(Circular) (Six-base) MAPSORT of: pFN21AB6922.seq Check: 5458 from: 1 to: 5386 pFN21AB6922 5386 bp With 182 enzymes: SgfI * August 27, 2010 15:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3196 278 Size: 53 83 186 936 1660 2468 Fragments arranged by size: 2468 1660 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2729 1937 Size: 792 4594 Acc65I G'GTAC_C Cuts at: 2707 2707 Size: 5386 AclI AA'CG_TT Cuts at: 1563 3513 3886 1563 Size: 1950 373 3063 Fragments arranged by size: 3063 1950 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2320 3449 4833 5101 1632 Size: 688 1129 1384 268 1917 Fragments arranged by size: 1917 1384 1129 688 268 AfeI AGC'GCT Cuts at: 2654 2654 Size: 5386 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5189 AflIII A'CryG_T Cuts at: 1203 4285 5262 1203 Size: 3082 977 1327 Fragments arranged by size: 3082 1327 977 AgeI A'CCGG_T Cuts at: 4217 4217 Size: 5386 AhdI GACnn_n'nnGTC Cuts at: 4115 4115 Size: 5386 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5386 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2531 1184 Size: 1347 4039 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2087 2119 2087 Size: 32 5354 AlwNI CAG_nnn'CTG Cuts at: 2423 3129 4701 2423 Size: 706 1572 3108 Fragments arranged by size: 3108 1572 706 ApaI G_GGCC'C Cuts at: 2082 2082 Size: 5386 ApaLI G'TGCA_C Cuts at: 3443 4599 3443 Size: 1156 4230 ApoI r'AATT_y Cuts at: 1493 2695 2921 3073 5182 1493 Size: 1202 226 152 2109 1697 Fragments arranged by size: 2109 1697 1202 226 152 AseI AT'TA_AT Cuts at: 160 1032 3940 160 Size: 872 2908 1606 Fragments arranged by size: 2908 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5386 AvaI C'yCGr_G Cuts at: 1870 1943 2171 2432 2711 1870 Size: 73 228 261 279 4545 Fragments arranged by size: 4545 279 261 228 73 BamHI G'GATC_C Cuts at: 2716 3083 2716 Size: 367 5019 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2677 2707 4162 618 Size: 325 217 627 890 30 1455 1842 Fragments arranged by size: 1842 1455 890 627 325 217 30 BanII G_rGCy'C Cuts at: 1391 2082 2234 2433 2705 1391 Size: 691 152 199 272 4072 Fragments arranged by size: 4072 691 272 199 152 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4415 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3577 3611 1903 Size: 34 1640 34 3678 Fragments arranged by size: 3678 1640 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3278 4488 3278 Size: 1210 4176 BclI T'GATC_A Cuts at: 1548 1548 Size: 5386 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2021 2238 Size: 107 122 71 631 605 337 12 217 Cuts at: 2238 2540 3997 136 Size: 302 1457 1525 Fragments arranged by size: 1525 1457 631 605 337 302 217 122 107 71 12 BglII A'GATC_T Cuts at: 5381 5381 Size: 5386 BlpI GC'TnA_GC Cuts at: 2803 2803 Size: 5386 Bme1580I G_kGCm'C Cuts at: 1790 2082 2682 3447 4603 1790 Size: 292 600 765 1156 2573 Fragments arranged by size: 2573 1156 765 600 292 BmgBI CAC'GTC Cuts at: 1318 2212 1318 Size: 894 4492 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3117 4075 442 Size: 416 53 83 399 24 1700 958 1753 Fragments arranged by size: 1753 1700 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5386 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2246 2372 2519 4046 1239 Size: 1007 126 147 1527 2579 Fragments arranged by size: 2579 1527 1007 147 126 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2633 3511 4376 4674 2633 Size: 878 865 298 3345 Fragments arranged by size: 3345 878 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2168 2474 4049 882 Size: 617 669 306 1575 2219 Fragments arranged by size: 2219 1575 669 617 306 BsaAI yAC'GTr Cuts at: 493 1204 2546 3177 493 Size: 711 1342 631 2702 Fragments arranged by size: 2702 1342 711 631 BsaBI GATnn'nnATC Cuts at: 5380 5380 Size: 5386 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3193 3575 4210 275 Size: 53 83 186 936 1660 382 635 1451 Fragments arranged by size: 1660 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3819 4217 4491 4638 5049 3819 Size: 398 274 147 411 4156 Fragments arranged by size: 4156 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5161 Fragments arranged by size: 5161 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2414 2450 