################################## # NO CUT ENZYMES ( 127 enzymes) # ################################## AatII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AspI AvaI AviII AvrII BbeI BbrPI BcgI BclI BfaI BfrI BglI BglII BlnI BsaAI BsaBI BsaHI BseRI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstXI BstZ17I CfoI ClaI CpoI DraI DraII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO109I EcoRI EcoRV FauI FbaI FseI FspI HaeII HhaI Hin1I HinP1I HindIII HpaI KasI MaeI MamI MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### Acc65I 293 GGTACC G^GTACC AccI 1166 GTCGAC GT^[AC][GT]AC AccII 9 CGCG CG^CG AocI 1371 CCTCAGG CC^T[ACGT]AGG ApaI 494 GGGCCC GGGCC^C Asp718I 293 GGTACC G^GTACC AvaII 5 GGACC G^G[AT]CC BalI 1360 TGGCCA TGG^CCA BamHI 1465 GGATCC G^GATCC BanI 293 GGTACC G^G[CT][GA]CC BbvCI 262 GCTGAGG CCTCAGC(-5/-2) BciVI 1389 GGATAC GTATCC(6/5) BcnI 820 CCGGG CC^[GC]GG BsaI 386 GAGACC GGTCTC(1/5) BspMI 1455 GCAGGT ACCTGC(4/8) BsrFI 159 GCCGGT [GA]^CCGG[CT] BstEII 621 GGTCACC G^GT[ACGT]ACC BstPI 621 GGTCACC G^GT[ACGT]ACC BstUI 9 CGCG CG^CG Bsu36I 1371 CCTCAGG CC^T[ACGT]AGG Cfr10I 159 GCCGGT [GA]^CCGG[CT] EaeI 1360 TGGCCA [CT]^GGCC[GA] Eco81I 1371 CCTCAGG CC^T[ACGT]AGG EcoNI 327 CCTGAAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 621 GGTCACC G^GT[ACGT]ACC EcoT14I 933 CCAAGG C^C[AT][AT]GG EcoT22I 207 ATGCAT ATGCA^T HgaI 1417 GCGTC GACGC(5/10) HincII 1166 GTCGAC GT[CT]^[GA]AC HindII 1166 GTCGAC GT[CT]^[GA]AC HinfI 808 GACTC G^A[ACGT]TC KpnI 293 GGTACC GGTAC^C KspI 8 CCGCGG CCGC^GG MfeI 1268 CAATTG C^AATTG MluNI 1360 TGGCCA TGG^CCA MscI 1360 TGGCCA TGG^CCA MseI 1090 TTAA T^TAA MunI 1268 CAATTG C^AATTG MvnI 9 CGCG CG^CG NciI 820 CCGGG CC^[GC]GG NsiI 207 ATGCAT ATGCA^T PleI 808 GACTC GAGTC(4/5) SacII 8 CCGCGG CCGC^GG SalI 1166 GTCGAC G^TCGAC SapI 961 GAAGAGC GCTCTTC(1/4) SexAI 836 ACCAGGT A^CC[AT]GGT StuI 137 AGGCCT AGG^CCT StyI 933 CCAAGG C^C[AT][AT]GG TaqI 1167 TCGA T^CGA Tru9I 1090 TTAA T^TAA TthHB8I 1167 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AccIII 811 TCCGGA T^CCGGA 1448 TCCGGA AspHI 1028 GAGCAC G[AT]GC[AT]^C 1440 GAGCAC BanII 494 GGGCCC G[GA]GC[CT]^C 964 GAGCCC BbsI 559 GAAGAC GAAGAC(2/6) 481 GTCTTC BlpI 22 GCTCAGC GC^T[ACGT]AGC 1335 GCTAAGC Bpu1102I 22 GCTCAGC GC^T[ACGT]AGC 1335 GCTAAGC BpuAI 559 GAAGAC GAAGAC(2/6) 481 GTCTTC BseAI 811 TCCGGA T^CCGGA 1448 TCCGGA BsiHKAI 1028 GAGCAC G[AT]GC[AT]^C 1440 GAGCAC BspEI 811 TCCGGA T^CCGGA 1448 TCCGGA BstYI 1288 AGATCC [GA]^GATC[CT] 1465 GGATCC CelII 22 GCTCAGC GC^T[ACGT]AGC 1335 GCTAAGC DraIII 419 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG 835 CACCAGGTG DsaI 8 CCGCGG C^C[GA][CT]GG 867 CCGTGG EarI 1312 CTCTTC CTCTTC(1/4) 961 GAAGAG Ksp632I 1312 CTCTTC CTCTTC(1/4) 961 GAAGAG MaeII 764 ACGT A^CGT 920 ACGT MflI 1288 AGATCC [GA]^GATC[CT] 1465 GGATCC MroI 811 TCCGGA T^CCGGA 1448 TCCGGA PflMI 862 CCAGGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1190 CCATCAGCTGG PstI 608 CTGCAG CTGCA^G 1099 CTGCAG SmlI 451 CTTGAG C^T[CT][GA]AG 595 CTTGAG Van91I 862 CCAGGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1190 CCATCAGCTGG XhoII 1288 AGATCC [GA]^GATC[CT] 1465 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 56 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI BbvI BmyI BpmI BsaJI BsaWI BsgI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HapII HpaII HphI ItaI MaeIII MboI MboII MnlI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TseI Tsp45I Tsp509I TspRI XcmI