(Circular) (Six-base) MAPSORT of: pFN21AE6207.seq Check: 6586 from: 1 to: 5533 pFN21AE6207 5533 bp With 182 enzymes: SgfI * March 18, 2015 14:49 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2313 2313 Size: 5533 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3343 278 Size: 53 83 186 936 1807 2468 Fragments arranged by size: 2468 1807 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2778 2876 1937 Size: 841 98 4594 Fragments arranged by size: 4594 841 98 Acc65I G'GTAC_C Cuts at: 2854 2854 Size: 5533 AclI AA'CG_TT Cuts at: 1563 2832 3660 4033 1563 Size: 1269 828 373 3063 Fragments arranged by size: 3063 1269 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2300 3596 4980 5248 1632 Size: 668 1296 1384 268 1917 Fragments arranged by size: 1917 1384 1296 668 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5336 AflIII A'CryG_T Cuts at: 1203 2799 4432 5409 1203 Size: 1596 1633 977 1327 Fragments arranged by size: 1633 1596 1327 977 AgeI A'CCGG_T Cuts at: 4364 4364 Size: 5533 AhdI GACnn_n'nnGTC Cuts at: 4262 4262 Size: 5533 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5533 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5533 AlwNI CAG_nnn'CTG Cuts at: 3276 4848 3276 Size: 1572 3961 ApaI G_GGCC'C Cuts at: 2457 2470 2457 Size: 13 5520 ApaLI G'TGCA_C Cuts at: 3590 4746 3590 Size: 1156 4377 ApoI r'AATT_y Cuts at: 1493 2842 3068 3220 5329 1493 Size: 1349 226 152 2109 1697 Fragments arranged by size: 2109 1697 1349 226 152 AseI AT'TA_AT Cuts at: 160 1032 4087 160 Size: 872 3055 1606 Fragments arranged by size: 3055 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5533 AvaI C'yCGr_G Cuts at: 1870 1943 2046 2286 2858 1870 Size: 73 103 240 572 4545 Fragments arranged by size: 4545 572 240 103 73 BamHI G'GATC_C Cuts at: 2863 3230 2863 Size: 367 5166 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2042 2150 2528 2854 4309 Size: 325 217 627 255 108 378 326 1455 Cuts at: 4309 618 Size: 1842 Fragments arranged by size: 1842 1455 627 378 326 325 255 217 108 BanII G_rGCy'C Cuts at: 1391 2014 2053 2287 2378 2457 2470 2597 2852 Size: 623 39 234 91 79 13 127 255 Cuts at: 2852 1391 Size: 4072 Fragments arranged by size: 4072 623 255 234 127 91 79 39 13 BbeI G_GCGC'C Cuts at: 2046 2154 2046 Size: 108 5425 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2605 928 Size: 971 706 3856 Fragments arranged by size: 3856 971 706 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2047 2081 3724 3758 1903 Size: 34 110 34 1643 34 3678 Fragments arranged by size: 3678 1643 110 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3425 4635 3425 Size: 1210 4323 BclI T'GATC_A Cuts at: 1548 1548 Size: 5533 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2099 4144 136 Size: 107 122 71 631 605 427 2045 1525 Fragments arranged by size: 2045 1525 631 605 427 122 107 71 BglII A'GATC_T Cuts at: 2787 5528 2787 Size: 2741 2792 BlpI GC'TnA_GC Cuts at: 2493 2950 2493 Size: 457 5076 Bme1580I G_kGCm'C Cuts at: 1790 2457 2470 2531 3594 4750 1790 Size: 667 13 61 1063 1156 2573 Fragments arranged by size: 2573 1156 1063 667 61 13 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5533 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3264 4222 442 Size: 416 53 83 399 24 1847 958 1753 Fragments arranged by size: 1847 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5533 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2560 2712 4193 1239 Size: 1321 152 1481 2579 Fragments arranged by size: 2579 1481 1321 152 Bpu10I CC'TnA_GC Cuts at: 2377 2591 2377 Size: 214 5319 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3658 4523 4821 3658 Size: 865 298 4370 Fragments arranged by size: 4370 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2191 2496 2708 4196 882 Size: 617 692 305 212 1488 2219 Fragments arranged by size: 2219 1488 692 617 305 212 BsaAI yAC'GTr Cuts at: 493 1204 3324 493 Size: 711 2120 2702 Fragments arranged by size: 2702 2120 711 BsaBI GATnn'nnATC Cuts at: 5527 5527 Size: 5533 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2043 2151 3340 3722 Size: 53 83 186 936 510 108 1189 382 Cuts at: 3722 4357 275 Size: 635 1451 Fragments arranged by size: 1451 1189 936 635 510 382 186 108 83 53 BsaWI w'CCGG_w Cuts at: 3966 4364 4638 4785 5196 3966 Size: 398 274 147 411 4303 Fragments arranged by size: 4303 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5308 Fragments arranged by size: 5308 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5533 BseYI C'CCAG_C Cuts at: 2523 2763 4736 5232 2523 Size: 240 1973 496 2824 Fragments arranged by size: 2824 1973 496 240 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4960 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2256 2538 2983 3744 4772 1540 Size: 275 186 255 282 445 761 1028 2301 Fragments arranged by size: 2301 1028 761 445 282 275 255 186 BsiHKAI G_wGCw'C Cuts at: 2014 2412 2494 3594 3679 4750 2014 Size: 398 82 1100 85 1071 2797 Fragments arranged by size: 2797 1100 1071 398 85 82 BsmI GAATG_Cn' Cuts at: 3044 3137 3044 Size: 93 5440 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2014 2053 2287 2378 2412 2457 2470 Size: 399 224 39 234 91 34 45 13 Cuts at: 2470 2494 2531 2597 2852 3594 3679 4750 1391 Size: 24 37 66 255 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 255 234 224 91 85 66 45 39 37 34 24 13 BspEI T'CCGG_A Cuts at: 5196 5196 Size: 5533 BspHI T'CATG_A Cuts at: 3422 5152 3422 Size: 1730 3803 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2173 2313 2579 2888 844 Size: 1329 140 266 309 3489 Fragments arranged by size: 3489 1329 309 266 140 BsrBI CCG'CTC Cuts at: 1394 1823 3420 1394 Size: 429 1597 3507 Fragments arranged by size: 3507 1597 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4028 4202 65 Size: 1183 2780 174 1396 Fragments arranged by size: 2780 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4177 4364 1700 Size: 110 2367 187 2869 Fragments arranged by size: 2869 2367 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5533 BssHII G'CGCG_C Cuts at: 1925 2160 2162 1925 Size: 235 2 5296 Fragments arranged by size: 5296 235 2 BssSI C'ACGA_G Cuts at: 2734 3593 4605 2734 Size: 859 1012 3662 Fragments arranged by size: 3662 1012 859 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5533 BstBI TT'CG_AA Cuts at: 4337 4337 Size: 5533 BstEII G'GTnAC_C Cuts at: 1184 2629 1184 Size: 1445 4088 BstXI CCAn_nnnn'nTGG Cuts at: 2519 2519 Size: 5533 BstYI r'GATC_y Cuts at: 1972 2787 2863 3230 3615 3632 5084 5170 5182 Size: 815 76 367 385 17 1452 86 12 Cuts at: 5182 5528 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 815 385 367 346 86 76 17 12 BtgI C'CryG_G Cuts at: 513 1065 2532 513 Size: 552 1467 3514 Fragments arranged by size: 3514 1467 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4794 Fragments arranged by size: 4794 683 56 BtsI GCAGTG_nn' Cuts at: 2212 2260 3049 3843 3863 2212 Size: 48 789 794 20 3882 Fragments arranged by size: 3882 794 789 48 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5533 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5498 DraI TTT'AAA Cuts at: 2837 2837 Size: 5533 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4540 809 Size: 507 3224 1802 Fragments arranged by size: 3224 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2253 2433 2535 Size: 54 1425 50 275 18 423 180 102 Cuts at: 2535 2980 3869 5498 8 Size: 445 889 1629 43 Fragments arranged by size: 1629 1425 889 445 423 275 180 102 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2253 2535 2980 1537 Size: 275 441 282 445 4090 Fragments arranged by size: 4090 445 441 282 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2198 3463 1353 Size: 845 1265 3423 Fragments arranged by size: 3423 1265 845 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2020 2321 4120 4494 4640 387 Size: 1064 138 431 301 1799 374 146 1280 Fragments arranged by size: 1799 1280 1064 431 374 301 146 138 EcoICRI GAG'CTC Cuts at: 2012 2012 Size: 5533 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2300 2560 2712 3596 4193 4980 5248 Size: 393 668 260 152 884 597 787 268 Cuts at: 5248 1239 Size: 1524 Fragments arranged by size: 1524 884 787 668 597 393 268 260 152 EcoO109I rG'GnC_Cy Cuts at: 2381 2453 2454 2467 2563 2671 2696 2381 Size: 72 1 13 96 108 25 5218 Fragments arranged by size: 5218 108 96 72 25 13 1 EcoRI G'AATT_C Cuts at: 2842 3220 2842 Size: 378 5155 FspI TGC'GCA Cuts at: 4039 4039 Size: 5533 HaeII r_GCGC'y Cuts at: 1678 2046 2108 2154 4680 1678 Size: 368 62 46 2526 2531 Fragments arranged by size: 2531 2526 368 62 46 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4174 4206 4248 4280 1373 Size: 32 135 32 2602 32 42 32 2626 Fragments arranged by size: 2626 2602 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2877 3123 669 Size: 988 281 939 246 3079 Fragments arranged by size: 3079 988 939 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5533 HpaI GTT'AAC Cuts