Restriction map for
pFN21AE6323
(Linear) (Six-base) MAPSORT of: KIEE6323.seq Check: 4005 from: 1 to: 1320
KIEE6323.orf
With 182 enzymes: SgfI *
March 16, 2015 11:29 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 119 238 508 1226 1243 1320
Size: 119 119 270 718 17 77
Fragments arranged by size:
718 270 119 119 77 17
AlwNI CAG_nnn'CTG
Cuts at: 0 1204 1320
Size: 1204 116
ApoI r'AATT_y
Cuts at: 0 149 720 1237 1320
Size: 149 571 517 83
Fragments arranged by size:
571 517 149 83
BbsI GAAGACnn'nnnn_
Cuts at: 0 496 983 1320
Size: 496 487 337
Fragments arranged by size:
496 487 337
BbvCI CC'TCA_GC
Cuts at: 0 569 1320
Size: 569 751
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 495 529 1320
Size: 495 34 791
Fragments arranged by size:
791 495 34
BfrBI ATG'CAT
Cuts at: 0 506 1320
Size: 506 814
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1129 1320
Size: 1129 191
Bpu10I CC'TnA_GC
Cuts at: 0 569 1320
Size: 569 751
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 568 861 1320
Size: 568 293 459
Fragments arranged by size:
568 459 293
BsaI GGTCTCn'nnnn_
Cuts at: 0 1237 1320
Size: 1237 83
BsaWI w'CCGG_w
Cuts at: 0 888 1320
Size: 888 432
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 298 328 1320
Size: 298 30 992
Fragments arranged by size:
992 298 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 451 1320
Size: 451 869
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1144 1320
Size: 1144 176
BsiHKAI G_wGCw'C
Cuts at: 0 655 1320
Size: 655 665
BsmI GAATG_Cn'
Cuts at: 0 508 898 986 1000 1320
Size: 508 390 88 14 320
Fragments arranged by size:
508 390 320 88 14
Bsp1286I G_dGCh'C
Cuts at: 0 655 1320
Size: 655 665
BspEI T'CCGG_A
Cuts at: 0 888 1320
Size: 888 432
BspHI T'CATG_A
Cuts at: 0 843 1320
Size: 843 477
BsrBI CCG'CTC
Cuts at: 0 38 1320
Size: 38 1282
BsrGI T'GTAC_A
Cuts at: 0 14 1320
Size: 14 1306
BstXI CCAn_nnnn'nTGG
Cuts at: 0 231 1320
Size: 231 1089
BstYI r'GATC_y
Cuts at: 0 667 1077 1320
Size: 667 410 243
Fragments arranged by size:
667 410 243
DraI TTT'AAA
Cuts at: 0 674 1320
Size: 674 646
DraIII CAC_nnn'GTG
Cuts at: 0 1167 1320
Size: 1167 153
EaeI y'GGCC_r
Cuts at: 0 575 1320
Size: 575 745
EarI CTCTTCn'nnn_
Cuts at: 0 1124 1219 1320
Size: 1124 95 101
Fragments arranged by size:
1124 101 95
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1189 1320
Size: 1189 131
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 119 238 508 1129 1226 1243 1320
Size: 119 119 270 621 97 17 77
Fragments arranged by size:
621 270 119 119 97 77 17
EcoNI CCTnn'n_nnAGG
Cuts at: 0 901 1320
Size: 901 419
EcoO109I rG'GnC_Cy
Cuts at: 0 296 1320
Size: 296 1024
EcoRI G'AATT_C
Cuts at: 0 720 1320
Size: 720 600
EcoRV GAT'ATC
Cuts at: 0 1284 1320
Size: 1284 36
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 741 1320
Size: 741 579
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 15 47 588 620 1320
Size: 15 32 541 32 700
Fragments arranged by size:
700 541 32 32 15
HindIII A'AGCT_T
Cuts at: 0 951 1320
Size: 951 369
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1065 1320
Size: 1065 255
MscI TGG'CCA
Cuts at: 0 577 1320
Size: 577 743
MslI CAynn'nnrTG
Cuts at: 0 133 1320
Size: 133 1187
MspA1I CmG'CkG
Cuts at: 0 246 573 1320
Size: 246 327 747
Fragments arranged by size:
747 327 246
NsiI A_TGCA'T
Cuts at: 0 508 1320
Size: 508 812
NspI r_CATG'y
Cuts at: 0 510 1320
Size: 510 810
PasI CC'CwG_GG
Cuts at: 0 196 261 1320
Size: 196 65 1059
Fragments arranged by size:
1059 196 65
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 347 379 1320
Size: 347 32 941
Fragments arranged by size:
941 347 32
PpuMI rG'GwC_Cy
Cuts at: 0 296 1320
Size: 296 1024
PstI C_TGCA'G
Cuts at: 0 744 757 1320
Size: 744 13 563
Fragments arranged by size:
744 563 13
PvuII CAG'CTG
Cuts at: 0 573 1320
Size: 573 747
SfcI C'TryA_G
Cuts at: 0 164 740 753 1052 1320
Size: 164 576 13 299 268
Fragments arranged by size:
576 299 268 164 13
SmlI C'TyrA_G
Cuts at: 0 583 876 1320
Size: 583 293 444
Fragments arranged by size:
583 444 293
SphI G_CATG'C
Cuts at: 0 510 1320
Size: 510 810
SspI AAT'ATT
Cuts at: 0 77 1092 1320
Size: 77 1015 228
Fragments arranged by size:
1015 228 77
StyI C'CwwG_G
Cuts at: 0 272 494 798 1320
Size: 272 222 304 522
Fragments arranged by size:
522 304 272 222
TatI w'GTAC_w
Cuts at: 0 14 1320
Size: 14 1306
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 434 466 1320
Size: 434 32 854
Fragments arranged by size:
854 434 32
Tth111I GACn'n_nGTC
Cuts at: 0 861 1320
Size: 861 459
Enzymes that do cut:
AcuI AlwNI ApoI BbsI BbvCI BcgI BfrBI BpmI
Bpu10I BpuEI BsaI BsaWI BsaXI BseRI BsgI BsiHKAI
BsmI Bsp1286I BspEI BspHI BsrBI BsrGI BstXI BstYI
DraI DraIII EaeI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI Hin4I HindIII MmeI MscI MslI
MspA1I NsiI NspI PasI PpiI PpuMI PstI PvuII
SfcI SmlI SphI SspI StyI TatI TstI Tth111I
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbeI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BsaAI BsaBI BsaHI BseYI BsiEI
BsiWI BsmBI BspMI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstEII BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DrdI EagI EcoICRI FseI FspI FspAI HaeII
HincII HpaI KasI KpnI MfeI MluI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI PacI PciI
PflMI PfoI PmeI PmlI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SrfI StuI SwaI TaqII XbaI XcmI
XhoI XmaI XmnI ZraI