################################## # NO CUT ENZYMES ( 144 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaII AvrII BalI BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BlnI BlpI BpmI Bpu1102I BsaBI BsaI BsaWI BseAI BsgI BsiHKAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrGI BssSI Bst1107I BstAPI BstEII BstPI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT22I FbaI FseI HindIII HpaI KasI KpnI Ksp632I KspI MaeI MamI MfeI MluI MluNI MroI MscI MunI NarI NciI NcoI NdeI NheI NotI NruI NsiI PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI Tth111I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AflIII 231 ACGTGT A^C[GA][CT]GT AlwNI 94 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 393 GGGCCC GGGCC^C Asp700I 880 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 341 CCCGAG C^[CT]CG[GA]G AviII 711 TGCGCA TGC^GCA BanII 393 GGGCCC G[GA]GC[CT]^C BglI 24 GCCGCGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 555 AGATCT A^GATCT BsaAI 699 CACGTA [CT]AC^GT[GA] BseRI 256 GAGGAG GAGGAG(10/8) BsiEI 144 CGACCG CG[GA][CT]^CG BsoBI 341 CCCGAG C^[CT]CG[GA]G BsrBI 55 GAGCGG CCGCTC(-3/-3) BssHII 187 GCGCGC G^CGCGC BstBI 845 TTCGAA TT^CGAA EaeI 8 TGGCCG [CT]^GGCC[GA] EcoRI 208 GAATTC G^AATTC EcoT14I 308 CCAAGG C^C[AT][AT]GG FspI 711 TGCGCA TGC^GCA HaeII 570 GGCGCT [GA]GCGC^[CT] HincII 472 GTTGAC GT[CT]^[GA]AC HindII 472 GTTGAC GT[CT]^[GA]AC MaeIII 737 GTGAC ^GT[ACGT]AC MseI 920 TTAA T^TAA MslI 699 CACGTACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NspI 179 GCATGC [GA]CATG^[CT] NspV 845 TTCGAA TT^CGAA PflMI 397 CCAGTTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PstI 91 CTGCAG CTGCA^G PvuII 631 CAGCTG CAG^CTG SfuI 845 TTCGAA TT^CGAA SphI 179 GCATGC GCATG^C StyI 308 CCAAGG C^C[AT][AT]GG Tru9I 920 TTAA T^TAA Tsp45I 737 GTGAC ^GT[GC]AC Van91I 397 CCAGTTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 880 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AcsI 208 GAATTC [GA]^AATT[CT] 797 AAATTC AcyI 61 GACGCC G[GA]^CG[CT]C 130 GGCGTC ApoI 208 GAATTC [GA]^AATT[CT] 797 AAATTC BamHI 280 GGATCC G^GATCC 337 GGATCC BanI 154 GGCACC G^G[CT][GA]CC 160 GGCACC BbsI 880 GAAGAC GAAGAC(2/6) 303 GTCTTC BmyI 153 GGGCAC G[AGT]GC[ACT]^C 393 GGGCCC BpuAI 880 GAAGAC GAAGAC(2/6) 303 GTCTTC BsaHI 61 GACGCC G[GA]^CG[CT]C 130 GGCGTC BsmAI 344 GAGAC GTCTC(1/5) 650 GAGAC BsmFI 59 GGGAC GGGAC(10/14) 386 GGGAC Bsp1286I 153 GGGCAC G[AGT]GC[ACT]^C 393 GGGCCC DdeI 199 CTGAG C^T[ACGT]AG 835 CTCAG FauI 43 CCCGC CCCGC(4/6) 57 GCGGG Hin1I 61 GACGCC G[GA]^CG[CT]C 130 GGCGTC HphI 609 GGTGA GGTGA(8/7) 736 GGTGA MaeII 231 ACGT A^CGT 700 ACGT MspA1I 123 CCGCTG C[AC]G^C[GT]G 631 CAGCTG NaeI 10 GCCGGC GCC^GGC 29 GCCGGC NgoMIV 10 GCCGGC G^CCGGC 29 GCCGGC SfcI 91 CTGCAG C^T[GA][CT]AG 192 CTACAG TaqI 785 TCGA T^CGA 846 TCGA TthHB8I 785 TCGA T^CGA 846 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 53 enzymes) # ######################################### AccII AciI AfaI AluI AlwI BbvI BsaJI BsiYI BslI BsrFI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DpnII EcoRII Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII ItaI MboI MboII MflI MnlI MspI MvaI MvnI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI TfiI TseI Tsp509I TspRI XhoII