(Linear) (Six-base) MAPSORT of: KIEE3624.seq Check: 6738 from: 1 to: 1815 KIEE3624.orf With 182 enzymes: SgfI * December 27, 2011 16:36 .. AccI GT'mk_AC Cuts at: 0 587 1815 Size: 587 1228 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 43 1525 1815 Size: 43 1482 290 Fragments arranged by size: 1482 290 43 AfeI AGC'GCT Cuts at: 0 28 1815 Size: 28 1787 AflII C'TTAA_G Cuts at: 0 1581 1815 Size: 1581 234 AflIII A'CryG_T Cuts at: 0 821 1270 1815 Size: 821 449 545 Fragments arranged by size: 821 545 449 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 526 1815 Size: 526 1289 AlwNI CAG_nnn'CTG Cuts at: 0 417 795 852 1815 Size: 417 378 57 963 Fragments arranged by size: 963 417 378 57 ApaI G_GGCC'C Cuts at: 0 740 1815 Size: 740 1075 ApoI r'AATT_y Cuts at: 0 774 1588 1815 Size: 774 814 227 Fragments arranged by size: 814 774 227 AseI AT'TA_AT Cuts at: 0 1363 1815 Size: 1363 452 AvaI C'yCGr_G Cuts at: 0 287 440 756 863 1815 Size: 287 153 316 107 952 Fragments arranged by size: 952 316 287 153 107 BamHI G'GATC_C Cuts at: 0 1526 1815 Size: 1526 289 BanI G'GyrC_C Cuts at: 0 82 630 1815 Size: 82 548 1185 Fragments arranged by size: 1185 548 82 BanII G_rGCy'C Cuts at: 0 536 554 740 1006 1106 1264 1815 Size: 536 18 186 266 100 158 551 Fragments arranged by size: 551 536 266 186 158 100 18 BbsI GAAGACnn'nnnn_ Cuts at: 0 796 1226 1293 1815 Size: 796 430 67 522 Fragments arranged by size: 796 522 430 67 BbvCI CC'TCA_GC Cuts at: 0 319 364 1815 Size: 319 45 1451 Fragments arranged by size: 1451 319 45 BglI GCCn_nnn'nGGC Cuts at: 0 115 1815 Size: 115 1700 BglII A'GATC_T Cuts at: 0 1487 1815 Size: 1487 328 BlpI GC'TnA_GC Cuts at: 0 1337 1815 Size: 1337 478 Bme1580I G_kGCm'C Cuts at: 0 635 740 789 1815 Size: 635 105 49 1026 Fragments arranged by size: 1026 635 105 49 BmgBI CAC'GTC Cuts at: 0 1271 1815 Size: 1271 544 BmrI ACTGGGnnnn_n' Cuts at: 0 266 1815 Size: 266 1549 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 878 1815 Size: 878 937 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 151 435 616 732 871 1710 1731 1815 Size: 151 284 181 116 139 839 21 84 Fragments arranged by size: 839 284 181 151 139 116 84 21 Bpu10I CC'TnA_GC Cuts at: 0 319 364 853 1073 1815 Size: 319 45 489 220 742 Fragments arranged by size: 742 489 319 220 45 BsaI GGTCTCn'nnnn_ Cuts at: 0 237 1128 1565 1815 Size: 237 891 437 250 Fragments arranged by size: 891 437 250 237 BsaAI yAC'GTr Cuts at: 0 824 1815 Size: 824 991 BsaHI Gr'CG_yC Cuts at: 0 1769 1815 Size: 1769 46 BsaWI w'CCGG_w Cuts at: 0 458 476 1648 1815 Size: 458 18 1172 167 Fragments arranged by size: 1172 458 167 18 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 3 195 1815 Size: 3 192 1620 Fragments arranged by size: 1620 192 3 BseYI C'CCAG_C Cuts at: 0 5 739 1815 Size: 5 734 1076 Fragments arranged by size: 1076 734 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 522 895 1815 Size: 522 373 920 Fragments arranged by size: 920 522 373 BsiHKAI G_wGCw'C Cuts at: 0 536 1264 1499 1815 Size: 536 728 235 316 Fragments arranged by size: 728 536 316 235 BsmBI CGTCTCn'nnnn_ Cuts at: 0 706 758 1815 Size: 706 52 1057 Fragments arranged by size: 1057 706 52 Bsp1286I G_dGCh'C Cuts at: 0 536 554 635 740 789 1006 1106 1264 Size: 536 18 81 105 49 217 100 158 Cuts at: 1264 1499 1815 Size: 235 316 Fragments arranged by size: 536 316 235 217 158 105 100 81 49 18 BspEI T'CCGG_A Cuts at: 0 458 1648 1815 Size: 458 1190 167 Fragments arranged by size: 1190 458 167 BspHI T'CATG_A Cuts at: 0 830 1815 Size: 830 985 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 380 917 1784 1815 Size: 380 537 867 31 Fragments arranged by size: 867 537 380 31 BsrBI CCG'CTC Cuts at: 0 300 746 1815 Size: 300 446 1069 Fragments arranged by size: 1069 446 300 BsrDI GCAATG_nn' Cuts at: 0 930 1331 1815 Size: 930 401 484 Fragments arranged by size: 930 484 401 BsrFI r'CCGG_y Cuts at: 0 156 1815 Size: 156 1659 BsrGI T'GTAC_A Cuts at: 0 1273 1815 Size: 1273 542 BssSI C'ACGA_G Cuts at: 0 653 940 1815 Size: 653 287 875 Fragments arranged by size: 875 653 287 BstBI TT'CG_AA Cuts at: 0 807 1815 Size: 807 1008 BstEII G'GTnAC_C Cuts at: 0 399 1815 Size: 399 1416 BstXI CCAn_nnnn'nTGG Cuts at: 0 495 996 1815 Size: 495 501 819 Fragments arranged by size: 819 501 495 BstYI r'GATC_y Cuts at: 0 1025 1236 1487 1526 1815 Size: 1025 211 251 39 289 Fragments arranged by size: 1025 289 251 211 39 Bsu36I CC'TnA_GG Cuts at: 0 1352 1815 Size: 1352 463 BtgI C'CryG_G Cuts at: 0 915 1658 1815 Size: 915 743 157 Fragments arranged by size: 915 743 157 BtsI GCAGTG_nn' Cuts at: 0 318 624 853 1324 1815 Size: 318 306 229 471 491 Fragments arranged by size: 491 471 318 306 229 DraIII CAC_nnn'GTG Cuts at: 0 707 1815 Size: 707 1108 DrdI GACnn_nn'nnGTC Cuts at: 0 1729 1815 Size: 1729 86 EaeI y'GGCC_r Cuts at: 0 693 1665 1793 1815 Size: 693 972 128 22 Fragments arranged by size: 972 693 128 22 EarI CTCTTCn'nnn_ Cuts at: 0 756 941 1387 1786 1815 Size: 756 185 446 399 29 Fragments arranged by size: 756 446 399 185 29 EcoICRI GAG'CTC Cuts at: 0 534 1262 1815 Size: 534 728 553 Fragments arranged by size: 728 553 534 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 43 151 435 616 732 871 1525 1710 Size: 43 108 284 181 116 139 654 185 Cuts at: 1710 1731 1815 Size: 21 84 Fragments arranged by size: 654 284 185 181 139 116 108 84 43 21 EcoO109I rG'GnC_Cy Cuts at: 0 642 729 906 1317 1815 Size: 642 87 177 411 498 Fragments arranged by size: 642 498 411 177 87 EcoRI G'AATT_C Cuts at: 0 774 1815 Size: 774 1041 FspI TGC'GCA Cuts at: 0 1759 1815 Size: 1759 56 FspAI rTGC'GCAy Cuts at: 0 1759 1815 Size: 1759 56 HaeII r_GCGC'y Cuts at: 0 30 1815 Size: 30 1785 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 236 268 412 444 1191 1221 1223 1253 Size: 236 32 144 32 747 30 2 30 Cuts at: 1253 1815 Size: 562 Fragments arranged by size: 747 562 236 144 32 32 30 30 2 HincII GTy'rAC Cuts at: 0 588 1815 Size: 588 1227 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1169 1815 Size: 1169 646 MscI TGG'CCA Cuts at: 0 1667 1795 1815 Size: 1667 128 20 Fragments arranged by size: 1667 128 20 MslI CAynn'nnrTG Cuts at: 0 1738 1815 Size: 1738 77 MspA1I CmG'CkG Cuts at: 0 323 332 569 1815 Size: 323 9 237 1246 Fragments arranged by size: 1246 323 237 9 NcoI C'CATG_G Cuts at: 0 1658 1815 Size: 1658 157 NdeI CA'TA_TG Cuts at: 0 973 1815 Size: 973 842 NspI r_CATG'y Cuts at: 0 903 1427 1815 Size: 903 524 388 Fragments arranged by size: 903 524 388 PasI CC'CwG_GG Cuts at: 0 733 789 1815 Size: 733 56 1026 Fragments arranged by size: 1026 733 56 PflMI CCAn_nnn'nTGG Cuts at: 0 417 795 1815 Size: 417 378 1020 Fragments arranged by size: 1020 417 378 PfoI T'CCnGG_A Cuts at: 0 212 1522 1815 Size: 212 1310 293 Fragments arranged by size: 1310 293 212 PmlI CAC'GTG Cuts at: 0 824 1815 Size: 824 991 PpuMI rG'GwC_Cy Cuts at: 0 906 1317 1815 Size: 906 411 498 Fragments arranged by size: 906 498 411 PshAI GACnn'nnGTC Cuts at: 0 1140 1815 Size: 1140 675 PspOMI G'GGCC_C Cuts at: 0 736 1815 Size: 736 1079 PstI C_TGCA'G Cuts at: 0 548 848 1815 Size: 548 300 967 Fragments arranged by size: 967 548 300 PvuII CAG'CTG Cuts at: 0 569 1815 Size: 569 1246 SacI G_AGCT'C Cuts at: 0 536 1264 1815 Size: 536 728 551 Fragments arranged by size: 728 551 536 SalI G'TCGA_C Cuts at: 0 586 1815 Size: 586 1229 SapI GCTCTTCn'nnn_ Cuts at: 0 1387 1786 1815 Size: 1387 399 29 Fragments arranged by size: 1387 399 29 SbfI CC_TGCA'GG Cuts at: 0 548 1815 Size: 548 1267 SexAI A'CCwGG_T Cuts at: 0 581 1815 Size: 581 1234 SfcI C'TryA_G Cuts at: 0 544 844 1815 Size: 544 300 971 Fragments arranged by size: 971 544 300 SmaI CCC'GGG Cuts at: 0 289 442 758 1815 Size: 289 153 316 1057 Fragments arranged by size: 1057 316 289 153 SmlI C'TyrA_G Cuts at: 0 1581 1815 Size: 1581 234 StyI C'CwwG_G Cuts at: 0 813 1658 1815 Size: 813 845 157 Fragments arranged by size: 845 813 157 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1651 1815 Size: 1651 164 TatI w'GTAC_w Cuts at: 0 1273 1815 Size: 1273 542 XbaI T'CTAG_A Cuts at: 0 1209 1815 Size: 1209 606 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 796 1815 Size: 796 1019 XmaI C'CCGG_G Cuts at: 0 287 440 756 1815 Size: 287 153 316 1059 Fragments arranged by size: 1059 316 287 153 XmnI GAAnn'nnTTC Cuts at: 0 1510 1815 Size: 1510 305 Enzymes that do cut: AccI AcuI AfeI AflII AflIII AlfI AlwNI ApaI ApoI AseI AvaI BamHI BanI BanII BbsI BbvCI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaI BsaAI BsaHI BsaWI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstBI BstEII BstXI BstYI Bsu36I BtgI BtsI DraIII DrdI EaeI EarI EcoICRI Eco57MI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII MmeI MscI MslI MspA1I NcoI NdeI NspI PasI PflMI PfoI PmlI PpuMI PshAI PspOMI PstI PvuII SacI SalI SapI SbfI SexAI SfcI SmaI SmlI StyI TaqII TatI XbaI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AgeI AhdI AleI AloI ApaLI AscI AsiSI AvrII BaeI BbeI BcgI BciVI BclI BfrBI BmtI BpuEI BsaBI BsaXI BsiEI BsiWI BsmI BssHII BstAPI BstZ17I BtgZI ClaI CspCI DraI EagI EciI EcoNI EcoRV FalI FseI HindIII HpaI KasI KpnI MfeI MluI NaeI NarI NgoMIV NheI NotI NruI NsiI PacI PciI PmeI PpiI PsiI PspXI PsrI PvuI RsrII SacII SanDI ScaI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI TstI Tth111I XhoI ZraI