(Circular) (Six-base) MAPSORT of: pFN21AE1367.seq Check: 917 from: 1 to: 5815 pFN21AE1367 5815 bp With 182 enzymes: SgfI * August 16, 2010 14:46 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3625 278 Size: 53 83 186 936 2089 2468 Fragments arranged by size: 2468 2089 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3158 1937 Size: 1221 4594 Acc65I G'GTAC_C Cuts at: 3136 3136 Size: 5815 AclI AA'CG_TT Cuts at: 1563 3942 4315 1563 Size: 2379 373 3063 Fragments arranged by size: 3063 2379 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2212 3089 3878 5262 5530 1632 Size: 580 877 789 1384 268 1917 Fragments arranged by size: 1917 1384 877 789 580 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5618 AflIII A'CryG_T Cuts at: 1203 4714 5691 1203 Size: 3511 977 1327 Fragments arranged by size: 3511 1327 977 AgeI A'CCGG_T Cuts at: 4646 4646 Size: 5815 AhdI GACnn_n'nnGTC Cuts at: 4544 4544 Size: 5815 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5815 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5815 AlwNI CAG_nnn'CTG Cuts at: 2632 2782 3067 3558 5130 2632 Size: 150 285 491 1572 3317 Fragments arranged by size: 3317 1572 491 285 150 ApaI G_GGCC'C Cuts at: 2074 2074 Size: 5815 ApaLI G'TGCA_C Cuts at: 3872 5028 3872 Size: 1156 4659 ApoI r'AATT_y Cuts at: 1493 2901 2936 3124 3350 3502 5611 1493 Size: 1408 35 188 226 152 2109 1697 Fragments arranged by size: 2109 1697 1408 226 188 152 35 AseI AT'TA_AT Cuts at: 160 1032 4369 160 Size: 872 3337 1606 Fragments arranged by size: 3337 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5815 AvaI C'yCGr_G Cuts at: 1870 1943 2084 3140 1870 Size: 73 141 1056 4545 Fragments arranged by size: 4545 1056 141 73 BamHI G'GATC_C Cuts at: 3145 3512 3145 Size: 367 5448 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2054 2378 2656 3136 4591 Size: 325 217 627 267 324 278 480 1455 Cuts at: 4591 618 Size: 1842 Fragments arranged by size: 1842 1455 627 480 325 324 278 267 217 BanII G_rGCy'C Cuts at: 1391 2074 2300 2426 3134 1391 Size: 683 226 126 708 4072 Fragments arranged by size: 4072 708 683 226 126 BbeI G_GCGC'C Cuts at: 2058 2058 Size: 5815 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2154 928 Size: 971 255 4589 Fragments arranged by size: 4589 971 255 BbvCI CC'TCA_GC Cuts at: 2030 2030 Size: 5815 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4006 4040 1903 Size: 34 2069 34 3678 Fragments arranged by size: 3678 2069 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3707 4917 3707 Size: 1210 4605 BclI T'GATC_A Cuts at: 1548 1548 Size: 5815 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2507 2831 Size: 107 122 71 631 605 337 498 324 Cuts at: 2831 4426 136 Size: 1595 1525 Fragments arranged by size: 1595 1525 631 605 498 337 324 122 107 71 BglII A'GATC_T Cuts at: 5810 5810 Size: 5815 BlpI GC'TnA_GC Cuts at: 3232 3232 Size: 5815 Bme1580I G_kGCm'C Cuts at: 1790 2074 3876 5032 1790 Size: 284 1802 1156 2573 Fragments arranged by size: 2573 1802 1156 284 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5815 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2172 2199 2620 Size: 416 53 83 399 24 755 27 421 Cuts at: 2620 2792 3546 4504 442 Size: 172 754 958 1753 Fragments arranged by size: 1753 958 755 754 421 416 399 172 83 53 27 24 BmtI G_CTAG'C Cuts at: 1056 2120 2681 1056 Size: 1064 561 4190 Fragments arranged by size: 4190 1064 561 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2697 2697 Size: 5815 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2448 2654 2805 4475 1239 Size: 1209 206 151 1670 2579 Fragments arranged by size: 2579 1670 1209 206 151 Bpu10I CC'TnA_GC Cuts at: 2030 2030 Size: 5815 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3940 4805 5103 3940 Size: 865 298 4652 Fragments arranged by size: 4652 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2226 2426 2440 4478 882 Size: 617 727 200 14 2038 2219 Fragments arranged by size: 2219 2038 727 617 200 14 BsaAI yAC'GTr Cuts at: 493 1204 3606 493 Size: 711 2402 2702 Fragments arranged by size: 2702 2402 711 BsaBI GATnn'nnATC Cuts at: 5809 5809 Size: 5815 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2055 3622 4004 4639 Size: 53 83 186 936 522 1567 382 635 Cuts at: 4639 275 Size: 1451 Fragments arranged by size: 1567 1451 936 635 522 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4248 4646 4920 5067 5478 4248 Size: 398 274 147 411 4585 Fragments arranged by size: 4585 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2803 2833 1178 Size: 30 165 30 1400 30 4160 Fragments arranged by size: 4160 1400 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2075 2276 1186 Size: 889 201 4725 Fragments arranged by size: 4725 889 201 BseYI C'CCAG_C Cuts at: 2097 2291 5018 5514 2097 Size: 194 2727 496 2398 Fragments arranged by size: 2727 2398 496 194 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2951 3122 1163 Size: 573 1215 171 3856 Fragments arranged by size: 3856 1215 573 171 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2094 3265 4026 5054 1540 Size: 275 186 93 1171 761 1028 2301 Fragments arranged by size: 2301 1171 1028 761 275 186 93 BsiHKAI G_wGCw'C Cuts at: 3876 3961 5032 3876 Size: 85 1071 4659 Fragments arranged by size: 4659 1071 85 BsmI GAATG_Cn' Cuts at: 2527 3326 3419 2527 Size: 799 93 4923 Fragments arranged by size: 4923 799 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2074 2300 2426 3134 3876 3961 5032 Size: 399 284 226 126 708 742 85 1071 Cuts at: 5032 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 708 399 284 226 126 85 BspEI T'CCGG_A Cuts at: 5478 5478 Size: 5815 BspHI T'CATG_A Cuts at: 2242 3021 3704 5434 2242 Size: 779 683 1730 2623 Fragments arranged by size: 2623 1730 779 683 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3090 3170 844 Size: 2246 80 3489 Fragments arranged by size: 3489 2246 80 BsrBI CCG'CTC Cuts at: 1394 1823 3702 1394 Size: 429 1879 3507 Fragments arranged by size: 3507 1879 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4310 4484 65 Size: 1183 3062 174 1396 Fragments arranged by size: 3062 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2093 4459 4646 1700 Size: 110 283 2366 187 2869 Fragments arranged by size: 2869 2366 283 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5815 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5815 BssSI C'ACGA_G Cuts at: 3875 4887 3875 Size: 1012 4803 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5815 BstBI TT'CG_AA Cuts at: 4619 4619 Size: 5815 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5815 BstXI CCAn_nnnn'nTGG Cuts at: 2356 2356 Size: 5815 BstYI r'GATC_y Cuts at: 1972 3145 3512 3897 3914 5366 5452 5464 5810 Size: 1173 367 385 17 1452 86 12 346 Cuts at: 5810 1972 Size: 1977 Fragments arranged by size: 1977 1452 1173 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2997 513 Size: 552 939 993 3331 Fragments arranged by size: 3331 993 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5076 Fragments arranged by size: 5076 683 56 BtsI GCAGTG_nn' Cuts at: 2790 3331 4125 4145 2790 Size: 541 794 20 4460 Fragments arranged by size: 4460 794 541 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5815 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5780 DraI TTT'AAA Cuts at: 2374 3119 2374 Size: 745 5070 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4822 809 Size: 507 3506 1802 Fragments arranged by size: 3506 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2091 2990 3262 Size: 54 1425 50 275 18 261 899 272 Cuts at: 3262 4151 5780 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 899 889 275 272 261 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2091 3262 1537 Size: 275 279 1171 4090 Fragments arranged by size: 4090 1171 279 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2262 2487 2561 2863 2978 3745 1353 Size: 909 225 74 302 115 767 3423 Fragments arranged by size: 3423 909 767 302 225 115 74 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2027 4402 4776 4922 387 Size: 1064 138 438 2375 374 146 1280 Fragments arranged by size: 2375 1280 1064 438 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2212 2448 2654 2805 3089 3878 4475 Size: 393 580 236 206 151 284 789 597 Cuts at: 4475 5262 5530 1239 Size: 787 268 1524 Fragments arranged by size: 1524 789 787 597 580 393 284 268 236 206 151 EcoRI G'AATT_C Cuts at: 3124 3502 3124 Size: 378 5437 FspI TGC'GCA Cuts at: 4321 4321 Size: 5815 HaeII r_GCGC'y Cuts at: 1678 2030 2058 4962 1678 Size: 352 28 2904 2531 Fragments arranged by size: 2904 2531 352 28 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2480 2512 2961 2993 4456 Size: 32 