(Circular) (Six-base) MAPSORT of: pFN21AE1365.seq Check: 4693 from: 1 to: 5875 pFN21AE1365 5875 bp With 182 enzymes: SgfI * August 16, 2010 14:46 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2675 2675 Size: 5875 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2817 3685 278 Size: 53 83 186 936 1281 868 2468 Fragments arranged by size: 2468 1281 936 868 186 83 53 AccI GT'mk_AC Cuts at: 1937 2124 3218 1937 Size: 187 1094 4594 Fragments arranged by size: 4594 1094 187 Acc65I G'GTAC_C Cuts at: 3196 3196 Size: 5875 AclI AA'CG_TT Cuts at: 1563 4002 4375 1563 Size: 2439 373 3063 Fragments arranged by size: 3063 2439 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3938 5322 5590 1632 Size: 2306 1384 268 1917 Fragments arranged by size: 2306 1917 1384 268 AfeI AGC'GCT Cuts at: 2054 3077 2054 Size: 1023 4852 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5678 AflIII A'CryG_T Cuts at: 1203 2082 2862 4774 5751 1203 Size: 879 780 1912 977 1327 Fragments arranged by size: 1912 1327 977 879 780 AgeI A'CCGG_T Cuts at: 4706 4706 Size: 5875 AhdI GACnn_n'nnGTC Cuts at: 2737 4604 2737 Size: 1867 4008 AleI CACnn'nnGTG Cuts at: 1166 2720 1166 Size: 1554 4321 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5875 AlwNI CAG_nnn'CTG Cuts at: 2107 3618 5190 2107 Size: 1511 1572 2792 Fragments arranged by size: 2792 1572 1511 ApaLI G'TGCA_C Cuts at: 3932 5088 3932 Size: 1156 4719 ApoI r'AATT_y Cuts at: 1493 3184 3410 3562 5671 1493 Size: 1691 226 152 2109 1697 Fragments arranged by size: 2109 1697 1691 226 152 AseI AT'TA_AT Cuts at: 160 1032 4429 160 Size: 872 3397 1606 Fragments arranged by size: 3397 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5875 AvaI C'yCGr_G Cuts at: 1870 1943 2515 2633 2747 3041 3053 3200 1870 Size: 73 572 118 114 294 12 147 4545 Fragments arranged by size: 4545 572 294 147 118 114 73 12 BamHI G'GATC_C Cuts at: 2616 3205 3572 2616 Size: 589 367 4919 Fragments arranged by size: 4919 589 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2896 3097 3196 4651 618 Size: 325 217 627 1109 201 99 1455 1842 Fragments arranged by size: 1842 1455 1109 627 325 217 201 99 BanII G_rGCy'C Cuts at: 1391 2522 2640 2676 3041 3050 3194 1391 Size: 1131 118 36 365 9 144 4072 Fragments arranged by size: 4072 1131 365 144 118 36 9 BbeI G_GCGC'C Cuts at: 2900 2900 Size: 5875 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2516 2670 928 Size: 971 617 154 4133 Fragments arranged by size: 4133 971 617 154 BbvCI CC'TCA_GC Cuts at: 2960 2960 Size: 5875 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4066 4100 1903 Size: 34 2129 34 3678 Fragments arranged by size: 3678 2129 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3767 4977 3767 Size: 1210 4665 BclI T'GATC_A Cuts at: 1548 1548 Size: 5875 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2708 3106 4486 Size: 107 122 71 631 605 1036 398 1380 Cuts at: 4486 136 Size: 1525 Fragments arranged by size: 1525 1380 1036 631 605 398 122 107 71 BglII A'GATC_T Cuts at: 2142 2325 5870 2142 Size: 183 3545 2147 Fragments arranged by size: 3545 2147 183 BlpI GC'TnA_GC Cuts at: 3292 3292 Size: 5875 Bme1580I G_kGCm'C Cuts at: 1790 2282 3102 3936 5092 1790 Size: 492 820 834 1156 2573 Fragments arranged by size: 2573 1156 834 820 492 BmgBI CAC'GTC Cuts at: 1318 2548 2863 1318 Size: 1230 315 4330 Fragments arranged by size: 4330 1230 315 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3606 4564 442 Size: 416 53 83 399 24 2189 958 1753 Fragments arranged by size: 2189 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5875 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2530 2530 Size: 5875 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4535 1239 Size: 3296 2579 Bpu10I CC'TnA_GC Cuts at: 2670 2960 2670 Size: 290 5585 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2363 4000 4865 5163 2363 Size: 1637 865 298 3075 Fragments arranged by size: 3075 1637 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4538 882 Size: 617 3039 2219 Fragments arranged by size: 3039 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3666 493 Size: 711 2462 2702 Fragments arranged by size: 2702 2462 711 BsaBI GATnn'nnATC Cuts at: 5869 5869 Size: 5875 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2814 2897 2970 3010 Size: 53 83 186 936 1281 83 73 40 Cuts at: 3010 3164 3682 4064 4699 275 Size: 154 518 382 635 1451 Fragments arranged by size: 1451 1281 936 635 518 382 186 154 83 83 73 53 40 BsaWI w'CCGG_w Cuts at: 4308 4706 4980 5127 5538 4308 Size: 398 274 147 411 4645 Fragments arranged by size: 4645 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5650 Fragments arranged by size: 5650 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2336 3071 3074 1186 Size: 1150 735 3 3987 Fragments arranged by size: 3987 1150 735 3 BseYI C'CCAG_C Cuts at: 2038 2063 2230 2620 5078 5574 2038 Size: 25 167 390 2458 496 2339 Fragments arranged by size: 2458 2339 496 390 167 25 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5302 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2948 3026 3110 3325 4086 5114 Size: 275 186 947 78 84 215 761 1028 Cuts at: 5114 1540 Size: 2301 Fragments arranged by size: 2301 1028 947 761 275 215 186 84 78 BsiHKAI G_wGCw'C Cuts at: 3149 3936 4021 5092 3149 Size: 787 85 1071 3932 Fragments arranged by size: 3932 1071 787 85 BsmI GAATG_Cn' Cuts at: 3386 3479 3386 Size: 93 5782 BsmBI CGTCTCn'nnnn_ Cuts at: 2651 2651 Size: 5875 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2282 2522 2640 2676 3041 3050 3102 Size: 399 492 240 118 36 365 9 52 Cuts at: 3102 3149 3194 3936 4021 5092 1391 Size: 47 45 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 492 399 365 240 118 85 52 47 45 36 9 BspEI T'CCGG_A Cuts at: 5538 5538 Size: 5875 BspHI T'CATG_A Cuts at: 2307 3764 5494 2307 Size: 1457 1730 2688 Fragments arranged by size: 2688 1730 1457 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2675 2850 2898 3230 844 Size: 1831 175 48 332 3489 Fragments arranged by size: 3489 1831 332 175 48 BsrBI CCG'CTC Cuts at: 1394 1823 2577 3052 3064 3762 1394 Size: 429 754 475 12 698 3507 Fragments arranged by size: 3507 754 698 475 429 12 BsrDI GCAATG_nn' Cuts at: 65 1248 2493 4370 4544 65 Size: 1183 1245 1877 174 1396 Fragments arranged by size: 1877 1396 1245 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2127 2462 3091 4519 4706 1700 Size: 110 317 335 629 1428 187 2869 Fragments arranged by size: 2869 1428 629 335 317 187 110 BsrGI T'GTAC_A Cuts at: 96 2079 96 Size: 1983 3892 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5875 BssSI C'ACGA_G Cuts at: 3935 4947 3935 Size: 1012 4863 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5875 BstBI TT'CG_AA Cuts at: 4679 4679 Size: 5875 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5875 BstYI r'GATC_y Cuts at: 1972 2142 2325 2616 3205 3572 3957 3974 5426 Size: 170 183 291 589 367 385 17 1452 Cuts at: 5426 5512 5524 5870 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 589 385 367 346 291 183 170 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2388 2538 3104 513 Size: 552 939 384 150 566 3284 Fragments arranged by size: 3284 939 566 552 384 150 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2014 487 Size: 683 56 788 4348 Fragments arranged by size: 4348 788 683 56 BtsI GCAGTG_nn' Cuts at: 2715 3391 4185 4205 2715 Size: 676 794 20 4385 Fragments arranged by size: 4385 794 676 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5875 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5840 DraI TTT'AAA Cuts at: 3179 3179 Size: 5875 DraIII CAC_nnn'GTG Cuts at: 2077 2077 Size: 5875 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4882 809 Size: 507 3566 1802 Fragments arranged by size: 3566 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3023 3107 3322 Size: 54 1425 50 275 18 1193 84 215 Cuts at: 3322 4211 5840 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1193 889 275 215 84 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3023 3107 3322 1537 Size: 275 1211 84 215 4090 Fragments arranged by size: 4090 1211 275 215 84 EarI CTCTTCn'nnn_ Cuts at: 1353 2561 3805 1353 Size: 1208 1244 3423 Fragments arranged by size: 3423 1244 1208 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4462 4836 4982 387 Size: 1064 138 2873 374 146 1280 Fragments arranged by size: 2873 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3938 4535 5322 5590 1239 Size: 393 2306 597 787 268 1524 Fragments arranged by size: 2306 1524 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2747 2963 2747 Size: 216 5659 EcoO109I rG'GnC_Cy Cuts at: 2104 2104 Size: 5875 EcoRI G'AATT_C Cuts at: 3184 3562 3184 Size: 378 5497 FspI TGC'GCA Cuts at: 4381 4381 Size: 5875 HaeII r_GCGC'y Cuts at: 1678 2056 2134 2900 2959 3079 3117 5022 1678 Size: 378 78 766 59 120 38 1905 2531 Fragments arranged by size: 2531 1905 766 378 120 78 59 38 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2601 2633 2723 2755 4516 Size: 32 135 32 1029 32 90 32 1761 Cuts at: 4516 4548 4590 4622 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1761 1029 135 90 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3219 3465 669 Size: 988 281 1281 246 3079 Fragments arranged by size: 3079 1281 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5875 HpaI GTT'AAC Cuts at: 3465 3465 Size: 5875 KasI G'GCGC_C Cuts at: 2896 2896 Size: 5875 KpnI G_GTAC'C Cuts at: 3200 3200 Size: 5875 MfeI C'AATT_G Cuts at: 3474 3474 Size: 5875 MluI A'CGCG_T Cuts at: 5751 5751 Size: 5875 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2091 4989 5173 1305 Size: 786 2898 184 2007 Fragments arranged by size: 2898 2007 786 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4053 Fragments arranged by size: 4053 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2306 2720 3833 4192 4351 518 Size: 648 1140 414 1113 359 159 2042 Fragments arranged by size: 2042 1140 1113 648 414 359 159 MspA1I CmG'CkG Cuts at: 1606 2257 2334 2944 3106 3135 3291 3968 5116 Size: 651 77 610 162 29 156 677 1148 Cuts at: 5116 5361 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 651 610 245 162 156 77 29 NaeI GCC'GGC Cuts at: 3093 3093 Size: 5875 NarI GG'CG_CC Cuts at: 2897 2897 Size: 5875 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4384 Fragments arranged by size: 4384 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5875 NgoMIV G'CCGG_C Cuts at: 3091 3091 Size: 5875 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5875 NotI GC'GGCC_GC Cuts at: 3322 3322 Size: 5875 NspI r_CATG'y Cuts at: 1132 1229 2086 3233 4778 1132 Size: 97 857 1147 1545 2229 Fragments arranged by size: 2229 1545 1147 857 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5875 PciI A'CATG_T Cuts at: 2082 4774 2082 Size: 2692 3183 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5875 PfoI T'CCnGG_A Cuts at: 2781 2781 Size: 5875 PmeI GTTT'AAAC Cuts at: 3179 3179 Size: 5875 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3944 3976 5457 5489 3944 Size: 32 1481 32 4330 Fragments arranged by size: 4330 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2104 2104 Size: 5875 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5875 PsiI TTA'TAA Cuts at: 3445 3445 Size: 5875 PspXI vC'TCGA_Gb Cuts at: 2633 2747 3053 2633 Size: 114 306 5455 Fragments arranged by size: 5455 306 114 PstI C_TGCA'G Cuts at: 830 2300 2481 3003 3140 3227 830 Size: 1470 181 522 137 87 3478 Fragments arranged by size: 3478 1470 522 181 137 87 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5875 SacII CC_GC'GG Cuts at: 3107 3107 Size: 5875 SalI G'TCGA_C Cuts at: 1936 3217 1936 Size: 1281 4594 SapI GCTCTTCn'nnn_ Cuts at: 2561 2561 Size: 5875 SbfI CC_TGCA'GG Cuts at: 3227 3227 Size: 5875 ScaI AGT'ACT Cuts at: 2294 4123 5835 2294 Size: 1829 1712 2334 Fragments arranged by size: 2334 1829 1712 SfcI C'TryA_G Cuts at: 826 1045 2296 2477 2999 3136 3223 4358 5039 Size: 219 1251 181 522 137 87 1135 681 Cuts at: 5039 5230 826 Size: 191 1471 Fragments arranged by size: 1471 1251 1135 681 522 219 191 181 137 87 SfoI GGC'GCC Cuts at: 2898 2898 Size: 5875 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5875 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5875 SmaI CCC'GGG Cuts at: 1872 3043 3202 1872 Size: 1171 159 4545 Fragments arranged by size: 4545 1171 159 SmlI C'TyrA_G Cuts at: 820 1017 1943 2378 2633 2747 3053 3979 4880 Size: 197 926 435 255 114 306 926 901 Cuts at: 4880 5142 820 Size: 262 1553 Fragments arranged by size: 1553 926 926 901 435 306 262 255 197 114 SnaBI TAC'GTA Cuts at: 493 3666 493 Size: 3173 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5875 SphI G_CATG'C Cuts at: 1132 3233 1132 Size: 2101 3774 SspI AAT'ATT Cuts at: 5 52 3799 5 Size: 47 3747 2081 Fragments arranged by size: 3747 2081 47 StuI AGG'CCT Cuts at: 1517 2711 1517 Size: 1194 4681 StyI C'CwwG_G Cuts at: 513 1065 2004 3314 513 Size: 552 939 1310 3074 Fragments arranged by size: 3074 1310 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3902 3919 4072 4257 4702 325 Size: 1255 2322 17 153 185 445 1498 Fragments arranged by size: 2322 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2079 2292 4121 Size: 274 80 33 51 1443 102 213 1829 Cuts at: 4121 5833 96 Size: 1712 138 Fragments arranged by size: 1829 1712 1443 274 213 138 102 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2777 2809 5835 5867 2777 Size: 32 3026 32 2785 Fragments arranged by size: 3026 2785 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2813 1316 Size: 1497 4378 XbaI T'CTAG_A Cuts at: 3211 3211 Size: 5875 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2238 2545 3157 2238 Size: 307 612 4956 Fragments arranged by size: 4956 612 307 XhoI C'TCGA_G Cuts at: 1943 2633 2747 3053 1943 Size: 690 114 306 4765 Fragments arranged by size: 4765 690 306 114 XmaI C'CCGG_G Cuts at: 1870 3041 3200 1870 Size: 1171 159 4545 Fragments arranged by size: 4545 1171 159 XmnI GAAnn'nnTTC Cuts at: 2054 4004 2054 Size: 1950 3925 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2815 3683 276 Size: 53 83 186 936 1281 868 2468 Fragments arranged by size: 2468 1281 936 868 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BfrBI BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspOMI PsrI PvuII RsrII SacI SanDI SexAI SfiI SrfI SwaI