################################## # NO CUT ENZYMES ( 153 enzymes) # ################################## Acc65I AccII AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BamHI BbeI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BsaBI BsaI BsaWI BseAI BsgI BsiHKAI BsiWI BsmAI BsmBI BspDI BspEI BspHI BspLU11I BspMI BsrDI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstYI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HgaI HincII HindII HindIII HpaI KasI KpnI KspI MaeIII MamI MfeI MflI MluI MroI MseI MunI MvnI NaeI NarI NdeI NgoMIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmeI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI TaqI Tru9I Tsp45I Tth111I TthHB8I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AatII 505 GACGTC GACGT^C AccI 508 GTCTAC GT^[AC][GT]AC AcyI 505 GACGTC G[GA]^CG[CT]C AvaI 696 CTCGGG C^[CT]CG[GA]G BalI 272 TGGCCA TGG^CCA BanI 802 GGCACC G^G[CT][GA]CC BbrPI 280 CACGTG CAC^GTG BsaAI 280 CACGTG [CT]AC^GT[GA] BsaHI 505 GACGTC G[GA]^CG[CT]C BsiEI 831 CGACCG CG[GA][CT]^CG BsmI 45 GCATTC GAATGC(1/-1) BsoBI 696 CTCGGG C^[CT]CG[GA]G Bsp1407I 17 TGTACA T^GTACA BsrBI 669 CCGCTC CCGCTC(-3/-3) BsrFI 41 ACCGGC [GA]^CCGG[CT] BsrGI 17 TGTACA T^GTACA BsrI 5 ACTGG ACTGG(1/-1) BssSI 268 CTCGTG CACGAG(-5/-1) Cfr10I 41 ACCGGC [GA]^CCGG[CT] DpnII 741 GATC ^GATC DraII 335 GGGACCC [GA]G^G[ACGT]CC[CT] EarI 360 GAAGAG CTCTTC(1/4) EcoNI 348 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 335 GGGACCC [GA]G^G[ACGT]CC[CT] HaeII 665 AGCGCC [GA]GCGC^[CT] Hin1I 505 GACGTC G[GA]^CG[CT]C HinfI 95 GAATC G^A[ACGT]TC Ksp632I 360 GAAGAG CTCTTC(1/4) MboI 741 GATC ^GATC MluNI 272 TGGCCA TGG^CCA MscI 272 TGGCCA TGG^CCA MspA1I 834 CCGCTG C[AC]G^C[GT]G NdeII 741 GATC ^GATC NheI 582 GCTAGC G^CTAGC PmaCI 280 CACGTG CAC^GTG PmlI 280 CACGTG CAC^GTG PpuMI 335 GGGACCC [GA]G^G[AT]CC[CT] PshAI 399 GACCTATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Sau3AI 741 GATC ^GATC SmlI 322 CTTGAG C^T[CT][GA]AG SspBI 17 TGTACA T^GTACA TfiI 95 GAATC G^A[AT]TC Tsp509I 200 AATT ^AATT XcmI 482 CCATGGTGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AfaI 18 GTAC GT^AC 630 GTAC BanII 659 GAGCCC G[GA]GC[CT]^C 693 GGGCTC BcnI 642 CCGGG CC^[GC]GG 806 CCGGG BfaI 247 CTAG C^TAG 583 CTAG BpmI 166 CTCCAG CTGGAG(16/14) 349 CTGGAG BseRI 265 CTCCTC GAGGAG(10/8) 352 GAGGAG BstXI 275 CCATGCACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 521 CCAACACAGTGG CfoI 666 GCGC GCG^C 789 GCGC DsaI 330 CCATGG C^C[GA][CT]GG 482 CCATGG EcoT14I 330 CCATGG C^C[AT][AT]GG 482 CCATGG HhaI 666 GCGC GCG^C 789 GCGC HinP1I 666 GCGC G^CGC 789 GCGC MaeI 247 CTAG C^TAG 583 CTAG MaeII 281 ACGT A^CGT 506 ACGT NciI 642 CCGGG CC^[GC]GG 806 CCGGG NcoI 330 CCATGG C^CATGG 482 CCATGG PstI 116 CTGCAG CTGCA^G 235 CTGCAG RsaI 18 GTAC GT^AC 630 GTAC SfaNI 596 GCATC GCATC(5/9) 602 GCATC SfcI 116 CTGCAG C^T[GA][CT]AG 235 CTGCAG StyI 330 CCATGG C^C[AT][AT]GG 482 CCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 40 enzymes) # ######################################### AciI AluI AvaII BbsI BbvI BmyI BpuAI BsaJI BsiYI BslI BsmFI Bsp1286I BssKI BstF5I BstNI Cac8I Cfr13I DdeI EaeI EcoRII FauI Fnu4HI FokI HaeIII HapII HpaII HphI ItaI MboII MnlI MslI MspI MvaI MwoI NlaIII NlaIV Sau96I ScrFI TseI TspRI