at: 3123 3123 Size: 5533 KasI G'GCGC_C Cuts at: 2042 2150 2042 Size: 108 5425 KpnI G_GTAC'C Cuts at: 2858 2858 Size: 5533 MfeI C'AATT_G Cuts at: 3132 3132 Size: 5533 MluI A'CGCG_T Cuts at: 5409 5409 Size: 5533 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2853 4647 4831 1305 Size: 1548 1794 184 2007 Fragments arranged by size: 2007 1794 1548 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2435 10 Size: 54 1425 343 603 3108 Fragments arranged by size: 3108 1425 603 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3491 3850 4009 518 Size: 648 2325 359 159 2042 Fragments arranged by size: 2325 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2071 2487 2774 2949 3626 4774 5019 1606 Size: 465 416 287 175 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 465 416 287 245 175 NarI GG'CG_CC Cuts at: 2043 2151 2043 Size: 108 5425 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4981 NdeI CA'TA_TG Cuts at: 387 387 Size: 5533 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5533 NotI GC'GGCC_GC Cuts at: 2980 2980 Size: 5533 NspI r_CATG'y Cuts at: 1132 1229 2803 2891 4436 1132 Size: 97 1574 88 1545 2229 Fragments arranged by size: 2229 1574 1545 97 88 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5533 PciI A'CATG_T Cuts at: 2799 4432 2799 Size: 1633 3900 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5533 PfoI T'CCnGG_A Cuts at: 2804 2804 Size: 5533 PmeI GTTT'AAAC Cuts at: 2837 2837 Size: 5533 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3602 3634 5115 5147 3602 Size: 32 1481 32 3988 Fragments arranged by size: 3988 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2671 2671 Size: 5533 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5533 PsiI TTA'TAA Cuts at: 3103 3103 Size: 5533 PspOMI G'GGCC_C Cuts at: 2453 2466 2453 Size: 13 5520 PspXI vC'TCGA_Gb Cuts at: 2286 2286 Size: 5533 PstI C_TGCA'G Cuts at: 830 2475 2885 830 Size: 1645 410 3478 Fragments arranged by size: 3478 1645 410 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5533 PvuII CAG'CTG Cuts at: 2774 2774 Size: 5533 SacI G_AGCT'C Cuts at: 2014 2014 Size: 5533 SalI G'TCGA_C Cuts at: 1936 2875 1936 Size: 939 4594 SapI GCTCTTCn'nnn_ Cuts at: 2198 2198 Size: 5533 SbfI CC_TGCA'GG Cuts at: 2885 2885 Size: 5533 ScaI AGT'ACT Cuts at: 3781 5493 3781 Size: 1712 3821 SfcI C'TryA_G Cuts at: 826 1045 2471 2881 4016 4697 4888 826 Size: 219 1426 410 1135 681 191 1471 Fragments arranged by size: 1471 1426 1135 681 410 219 191 SfoI GGC'GCC Cuts at: 2044 2152 2044 Size: 108 5425 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5533 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5533 SmaI CCC'GGG Cuts at: 1872 2048 2860 1872 Size: 176 812 4545 Fragments arranged by size: 4545 812 176 SmlI C'TyrA_G Cuts at: 820 1017 1943 2286 3637 4538 4800 820 Size: 197 926 343 1351 901 262 1553 Fragments arranged by size: 1553 1351 926 901 343 262 197 SnaBI TAC'GTA Cuts at: 493 3324 493 Size: 2831 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5533 SphI G_CATG'C Cuts at: 1132 2891 1132 Size: 1759 3774 SrfI GCCC'GGGC Cuts at: 2048 2048 Size: 5533 SspI AAT'ATT Cuts at: 5 52 3457 5 Size: 47 3405 2081 Fragments arranged by size: 3405 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5533 StyI C'CwwG_G Cuts at: 513 1065 2393 2722 2972 513 Size: 552 1328 329 250 3074 Fragments arranged by size: 3074 1328 552 329 250 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2233 2798 3560 3577 3730 3915 4360 Size: 1255 653 565 762 17 153 185 445 Cuts at: 4360 325 Size: 1498 Fragments arranged by size: 1498 1255 762 653 565 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2553 3779 5491 Size: 274 80 33 51 1443 576 1226 1712 Cuts at: 5491 96 Size: 138 Fragments arranged by size: 1712 1443 1226 576 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5493 5525 5493 Size: 32 5501 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5533 XbaI T'CTAG_A Cuts at: 2869 2869 Size: 5533 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2687 2687 Size: 5533 XhoI C'TCGA_G Cuts at: 1943 2286 1943 Size: 343 5190 XmaI C'CCGG_G Cuts at: 1870 2046 2858 1870 Size: 176 812 4545 Fragments arranged by size: 4545 812 176 XmnI GAAnn'nnTTC Cuts at: 3662 3662 Size: 5533 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3341 276 Size: 53 83 186 936 1807 2468 Fragments arranged by size: 2468 1807 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PsrI RsrII SacII SanDI SexAI SfiI SwaI