135 32 908 32 449 32 1463 Cuts at: 4456 4488 4530 4562 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1463 908 449 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3111 3159 3405 669 Size: 988 281 1173 48 246 3079 Fragments arranged by size: 3079 1173 988 281 246 48 HindIII A'AGCT_T Cuts at: 748 748 Size: 5815 HpaI GTT'AAC Cuts at: 3111 3405 3111 Size: 294 5521 KasI G'GCGC_C Cuts at: 2054 2054 Size: 5815 KpnI G_GTAC'C Cuts at: 3140 3140 Size: 5815 MfeI C'AATT_G Cuts at: 3414 3414 Size: 5815 MluI A'CGCG_T Cuts at: 5691 5691 Size: 5815 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2992 4929 5113 1305 Size: 1687 1937 184 2007 Fragments arranged by size: 2007 1937 1687 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3993 Fragments arranged by size: 3993 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3773 4132 4291 518 Size: 648 2607 359 159 2042 Fragments arranged by size: 2607 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3106 3231 3908 5056 5301 1606 Size: 1500 125 677 1148 245 2120 Fragments arranged by size: 2120 1500 1148 677 245 125 NaeI GCC'GGC Cuts at: 2095 2095 Size: 5815 NarI GG'CG_CC Cuts at: 2055 2055 Size: 5815 NcoI C'CATG_G Cuts at: 513 1065 2004 2997 513 Size: 552 939 993 3331 Fragments arranged by size: 3331 993 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5815 NgoMIV G'CCGG_C Cuts at: 2093 2093 Size: 5815 NheI G'CTAG_C Cuts at: 1052 2116 2677 1052 Size: 1064 561 4190 Fragments arranged by size: 4190 1064 561 NotI GC'GGCC_GC Cuts at: 3262 3262 Size: 5815 NspI r_CATG'y Cuts at: 1132 1229 3173 4718 1132 Size: 97 1944 1545 2229 Fragments arranged by size: 2229 1944 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5815 PciI A'CATG_T Cuts at: 4714 4714 Size: 5815 PflMI CCAn_nnn'nTGG Cuts at: 1108 2632 1108 Size: 1524 4291 PmeI GTTT'AAAC Cuts at: 3119 3119 Size: 5815 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3884 3916 5397 5429 3884 Size: 32 1481 32 4270 Fragments arranged by size: 4270 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5815 PsiI TTA'TAA Cuts at: 3385 3385 Size: 5815 PspOMI G'GGCC_C Cuts at: 2070 2070 Size: 5815 PstI C_TGCA'G Cuts at: 830 3167 830 Size: 2337 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5815 SalI G'TCGA_C Cuts at: 1936 3157 1936 Size: 1221 4594 SapI GCTCTTCn'nnn_ Cuts at: 2863 2863 Size: 5815 SbfI CC_TGCA'GG Cuts at: 3167 3167 Size: 5815 ScaI AGT'ACT Cuts at: 4063 5775 4063 Size: 1712 4103 SfcI C'TryA_G Cuts at: 826 1045 2397 3163 4298 4979 5170 826 Size: 219 1352 766 1135 681 191 1471 Fragments arranged by size: 1471 1352 1135 766 681 219 191 SfoI GGC'GCC Cuts at: 2056 2056 Size: 5815 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5815 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5815 SmaI CCC'GGG Cuts at: 1872 3142 1872 Size: 1270 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2084 3919 4820 5082 820 Size: 197 926 141 1835 901 262 1553 Fragments arranged by size: 1835 1553 926 901 262 197 141 SnaBI TAC'GTA Cuts at: 493 3606 493 Size: 3113 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5815 SphI G_CATG'C Cuts at: 1132 3173 1132 Size: 2041 3774 SspI AAT'ATT Cuts at: 5 52 3739 5 Size: 47 3687 2081 Fragments arranged by size: 3687 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5815 StyI C'CwwG_G Cuts at: 513 1065 2004 2473 2997 3254 513 Size: 552 939 469 524 257 3074 Fragments arranged by size: 3074 939 552 524 469 257 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3842 3859 4012 4197 4642 325 Size: 1255 2262 17 153 185 445 1498 Fragments arranged by size: 2262 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4061 5773 96 Size: 274 80 33 51 1443 2084 1712 138 Fragments arranged by size: 2084 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5775 5807 5775 Size: 32 5783 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5815 XbaI T'CTAG_A Cuts at: 3151 3151 Size: 5815 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2356 2356 Size: 5815 XhoI C'TCGA_G Cuts at: 1943 2084 1943 Size: 141 5674 XmaI C'CCGG_G Cuts at: 1870 3140 1870 Size: 1270 4545 XmnI GAAnn'nnTTC Cuts at: 3944 3944 Size: 5815 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3623 276 Size: 53 83 186 936 2089 2468 Fragments arranged by size: 2468 2089 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI