(Linear) (Six-base) MAPSORT of: KIEE3660.seq Check: 8696 from: 1 to: 1335 KIEE3660.orf With 182 enzymes: SgfI * December 27, 2011 16:37 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 491 638 1072 1162 1335 Size: 491 147 434 90 173 Fragments arranged by size: 491 434 173 147 90 AlwNI CAG_nnn'CTG Cuts at: 0 597 1085 1335 Size: 597 488 250 Fragments arranged by size: 597 488 250 AseI AT'TA_AT Cuts at: 0 656 1335 Size: 656 679 AvaI C'yCGr_G Cuts at: 0 1112 1335 Size: 1112 223 BanII G_rGCy'C Cuts at: 0 428 443 791 987 1036 1335 Size: 428 15 348 196 49 299 Fragments arranged by size: 428 348 299 196 49 15 BbsI GAAGACnn'nnnn_ Cuts at: 0 1306 1335 Size: 1306 29 BbvCI CC'TCA_GC Cuts at: 0 1096 1335 Size: 1096 239 BclI T'GATC_A Cuts at: 0 764 1335 Size: 764 571 BglI GCCn_nnn'nGGC Cuts at: 0 313 367 982 1335 Size: 313 54 615 353 Fragments arranged by size: 615 353 313 54 Bme1580I G_kGCm'C Cuts at: 0 363 1335 Size: 363 972 BmgBI CAC'GTC Cuts at: 0 1290 1335 Size: 1290 45 BmrI ACTGGGnnnn_n' Cuts at: 0 109 687 1335 Size: 109 578 648 Fragments arranged by size: 648 578 109 BmtI G_CTAG'C Cuts at: 0 208 1335 Size: 208 1127 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 462 1335 Size: 462 873 Bpu10I CC'TnA_GC Cuts at: 0 1096 1335 Size: 1096 239 BsaWI w'CCGG_w Cuts at: 0 179 1335 Size: 179 1156 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 169 199 1335 Size: 169 30 1136 Fragments arranged by size: 1136 169 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 435 436 1189 1335 Size: 435 1 753 146 Fragments arranged by size: 753 435 146 1 BseYI C'CCAG_C Cuts at: 0 188 1238 1335 Size: 188 1050 97 Fragments arranged by size: 1050 188 97 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 111 700 1335 Size: 111 589 635 Fragments arranged by size: 635 589 111 BsiHKAI G_wGCw'C Cuts at: 0 428 443 752 1335 Size: 428 15 309 583 Fragments arranged by size: 583 428 309 15 BsmI GAATG_Cn' Cuts at: 0 567 817 1335 Size: 567 250 518 Fragments arranged by size: 567 518 250 BsmBI CGTCTCn'nnnn_ Cuts at: 0 802 1335 Size: 802 533 Bsp1286I G_dGCh'C Cuts at: 0 363 428 443 752 791 987 1036 1335 Size: 363 65 15 309 39 196 49 299 Fragments arranged by size: 363 309 299 196 65 49 39 15 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1253 1335 Size: 1253 82 BsrDI GCAATG_nn' Cuts at: 0 548 1335 Size: 548 787 BsrGI T'GTAC_A Cuts at: 0 989 1335 Size: 989 346 BstAPI GCAn_nnn'nTGC Cuts at: 0 573 597 1076 1085 1166 1335 Size: 573 24 479 9 81 169 Fragments arranged by size: 573 479 169 81 24 9 BstEII G'GTnAC_C Cuts at: 0 720 1335 Size: 720 615 BstYI r'GATC_y Cuts at: 0 182 1335 Size: 182 1153 BtsI GCAGTG_nn' Cuts at: 0 353 401 1335 Size: 353 48 934 Fragments arranged by size: 934 353 48 DraI TTT'AAA Cuts at: 0 759 1335 Size: 759 576 DraIII CAC_nnn'GTG Cuts at: 0 1215 1217 1335 Size: 1215 2 118 Fragments arranged by size: 1215 118 2 EaeI y'GGCC_r Cuts at: 0 686 779 1206 1335 Size: 686 93 427 129 Fragments arranged by size: 686 427 129 93 EcoICRI GAG'CTC Cuts at: 0 426 441 1335 Size: 426 15 894 Fragments arranged by size: 894 426 15 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 491 638 1072 1162 1335 Size: 491 147 434 90 173 Fragments arranged by size: 491 434 173 147 90 EcoNI CCTnn'n_nnAGG Cuts at: 0 82 1335 Size: 82 1253 EcoO109I rG'GnC_Cy Cuts at: 0 306 1092 1335 Size: 306 786 243 Fragments arranged by size: 786 306 243 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 900 1335 Size: 900 435 HaeII r_GCGC'y Cuts at: 0 848 1335 Size: 848 487 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 89 121 654 686 1335 Size: 89 32 533 32 649 Fragments arranged by size: 649 533 89 32 32 HincII GTy'rAC Cuts at: 0 117 1335 Size: 117 1218 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 467 591 1335 Size: 467 124 744 Fragments arranged by size: 744 467 124 MscI TGG'CCA Cuts at: 0 688 1208 1335 Size: 688 520 127 Fragments arranged by size: 688 520 127 MspA1I CmG'CkG Cuts at: 0 511 785 1085 1100 1335 Size: 511 274 300 15 235 Fragments arranged by size: 511 300 274 235 15 NheI G'CTAG_C Cuts at: 0 204 1335 Size: 204 1131 NspI r_CATG'y Cuts at: 0 1282 1335 Size: 1282 53 PflMI CCAn_nnn'nTGG Cuts at: 0 195 1335 Size: 195 1140 PstI C_TGCA'G Cuts at: 0 134 1081 1124 1264 1335 Size: 134 947 43 140 71 Fragments arranged by size: 947 140 134 71 43 PvuII CAG'CTG Cuts at: 0 511 1085 1100 1335 Size: 511 574 15 235 Fragments arranged by size: 574 511 235 15 SacI G_AGCT'C Cuts at: 0 428 443 1335 Size: 428 15 892 Fragments arranged by size: 892 428 15 SexAI A'CCwGG_T Cuts at: 0 83 1335 Size: 83 1252 SfcI C'TryA_G Cuts at: 0 130 822 1077 1120 1243 1260 1335 Size: 130 692 255 43 123 17 75 Fragments arranged by size: 692 255 130 123 75 43 17 SmaI CCC'GGG Cuts at: 0 1114 1335 Size: 1114 221 StyI C'CwwG_G Cuts at: 0 434 725 918 1335 Size: 434 291 193 417 Fragments arranged by size: 434 417 291 193 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 706 1335 Size: 706 629 TatI w'GTAC_w Cuts at: 0 989 1335 Size: 989 346 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1182 1214 1335 Size: 1182 32 121 Fragments arranged by size: 1182 121 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 196 379 1158 1335 Size: 196 183 779 177 Fragments arranged by size: 779 196 183 177 XmaI C'CCGG_G Cuts at: 0 1112 1335 Size: 1112 223 Enzymes that do cut: AcuI AlwNI AseI AvaI BanII BbsI BbvCI BclI BglI Bme1580I BmgBI BmrI BmtI BplI Bpu10I BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrDI BsrGI BstAPI BstEII BstYI BtsI DraI DraIII EaeI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HincII MmeI MscI MspA1I NheI NspI PflMI PstI PvuII SacI SexAI SfcI SmaI StyI TaqII TatI TstI XcmI XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI AsiSI AvrII BaeI BamHI BanI BbeI BcgI BciVI BfrBI BglII BlpI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsiEI BsiWI BspEI BspHI BsrBI BsrFI BssHII BssSI BstBI BstXI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DrdI EagI EarI EciI EcoRI EcoRV FseI FspI FspAI HindIII HpaI KasI KpnI MfeI MluI MslI NaeI NarI NcoI NdeI NgoMIV NotI NruI NsiI PacI PasI PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI XhoI XmnI ZraI