(Circular) (Six-base) MAPSORT of: pFN21AB7117.seq Check: 7375 from: 1 to: 5554 pFN21AB7117 5554 bp With 182 enzymes: SgfI * August 16, 2010 14:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2514 3364 278 Size: 53 83 186 936 978 850 2468 Fragments arranged by size: 2468 978 936 850 186 83 53 AccI GT'mk_AC Cuts at: 1937 2514 2897 1937 Size: 577 383 4594 Fragments arranged by size: 4594 577 383 Acc65I G'GTAC_C Cuts at: 2875 2875 Size: 5554 AclI AA'CG_TT Cuts at: 1563 3681 4054 1563 Size: 2118 373 3063 Fragments arranged by size: 3063 2118 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2080 3617 5001 5269 1632 Size: 448 1537 1384 268 1917 Fragments arranged by size: 1917 1537 1384 448 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5357 AflIII A'CryG_T Cuts at: 1203 4453 5430 1203 Size: 3250 977 1327 Fragments arranged by size: 3250 1327 977 AgeI A'CCGG_T Cuts at: 4385 4385 Size: 5554 AhdI GACnn_n'nnGTC Cuts at: 4283 4283 Size: 5554 AleI CACnn'nnGTG Cuts at: 1166 2398 1166 Size: 1232 4322 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2073 1184 Size: 889 4665 AlwNI CAG_nnn'CTG Cuts at: 3297 4869 3297 Size: 1572 3982 ApaLI G'TGCA_C Cuts at: 3611 4767 3611 Size: 1156 4398 ApoI r'AATT_y Cuts at: 1493 2863 3089 3241 5350 1493 Size: 1370 226 152 2109 1697 Fragments arranged by size: 2109 1697 1370 226 152 AseI AT'TA_AT Cuts at: 160 1032 4108 160 Size: 872 3076 1606 Fragments arranged by size: 3076 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5554 AvaI C'yCGr_G Cuts at: 1870 1943 2701 2879 1870 Size: 73 758 178 4545 Fragments arranged by size: 4545 758 178 73 BamHI G'GATC_C Cuts at: 2884 3251 2884 Size: 367 5187 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2807 2875 4330 618 Size: 325 217 627 1020 68 1455 1842 Fragments arranged by size: 1842 1455 1020 627 325 217 68 BanII G_rGCy'C Cuts at: 1391 2668 2702 2873 1391 Size: 1277 34 171 4072 Fragments arranged by size: 4072 1277 171 34 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2440 2563 2572 928 Size: 971 541 123 9 3910 Fragments arranged by size: 3910 971 541 123 9 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3745 3779 1903 Size: 34 1808 34 3678 Fragments arranged by size: 3678 1808 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3446 4656 3446 Size: 1210 4344 BclI T'GATC_A Cuts at: 1548 1548 Size: 5554 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4165 136 Size: 107 122 71 631 605 2493 1525 Fragments arranged by size: 2493 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5549 5549 Size: 5554 BlpI GC'TnA_GC Cuts at: 2971 2971 Size: 5554 Bme1580I G_kGCm'C Cuts at: 1790 2659 3615 4771 1790 Size: 869 956 1156 2573 Fragments arranged by size: 2573 1156 956 869 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5554 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3285 4243 442 Size: 416 53 83 399 24 1868 958 1753 Fragments arranged by size: 1868 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2591 1056 Size: 1535 4019 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2093 2093 Size: 5554 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2156 2375 4214 1239 Size: 917 219 1839 2579 Fragments arranged by size: 2579 1839 917 219 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2348 3679 4544 4842 2348 Size: 1331 865 298 3060 Fragments arranged by size: 3060 1331 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4217 882 Size: 617 2718 2219 Fragments arranged by size: 2718 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2287 3345 493 Size: 711 1083 1058 2702 Fragments arranged by size: 2702 1083 1058 711 BsaBI GATnn'nnATC Cuts at: 5548 5548 Size: 5554 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2511 3361 3743 4378 Size: 53 83 186 936 978 850 382 635 Cuts at: 4378 275 Size: 1451 Fragments arranged by size: 1451 978 936 850 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3987 4385 4659 4806 5217 3987 Size: 398 274 147 411 4324 Fragments arranged by size: 4324 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5329 Fragments arranged by size: 5329 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2261 2372 1186 Size: 1075 111 4368 Fragments arranged by size: 4368 1075 111 BseYI C'CCAG_C Cuts at: 2263 2667 2816 4757 5253 2263 Size: 404 149 1941 496 2564 Fragments arranged by size: 2564 1941 496 404 149 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4981 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2839 3004 3765 4793 1540 Size: 275 186 838 165 761 1028 2301 Fragments arranged by size: 2301 1028 838 761 275 186 165 BsiHKAI G_wGCw'C Cuts at: 3615 3700 4771 3615 Size: 85 1071 4398 Fragments arranged by size: 4398 1071 85 BsmI GAATG_Cn' Cuts at: 2050 3065 3158 2050 Size: 1015 93 4446 Fragments arranged by size: 4446 1015 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2659 2668 2702 2873 3615 3700 4771 Size: 399 869 9 34 171 742 85 1071 Cuts at: 4771 1391 Size: 2174 Fragments arranged by size: 2174 1071 869 742 399 171 85 34 9 BspEI T'CCGG_A Cuts at: 5217 5217 Size: 5554 BspHI T'CATG_A Cuts at: 3443 5173 3443 Size: 1730 3824 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2909 844 Size: 2065 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2676 3441 1394 Size: 429 853 765 3507 Fragments arranged by size: 3507 853 765 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4049 4223 65 Size: 1183 2801 174 1396 Fragments arranged by size: 2801 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2046 4198 4385 1700 Size: 110 236 2152 187 2869 Fragments arranged by size: 2869 2152 236 187 110 BsrGI T'GTAC_A Cuts at: 96 2022 96 Size: 1926 3628 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5554 BssSI C'ACGA_G Cuts at: 2273 3614 4626 2273 Size: 1341 1012 3201 Fragments arranged by size: 3201 1341 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5554 BstBI TT'CG_AA Cuts at: 4358 4358 Size: 5554 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5554 BstXI CCAn_nnnn'nTGG Cuts at: 2287 2533 2287 Size: 246 5308 BstYI r'GATC_y Cuts at: 1972 2884 3251 3636 3653 5105 5191 5203 5549 Size: 912 367 385 17 1452 86 12 346 Cuts at: 5549 1972 Size: 1977 Fragments arranged by size: 1977 1452 912 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2335 2487 513 Size: 552 1270 152 3580 Fragments arranged by size: 3580 1270 552 152 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4815 Fragments arranged by size: 4815 683 56 BtsI GCAGTG_nn' Cuts at: 3070 3864 3884 3070 Size: 794 20 4740 Fragments arranged by size: 4740 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5554 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5519 DraI TTT'AAA Cuts at: 2858 2858 Size: 5554 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4561 809 Size: 507 3245 1802 Fragments arranged by size: 3245 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2065 2277 2622 Size: 54 1425 50 275 18 235 212 345 Cuts at: 2622 2710 3001 3890 5519 8 Size: 88 291 889 1629 43 Fragments arranged by size: 1629 1425 889 345 291 275 235 212 88 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3001 1537 Size: 275 1189 4090 Fragments arranged by size: 4090 1189 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2360 3484 1353 Size: 1007 1124 3423 Fragments arranged by size: 3423 1124 1007 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4141 4515 4661 387 Size: 1064 138 2552 374 146 1280 Fragments arranged by size: 2552 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2080 2156 2375 3617 4214 5001 5269 Size: 393 448 76 219 1242 597 787 268 Cuts at: 5269 1239 Size: 1524 Fragments arranged by size: 1524 1242 787 597 448 393 268 219 76 EcoNI CCTnn'n_nnAGG Cuts at: 2357 2357 Size: 5554 EcoO109I rG'GnC_Cy Cuts at: 2341 2341 Size: 5554 EcoRI G'AATT_C Cuts at: 2863 3241 2863 Size: 378 5176 FspI TGC'GCA Cuts at: 4060 4060 Size: 5554 HaeII r_GCGC'y Cuts at: 1678 2674 4701 1678 Size: 996 2027 2531 Fragments arranged by size: 2531 2027 996 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2132 2164 2828 2860 4195 Size: 32 135 32 560 32 664 32 1335 Cuts at: 4195 4227 4269 4301 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1335 664 560 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2898 3144 669 Size: 988 281 960 246 3079 Fragments arranged by size: 3079 988 