(Circular) (Six-base) MAPSORT of: pFN21AE3660.seq Check: 4478 from: 1 to: 6040 pFN21AE3660 6040 bp With 182 enzymes: SgfI * December 28, 2011 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3850 278 Size: 53 83 186 936 2314 2468 Fragments arranged by size: 2468 2314 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3383 1937 Size: 1446 4594 Acc65I G'GTAC_C Cuts at: 3361 3361 Size: 6040 AclI AA'CG_TT Cuts at: 1563 4167 4540 1563 Size: 2604 373 3063 Fragments arranged by size: 3063 2604 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2496 2643 3077 3167 4103 5487 5755 1632 Size: 864 147 434 90 936 1384 268 1917 Fragments arranged by size: 1917 1384 936 864 434 268 147 90 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5843 AflIII A'CryG_T Cuts at: 1203 4939 5916 1203 Size: 3736 977 1327 Fragments arranged by size: 3736 1327 977 AgeI A'CCGG_T Cuts at: 4871 4871 Size: 6040 AhdI GACnn_n'nnGTC Cuts at: 4769 4769 Size: 6040 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6040 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6040 AlwNI CAG_nnn'CTG Cuts at: 2602 3090 3783 5355 2602 Size: 488 693 1572 3287 Fragments arranged by size: 3287 1572 693 488 ApaLI G'TGCA_C Cuts at: 4097 5253 4097 Size: 1156 4884 ApoI r'AATT_y Cuts at: 1493 3349 3575 3727 5836 1493 Size: 1856 226 152 2109 1697 Fragments arranged by size: 2109 1856 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 2661 4594 160 Size: 872 1629 1933 1606 Fragments arranged by size: 1933 1629 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6040 AvaI C'yCGr_G Cuts at: 1870 1943 3117 3365 1870 Size: 73 1174 248 4545 Fragments arranged by size: 4545 1174 248 73 BamHI G'GATC_C Cuts at: 3370 3737 3370 Size: 367 5673 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3361 4816 618 Size: 325 217 627 1574 1455 1842 Fragments arranged by size: 1842 1574 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2433 2448 2796 2992 3041 3359 1391 Size: 1042 15 348 196 49 318 4072 Fragments arranged by size: 4072 1042 348 318 196 49 15 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3311 928 Size: 971 1412 3657 Fragments arranged by size: 3657 1412 971 BbvCI CC'TCA_GC Cuts at: 3101 3101 Size: 6040 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4231 4265 1903 Size: 34 50 34 2210 34 3678 Fragments arranged by size: 3678 2210 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3932 5142 3932 Size: 1210 4830 BclI T'GATC_A Cuts at: 1548 2769 1548 Size: 1221 4819 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2318 2372 2987 Size: 107 122 71 631 605 646 54 615 Cuts at: 2987 4651 136 Size: 1664 1525 Fragments arranged by size: 1664 1525 646 631 615 605 122 107 71 54 BglII A'GATC_T Cuts at: 6035 6035 Size: 6040 BlpI GC'TnA_GC Cuts at: 3457 3457 Size: 6040 Bme1580I G_kGCm'C Cuts at: 1790 2368 4101 5257 1790 Size: 578 1733 1156 2573 Fragments arranged by size: 2573 1733 1156 578 BmgBI CAC'GTC Cuts at: 1318 3295 1318 Size: 1977 4063 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2114 2692 3771 Size: 416 53 83 399 24 697 578 1079 Cuts at: 3771 4729 442 Size: 958 1753 Fragments arranged by size: 1753 1079 958 697 578 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2213 1056 Size: 1157 4883 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2467 2467 Size: 6040 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4700 1239 Size: 3461 2579 Bpu10I CC'TnA_GC Cuts at: 3101 3101 Size: 6040 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4165 5030 5328 4165 Size: 865 298 4877 Fragments arranged by size: 4877 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4703 882 Size: 617 3204 2219 Fragments arranged by size: 3204 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3831 493 Size: 711 2627 2702 Fragments arranged by size: 2702 2627 711 BsaBI GATnn'nnATC Cuts at: 6034 6034 Size: 6040 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3847 4229 4864 275 Size: 53 83 186 936 2314 382 635 1451 Fragments arranged by size: 2314 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2184 4473 4871 5145 5292 5703 2184 Size: 2289 398 274 147 411 2521 Fragments arranged by size: 2521 2289 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2174 2204 1178 Size: 30 165 30 771 30 5014 Fragments arranged by size: 5014 771 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2440 2441 3194 1186 Size: 1254 1 753 4032 Fragments arranged by size: 4032 1254 753 1 BseYI C'CCAG_C Cuts at: 2193 3243 5243 5739 2193 Size: 1050 2000 496 2494 Fragments arranged by size: 2494 2000 1050 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2116 2705 1163 Size: 573 380 589 4498 Fragments arranged by size: 4498 589 573 380 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3490 4251 5279 1540 Size: 275 186 1489 761 1028 2301 Fragments arranged by size: 2301 1489 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2433 2448 2757 4101 4186 5257 2433 Size: 15 309 1344 85 1071 3216 Fragments arranged by size: 3216 1344 1071 309 85 15 BsmI GAATG_Cn' Cuts at: 2572 2822 3551 3644 2572 Size: 250 729 93 4968 Fragments arranged by size: 4968 729 250 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2807 2807 Size: 6040 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2368 2433 2448 2757 2796 2992 3041 Size: 399 578 65 15 309 39 196 49 Cuts at: 3041 3359 4101 4186 5257 1391 Size: 318 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 578 399 318 309 196 85 65 49 39 15 BspEI T'CCGG_A Cuts at: 5703 5703 Size: 6040 BspHI T'CATG_A Cuts at: 3929 5659 3929 Size: 1730 4310 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3258 3395 844 Size: 2414 137 3489 Fragments arranged by size: 3489 2414 137 BsrBI CCG'CTC Cuts at: 1394 1823 3927 1394 Size: 429 2104 3507 Fragments arranged by size: 3507 2104 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2553 4535 4709 65 Size: 1183 1305 1982 174 1396 Fragments arranged by size: 1982 1396 1305 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4684 4871 1700 Size: 110 2874 187 2869 Fragments arranged by size: 2874 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2994 96 Size: 2898 3142 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6040 BssSI C'ACGA_G Cuts at: 4100 5112 4100 Size: 1012 5028 BstAPI GCAn_nnn'nTGC Cuts at: 794 2578 2602 3081 3090 3171 794 Size: 1784 24 479 9 81 3663 Fragments arranged by size: 3663 1784 479 81 24 9 BstBI TT'CG_AA Cuts at: 4844 4844 Size: 6040 BstEII G'GTnAC_C Cuts at: 1184 2725 1184 Size: 1541 4499 BstYI r'GATC_y Cuts at: 1972 2187 3370 3737 4122 4139 5591 5677 5689 Size: 215 1183 367 385 17 1452 86 12 Cuts at: 5689 6035 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1183 385 367 346 215 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5488 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5301 Fragments arranged by size: 5301 683 56 BtsI GCAGTG_nn' Cuts at: 2358 2406 3556 4350 4370 2358 Size: 48 1150 794 20 4028 Fragments arranged by size: 4028 1150 794 48 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6040 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6005 DraI TTT'AAA Cuts at: 2764 3344 2764 Size: 580 5460 DraIII CAC_nnn'GTG Cuts at: 3220 3222 3220 Size: 2 6038 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5047 809 Size: 507 3731 1802 Fragments arranged by size: 3731 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2691 2784 3211 Size: 54 1425 50 275 18 861 93 427 Cuts at: 3211 3487 4376 6005 8 Size: 276 889 1629 43 Fragments arranged by size: 1629 1425 889 861 427 276 275 93 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3487 1537 Size: 275 1675 4090 Fragments arranged by size: 4090 1675 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3970 1353 Size: 2617 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4627 5001 5147 387 Size: 1064 138 3038 374 146 1280 Fragments arranged by size: 3038 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2431 2446 2431 Size: 15 6025 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2496 2643 3077 3167 4103 4700 5487 Size: 393 864 147 434 90 936 597 787 Cuts at: 5487 5755 1239 Size: 268 1524 Fragments arranged by size: 1524 936 864 787 597 434 393 268 147 90 EcoNI CCTnn'n_nnAGG Cuts at: 2087 2087 Size: 6040 EcoO109I rG'GnC_Cy Cuts at: 2311 3097 2311 Size: 786 5254 EcoRI G'AATT_C Cuts at: 3349 3727 3349 Size: 378 5662 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2905 2905 Size: 6040 FspI TGC'GCA Cuts at: 4546 4546 Size: 6040 HaeII r_GCGC'y Cuts at: 1678 2853 5187 1678 Size: 1175 2334 2531 Fragments arranged by size: 2531 2334 1175 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2094 2126 2659 2691 4681 Size: 32 135 32 522 32 533 32 1990 Cuts at: 4681 4713 4755 4787 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1990 533 522 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2122 3384 3630 669 Size: 988 281 184 1262 246 3079 Fragments arranged by size: 3079 1262 988 281 246 184 HindIII A'AGCT_T Cuts at: 748 748 Size: 6040 HpaI GTT'AAC Cuts at: 3630 3630 Size: 6040 KpnI G_GTAC'C Cuts at: 3365 3365 Size: 6040 MfeI C'AATT_G Cuts at: 3639 3639 Size: 6040 MluI A'CGCG_T Cuts at: 5916 5916 Size: 6040 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2472 2596 5154 5338 1305 Size: 1167 124 2558 184 2007 Fragments arranged by size: 2558 2007 1167 184 124 MscI TGG'CCA Cuts at: 10 64 1489 1832 2693 3213 10 Size: 54 1425 343 861 520 2837 Fragments arranged by size: 2837 1425 861 520 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3998 4357 4516 518 Size: 648 2832 359 159 2042 Fragments arranged by size: 2832 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2516 2790 3090 3105 3456 4133 5281 5526 Size: 910 274 300 15 351 677 1148 245 Cuts at: 5526 1606 Size: 2120 Fragments arranged by size: 2120 1148 910 677 351 300 274 245 15 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5488 NdeI CA'TA_TG Cuts at: 387 387 Size: 6040 NheI G'CTAG_C Cuts at: 1052 2209 1052 Size: 1157 4883 NotI GC'GGCC_GC Cuts at: 3487 3487 Size: 6040 NspI r_CATG'y Cuts at: 1132 1229 3287 3398 4943 1132 Size: 97 2058 111 1545 2229 Fragments arranged by size: 2229 2058 1545 111 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6040 PciI A'CATG_T Cuts at: 4939 4939 Size: 6040 PflMI CCAn_nnn'nTGG Cuts at: 1108 2200 1108 Size: 1092 4948 PmeI GTTT'AAAC Cuts at: 3344 3344 Size: 6040 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4109 4141 5622 5654 4109 Size: 32 1481 32 4495 Fragments arranged by size: 4495 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6040 PsiI TTA'TAA Cuts at: 3610 3610 Size: 6040 PstI C_TGCA'G Cuts at: 830 2139 3086 3129 3269 3392 830 Size: 1309 947 43 140 123 3478 Fragments arranged by size: 3478 1309 947 140 123 43 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6040 PvuII CAG'CTG Cuts at: 2516 3090 3105 2516 Size: 574 15 5451 Fragments arranged by size: 5451 574 15 SacI G_AGCT'C Cuts at: 2433 2448 2433 Size: 15 6025 SalI G'TCGA_C Cuts at: 1936 3382 1936 Size: 1446 4594 SbfI CC_TGCA'GG Cuts at: 3392 3392 Size: 6040 ScaI AGT'ACT Cuts at: 4288 6000 4288 Size: 1712 4328 SexAI A'CCwGG_T Cuts at: 2088 2088 Size: 6040 SfcI C'TryA_G Cuts at: 826 1045 2135 2827 3082 3125 3248 3265 3388 Size: 219 1090 692 255 43 123 17 123 Cuts at: 3388 4523 5204 5395 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 1090 692 681 255 219 191 123 123 43 17 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6040 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6040 SmaI CCC'GGG Cuts at: 1872 3119 3367 1872 Size: 1247 248 4545 Fragments arranged by size: 4545 1247 248 SmlI C'TyrA_G Cuts at: 820 1017 1943 4144 5045 5307 820 Size: 197 926 2201 901 262 1553 Fragments arranged by size: 2201 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3831 493 Size: 3338 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6040 SphI G_CATG'C Cuts at: 1132 3398 1132 Size: 2266 3774 SspI AAT'ATT Cuts at: 5 52 3964 5 Size: 47 3912 2081 Fragments arranged by size: 3912 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6040 StyI C'CwwG_G Cuts at: 513 1065 2439 2730 2923 3479 513 Size: 552 1374 291 193 556 3074 Fragments arranged by size: 3074 1374 556 552 291 193 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2711 4067 4084 4237 4422 4867 325 Size: 1255 1131 1356 17 153 185 445 1498 Fragments arranged by size: 1498 1356 1255 1131 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2994 4286 5998 Size: 274 80 33 51 1443 1017 1292 1712 Cuts at: 5998 96 Size: 138 Fragments arranged by size: 1712 1443 1292 1017 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3187 3219 6000 6032 3187 Size: 32 2781 32 3195 Fragments arranged by size: 3195 2781 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6040 XbaI T'CTAG_A Cuts at: 3376 3376 Size: 6040 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2201 2384 3163 2201 Size: 183 779 5078 Fragments arranged by size: 5078 779 183 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6040 XmaI C'CCGG_G Cuts at: 1870 3117 3365 1870 Size: 1247 248 4545 Fragments arranged by size: 4545 1247 248 XmnI GAAnn'nnTTC Cuts at: 4169 4169 Size: 6040 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3848 276 Size: 53 83 186 936 2314 2468 Fragments arranged by size: 2468 2314 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BstXI BstZ17I Bsu36I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI