(Linear) (Six-base) MAPSORT of: KIEE4530.seq Check: 4038 from: 1 to: 1776 KIEE4530.orf With 182 enzymes: SgfI * March 22, 2012 18:17 .. Acc65I G'GTAC_C Cuts at: 0 631 1776 Size: 631 1145 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 103 637 643 1216 1348 1391 1487 1645 Size: 103 534 6 573 132 43 96 158 Cuts at: 1645 1776 Size: 131 Fragments arranged by size: 573 534 158 132 131 103 96 43 6 AflIII A'CryG_T Cuts at: 0 1476 1776 Size: 1476 300 AleI CACnn'nnGTG Cuts at: 0 376 1776 Size: 376 1400 AlwNI CAG_nnn'CTG Cuts at: 0 671 1056 1626 1776 Size: 671 385 570 150 Fragments arranged by size: 671 570 385 150 ApoI r'AATT_y Cuts at: 0 682 781 1274 1776 Size: 682 99 493 502 Fragments arranged by size: 682 502 493 99 AvaI C'yCGr_G Cuts at: 0 748 1776 Size: 748 1028 BanI G'GyrC_C Cuts at: 0 631 1776 Size: 631 1145 BanII G_rGCy'C Cuts at: 0 205 1776 Size: 205 1571 BbsI GAAGACnn'nnnn_ Cuts at: 0 1092 1776 Size: 1092 684 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 357 391 1776 Size: 357 34 1385 Fragments arranged by size: 1385 357 34 BciVI GTATCCnnnnn_n' Cuts at: 0 685 1355 1776 Size: 685 670 421 Fragments arranged by size: 685 670 421 Bme1580I G_kGCm'C Cuts at: 0 251 1776 Size: 251 1525 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1033 1411 1720 1776 Size: 1033 378 309 56 Fragments arranged by size: 1033 378 309 56 Bpu10I CC'TnA_GC Cuts at: 0 364 1221 1776 Size: 364 857 555 Fragments arranged by size: 857 555 364 BsaBI GATnn'nnATC Cuts at: 0 462 1776 Size: 462 1314 BsaWI w'CCGG_w Cuts at: 0 677 1776 Size: 677 1099 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1718 1748 1776 Size: 1718 30 28 Fragments arranged by size: 1718 30 28 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1578 1776 Size: 1578 198 BseYI C'CCAG_C Cuts at: 0 743 1157 1776 Size: 743 414 619 Fragments arranged by size: 743 619 414 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 232 1048 1051 1478 1776 Size: 232 816 3 427 298 Fragments arranged by size: 816 427 298 232 3 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1306 1743 1776 Size: 1306 437 33 Fragments arranged by size: 1306 437 33 Bsp1286I G_dGCh'C Cuts at: 0 205 251 1776 Size: 205 46 1525 Fragments arranged by size: 1525 205 46 BspEI T'CCGG_A Cuts at: 0 677 1776 Size: 677 1099 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 668 1073 1776 Size: 668 405 703 Fragments arranged by size: 703 668 405 BsrGI T'GTAC_A Cuts at: 0 380 1776 Size: 380 1396 BstAPI GCAn_nnn'nTGC Cuts at: 0 1524 1776 Size: 1524 252 BstBI TT'CG_AA Cuts at: 0 1282 1776 Size: 1282 494 BstYI r'GATC_y Cuts at: 0 11 86 341 921 1731 1776 Size: 11 75 255 580 810 45 Fragments arranged by size: 810 580 255 75 45 11 BtgI C'CryG_G Cuts at: 0 206 1776 Size: 206 1570 BtsI GCAGTG_nn' Cuts at: 0 921 986 1776 Size: 921 65 790 Fragments arranged by size: 921 790 65 EaeI y'GGCC_r Cuts at: 0 353 1776 Size: 353 1423 EarI CTCTTCn'nnn_ Cuts at: 0 761 1367 1776 Size: 761 606 409 Fragments arranged by size: 761 606 409 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 103 637 643 1033 1216 1348 1391 1411 Size: 103 534 6 390 183 132 43 20 Cuts at: 1411 1487 1645 1720 1776 Size: 76 158 75 56 Fragments arranged by size: 534 390 183 158 132 103 76 75 56 43 20 6 EcoO109I rG'GnC_Cy Cuts at: 0 1643 1776 Size: 1643 133 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 54 86 569 601 659 691 1204 1236 Size: 54 32 483 32 58 32 513 32 Cuts at: 1236 1554 1586 1635 1667 1716 1748 1776 Size: 318 32 49 32 49 32 28 Fragments arranged by size: 513 483 318 58 54 49 49 32 32 32 32 32 32 32 28 HincII GTy'rAC Cuts at: 0 864 1776 Size: 864 912 HindIII A'AGCT_T Cuts at: 0 736 1776 Size: 736 1040 KpnI G_GTAC'C Cuts at: 0 635 1776 Size: 635 1141 MfeI C'AATT_G Cuts at: 0 125 1776 Size: 125 1651 MslI CAynn'nnrTG Cuts at: 0 376 1776 Size: 376 1400 MspA1I CmG'CkG Cuts at: 0 505 976 1032 1776 Size: 505 471 56 744 Fragments arranged by size: 744 505 471 56 NspI r_CATG'y Cuts at: 0 1766 1776 Size: 1766 10 PfoI T'CCnGG_A Cuts at: 0 337 1049 1776 Size: 337 712 727 Fragments arranged by size: 727 712 337 PpuMI rG'GwC_Cy Cuts at: 0 1643 1776 Size: 1643 133 PshAI GACnn'nnGTC Cuts at: 0 898 1776 Size: 898 878 PstI C_TGCA'G Cuts at: 0 981 1385 1776 Size: 981 404 391 Fragments arranged by size: 981 404 391 PvuII CAG'CTG Cuts at: 0 505 976 1032 1776 Size: 505 471 56 744 Fragments arranged by size: 744 505 471 56 SapI GCTCTTCn'nnn_ Cuts at: 0 761 1776 Size: 761 1015 ScaI AGT'ACT Cuts at: 0 1339 1776 Size: 1339 437 SfcI C'TryA_G Cuts at: 0 977 1381 1776 Size: 977 404 395 Fragments arranged by size: 977 404 395 SmlI C'TyrA_G Cuts at: 0 748 1776 Size: 748 1028 StyI C'CwwG_G Cuts at: 0 953 1400 1776 Size: 953 447 376 Fragments arranged by size: 953 447 376 TatI w'GTAC_w Cuts at: 0 380 425 1337 1776 Size: 380 45 912 439 Fragments arranged by size: 912 439 380 45 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 228 260 437 469 913 945 1776 Size: 228 32 177 32 444 32 831 Fragments arranged by size: 831 444 228 177 32 32 32 Tth111I GACn'n_nGTC Cuts at: 0 1615 1776 Size: 1615 161 XbaI T'CTAG_A Cuts at: 0 876 1251 1776 Size: 876 375 525 Fragments arranged by size: 876 525 375 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 533 1776 Size: 533 1243 XhoI C'TCGA_G Cuts at: 0 748 1776 Size: 748 1028 XmnI GAAnn'nnTTC Cuts at: 0 685 1278 1376 1776 Size: 685 593 98 400 Fragments arranged by size: 685 593 400 98 Enzymes that do cut: Acc65I AcuI AflIII AleI AlwNI ApoI AvaI BanI BanII BbsI BcgI BciVI Bme1580I BpmI Bpu10I BsaBI BsaWI BsaXI BseRI BseYI BsgI BsmBI Bsp1286I BspEI BspMI BsrGI BstAPI BstBI BstYI BtgI BtsI EaeI EarI Eco57MI EcoO109I Hin4I HincII HindIII KpnI MfeI MslI MspA1I NspI PfoI PpuMI PshAI PstI PvuII SapI ScaI SfcI SmlI StyI TatI TstI Tth111I XbaI XcmI XhoI XmnI Enzymes that do not cut: AarI AatII AccI AclI AfeI AflII AgeI AhdI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BclI BfrBI BglI BglII BlpI BmgBI BmrI BmtI BplI BpuEI BsaI BsaAI BsaHI BsiEI BsiHKAI BsiWI BsmI BspHI BsrBI BsrDI BsrFI BssHII BssSI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HaeII HpaI KasI MluI MmeI MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PflMI PmeI PmlI PpiI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII XmaI ZraI