################################## # NO CUT ENZYMES ( 130 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbrPI BbvCI BciVI BclI BfaI BfrI BlnI BsaAI BsaBI BsaWI BseAI BseRI BsgI BsiWI BsmBI BsmFI BsmI Bsp1407I BspDI BspEI BspHI BsrBI BsrDI BsrFI BsrGI Bst1107I BstAPI BstBI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraIII DrdI Eam1105I Eco47III Eco81I EcoNI EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI KspI MaeI MaeII MamI MfeI MluI MroI MseI MslI MunI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tsp509I Tth111I Van91I XbaI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AccI 772 GTCTAC GT^[AC][GT]AC AflIII 794 ACATGT A^C[GA][CT]GT AlwI 803 GGATC GGATC(4/5) BalI 428 TGGCCA TGG^CCA BbsI 607 GTCTTC GAAGAC(2/6) BcgI 53 CGAAGCTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 88 GCCGCAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 782 AGATCT A^GATCT BlpI 487 GCTCAGC GC^T[ACGT]AGC Bpu1102I 487 GCTCAGC GC^T[ACGT]AGC BpuAI 607 GTCTTC GAAGAC(2/6) BspLU11I 794 ACATGT A^CATGT BssSI 729 CTCGTG CACGAG(-5/-1) BstEII 624 GGTCACC G^GT[ACGT]ACC BstPI 624 GGTCACC G^GT[ACGT]ACC BstXI 507 CCAGGTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 782 AGATCT [GA]^GATC[CT] CelII 487 GCTCAGC GC^T[ACGT]AGC DsaI 527 CCACGG C^C[GA][CT]GG EarI 187 CTCTTC CTCTTC(1/4) EcoO65I 624 GGTCACC G^GT[ACGT]ACC HgaI 455 GCGTC GACGC(5/10) HphI 626 TCACC GGTGA(8/7) Ksp632I 187 CTCTTC CTCTTC(1/4) MflI 782 AGATCT [GA]^GATC[CT] MluNI 428 TGGCCA TGG^CCA MscI 428 TGGCCA TGG^CCA NspI 794 ACATGT [GA]CATG^[CT] PaeR7I 281 CTCGAG C^TCGAG PpuMI 665 AGGACCC [GA]G^G[AT]CC[CT] PstI 466 CTGCAG CTGCA^G PvuII 767 CAGCTG CAG^CTG SacI 5 GAGCTC GAGCT^C SapI 186 GCTCTTC GCTCTTC(1/4) SfcI 466 CTGCAG C^T[GA][CT]AG SmaI 41 CCCGGG CCC^GGG SmlI 281 CTCGAG C^T[CT][GA]AG TaqI 282 TCGA T^CGA TthHB8I 282 TCGA T^CGA XcmI 675 CCAGAGTGACTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 281 CTCGAG C^TCGAG XhoII 782 AGATCT [GA]^GATC[CT] XmaCI 41 CCCGGG C^CCGGG XmaI 41 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AcyI 37 GGCGCC G[GA]^CG[CT]C 145 GGCGCC AfaI 360 GTAC GT^AC 549 GTAC ApaI 448 GGGCCC GGGCC^C 461 GGGCCC AvaI 41 CCCGGG C^[CT]CG[GA]G 281 CTCGAG BbeI 37 GGCGCC GGCGC^C 145 GGCGCC BpmI 570 CTCCAG CTGGAG(16/14) 686 CTGGAG BsaHI 37 GGCGCC G[GA]^CG[CT]C 145 GGCGCC BsiEI 248 CGGCCG CG[GA][CT]^CG 530 CGGCCG BsoBI 41 CCCGGG C^[CT]CG[GA]G 281 CTCGAG BsrI 651 ACTGG ACTGG(1/-1) 501 CCAGT BssHII 155 GCGCGC G^CGCGC 157 GCGCGC EagI 248 CGGCCG C^GGCCG 530 CGGCCG EclXI 248 CGGCCG C^GGCCG 530 CGGCCG Eco52I 248 CGGCCG C^GGCCG 530 CGGCCG EcoT14I 388 CCAAGG C^C[AT][AT]GG 717 CCAAGG FauI 452 CCCGC CCCGC(4/6) 214 GCGGG Hin1I 37 GGCGCC G[GA]^CG[CT]C 145 GGCGCC HinfI 336 GACTC G^A[ACGT]TC 682 GACTC KasI 37 GGCGCC G^GCGCC 145 GGCGCC NarI 37 GGCGCC GG^CGCC 145 GGCGCC PleI 336 GACTC GAGTC(4/5) 682 GACTC RsaI 360 GTAC GT^AC 549 GTAC SfoI 37 GGCGCC GGC^GCC 145 GGCGCC StyI 388 CCAAGG C^C[AT][AT]GG 717 CCAAGG ######################################### # 3 OR MORE CUT ENZYMES ( 60 enzymes) # ######################################### AccII AciI AluI AspHI AvaII BanI BanII BbvI BcnI BmyI BsaI BsaJI BsiHKAI BsiYI BslI BsmAI Bsp1286I BspMI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EcoO109I EcoRII Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HpaII ItaI MaeIII MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI TseI Tsp45I TspRI