960 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5554 HpaI GTT'AAC Cuts at: 3144 3144 Size: 5554 KpnI G_GTAC'C Cuts at: 2879 2879 Size: 5554 MfeI C'AATT_G Cuts at: 3153 3153 Size: 5554 MluI A'CGCG_T Cuts at: 5430 5430 Size: 5554 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4668 4852 1305 Size: 3363 184 2007 Fragments arranged by size: 3363 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2279 10 Size: 54 1425 343 447 3285 Fragments arranged by size: 3285 1425 447 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2285 2398 2417 3512 3871 4030 518 Size: 648 1119 113 19 1095 359 159 2042 Fragments arranged by size: 2042 1119 1095 648 359 159 113 19 MspA1I CmG'CkG Cuts at: 1606 2841 2970 3647 4795 5040 1606 Size: 1235 129 677 1148 245 2120 Fragments arranged by size: 2120 1235 1148 677 245 129 NcoI C'CATG_G Cuts at: 513 1065 2335 2487 513 Size: 552 1270 152 3580 Fragments arranged by size: 3580 1270 552 152 NdeI CA'TA_TG Cuts at: 387 387 Size: 5554 NheI G'CTAG_C Cuts at: 1052 2587 1052 Size: 1535 4019 NotI GC'GGCC_GC Cuts at: 3001 3001 Size: 5554 NspI r_CATG'y Cuts at: 1132 1229 2912 4457 1132 Size: 97 1683 1545 2229 Fragments arranged by size: 2229 1683 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5554 PciI A'CATG_T Cuts at: 4453 4453 Size: 5554 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 1108 Size: 902 4652 PmeI GTTT'AAAC Cuts at: 2858 2858 Size: 5554 PmlI CAC'GTG Cuts at: 2287 2287 Size: 5554 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3623 3655 5136 5168 3623 Size: 32 1481 32 4009 Fragments arranged by size: 4009 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2341 2341 Size: 5554 PshAI GACnn'nnGTC Cuts at: 1613 2408 1613 Size: 795 4759 PsiI TTA'TAA Cuts at: 3124 3124 Size: 5554 PstI C_TGCA'G Cuts at: 830 2125 2244 2906 830 Size: 1295 119 662 3478 Fragments arranged by size: 3478 1295 662 119 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5554 SalI G'TCGA_C Cuts at: 1936 2896 1936 Size: 960 4594 SanDI GG'GwC_CC Cuts at: 2341 2341 Size: 5554 SbfI CC_TGCA'GG Cuts at: 2906 2906 Size: 5554 ScaI AGT'ACT Cuts at: 3802 5514 3802 Size: 1712 3842 SfcI C'TryA_G Cuts at: 826 1045 2121 2240 2902 4037 4718 4909 826 Size: 219 1076 119 662 1135 681 191 1471 Fragments arranged by size: 1471 1135 1076 681 662 219 191 119 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5554 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5554 SmaI CCC'GGG Cuts at: 1872 2881 1872 Size: 1009 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2327 3658 4559 4821 820 Size: 197 926 384 1331 901 262 1553 Fragments arranged by size: 1553 1331 926 901 384 262 197 SnaBI TAC'GTA Cuts at: 493 3345 493 Size: 2852 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5554 SphI G_CATG'C Cuts at: 1132 2912 1132 Size: 1780 3774 SspI AAT'ATT Cuts at: 5 52 3478 5 Size: 47 3426 2081 Fragments arranged by size: 3426 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5554 StyI C'CwwG_G Cuts at: 513 1065 2335 2487 2993 513 Size: 552 1270 152 506 3074 Fragments arranged by size: 3074 1270 552 506 152 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2071 3581 3598 3751 3936 4381 325 Size: 1255 491 1510 17 153 185 445 1498 Fragments arranged by size: 1510 1498 1255 491 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2022 3800 5512 Size: 274 80 33 51 1443 45 1778 1712 Cuts at: 5512 96 Size: 138 Fragments arranged by size: 1778 1712 1443 274 138 80 51 45 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5514 5546 5514 Size: 32 5522 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5554 XbaI T'CTAG_A Cuts at: 2890 2890 Size: 5554 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2494 2494 Size: 5554 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5554 XmaI C'CCGG_G Cuts at: 1870 2879 1870 Size: 1009 4545 XmnI GAAnn'nnTTC Cuts at: 3683 3683 Size: 5554 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2512 3362 276 Size: 53 83 186 936 978 850 2468 Fragments arranged by size: 2468 978 936 850 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspOMI PspXI PsrI PvuII RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI