################################## # NO CUT ENZYMES ( 122 enzymes) # ################################## AatII AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AocI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AspI AvaI AviII AvrII BbrPI BcgI BciVI BclI BcnI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BseAI BsiWI BsmBI BsmI BsoBI BspDI BspEI BspHI BspLU11I BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII ClaI DraI DrdI Eam1105I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII HpaI MaeI MamI MfeI MluI MroI MseI MunI NaeI NciI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI SalI SbfI ScaI SexAI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI SwaI Tru9I Tsp509I Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### Aor51HI 575 AGCGCT AGC^GCT BalI 530 TGGCCA TGG^CCA BamHI 963 GGATCC G^GATCC BbeI 95 GGCGCC GGCGC^C BbsI 415 GTCTTC GAAGAC(2/6) BbvCI 957 GCTGAGG CCTCAGC(-5/-2) BglI 217 GCCTACATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 751 CTGGAG CTGGAG(16/14) BpuAI 415 GTCTTC GAAGAC(2/6) BseRI 664 CTCCTC GAGGAG(10/8) BsiEI 24 CGGCCG CG[GA][CT]^CG BsmAI 613 GTCTC GTCTC(1/5) Bsp1407I 314 TGTACA T^GTACA BsrDI 66 GCAATG GCAATG(2/0) BsrFI 26 GCCGGT [GA]^CCGG[CT] BsrGI 314 TGTACA T^GTACA BssHII 78 GCGCGC G^CGCGC BstAPI 66 GCAATGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 963 GGATCC [GA]^GATC[CT] Cfr10I 26 GCCGGT [GA]^CCGG[CT] CpoI 28 CGGTCCG CG^G[AT]CCG DraIII 184 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 24 CGGCCG C^GGCCG EclXI 24 CGGCCG C^GGCCG Eco47III 575 AGCGCT AGC^GCT Eco52I 24 CGGCCG C^GGCCG HgaI 239 GCGTC GACGC(5/10) HincII 879 GTTGAC GT[CT]^[GA]AC HindII 879 GTTGAC GT[CT]^[GA]AC KasI 95 GGCGCC G^GCGCC KspI 38 CCGCGG CCGC^GG MaeII 1082 ACGT A^CGT MflI 963 GGATCC [GA]^GATC[CT] MluNI 530 TGGCCA TGG^CCA MscI 530 TGGCCA TGG^CCA NarI 95 GGCGCC GG^CGCC NcoI 533 CCATGG C^CATGG NdeI 1230 CATATG CA^TATG PleI 56 GACTC GAGTC(4/5) PvuII 302 CAGCTG CAG^CTG RsrII 28 CGGTCCG CG^G[AT]CCG SacI 1177 GAGCTC GAGCT^C SacII 38 CCGCGG CCGC^GG SfiI 216 GGCCTACATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 95 GGCGCC GGC^GCC SspBI 314 TGTACA T^GTACA StuI 215 AGGCCT AGG^CCT TfiI 707 GAATC G^A[AT]TC Tsp45I 278 GTGAC ^GT[GC]AC XhoII 963 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### Acc65I 250 GGTACC G^GTACC 444 GGTACC AcyI 95 GGCGCC G[GA]^CG[CT]C 238 GGCGTC AlwI 964 GATCC GGATC(4/5) 963 GGATC AlwNI 611 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG 799 CAGTTTCTG ApaI 689 GGGCCC GGGCC^C 1266 GGGCCC Asp718I 250 GGTACC G^GTACC 444 GGTACC AvaII 29 GGTCC G^G[AT]CC 286 GGACC BsaHI 95 GGCGCC G[GA]^CG[CT]C 238 GGCGTC BsaWI 31 TCCGGT [AT]^CCGG[AT] 283 ACCGGA BsgI 1127 CTGCAC GTGCAG(16/14) 1225 CTGCAC BsmFI 54 GGGAC GGGAC(10/14) 774 GTCCC DraII 688 GGGGCCC [GA]G^G[ACGT]CC[CT] 689 GGGCCCT EcoO109I 688 GGGGCCC [GA]G^G[ACGT]CC[CT] 689 GGGCCCT EcoT14I 533 CCATGG C^C[AT][AT]GG 789 CCTTGG Hin1I 95 GGCGCC G[GA]^CG[CT]C 238 GGCGTC HinfI 56 GACTC G^A[ACGT]TC 707 GAATC KpnI 250 GGTACC GGTAC^C 444 GGTACC MaeIII 278 GTGAC ^GT[ACGT]AC 651 GTTAC StyI 533 CCATGG C^C[AT][AT]GG 789 CCTTGG TaqI 858 TCGA T^CGA 1175 TCGA TthHB8I 858 TCGA T^CGA 1175 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 65 enzymes) # ######################################### AccII AciI AfaI AluI AspHI BanI BanII BbvI BmyI BsaJI BsiHKAI BsiYI BslI Bsp1286I BspMI BsrBI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DsaI EaeI EarI EcoNI EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HpaII HphI ItaI Ksp632I MboI MboII MnlI MslI MspA1I MspI MvaI MvnI MwoI NdeII NlaIII NlaIV PstI RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI TseI TspRI