################################## # NO CUT ENZYMES ( 132 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BanI BbeI BbrPI BcgI BciVI BfrI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BseAI BsiEI BsiWI BspDI BspEI BspHI BspLU11I BsrBI BsrFI BssHII BssSI Bst1107I BstBI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoRI EcoRV EcoT22I FseI FspI Hin1I HindIII HpaI KasI KpnI Ksp632I KspI MamI MfeI MluI MroI MslI MunI NaeI NarI NcoI NdeI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI TaqI Tth111I TthHB8I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AfaI 990 GTAC GT^AC AlwI 182 GGATC GGATC(4/5) AseI 655 ATTAAT AT^TAAT AsnI 655 ATTAAT AT^TAAT AvaI 1112 CCCGGG C^[CT]CG[GA]G BbsI 1313 GTCTTC GAAGAC(2/6) BbvCI 1095 CCTCAGC CCTCAGC(-5/-2) BclI 764 TGATCA T^GATCA BpuAI 1313 GTCTTC GAAGAC(2/6) BsaWI 179 ACCGGA [AT]^CCGG[AT] BsmBI 808 GAGACG CGTCTC(1/5) BsoBI 1112 CCCGGG C^[CT]CG[GA]G Bsp1407I 989 TGTACA T^GTACA BspMI 1262 GCAGGT ACCTGC(4/8) BsrDI 541 GCAATG GCAATG(2/0) BsrGI 989 TGTACA T^GTACA BstEII 720 GGTCACC G^GT[ACGT]ACC BstF5I 198 GGATG GGATG(2/0) BstPI 720 GGTCACC G^GT[ACGT]ACC BstYI 182 GGATCT [GA]^GATC[CT] DraI 757 TTTAAA TTT^AAA EcoNI 78 CCTCAACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 720 GGTCACC G^GT[ACGT]ACC FbaI 764 TGATCA T^GATCA FokI 198 GGATG GGATG(9/13) HaeII 844 AGCGCC [GA]GCGC^[CT] HincII 115 GTTGAC GT[CT]^[GA]AC HindII 115 GTTGAC GT[CT]^[GA]AC MaeII 1289 ACGT A^CGT MflI 182 GGATCT [GA]^GATC[CT] NheI 204 GCTAGC G^CTAGC NspI 1278 GCATGT [GA]CATG^[CT] PflMI 189 CCAGCAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 230 GAGTC GAGTC(4/5) PshBI 655 ATTAAT AT^TAAT RsaI 990 GTAC GT^AC SexAI 83 ACCAGGT A^CC[AT]GGT SmaI 1112 CCCGGG CCC^GGG SspBI 989 TGTACA T^GTACA Van91I 189 CCAGCAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 182 GGATCT [GA]^GATC[CT] XmaCI 1112 CCCGGG C^CCGGG XmaI 1112 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AccII 27 CGCG CG^CG 1268 CGCG AlwNI 592 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG 1080 CAGCAGCTG BalI 686 TGGCCA TGG^CCA 1206 TGGCCA BcnI 1112 CCCGG CC^[GC]GG 1113 CCGGG BfaI 205 CTAG C^TAG 275 CTAG BsgI 90 GTGCAG GTGCAG(16/14) 679 GTGCAG BsmAI 386 GAGAC GTCTC(1/5) 808 GAGAC BsmI 567 GCATTC GAATGC(1/-1) 817 GCATTC BstUI 27 CGCG CG^CG 1268 CGCG CfoI 845 GCGC GCG^C 1232 GCGC DpnII 183 GATC ^GATC 765 GATC DraII 305 AGGCCCT [GA]G^G[ACGT]CC[CT] 1091 AGGGCCT DraIII 1210 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG 1212 CACTGTGTG EcoO109I 305 AGGCCCT [GA]G^G[ACGT]CC[CT] 1091 AGGGCCT FauI 576 GCGGG CCCGC(4/6) 785 GCGGG HgaI 25 GACGC GACGC(5/10) 810 GACGC HhaI 845 GCGC GCG^C 1232 GCGC HinP1I 845 GCGC G^CGC 1232 GCGC HphI 151 GGTGA GGTGA(8/7) 722 TCACC MaeI 205 CTAG C^TAG 275 CTAG MboI 183 GATC ^GATC 765 GATC MboII 239 TCTTC GAAGA(8/7) 1314 TCTTC MluNI 686 TGGCCA TGG^CCA 1206 TGGCCA MscI 686 TGGCCA TGG^CCA 1206 TGGCCA MvnI 27 CGCG CG^CG 1268 CGCG NciI 1112 CCCGG CC^[GC]GG 1113 CCGGG NdeII 183 GATC ^GATC 765 GATC NlaIV 246 GGAGCC GG[ACGT]^[ACGT]CC 493 GGGACC SacI 424 GAGCTC GAGCT^C 439 GAGCTC Sau3AI 183 GATC ^GATC 765 GATC Tsp509I 630 AATT ^AATT 654 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 52 enzymes) # ######################################### AciI AluI AspHI AvaII BanII BbvI BglI BmyI BsaJI BseRI BsiHKAI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstAPI BstNI Cac8I Cfr13I DdeI EaeI EcoRII EcoT14I Fnu4HI HaeIII HapII HinfI HpaII ItaI MaeIII MnlI MseI MspA1I MspI MvaI MwoI NlaIII PstI PvuII Sau96I ScrFI SfaNI SfcI StyI TfiI Tru9I TseI Tsp45I TspRI XcmI