(Circular) (Six-base) MAPSORT of: pFN21AE4530.seq Check: 6350 from: 1 to: 6481 pFN21AE4530 6481 bp With 182 enzymes: SgfI * March 28, 2012 14:09 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4291 278 Size: 53 83 186 936 2755 2468 Fragments arranged by size: 2755 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3824 1937 Size: 1887 4594 Acc65I G'GTAC_C Cuts at: 2636 3802 2636 Size: 1166 5315 AclI AA'CG_TT Cuts at: 1563 4608 4981 1563 Size: 3045 373 3063 Fragments arranged by size: 3063 3045 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2108 2642 2648 3221 3353 3396 3492 3650 Size: 476 534 6 573 132 43 96 158 Cuts at: 3650 4544 5928 6196 1632 Size: 894 1384 268 1917 Fragments arranged by size: 1917 1384 894 573 534 476 268 158 132 96 43 6 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6284 AflIII A'CryG_T Cuts at: 1203 3481 5380 6357 1203 Size: 2278 1899 977 1327 Fragments arranged by size: 2278 1899 1327 977 AgeI A'CCGG_T Cuts at: 5312 5312 Size: 6481 AhdI GACnn_n'nnGTC Cuts at: 5210 5210 Size: 6481 AleI CACnn'nnGTG Cuts at: 1166 2381 1166 Size: 1215 5266 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6481 AlwNI CAG_nnn'CTG Cuts at: 2676 3061 3631 4224 5796 2676 Size: 385 570 593 1572 3361 Fragments arranged by size: 3361 1572 593 570 385 ApaLI G'TGCA_C Cuts at: 4538 5694 4538 Size: 1156 5325 ApoI r'AATT_y Cuts at: 1493 2687 2786 3279 3790 4016 4168 6277 1493 Size: 1194 99 493 511 226 152 2109 1697 Fragments arranged by size: 2109 1697 1194 511 493 226 152 99 AseI AT'TA_AT Cuts at: 160 1032 5035 160 Size: 872 4003 1606 Fragments arranged by size: 4003 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6481 AvaI C'yCGr_G Cuts at: 1870 1943 2753 3806 1870 Size: 73 810 1053 4545 Fragments arranged by size: 4545 1053 810 73 BamHI G'GATC_C Cuts at: 3811 4178 3811 Size: 367 6114 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2636 3802 5257 618 Size: 325 217 627 849 1166 1455 1842 Fragments arranged by size: 1842 1455 1166 849 627 325 217 BanII G_rGCy'C Cuts at: 1391 2210 3800 1391 Size: 819 1590 4072 Fragments arranged by size: 4072 1590 819 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3097 928 Size: 971 1198 4312 Fragments arranged by size: 4312 1198 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2362 2396 4672 4706 1903 Size: 34 425 34 2276 34 3678 Fragments arranged by size: 3678 2276 425 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2690 3360 4373 5583 2690 Size: 670 1013 1210 3588 Fragments arranged by size: 3588 1210 1013 670 BclI T'GATC_A Cuts at: 1548 1548 Size: 6481 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5092 136 Size: 107 122 71 631 605 3420 1525 Fragments arranged by size: 3420 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6476 6476 Size: 6481 BlpI GC'TnA_GC Cuts at: 3898 3898 Size: 6481 Bme1580I G_kGCm'C Cuts at: 1790 2256 4542 5698 1790 Size: 466 2286 1156 2573 Fragments arranged by size: 2573 2286 1156 466 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6481 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4212 5170 442 Size: 416 53 83 399 24 2795 958 1753 Fragments arranged by size: 2795 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6481 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3038 3416 3725 5141 1239 Size: 1799 378 309 1416 2579 Fragments arranged by size: 2579 1799 1416 378 309 Bpu10I CC'TnA_GC Cuts at: 2369 3226 2369 Size: 857 5624 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4606 5471 5769 4606 Size: 865 298 5318 Fragments arranged by size: 5318 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5144 882 Size: 617 3645 2219 Fragments arranged by size: 3645 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4272 493 Size: 711 3068 2702 Fragments arranged by size: 3068 2702 711 BsaBI GATnn'nnATC Cuts at: 2467 6475 2467 Size: 4008 2473 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4288 4670 5305 275 Size: 53 83 186 936 2755 382 635 1451 Fragments arranged by size: 2755 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2682 4914 5312 5586 5733 6144 2682 Size: 2232 398 274 147 411 3019 Fragments arranged by size: 3019 2232 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3723 3753 1178 Size: 30 165 30 2320 30 3906 Fragments arranged by size: 3906 2320 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3583 1186 Size: 2397 4084 BseYI C'CCAG_C Cuts at: 2748 3162 5684 6180 2748 Size: 414 2522 496 3049 Fragments arranged by size: 3049 2522 496 414 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2237 3053 3056 3483 1163 Size: 573 501 816 3 427 4161 Fragments arranged by size: 4161 816 573 501 427 3 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3931 4692 5720 1540 Size: 275 186 1930 761 1028 2301 Fragments arranged by size: 2301 1930 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4542 4627 5698 4542 Size: 85 1071 5325 Fragments arranged by size: 5325 1071 85 BsmI GAATG_Cn' Cuts at: 3992 4085 3992 Size: 93 6388 BsmBI CGTCTCn'nnnn_ Cuts at: 3311 3748 3311 Size: 437 6044 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2210 2256 3800 4542 4627 5698 1391 Size: 399 420 46 1544 742 85 1071 2174 Fragments arranged by size: 2174 1544 1071 742 420 399 85 46 BspEI T'CCGG_A Cuts at: 2682 6144 2682 Size: 3462 3019 BspHI T'CATG_A Cuts at: 4370 6100 4370 Size: 1730 4751 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2673 3078 3836 844 Size: 1829 405 758 3489 Fragments arranged by size: 3489 1829 758 405 BsrBI CCG'CTC Cuts at: 1394 1823 4368 1394 Size: 429 2545 3507 Fragments arranged by size: 3507 2545 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4976 5150 65 Size: 1183 3728 174 1396 Fragments arranged by size: 3728 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5125 5312 1700 Size: 110 3315 187 2869 Fragments arranged by size: 3315 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2385 96 Size: 2289 4192 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6481 BssSI C'ACGA_G Cuts at: 4541 5553 4541 Size: 1012 5469 BstAPI GCAn_nnn'nTGC Cuts at: 794 3529 794 Size: 2735 3746 BstBI TT'CG_AA Cuts at: 3287 5285 3287 Size: 1998 4483 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6481 BstYI r'GATC_y Cuts at: 1972 2016 2091 2346 2926 3736 3811 4178 4563 Size: 44 75 255 580 810 75 367 385 Cuts at: 4563 4580 6032 6118 6130 6476 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 810 580 385 367 346 255 86 75 75 44 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2211 513 Size: 552 939 207 4783 Fragments arranged by size: 4783 939 552 207 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5742 Fragments arranged by size: 5742 683 56 BtsI GCAGTG_nn' Cuts at: 2926 2991 3997 4791 4811 2926 Size: 65 1006 794 20 4596 Fragments arranged by size: 4596 1006 794 65 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6481 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6446 DraI TTT'AAA Cuts at: 3785 3785 Size: 6481 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5488 809 Size: 507 4172 1802 Fragments arranged by size: 4172 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2358 3928 4817 Size: 54 1425 50 275 18 528 1570 889 Cuts at: 4817 6446 8 Size: 1629 43 Fragments arranged by size: 1629 1570 1425 889 528 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3928 1537 Size: 275 2116 4090 Fragments arranged by size: 4090 2116 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2766 3372 4411 1353 Size: 1413 606 1039 3423 Fragments arranged by size: 3423 1413 1039 606 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5068 5442 5588 387 Size: 1064 138 3479 374 146 1280 Fragments arranged by size: 3479 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2108 2642 2648 3038 3221 3353 3396 Size: 393 476 534 6 390 183 132 43 Cuts at: 3396 3416 3492 3650 3725 4544 5141 5928 6196 Size: 20 76 158 75 819 597 787 268 Cuts at: 6196 1239 Size: 1524 Fragments arranged by size: 1524 819 787 597 534 476 393 390 268 183 158 132 76 75 43 20 6 EcoO109I rG'GnC_Cy Cuts at: 3648 3648 Size: 6481 EcoRI G'AATT_C Cuts at: 3790 4168 3790 Size: 378 6103 FspI TGC'GCA Cuts at: 4987 4987 Size: 6481 HaeII r_GCGC'y Cuts at: 1678 5628 1678 Size: 3950 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2059 2091 2574 2606 2664 Size: 32 135 32 487 32 483 32 58 Cuts at: 2664 2696 3209 3241 3559 3591 3640 3672 3721 Size: 32 513 32 318 32 49 32 49 Cuts at: 3721 3753 5122 5154 5196 5228 1373 Size: 32 1369 32 42 32 2626 Fragments arranged by size: 2626 1369 513 487 483 318 135 58 49 49 42 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2869 3825 4071 669 Size: 988 281 931 956 246 3079 Fragments arranged by size: 3079 988 956 931 281 246 HindIII A'AGCT_T Cuts at: 748 2741 748 Size: 1993 4488 HpaI GTT'AAC Cuts at: 4071 4071 Size: 6481 KpnI G_GTAC'C Cuts at: 2640 3806 2640 Size: 1166 5315 MfeI C'AATT_G Cuts at: 2130 4080 2130 Size: 1950 4531 MluI A'CGCG_T Cuts at: 6357 6357 Size: 6481 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5595 5779 1305 Size: 4290 184 2007 Fragments arranged by size: 4290 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4659 Fragments arranged by size: 4659 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2381 4439 4798 4957 518 Size: 648 1215 2058 359 159 2042 Fragments arranged by size: 2058 2042 1215 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2510 2981 3037 3897 4574 5722 5967 1606 Size: 904 471 56 860 677 1148 245 2120 Fragments arranged by size: 2120 1148 904 860 677 471 245 56 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4990 Fragments arranged by size: 4990 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6481 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6481 NotI GC'GGCC_GC Cuts at: 3928 3928 Size: 6481 NspI r_CATG'y Cuts at: 1132 1229 3771 3839 5384 1132 Size: 97 2542 68 1545 2229 Fragments arranged by size: 2542 2229 1545 97 68 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6481 PciI A'CATG_T Cuts at: 5380 5380 Size: 6481 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6481 PfoI T'CCnGG_A Cuts at: 2342 3054 2342 Size: 712 5769 PmeI GTTT'AAAC Cuts at: 3785 3785 Size: 6481 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4550 4582 6063 6095 4550 Size: 32 1481 32 4936 Fragments arranged by size: 4936 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3648 3648 Size: 6481 PshAI GACnn'nnGTC Cuts at: 1613 2903 1613 Size: 1290 5191 PsiI TTA'TAA Cuts at: 4051 4051 Size: 6481 PstI C_TGCA'G Cuts at: 830 2986 3390 3833 830 Size: 2156 404 443 3478 Fragments arranged by size: 3478 2156 443 404 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6481 PvuII CAG'CTG Cuts at: 2510 2981 3037 2510 Size: 471 56 5954 Fragments arranged by size: 5954 471 56 SalI G'TCGA_C Cuts at: 1936 3823 1936 Size: 1887 4594 SapI GCTCTTCn'nnn_ Cuts at: 2766 2766 Size: 6481 SbfI CC_TGCA'GG Cuts at: 3833 3833 Size: 6481 ScaI AGT'ACT Cuts at: 3344 4729 6441 3344 Size: 1385 1712 3384 Fragments arranged by size: 3384 1712 1385 SfcI C'TryA_G Cuts at: 826 1045 2982 3386 3829 4964 5645 5836 826 Size: 219 1937 404 443 1135 681 191 1471 Fragments arranged by size: 1937 1471 1135 681 443 404 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6481 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6481 SmaI CCC'GGG Cuts at: 1872 3808 1872 Size: 1936 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2753 4585 5486 5748 820 Size: 197 926 810 1832 901 262 1553 Fragments arranged by size: 1832 1553 926 901 810 262 197 SnaBI TAC'GTA Cuts at: 493 4272 493 Size: 3779 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6481 SphI G_CATG'C Cuts at: 1132 3839 1132 Size: 2707 3774 SspI AAT'ATT Cuts at: 5 52 4405 5 Size: 47 4353 2081 Fragments arranged by size: 4353 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6481 StyI C'CwwG_G Cuts at: 513 1065 2004 2958 3405 3920 513 Size: 552 939 954 447 515 3074 Fragments arranged by size: 3074 954 939 552 515 447 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4508 4525 4678 4863 5308 325 Size: 1255 2928 17 153 185 445 1498 Fragments arranged by size: 2928 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2385 2430 3342 Size: 274 80 33 51 1443 408 45 912 Cuts at: 3342 4727 6439 96 Size: 1385 1712 138 Fragments arranged by size: 1712 1443 1385 912 408 274 138 80 51 45 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2233 2265 2442 2474 2918 2950 6441 6473 2233 Size: 32 177 32 444 32 3491 32 2241 Fragments arranged by size: 3491 2241 444 177 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3620 1316 Size: 2304 4177 XbaI T'CTAG_A Cuts at: 2881 3256 3817 2881 Size: 375 561 5545 Fragments arranged by size: 5545 561 375 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2538 2538 Size: 6481 XhoI C'TCGA_G Cuts at: 1943 2753 1943 Size: 810 5671 XmaI C'CCGG_G Cuts at: 1870 3806 1870 Size: 1936 4545 XmnI GAAnn'nnTTC Cuts at: 2690 3283 3381 4610 2690 Size: 593 98 1229 4561 Fragments arranged by size: 4561 1229 593 98 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4289 276 Size: 53 83 186 936 2755 2468 Fragments arranged by size: 2755 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI