################################## # NO CUT ENZYMES ( 136 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AcyI AflII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI ApoI AscI Asp700I Asp718I AspEI AspI AvaI AviII BalI BamHI BanI BbeI BbrPI BciVI BclI BfrI BglI BglII BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BsoBI BspDI BspEI BspHI BsrBI BsrFI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I CfoI Cfr10I ClaI CpoI DraI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HpaI KasI KpnI Ksp632I KspI MamI MfeI MluI MluNI MroI MscI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI Psp1406I PstI PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI Sse8387I StuI SwaI TaqI Tth111I TthHB8I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AflIII 1441 ACATGT A^C[GA][CT]GT AocI 476 CCTTAGG CC^T[ACGT]AGG AseI 1183 ATTAAT AT^TAAT AsnI 1183 ATTAAT AT^TAAT AspHI 435 GAGCTC G[AT]GC[AT]^C AvrII 330 CCTAGG C^CTAGG BbvCI 910 CCTCAGC CCTCAGC(-5/-2) BcgI 1073 GCAGTGGGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 1230 CCGGG CC^[GC]GG BlnI 330 CCTAGG C^CTAGG BpmI 680 CTCCAG CTGGAG(16/14) BsiHKAI 435 GAGCTC G[AT]GC[AT]^C Bsp1407I 66 TGTACA T^GTACA BspLU11I 1441 ACATGT A^CATGT BsrGI 66 TGTACA T^GTACA BstAPI 139 GCATATACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 476 CCTTAGG CC^T[ACGT]AGG DraIII 1156 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 29 GACCAAAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 1068 CCGTGG C^C[GA][CT]GG Eco81I 476 CCTTAGG CC^T[ACGT]AGG EcoNI 525 CCTCAATAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT22I 137 ATGCAT ATGCA^T HapII 1230 CCGG C^CGG HincII 1381 GTCAAC GT[CT]^[GA]AC HindII 1381 GTCAAC GT[CT]^[GA]AC HindIII 1171 AAGCTT A^AGCTT HpaII 1230 CCGG C^CGG MspI 1230 CCGG C^CGG NciI 1230 CCGGG CC^[GC]GG NheI 727 GCTAGC G^CTAGC NsiI 137 ATGCAT ATGCA^T NspI 1441 ACATGT [GA]CATG^[CT] PleI 114 GAGTC GAGTC(4/5) PpuMI 843 GGGTCCC [GA]G^G[AT]CC[CT] PshBI 1183 ATTAAT AT^TAAT PvuII 271 CAGCTG CAG^CTG SacI 435 GAGCTC GAGCT^C SmlI 111 CTTGAG C^T[CT][GA]AG SpeI 649 ACTAGT A^CTAGT SspBI 66 TGTACA T^GTACA SspI 1288 AATATT AAT^ATT XcmI 8 CCAGCAGCCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AccI 225 GTCTAC GT^[AC][GT]AC 426 GTCTAC AfaI 52 GTAC GT^AC 67 GTAC AlwI 61 GGATC GGATC(4/5) 1135 GGATC BbsI 759 GAAGAC GAAGAC(2/6) 788 GTCTTC BpuAI 759 GAAGAC GAAGAC(2/6) 788 GTCTTC BseRI 695 CTCCTC GAGGAG(10/8) 908 CTCCTC BsgI 1296 GTGCAG GTGCAG(16/14) 1445 GTGCAG BspMI 1447 GCAGGT ACCTGC(4/8) 1614 GCAGGT BstXI 706 CCAGCATCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1042 CCAACCCGCTGG BstYI 61 GGATCT [GA]^GATC[CT] 1135 GGATCT DpnII 62 GATC ^GATC 1136 GATC DraII 843 GGGTCCC [GA]G^G[ACGT]CC[CT] 1356 AGGCCCC EaeI 5 CGGCCA [CT]^GGCC[GA] 1227 TGGCCG EcoO109I 843 GGGTCCC [GA]G^G[ACGT]CC[CT] 1356 AGGCCCC EcoT14I 330 CCTAGG C^C[AT][AT]GG 558 CCAAGG FauI 1046 CCCGC CCCGC(4/6) 638 GCGGG MaeII 1511 ACGT A^CGT 1719 ACGT MboI 62 GATC ^GATC 1136 GATC MflI 61 GGATCT [GA]^GATC[CT] 1135 GGATCT MslI 240 CACAGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1396 CACTTCTGTG MspA1I 271 CAGCTG C[AC]G^C[GT]G 1047 CCGCTG NdeII 62 GATC ^GATC 1136 GATC PflMI 343 CCATTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 558 CCAAGGGATGG RsaI 52 GTAC GT^AC 67 GTAC Sau3AI 62 GATC ^GATC 1136 GATC StyI 330 CCTAGG C^C[AT][AT]GG 558 CCAAGG TfiI 157 GATTC G^A[AT]TC 1215 GAATC Tsp45I 236 GTGAC ^GT[GC]AC 1567 GTGAC Van91I 343 CCATTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 558 CCAAGGGATGG XhoII 61 GGATCT [GA]^GATC[CT] 1135 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 49 enzymes) # ######################################### AciI AluI AvaII BanII BbvI BfaI BlpI BmyI Bpu1102I BsaI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI Cac8I CelII Cfr13I DdeI EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI MaeI MaeIII MboII MnlI MseI MvaI MwoI NlaIII NlaIV Sau96I ScrFI SfaNI SfcI Tru9I TseI Tsp509I TspRI