Restriction map for
pFN21AE5602
(Circular) (Six-base) MAPSORT of: pFN21AE5602.seq Check: 2004 from: 1 to: 6442
pFN21AE5602 6442 bp
With 182 enzymes: SgfI *
December 8, 2014 13:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3443 3610 3443
Size: 167 6275
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4252 278
Size: 53 83 186 936 2716 2468
Fragments arranged by size:
2716 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2231 2432 3785 1937
Size: 294 201 1353 4594
Fragments arranged by size:
4594 1353 294 201
Acc65I G'GTAC_C
Cuts at: 3763 3763
Size: 6442
AclI AA'CG_TT
Cuts at: 1563 4569 4942 1563
Size: 3006 373 3063
Fragments arranged by size:
3063 3006 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2600 2783 3135 4505 5889 6157 1632
Size: 968 183 352 1370 1384 268 1917
Fragments arranged by size:
1917 1384 1370 968 352 268 183
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6245
AflIII A'CryG_T
Cuts at: 1203 3446 5341 6318 1203
Size: 2243 1895 977 1327
Fragments arranged by size:
2243 1895 1327 977
AgeI A'CCGG_T
Cuts at: 5273 5273
Size: 6442
AhdI GACnn_n'nnGTC
Cuts at: 5171 5171
Size: 6442
AleI CACnn'nnGTG
Cuts at: 1166 3405 1166
Size: 2239 4203
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6442
AlwNI CAG_nnn'CTG
Cuts at: 4185 5757 4185
Size: 1572 4870
ApaLI G'TGCA_C
Cuts at: 4499 5655 4499
Size: 1156 5286
ApoI r'AATT_y
Cuts at: 1493 3751 3977 4129 6238 1493
Size: 2258 226 152 2109 1697
Fragments arranged by size:
2258 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3189 4996 160
Size: 872 2157 1807 1606
Fragments arranged by size:
2157 1807 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6442
AvaI C'yCGr_G
Cuts at: 1870 1943 3767 1870
Size: 73 1824 4545
Fragments arranged by size:
4545 1824 73
AvrII C'CTAG_G
Cuts at: 2335 2335
Size: 6442
BamHI G'GATC_C
Cuts at: 3772 4139 3772
Size: 367 6075
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3763 5218 618
Size: 325 217 627 1976 1455 1842
Fragments arranged by size:
1976 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2295 2335 2444 3761 1391
Size: 904 40 109 1317 4072
Fragments arranged by size:
4072 1317 904 109 40
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2771 2786 928
Size: 971 872 15 4584
Fragments arranged by size:
4584 971 872 15
BbvCI CC'TCA_GC
Cuts at: 2916 2916
Size: 6442
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3067 3101 4633 4667 1903
Size: 34 1130 34 1532 34 3678
Fragments arranged by size:
3678 1532 1130 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4334 5544 4334
Size: 1210 5232
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6442
BfrBI ATG'CAT
Cuts at: 2144 2144
Size: 6442
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 5053 136
Size: 107 122 71 631 605 337 3044 1525
Fragments arranged by size:
3044 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 6437 6437
Size: 6442
BlpI GC'TnA_GC
Cuts at: 2279 3042 3615 3859 2279
Size: 763 573 244 4862
Fragments arranged by size:
4862 763 573 244
Bme1580I G_kGCm'C
Cuts at: 1790 4503 5659 1790
Size: 2713 1156 2573
Fragments arranged by size:
2713 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6442
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2169 2376 3158
Size: 416 53 83 399 24 752 207 782
Cuts at: 3158 4173 5131 442
Size: 1015 958 1753
Fragments arranged by size:
1753 1015 958 782 752 416 399 207
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2736 1056
Size: 1680 4762
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2670 5102 1239
Size: 1431 2432 2579
Fragments arranged by size:
2579 2432 1431
Bpu10I CC'TnA_GC
Cuts at: 2916 2958 2916
Size: 42 6400
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2137 4567 5432 5730 2137
Size: 2430 865 298 2849
Fragments arranged by size:
2849 2430 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2026 2519 2916 5105 882
Size: 617 527 493 397 2189 2219
Fragments arranged by size:
2219 2189 617 527 493 397
BsaAI yAC'GTr
Cuts at: 493 1204 4233 493
Size: 711 3029 2702
Fragments arranged by size:
3029 2702 711
BsaBI GATnn'nnATC
Cuts at: 6436 6436
Size: 6442
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4249 4631 5266 275
Size: 53 83 186 936 2716 382 635 1451
Fragments arranged by size:
2716 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4875 5273 5547 5694 6105 4875
Size: 398 274 147 411 5212
Fragments arranged by size:
5212 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2174 2204 2683 2713 1178
Size: 30 165 30 771 30 479 30 4907
Fragments arranged by size:
