################################## # NO CUT ENZYMES ( 115 enzymes) # ################################## Acc65I AccIII AclI AcsI AflII AgeI Alw44I AocI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AviII AvrII BalI BbrPI BcgI BciVI BclI BfaI BfrI BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BsgI BsiWI BsmI BspDI BspEI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DrdI Eco81I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HincII HindII HindIII HinfI HpaI KpnI MaeI MamI MfeI MluI MluNI MroI MscI MunI NcoI NdeI NheI NotI NruI NsiI NspV PacI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RsrII SacI SalI SbfI SexAI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI StyI SwaI TfiI Tsp509I Van91I XbaI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AatII 808 GACGTC GACGT^C AccI 118 GTCTAC GT^[AC][GT]AC AhdI 727 GACGAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 97 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG Asp700I 45 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 727 GACGAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 1140 GAGCAC G[AT]GC[AT]^C AspI 805 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 611 GGATCC G^GATCC BbeI 891 GGCGCC GGCGC^C BbvCI 954 CCTCAGC CCTCAGC(-5/-2) BsiHKAI 1140 GAGCAC G[AT]GC[AT]^C BsmBI 652 GAGACG CGTCTC(1/5) Bsp1407I 74 TGTACA T^GTACA BspHI 302 TCATGA T^CATGA BspLU11I 77 ACATGT A^CATGT BsrDI 489 CATTGC GCAATG(2/0) BsrGI 74 TGTACA T^GTACA DraII 98 AGGACCT [GA]G^G[ACGT]CC[CT] DraIII 67 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 727 GACGAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 561 GAAGAG CTCTTC(1/4) EcoO109I 98 AGGACCT [GA]G^G[ACGT]CC[CT] KasI 891 GGCGCC G^GCGCC Ksp632I 561 GAAGAG CTCTTC(1/4) KspI 1099 CCGCGG CCGC^GG MseI 160 TTAA T^TAA NaeI 1086 GCCGGC GCC^GGC NarI 891 GGCGCC GG^CGCC NgoMIV 1086 GCCGGC G^CCGGC NspI 77 ACATGT [GA]CATG^[CT] PflFI 805 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 98 AGGACCT [GA]G^G[AT]CC[CT] RcaI 302 TCATGA T^CATGA SacII 1099 CCGCGG CCGC^GG SapI 561 GAAGAGC GCTCTTC(1/4) ScaI 287 AGTACT AGT^ACT SfoI 891 GGCGCC GGC^GCC SmaI 1036 CCCGGG CCC^GGG SspBI 74 TGTACA T^GTACA StuI 704 AGGCCT AGG^CCT Tru9I 160 TTAA T^TAA TspRI 711 CACTG CA[GC]TG[ACGT][ACGT]^ Tth111I 805 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC XmaCI 1036 CCCGGG C^CCGGG XmaI 1036 CCCGGG C^CCGGG XmnI 45 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AflIII 77 ACATGT A^C[GA][CT]GT 857 ACGTGT Aor51HI 47 AGCGCT AGC^GCT 1070 AGCGCT AvaII 99 GGACC G^G[AT]CC 1024 GGACC BanI 891 GGCGCC G^G[CT][GA]CC 1092 GGTGCC BbsI 504 GAAGAC GAAGAC(2/6) 658 GAAGAC BglI 697 GCCATCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1095 GCCCCCGCGGC BglII 137 AGATCT A^GATCT 320 AGATCT BpuAI 504 GAAGAC GAAGAC(2/6) 658 GAAGAC BsmAI 409 GAGAC GTCTC(1/5) 652 GAGAC DdeI 665 CTGAG C^T[ACGT]AG 955 CTCAG EaeI 1018 CGGCCG [CT]^GGCC[GA] 1102 CGGCCG EagI 1018 CGGCCG C^GGCCG 1102 CGGCCG EclXI 1018 CGGCCG C^GGCCG 1102 CGGCCG Eco47III 47 AGCGCT AGC^GCT 1070 AGCGCT Eco52I 1018 CGGCCG C^GGCCG 1102 CGGCCG EcoNI 738 CCTGCTCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 954 CCTCAGCCAGG MaeIII 20 GTCAC ^GT[ACGT]AC 811 GTCAC MslI 297 CACGCTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 711 CACTGCTGTG SfaNI 420 GATGC GCATC(5/9) 929 GCATC Tsp45I 20 GTCAC ^GT[GC]AC 811 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 76 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AvaI BanII BbvI BcnI BmyI BsaHI BsaJI BseRI BsiEI BsiYI BslI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DpnII DsaI EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HpaII HphI ItaI MaeII MboI MboII MflI MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PaeR7I PstI RsaI Sau3AI Sau96I ScrFI SfcI SmlI TaqI TseI TthHB8I XcmI XhoI XhoII