1186 Size: 1228 36 4122 Fragments arranged by size: 4122 1228 36 BseYI C'CCAG_C Cuts at: 4589 5085 4589 Size: 496 4890 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2626 1163 Size: 573 890 3923 Fragments arranged by size: 3923 890 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2016 2836 3597 4625 1540 Size: 275 186 15 820 761 1028 2301 Fragments arranged by size: 2301 1028 820 761 275 186 15 BsiHKAI G_wGCw'C Cuts at: 3447 3532 4603 3447 Size: 85 1071 4230 Fragments arranged by size: 4230 1071 85 BsmI GAATG_Cn' Cuts at: 2897 2990 2897 Size: 93 5293 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2082 2234 2433 2682 2705 3447 3532 Size: 399 292 152 199 249 23 742 85 Cuts at: 3532 4603 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 292 249 199 152 85 23 BspEI T'CCGG_A Cuts at: 5049 5049 Size: 5386 BspHI T'CATG_A Cuts at: 3275 5005 3275 Size: 1730 3656 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2565 2626 2741 844 Size: 1721 61 115 3489 Fragments arranged by size: 3489 1721 115 61 BsrBI CCG'CTC Cuts at: 1394 1823 2578 3273 1394 Size: 429 755 695 3507 Fragments arranged by size: 3507 755 695 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3881 4055 65 Size: 1183 2633 174 1396 Fragments arranged by size: 2633 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2015 2119 2155 4030 4217 1700 Size: 110 205 104 36 1875 187 2869 Fragments arranged by size: 2869 1875 205 187 110 104 36 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5386 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5386 BssSI C'ACGA_G Cuts at: 3446 4458 3446 Size: 1012 4374 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5386 BstBI TT'CG_AA Cuts at: 4190 4190 Size: 5386 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5386 BstYI r'GATC_y Cuts at: 1972 2716 3083 3468 3485 4937 5023 5035 5381 Size: 744 367 385 17 1452 86 12 346 Cuts at: 5381 1972 Size: 1977 Fragments arranged by size: 1977 1452 744 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2235 2235 Size: 5386 BtgI C'CryG_G Cuts at: 513 1065 2682 513 Size: 552 1617 3217 Fragments arranged by size: 3217 1617 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4647 Fragments arranged by size: 4647 683 56 BtsI GCAGTG_nn' Cuts at: 2141 2902 3696 3716 2141 Size: 761 794 20 3811 Fragments arranged by size: 3811 794 761 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5386 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5351 DraI TTT'AAA Cuts at: 2690 2690 Size: 5386 DraIII CAC_nnn'GTG Cuts at: 2287 2287 Size: 5386 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2402 4393 809 Size: 507 1086 1991 1802 Fragments arranged by size: 1991 1802 1086 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2013 2157 2833 Size: 54 1425 50 275 18 183 144 676 Cuts at: 2833 3722 5351 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 676 275 183 144 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2013 2833 1537 Size: 275 201 820 4090 Fragments arranged by size: 4090 820 275 201 EarI CTCTTCn'nnn_ Cuts at: 1353 3316 1353 Size: 1963 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3973 4347 4493 387 Size: 1064 138 2384 374 146 1280 Fragments arranged by size: 2384 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2246 2320 2372 2519 3449 4046 4833 Size: 393 614 74 52 147 930 597 787 Cuts at: 4833 5101 1239 Size: 268 1524 Fragments arranged by size: 1524 930 787 614 597 393 268 147 74 52 EcoNI CCTnn'n_nnAGG Cuts at: 2561 2561 Size: 5386 EcoO109I rG'GnC_Cy Cuts at: 2078 2079 2078 Size: 1 5385 EcoRI G'AATT_C Cuts at: 2695 3073 2695 Size: 378 5008 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2338 2674 2338 Size: 336 5050 FspI TGC'GCA Cuts at: 3892 3892 Size: 5386 HaeII r_GCGC'y Cuts at: 1678 2656 2674 4533 1678 Size: 978 18 1859 2531 Fragments arranged by size: 2531 1859 978 18 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4027 4059 4101 4133 1373 Size: 32 135 32 2455 32 42 32 2626 Fragments arranged by size: 2626 2455 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2584 2602 2730 2976 669 Size: 988 281 646 18 128 246 3079 Fragments arranged by size: 3079 988 646 281 246 128 18 HindIII A'AGCT_T Cuts at: 748 748 Size: 5386 HpaI GTT'AAC Cuts at: 2976 2976 Size: 5386 KpnI G_GTAC'C Cuts at: 2711 2711 Size: 5386 MfeI C'AATT_G Cuts at: 2985 2985 Size: 5386 MluI A'CGCG_T Cuts at: 5262 5262 Size: 5386 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4500 4684 1305 Size: 3195 184 2007 Fragments arranged by size: 3195 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3564 Fragments arranged by size: 3564 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2513 3344 3703 3862 518 Size: 648 1347 831 359 159 2042 Fragments arranged by size: 2042 1347 831 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2597 2802 3479 4627 4872 1606 Size: 991 205 677 1148 245 2120 Fragments arranged by size: 2120 1148 991 677 245 205 NaeI GCC'GGC Cuts at: 2017 2121 2157 2017 Size: 104 36 5246 Fragments arranged by size: 5246 104 36 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4834 NdeI CA'TA_TG Cuts at: 387 387 Size: 5386 NgoMIV G'CCGG_C Cuts at: 2015 2119 2155 2015 Size: 104 36 5246 Fragments arranged by size: 5246 104 36 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5386 NotI GC'GGCC_GC Cuts at: 2833 2833 Size: 5386 NspI r_CATG'y Cuts at: 1132 1229 2744 4289 1132 Size: 97 1515 1545 2229 Fragments arranged by size: 2229 1545 1515 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5386 PciI A'CATG_T Cuts at: 4285 4285 Size: 5386 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5386 PfoI T'CCnGG_A Cuts at: 2091 2388 2661 2091 Size: 297 273 4816 Fragments arranged by size: 4816 297 273 PmeI GTTT'AAAC Cuts at: 2690 2690 Size: 5386 PmlI CAC'GTG Cuts at: 2546 2546 Size: 5386 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2093 2125 3455 3487 4968 5000 2093 Size: 32 1330 32 1481 32 2479 Fragments arranged by size: 2479 1481 1330 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5386 PsiI TTA'TAA Cuts at: 2956 2956 Size: 5386 PspOMI G'GGCC_C Cuts at: 2078 2078 Size: 5386 PstI C_TGCA'G Cuts at: 830 2637 2738 830 Size: 1807 101 3478 Fragments arranged by size: 3478 1807 101 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5386 PvuII CAG'CTG Cuts at: 2597 2597 Size: 5386 SalI G'TCGA_C Cuts at: 1936 2728 1936 Size: 792 4594 SbfI CC_TGCA'GG Cuts at: 2738 2738 Size: 5386 ScaI AGT'ACT Cuts at: 3634 5346 3634 Size: 1712 3674 SexAI A'CCwGG_T Cuts at: 2184 2184 Size: 5386 SfcI C'TryA_G Cuts at: 826 1045 2633 2734 3869 4550 4741 826 Size: 219 1588 101 1135 681 191 1471 Fragments arranged by size: 1588 1471 1135 681 219 191 101 SfiI GGCCn_nnn'nGGCC Cuts at: 2021 2021 Size: 5386 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5386 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5386 SmaI CCC'GGG Cuts at: 1872 2713 1872 Size: 841 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2171 2648 3490 4391 4653 820 Size: 197 926 228 477 842 901 262 1553 Fragments arranged by size: 1553 926 901 842 477 262 228 197 SnaBI TAC'GTA Cuts at: 493 3177 493 Size: 2684 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5386 SphI G_CATG'C Cuts at: 1132 2744 1132 Size: 1612 3774 SspI AAT'ATT Cuts at: 5 52 3310 5 Size: 47 3258 2081 Fragments arranged by size: 3258 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5386 StyI C'CwwG_G Cuts at: 513 1065 2825 513 Size: 552 1760 3074 Fragments arranged by size: 3074 1760 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2192 3413 3430 3583 3768 4213 325 Size: 1255 612 1221 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1221 612 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3632 5344 96 Size: 274 80 33 51 1443 1655 1712 138 Fragments arranged by size: 1712 1655 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2196 2228 5346 5378 2196 Size: 32 3118 32 2204 Fragments arranged by size: 3118 2204 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5386 XbaI T'CTAG_A Cuts at: 2722 2722 Size: 5386 XhoI C'TCGA_G Cuts at: 1943 2171 1943 Size: 228 5158 XmaI C'CCGG_G Cuts at: 1870 2711 1870 Size: 841 4545 XmnI GAAnn'nnTTC Cuts at: 3515 3515 Size: 5386 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3194 276 Size: 53 83 186 936 1660 2468 Fragments arranged by size: 2468 1660 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoICRI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SfoI SrfI SwaI XcmI