 513 1065 2920 3663 513 Size: 552 1855 743 3370 Fragments arranged by size: 3370 1855 743 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5781 Fragments arranged by size: 5781 683 56 BtsI GCAGTG_nn' Cuts at: 2323 2629 2858 3329 4036 4830 4850 2323 Size: 306 229 471 707 794 20 3993 Fragments arranged by size: 3993 794 707 471 306 229 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6520 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6485 DraI TTT'AAA Cuts at: 3824 3824 Size: 6520 DraIII CAC_nnn'GTG Cuts at: 2712 2712 Size: 6520 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3734 5527 809 Size: 507 2418 1793 1802 Fragments arranged by size: 2418 1802 1793 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2698 3670 3798 Size: 54 1425 50 275 18 868 972 128 Cuts at: 3798 3967 4856 6485 8 Size: 169 889 1629 43 Fragments arranged by size: 1629 1425 972 889 868 275 169 128 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3967 1537 Size: 275 2155 4090 Fragments arranged by size: 4090 2155 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2761 2946 3392 3791 4450 1353 Size: 1408 185 446 399 659 3423 Fragments arranged by size: 3423 1408 659 446 399 185 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5107 5481 5627 387 Size: 1064 138 3518 374 146 1280 Fragments arranged by size: 3518 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2539 3267 2539 Size: 728 5792 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2048 2156 2440 2621 2737 2876 3530 Size: 393 416 108 284 181 116 139 654 Cuts at: 3530 3715 3736 4583 5180 5967 6235 1239 Size: 185 21 847 597 787 268 1524 Fragments arranged by size: 1524 847 787 654 597 416 393 284 268 185 181 139 116 108 21 EcoO109I rG'GnC_Cy Cuts at: 2647 2734 2911 3322 2647 Size: 87 177 411 5845 Fragments arranged by size: 5845 411 177 87 EcoRI G'AATT_C Cuts at: 2779 3829 4207 2779 Size: 1050 378 5092 Fragments arranged by size: 5092 1050 378 FspI TGC'GCA Cuts at: 3764 5026 3764 Size: 1262 5258 FspAI rTGC'GCAy Cuts at: 3764 3764 Size: 6520 HaeII r_GCGC'y Cuts at: 1678 2035 5667 1678 Size: 357 3632 2531 Fragments arranged by size: 3632 2531 357 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2241 2273 2417 2449 3196 Size: 32 135 32 669 32 144 32 747 Cuts at: 3196 3226 3228 3258 5161 5193 5235 5267 1373 Size: 30 2 30 1903 32 42 32 2626 Fragments arranged by size: 2626 1903 747 669 144 135 42 32 32 32 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 669 1657 1938 2593 3864 4110 669 Size: 988 281 655 1271 246 3079 Fragments arranged by size: 3079 1271 988 655 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6520 HpaI GTT'AAC Cuts at: 4110 4110 Size: 6520 KpnI G_GTAC'C Cuts at: 3845 3845 Size: 6520 MfeI C'AATT_G Cuts at: 4119 4119 Size: 6520 MluI A'CGCG_T Cuts at: 6396 6396 Size: 6520 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3174 5634 5818 1305 Size: 1869 2460 184 2007 Fragments arranged by size: 2460 2007 1869 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3672 3800 10 Size: 54 1425 343 1840 128 2730 Fragments arranged by size: 2730 1840 1425 343 128 54 MslI CAynn'nnrTG Cuts at: 518 1166 3743 4478 4837 4996 518 Size: 648 2577 735 359 159 2042 Fragments arranged by size: 2577 2042 735 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2328 2337 2574 3936 4613 5761 6006 1606 Size: 722 9 237 1362 677 1148 245 2120 Fragments arranged by size: 2120 1362 1148 722 677 245 237 9 NcoI C'CATG_G Cuts at: 513 1065 3663 513 Size: 552 2598 3370 Fragments arranged by size: 3370 2598 552 NdeI CA'TA_TG Cuts at: 387 2978 387 Size: 2591 3929 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6520 NotI GC'GGCC_GC Cuts at: 3967 3967 Size: 6520 NspI r_CATG'y Cuts at: 1132 1229 2908 3432 3878 5423 1132 Size: 97 1679 524 446 1545 2229 Fragments arranged by size: 2229 1679 1545 524 446 97 PasI CC'CwG_GG Cuts at: 1348 2738 2794 1348 Size: 1390 56 5074 Fragments arranged by size: 5074 1390 56 PciI A'CATG_T Cuts at: 5419 5419 Size: 6520 PflMI CCAn_nnn'nTGG Cuts at: 1108 2422 2800 1108 Size: 1314 378 4828 Fragments arranged by size: 4828 1314 378 PfoI T'CCnGG_A Cuts at: 2217 3527 2217 Size: 1310 5210 PmeI GTTT'AAAC Cuts at: 3824 3824 Size: 6520 PmlI CAC'GTG Cuts at: 2829 2829 Size: 6520 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4589 4621 6102 6134 4589 Size: 32 1481 32 4975 Fragments arranged by size: 4975 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2911 3322 2911 Size: 411 6109 PshAI GACnn'nnGTC Cuts at: 1613 3145 1613 Size: 1532 4988 PsiI TTA'TAA Cuts at: 4090 4090 Size: 6520 PspOMI G'GGCC_C Cuts at: 2741 2741 Size: 6520 PstI C_TGCA'G Cuts at: 830 2553 2853 3872 830 Size: 1723 300 1019 3478 Fragments arranged by size: 3478 1723 1019 300 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6520 PvuII CAG'CTG Cuts at: 2574 2574 Size: 6520 SacI G_AGCT'C Cuts at: 2541 3269 2541 Size: 728 5792 SalI G'TCGA_C Cuts at: 1936 2591 3862 1936 Size: 655 1271 4594 Fragments arranged by size: 4594 1271 655 SapI GCTCTTCn'nnn_ Cuts at: 3392 3791 3392 Size: 399 6121 SbfI CC_TGCA'GG Cuts at: 2553 3872 2553 Size: 1319 5201 ScaI AGT'ACT Cuts at: 4768 6480 4768 Size: 1712 4808 SexAI A'CCwGG_T Cuts at: 2586 2586 Size: 6520 SfcI C'TryA_G Cuts at: 826 1045 2549 2849 3868 5003 5684 5875 826 Size: 219 1504 300 1019 1135 681 191 1471 Fragments arranged by size: 1504 1471 1135 1019 681 300 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6520 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6520 SmaI CCC'GGG Cuts at: 1872 2294 2447 2763 3847 1872 Size: 422 153 316 1084 4545 Fragments arranged by size: 4545 1084 422 316 153 SmlI C'TyrA_G Cuts at: 820 1017 1943 3586 4624 5525 5787 820 Size: 197 926 1643 1038 901 262 1553 Fragments arranged by size: 1643 1553 1038 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4311 493 Size: 3818 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6520 SphI G_CATG'C Cuts at: 1132 3878 1132 Size: 2746 3774 SspI AAT'ATT Cuts at: 5 52 4444 5 Size: 47 4392 2081 Fragments arranged by size: 4392 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6520 StyI C'CwwG_G Cuts at: 513 1065 2818 3663 3959 513 Size: 552 1753 845 296 3074 Fragments arranged by size: 3074 1753 845 552 296 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3656 4547 4564 4717 4902 5347 325 Size: 1255 2076 891 17 153 185 445 1498 Fragments arranged by size: 2076 1498 1255 891 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3278 4766 6478 Size: 274 80 33 51 1443 1301 1488 1712 Cuts at: 6478 96 Size: 138 Fragments arranged by size: 1712 1488 1443 1301 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6480 6512 6480 Size: 32 6488 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6520 XbaI T'CTAG_A Cuts at: 3214 3856 3214 Size: 642 5878 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2801 2801 Size: 6520 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6520 XmaI C'CCGG_G Cuts at: 1870 2292 2445 2761 3845 1870 Size: 422 153 316 1084 4545 Fragments arranged by size: 4545 1084 422 316 153 XmnI GAAnn'nnTTC Cuts at: 3515 4649 3515 Size: 1134 5386 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4328 276 Size: 53 83 186 936 2794 2468 Fragments arranged by size: 2794 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I EcoNI EcoRV FalI FseI KasI NaeI NarI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI