################################## # NO CUT ENZYMES ( 144 enzymes) # ################################## AatII Acc65I AccI AccII AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AvaI AviII AvrII BamHI BanI BanII BbeI BbrPI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsiEI BsiWI BsmBI BsmFI BsoBI BspDI BspLU11I BspMI BsrDI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HpaI KasI KpnI KspI MaeI MaeII MamI MfeI MluI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I StuI SwaI Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AccIII 888 TCCGGA T^CCGGA AlwNI 1199 CAGCGACTG CAG[ACGT][ACGT][ACGT]^CTG AspHI 651 GAGCAC G[AT]GC[AT]^C AspI 858 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 575 TGGCCA TGG^CCA BbvCI 568 CCTCAGC CCTCAGC(-5/-2) BcgI 506 GCATGCAGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BmyI 651 GAGCAC G[AGT]GC[ACT]^C BpmI 1144 CTCCAG CTGGAG(16/14) BsaI 1231 GGTCTC GGTCTC(1/5) BsaWI 888 TCCGGA [AT]^CCGG[AT] BseAI 888 TCCGGA T^CCGGA BseRI 436 GAGGAG GAGGAG(10/8) BsgI 1159 CTGCAC GTGCAG(16/14) BsiHKAI 651 GAGCAC G[AT]GC[AT]^C BsmAI 1232 GTCTC GTCTC(1/5) Bsp1286I 651 GAGCAC G[AGT]GC[ACT]^C Bsp1407I 14 TGTACA T^GTACA BspEI 888 TCCGGA T^CCGGA BspHI 843 TCATGA T^CATGA BsrBI 36 GAGCGG CCGCTC(-3/-3) BsrGI 14 TGTACA T^GTACA BstXI 224 CCAGAAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraI 672 TTTAAA TTT^AAA DraII 295 AGGACCC [GA]G^G[ACGT]CC[CT] DraIII 1162 CACGAAGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 575 TGGCCA [CT]^GGCC[GA] EcoNI 897 CCTCTGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 295 AGGACCC [GA]G^G[ACGT]CC[CT] EcoRI 720 GAATTC G^AATTC EcoRV 1282 GATATC GAT^ATC EcoT22I 504 ATGCAT ATGCA^T HapII 889 CCGG C^CGG HindIII 951 AAGCTT A^AGCTT HpaII 889 CCGG C^CGG MluNI 575 TGGCCA TGG^CCA MroI 888 TCCGGA T^CCGGA MscI 575 TGGCCA TGG^CCA MslI 129 CATACAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 889 CCGG C^CGG NsiI 504 ATGCAT ATGCA^T NspI 506 GCATGC [GA]CATG^[CT] PflFI 858 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 295 AGGACCC [GA]G^G[AT]CC[CT] PvuII 571 CAGCTG CAG^CTG RcaI 843 TCATGA T^CATGA SphI 506 GCATGC GCATG^C SspBI 14 TGTACA T^GTACA TaqI 51 TCGA T^CGA TfiI 91 GATTC G^A[AT]TC Tth111I 858 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 51 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AfaI 15 GTAC GT^AC 938 GTAC BbsI 489 GAAGAC GAAGAC(2/6) 976 GAAGAC BpuAI 489 GAAGAC GAAGAC(2/6) 976 GAAGAC BsrI 418 ACTGG ACTGG(1/-1) 1030 CCAGT BstF5I 604 CATCC GGATG(2/0) 1176 GGATG BstYI 667 GGATCT [GA]^GATC[CT] 1077 GGATCT EarI 1129 GAAGAG CTCTTC(1/4) 1224 GAAGAG FokI 604 CATCC GGATG(9/13) 1176 GGATG Ksp632I 1129 GAAGAG CTCTTC(1/4) 1224 GAAGAG MflI 667 GGATCT [GA]^GATC[CT] 1077 GGATCT MspA1I 244 CAGCGG C[AC]G^C[GT]G 571 CAGCTG PstI 740 CTGCAG CTGCA^G 753 CTGCAG RsaI 15 GTAC GT^AC 938 GTAC SfaNI 103 GATGC GCATC(5/9) 1009 GCATC SmlI 583 CTCAAG C^T[CT][GA]AG 876 CTCAAG SspI 75 AATATT AAT^ATT 1090 AATATT XhoII 667 GGATCT [GA]^GATC[CT] 1077 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 45 enzymes) # ######################################### AciI AcsI AluI AlwI ApoI AvaII BbvI BsaJI BsiYI BslI BsmI BssKI BstNI Cac8I Cfr13I DdeI DpnII EcoRII EcoT14I Fnu4HI HaeIII HinfI HphI ItaI MaeIII MboI MboII MnlI MseI MvaI MwoI NdeII NlaIII NlaIV PleI Sau3AI Sau96I ScrFI SfcI StyI Tru9I TseI Tsp45I Tsp509I TspRI