4907 771 479 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2691 2904 1186
Size: 1505 213 4724
Fragments arranged by size:
4724 1505 213
BseYI C'CCAG_C
Cuts at: 3015 3054 5645 6141 3015
Size: 39 2591 496 3316
Fragments arranged by size:
3316 2591 496 39
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3322 3471 1163
Size: 573 1586 149 4134
Fragments arranged by size:
4134 1586 573 149
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3892 4653 5681 1540
Size: 275 186 1891 761 1028 2301
Fragments arranged by size:
2301 1891 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2444 4503 4588 5659 2444
Size: 2059 85 1071 3227
Fragments arranged by size:
3227 2059 1071 85
BsmI GAATG_Cn'
Cuts at: 3953 4046 3953
Size: 93 6349
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2295 2335 2444 3761 4503 4588 5659
Size: 399 505 40 109 1317 742 85 1071
Cuts at: 5659 1391
Size: 2174
Fragments arranged by size:
2174 1317 1071 742 505 399 109 85
40
BspEI T'CCGG_A
Cuts at: 6105 6105
Size: 6442
BspHI T'CATG_A
Cuts at: 4331 6061 4331
Size: 1730 4712
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3443 3610 3797 844
Size: 2599 167 187 3489
Fragments arranged by size:
3489 2599 187 167
BsrBI CCG'CTC
Cuts at: 1394 1823 4329 1394
Size: 429 2506 3507
Fragments arranged by size:
3507 2506 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2319 2614 2694 2778 2987 4937 5111
Size: 1183 1071 295 80 84 209 1950 174
Cuts at: 5111 65
Size: 1396
Fragments arranged by size:
1950 1396 1183 1071 295 209 174 84
80
BsrFI r'CCGG_y
Cuts at: 1700 1810 5086 5273 1700
Size: 110 3276 187 2869
Fragments arranged by size:
3276 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2071 96
Size: 1975 4467
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6442
BssSI C'ACGA_G
Cuts at: 4502 5514 4502
Size: 1012 5430
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2150 794
Size: 1356 5086
BstBI TT'CG_AA
Cuts at: 5246 5246
Size: 6442
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6442
BstXI CCAn_nnnn'nTGG
Cuts at: 2718 3054 2718
Size: 336 6106
BstYI r'GATC_y
Cuts at: 1972 2066 3140 3772 4139 4524 4541 5993 6079
Size: 94 1074 632 367 385 17 1452 86
Cuts at: 6079 6091 6437 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1074 632 385 367 346 94
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2482 2482
Size: 6442
BtgI C'CryG_G
Cuts at: 513 1065 3073 513
Size: 552 2008 3882
Fragments arranged by size:
3882 2008 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5703
Fragments arranged by size:
5703 683 56
BtsI GCAGTG_nn'
Cuts at: 3085 3400 3958 4752 4772 3085
Size: 315 558 794 20 4755
Fragments arranged by size:
4755 794 558 315 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6442
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3376 3411 529
Size: 35 2812 35 3560
Fragments arranged by size:
3560 2812 35 35
DraI TTT'AAA
Cuts at: 3746 3746
Size: 6442
DraIII CAC_nnn'GTG
Cuts at: 3166 3166
Size: 6442
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2040 5449 809
Size: 507 724 3409 1802
Fragments arranged by size:
3409 1802 724 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2010 3232 3889
Size: 54 1425 50 275 18 180 1222 657
Cuts at: 3889 4778 6407 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1222 889 657 275 180 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3889 1537
Size: 275 2077 4090
Fragments arranged by size:
4090 2077 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4372 1353
Size: 3019 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2787 5029 5403 5549 387
Size: 1064 138 1198 2242 374 146 1280
Fragments arranged by size:
2242 1280 1198 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2442 2442
Size: 6442
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2600 2670 2783 3135 4505 5102 5889
Size: 393 968 70 113 352 1370 597 787
Cuts at: 5889 6157 1239
Size: 268 1524
Fragments arranged by size:
1524 1370 968 787 597 393 352 268
113 70
EcoNI CCTnn'n_nnAGG
Cuts at: 2534 2534
Size: 6442
EcoO109I rG'GnC_Cy
Cuts at: 2849 3362 2849
Size: 513 5929
EcoRI G'AATT_C
Cuts at: 3751 4129 3751
Size: 378 6064
FspI TGC'GCA
Cuts at: 4948 4948
Size: 6442
HaeII r_GCGC'y
Cuts at: 1678 5589 1678
Size: 3911 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2492 2524 3659 3691 5083
Size: 32 135 32 920 32 1135 32 1392
Cuts at: 5083 5115 5157 5189 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1392 1135 920 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3388 3786 4032 669
Size: 988 281 1450 398 246 3079
Fragments arranged by size:
3079 1450 988 398 281 246
HindIII A'AGCT_T
Cuts at: 748 3176 748
Size: 2428 4014
HpaI GTT'AAC
Cuts at: 4032 4032
Size: 6442
KpnI G_GTAC'C
Cuts at: 3767 3767
Size: 6442
MfeI C'AATT_G
Cuts at: 4041 4041
Size: 6442
MluI A'CGCG_T
Cuts at: 6318 6318
Size: 6442
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3150 5556 5740 1305
Size: 1845 2406 184 2007
Fragments arranged by size:
2406 2007 1845 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4620
Fragments arranged by size:
4620 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2249 3405 4400 4759 4918 518
Size: 648 1083 1156 995 359 159 2042
Fragments arranged by size:
2042 1156 1083 995 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2278 3054 3858 4535 5683 5928 1606
Size: 672 776 804 677 1148 245 2120
Fragments arranged by size:
2120 1148 804 776 677 672 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5890
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6442
NheI G'CTAG_C
Cuts at: 1052 2732 1052
Size: 1680 4762
NotI GC'GGCC_GC
Cuts at: 3889 3889
Size: 6442
NsiI A_TGCA'T
Cuts at: 2146 2146
Size: 6442
NspI r_CATG'y
Cuts at: 1132 1229 3450 3800 5345 1132
Size: 97 2221 350 1545 2229
Fragments arranged by size:
2229 2221 1545 350 97
PasI CC'CwG_GG
Cuts at: 1348 2905 1348
Size: 1557 4885
PciI A'CATG_T
Cuts at: 3446 5341 3446
Size: 1895 4547
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2354 2569 1108
Size: 1246 215 4981
Fragments arranged by size:
4981 1246 215
PmeI GTTT'AAAC
Cuts at: 3746 3746
Size: 6442
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4511 4543 6024 6056 4511
Size: 32 1481 32 4897
Fragments arranged by size:
4897 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2849 2849
Size: 6442
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6442
PsiI TTA'TAA
Cuts at: 4012 4012
Size: 6442
PstI C_TGCA'G
Cuts at: 830 3794 830
Size: 2964 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6442
PvuII CAG'CTG
Cuts at: 2278 2278
Size: 6442
SacI G_AGCT'C
Cuts at: 2444 2444
Size: 6442
SalI G'TCGA_C
Cuts at: 1936 3784 1936
Size: 1848 4594
SanDI GG'GwC_CC
Cuts at: 2849 2849
Size: 6442
SbfI CC_TGCA'GG
Cuts at: 3794 3794
Size: 6442
ScaI AGT'ACT
Cuts at: 4690 6402 4690
Size: 1712 4730
SfcI C'TryA_G
Cuts at: 826 1045 2182 2556 2922 3790 4925 5606 5797
Size: 219 1137 374 366 868 1135 681 191
Cuts at: 5797 826
Size: 1471
Fragments arranged by size:
1471 1137 1135 868 681 374 366 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6442
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6442
SmaI CCC'GGG
Cuts at: 1872 3769 1872
Size: 1897 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2116 4546 5447 5709 820
Size: 197 926 173 2430 901 262 1553
Fragments arranged by size:
2430 1553 926 901 262 197 173
SnaBI TAC'GTA
Cuts at: 493 4233 493
Size: 3740 2702
SpeI A'CTAG_T
Cuts at: 152 2654 152
Size: 2502 3940
SphI G_CATG'C
Cuts at: 1132 3800 1132
Size: 2668 3774
SspI AAT'ATT
Cuts at: 5 52 3295 4366 5
Size: 47 3243 1071 2081
Fragments arranged by size:
3243 2081 1071 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6442
StyI C'CwwG_G
Cuts at: 513 1065 2335 2563 3881 513
Size: 552 1270 228 1318 3074
Fragments arranged by size:
3074 1318 1270 552 228
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2204 3077 3171 3177 4469 4486 4639
Size: 1255 624 873 94 6 1292 17 153
Cuts at: 4639 4824 5269 325
Size: 185 445 1498
Fragments arranged by size:
1498 1292 1255 873 624 445 185 153
94 17 6
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2056 2071 4688
Size: 274 80 33 51 1443 79 15 2617
Cuts at: 4688 6400 96
Size: 1712 138
Fragments arranged by size:
2617 1712 1443 274 138 80 79 51
33 15
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2683 2715 3641 3673 6402 6434 2683
Size: 32 926 32 2729 32 2691
Fragments arranged by size:
2729 2691 926 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6442
XbaI T'CTAG_A
Cuts at: 3778 3778
Size: 6442
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2020 2020
Size: 6442
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6442
XmaI C'CCGG_G
Cuts at: 1870 3767 1870
Size: 1897 4545
XmnI GAAnn'nnTTC
Cuts at: 4571 4571
Size: 6442
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4250 276
Size: 53 83 186 936 2716 2468
Fragments arranged by size:
2716 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI BaeI BbeI BplI BsiWI
BsmBI BstZ17I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI
PsrI RsrII SacII SapI SexAI SfiI SfoI SrfI
SwaI