################################## # NO CUT ENZYMES ( 129 enzymes) # ################################## AatII AccI AccII AclI AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI AspEI AspHI AviII AvrII BalI BamHI BbeI BbrPI BbvCI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaHI BsaI BsiEI BsiHKAI BsiWI BsmI BspDI BspHI BspLU11I BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HpaI KasI KspI MluI MluNI MscI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PflMI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SbfI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Van91I XmaCI XmaI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### Acc65I 631 GGTACC G^GTACC AccIII 677 TCCGGA T^CCGGA AflIII 1476 ACGTGT A^C[GA][CT]GT Asp718I 631 GGTACC G^GTACC AspI 1612 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 748 CTCGAG C^[CT]CG[GA]G BanI 631 GGTACC G^G[CT][GA]CC BanII 201 GGGCTC G[GA]GC[CT]^C BbsI 1099 GTCTTC GAAGAC(2/6) BcgI 368 GCAGCACCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BpuAI 1099 GTCTTC GAAGAC(2/6) BsaBI 458 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 677 TCCGGA [AT]^CCGG[AT] BseAI 677 TCCGGA T^CCGGA BseRI 1563 GAGGAG GAGGAG(10/8) BsmFI 1073 GGGAC GGGAC(10/14) BsoBI 748 CTCGAG C^[CT]CG[GA]G Bsp1407I 380 TGTACA T^GTACA BspEI 677 TCCGGA T^CCGGA BsrGI 380 TGTACA T^GTACA BstAPI 1518 GCAGGAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 1281 TTCGAA TT^CGAA DraII 1642 AGGACCC [GA]G^G[ACGT]CC[CT] DsaI 206 CCACGG C^C[GA][CT]GG EaeI 353 TGGCCG [CT]^GGCC[GA] EcoO109I 1642 AGGACCC [GA]G^G[ACGT]CC[CT] HapII 678 CCGG C^CGG HincII 862 GTCAAC GT[CT]^[GA]AC HindII 862 GTCAAC GT[CT]^[GA]AC HindIII 736 AAGCTT A^AGCTT HpaII 678 CCGG C^CGG KpnI 631 GGTACC GGTAC^C MamI 458 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 125 CAATTG C^AATTG MroI 677 TCCGGA T^CCGGA MslI 372 CACCTTCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 678 CCGG C^CGG MunI 125 CAATTG C^AATTG NspI 1762 GCATGT [GA]CATG^[CT] NspV 1281 TTCGAA TT^CGAA PaeR7I 748 CTCGAG C^TCGAG PflFI 1612 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 1642 AGGACCC [GA]G^G[AT]CC[CT] PshAI 894 GACCTACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SapI 766 GAAGAGC GCTCTTC(1/4) ScaI 1337 AGTACT AGT^ACT SfuI 1281 TTCGAA TT^CGAA SmlI 748 CTCGAG C^T[CT][GA]AG SspBI 380 TGTACA T^GTACA Tth111I 1612 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 526 CCAGACTTTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 748 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### BciVI 1361 GGATAC GTATCC(6/5) 674 GTATCC BmyI 201 GGGCTC G[AGT]GC[ACT]^C 247 GTGCCC BsmBI 1300 CGTCTC CGTCTC(1/5) 1749 GAGACG Bsp1286I 201 GGGCTC G[AGT]GC[ACT]^C 247 GTGCCC BspMI 659 ACCTGC ACCTGC(4/8) 1064 ACCTGC BsrI 635 CCAGT ACTGG(1/-1) 1620 CCAGT EarI 766 GAAGAG CTCTTC(1/4) 1372 GAAGAG EcoT14I 953 CCTTGG C^C[AT][AT]GG 1400 CCAAGG FauI 1173 GCGGG CCCGC(4/6) 1270 GCGGG Ksp632I 766 GAAGAG CTCTTC(1/4) 1372 GAAGAG MaeII 899 ACGT A^CGT 1476 ACGT MaeIII 320 GTGAC ^GT[ACGT]AC 433 GTGAC MseI 656 TTAA T^TAA 1655 TTAA PstI 977 CTGCAG CTGCA^G 1381 CTGCAG SfcI 977 CTGCAG C^T[GA][CT]AG 1381 CTGCAG StyI 953 CCTTGG C^C[AT][AT]GG 1400 CCAAGG TaqI 749 TCGA T^CGA 1282 TCGA Tru9I 656 TTAA T^TAA 1655 TTAA Tsp45I 320 GTGAC ^GT[GC]AC 433 GTGAC TthHB8I 749 TCGA T^CGA 1282 TCGA XbaI 876 TCTAGA T^CTAGA 1251 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 56 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI Asp700I AvaII BbvI BfaI BpmI BsaJI BsgI BsiYI BslI BsmAI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI MaeI MboI MboII MflI MnlI MspA1I MvaI MwoI NdeII NlaIII NlaIV PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI TfiI TseI Tsp509I TspRI XhoII XmnI