(Linear) (Six-base) MAPSORT of: KIEE6324.seq Check: 4267 from: 1 to: 1314 KIEE6324.orf With 182 enzymes: SgfI * March 16, 2015 11:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 911 1154 1314 Size: 911 243 160 Fragments arranged by size: 911 243 160 Acc65I G'GTAC_C Cuts at: 0 250 444 1314 Size: 250 194 870 Fragments arranged by size: 870 250 194 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 706 1038 1314 Size: 706 332 276 Fragments arranged by size: 706 332 276 AfeI AGC'GCT Cuts at: 0 577 1314 Size: 577 737 AlwNI CAG_nnn'CTG Cuts at: 0 616 804 1314 Size: 616 188 510 Fragments arranged by size: 616 510 188 ApaI G_GGCC'C Cuts at: 0 693 1270 1314 Size: 693 577 44 Fragments arranged by size: 693 577 44 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 280 313 1314 Size: 280 33 1001 Fragments arranged by size: 1001 280 33 BamHI G'GATC_C Cuts at: 0 963 1314 Size: 963 351 BanI G'GyrC_C Cuts at: 0 95 250 444 1314 Size: 95 155 194 870 Fragments arranged by size: 870 194 155 95 BanII G_rGCy'C Cuts at: 0 693 1172 1181 1270 1314 Size: 693 479 9 89 44 Fragments arranged by size: 693 479 89 44 9 BbeI G_GCGC'C Cuts at: 0 99 1314 Size: 99 1215 BbsI GAAGACnn'nnnn_ Cuts at: 0 408 1314 Size: 408 906 BbvCI CC'TCA_GC Cuts at: 0 958 1314 Size: 958 356 BglI GCCn_nnn'nGGC Cuts at: 0 223 1314 Size: 223 1091 Bme1580I G_kGCm'C Cuts at: 0 693 1270 1314 Size: 693 577 44 Fragments arranged by size: 693 577 44 BmrI ACTGGGnnnn_n' Cuts at: 0 520 1314 Size: 520 794 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 772 1314 Size: 772 542 Bpu10I CC'TnA_GC Cuts at: 0 958 1150 1314 Size: 958 192 164 Fragments arranged by size: 958 192 164 BsaHI Gr'CG_yC Cuts at: 0 96 239 1314 Size: 96 143 1075 Fragments arranged by size: 1075 143 96 BsaWI w'CCGG_w Cuts at: 0 31 283 1314 Size: 31 252 1031 Fragments arranged by size: 1031 252 31 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 655 1314 Size: 655 659 BseYI C'CCAG_C Cuts at: 0 7 300 572 578 584 702 732 760 Size: 7 293 272 6 6 118 30 28 Cuts at: 760 989 1164 1263 1314 Size: 229 175 99 51 Fragments arranged by size: 293 272 229 175 118 99 51 30 28 7 6 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1112 1210 1314 Size: 1112 98 104 Fragments arranged by size: 1112 104 98 BsiEI CG_ry'CG Cuts at: 0 27 1314 Size: 27 1287 BsiHKAI G_wGCw'C Cuts at: 0 392 1181 1291 1314 Size: 392 789 110 23 Fragments arranged by size: 789 392 110 23 Bsp1286I G_dGCh'C Cuts at: 0 392 693 1172 1181 1270 1291 1314 Size: 392 301 479 9 89 21 23 Fragments arranged by size: 479 392 301 89 23 21 9 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 445 911 1154 1314 Size: 445 466 243 160 Fragments arranged by size: 466 445 243 160 BsrBI CCG'CTC Cuts at: 0 93 779 1061 1314 Size: 93 686 282 253 Fragments arranged by size: 686 282 253 93 BsrDI GCAATG_nn' Cuts at: 0 73 1314 Size: 73 1241 BsrFI r'CCGG_y Cuts at: 0 26 1314 Size: 26 1288 BsrGI T'GTAC_A Cuts at: 0 314 1314 Size: 314 1000 BssHII G'CGCG_C Cuts at: 0 78 1314 Size: 78 1236 BstAPI GCAn_nnn'nTGC Cuts at: 0 72 1314 Size: 72 1242 BstYI r'GATC_y Cuts at: 0 963 1314 Size: 963 351 BtgI C'CryG_G Cuts at: 0 38 533 684 1314 Size: 38 495 151 630 Fragments arranged by size: 630 495 151 38 BtsI GCAGTG_nn' Cuts at: 0 1099 1314 Size: 1099 215 DraIII CAC_nnn'GTG Cuts at: 0 189 1314 Size: 189 1125 EaeI y'GGCC_r Cuts at: 0 24 134 224 530 792 1314 Size: 24 110 90 306 262 522 Fragments arranged by size: 522 306 262 110 90 24 EagI C'GGCC_G Cuts at: 0 24 1314 Size: 24 1290 EarI CTCTTCn'nnn_ Cuts at: 0 520 550 643 853 1030 1314 Size: 520 30 93 210 177 284 Fragments arranged by size: 520 284 210 177 93 30 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 169 1314 Size: 169 1145 EcoICRI GAG'CTC Cuts at: 0 1179 1314 Size: 1179 135 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 706 772 1038 1314 Size: 706 66 266 276 Fragments arranged by size: 706 276 266 66 EcoNI CCTnn'n_nnAGG Cuts at: 0 154 838 972 1314 Size: 154 684 134 342 Fragments arranged by size: 684 342 154 134 EcoO109I rG'GnC_Cy Cuts at: 0 689 690 1314 Size: 689 1 624 Fragments arranged by size: 689 624 1 HaeII r_GCGC'y Cuts at: 0 99 579 831 847 1314 Size: 99 480 252 16 467 Fragments arranged by size: 480 467 252 99 16 HincII GTy'rAC Cuts at: 0 881 1314 Size: 881 433 KasI G'GCGC_C Cuts at: 0 95 1314 Size: 95 1219 KpnI G_GTAC'C Cuts at: 0 254 448 1314 Size: 254 194 866 Fragments arranged by size: 866 254 194 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1058 1314 Size: 1058 256 MscI TGG'CCA Cuts at: 0 532 1314 Size: 532 782 MslI CAynn'nnrTG Cuts at: 0 322 364 502 1314 Size: 322 42 138 812 Fragments arranged by size: 812 322 138 42 MspA1I CmG'CkG Cuts at: 0 40 304 764 1314 Size: 40 264 460 550 Fragments arranged by size: 550 460 264 40 NarI GG'CG_CC Cuts at: 0 96 1314 Size: 96 1218 NcoI C'CATG_G Cuts at: 0 533 1314 Size: 533 781 NdeI CA'TA_TG Cuts at: 0 1231 1314 Size: 1231 83 PasI CC'CwG_GG Cuts at: 0 646 1314 Size: 646 668 PfoI T'CCnGG_A Cuts at: 0 1183 1314 Size: 1183 131 PspOMI G'GGCC_C Cuts at: 0 689 1266 1314 Size: 689 577 48 Fragments arranged by size: 689 577 48 PstI C_TGCA'G Cuts at: 0 77 275 922 1213 1256 1314 Size: 77 198 647 291 43 58 Fragments arranged by size: 647 291 198 77 58 43 PvuII CAG'CTG Cuts at: 0 304 1314 Size: 304 1010 RsrII CG'GwC_CG Cuts at: 0 29 1314 Size: 29 1285 SacI G_AGCT'C Cuts at: 0 1181 1314 Size: 1181 133 SacII CC_GC'GG Cuts at: 0 41 1314 Size: 41 1273 SapI GCTCTTCn'nnn_ Cuts at: 0 520 550 643 853 1314 Size: 520 30 93 210 461 Fragments arranged by size: 520 461 210 93 30 SfcI C'TryA_G Cuts at: 0 73 271 918 1209 1252 1314 Size: 73 198 647 291 43 62 Fragments arranged by size: 647 291 198 73 62 43 SfiI GGCCn_nnn'nGGCC Cuts at: 0 223 1314 Size: 223 1091 SfoI GGC'GCC Cuts at: 0 97 1314 Size: 97 1217 StuI AGG'CCT Cuts at: 0 217 1314 Size: 217 1097 StyI C'CwwG_G Cuts at: 0 533 789 1314 Size: 533 256 525 Fragments arranged by size: 533 525 256 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1017 1306 1314 Size: 1017 289 8 Fragments arranged by size: 1017 289 8 TatI w'GTAC_w Cuts at: 0 314 332 1314 Size: 314 18 982 Fragments arranged by size: 982 314 18 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 278 310 1314 Size: 278 32 1004 Fragments arranged by size: 1004 278 32 Enzymes that do cut: AarI Acc65I AcuI AfeI AlwNI ApaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BglI Bme1580I BmrI BpmI Bpu10I BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BstAPI BstYI BtgI BtsI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII HincII KasI KpnI MmeI MscI MslI MspA1I NarI NcoI NdeI PasI PfoI PspOMI PstI PvuII RsrII SacI SacII SapI SfcI SfiI SfoI StuI StyI TaqII TatI TstI Enzymes that do not cut: AatII AccI AclI AflII AflIII AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BcgI BciVI BclI BfrBI BglII BlpI BmgBI BmtI BplI BpuEI BsaI BsaAI BsaBI BsaXI BsiWI BsmI BsmBI BspEI BspHI BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI EcoRI EcoRV FalI FseI FspI FspAI Hin4I HindIII HpaI MfeI MluI NaeI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI SalI SanDI SbfI ScaI SexAI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI