(Circular) (Six-base) MAPSORT of: pFN21AB3580.seq Check: 4834 from: 1 to: 6319 pFN21AB3580 6319 bp With 182 enzymes: SgfI * October 20, 2009 10:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4129 278 Size: 53 83 186 936 2593 2468 Fragments arranged by size: 2593 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3662 1937 Size: 1725 4594 Acc65I G'GTAC_C Cuts at: 3640 3640 Size: 6319 AclI AA'CG_TT Cuts at: 1563 4446 4819 1563 Size: 2883 373 3063 Fragments arranged by size: 3063 2883 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2454 2468 4382 5766 6034 1632 Size: 822 14 1914 1384 268 1917 Fragments arranged by size: 1917 1914 1384 822 268 14 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6122 AflIII A'CryG_T Cuts at: 1203 5218 6195 1203 Size: 4015 977 1327 Fragments arranged by size: 4015 1327 977 AgeI A'CCGG_T Cuts at: 5150 5150 Size: 6319 AhdI GACnn_n'nnGTC Cuts at: 5048 5048 Size: 6319 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6319 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6319 AlwNI CAG_nnn'CTG Cuts at: 2513 4062 5634 2513 Size: 1549 1572 3198 Fragments arranged by size: 3198 1572 1549 ApaI G_GGCC'C Cuts at: 2836 2836 Size: 6319 ApaLI G'TGCA_C Cuts at: 4376 5532 4376 Size: 1156 5163 ApoI r'AATT_y Cuts at: 1493 2318 2476 2747 2868 3182 3628 3854 4006 Size: 825 158 271 121 314 446 226 152 Cuts at: 4006 6115 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 825 446 314 271 226 158 152 121 AseI AT'TA_AT Cuts at: 160 1032 2220 4873 160 Size: 872 1188 2653 1606 Fragments arranged by size: 2653 1606 1188 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6319 AvaI C'yCGr_G Cuts at: 1870 1943 3644 1870 Size: 73 1701 4545 Fragments arranged by size: 4545 1701 73 BamHI G'GATC_C Cuts at: 3649 4016 3649 Size: 367 5952 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2236 3640 5095 618 Size: 325 217 627 449 1404 1455 1842 Fragments arranged by size: 1842 1455 1404 627 449 325 217 BanII G_rGCy'C Cuts at: 1391 2581 2836 3638 1391 Size: 1190 255 802 4072 Fragments arranged by size: 4072 1190 802 255 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3537 928 Size: 971 1638 3710 Fragments arranged by size: 3710 1638 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4510 4544 1903 Size: 34 2573 34 3678 Fragments arranged by size: 3678 2573 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4211 5421 4211 Size: 1210 5109 BclI T'GATC_A Cuts at: 1548 1548 Size: 6319 BfrBI ATG'CAT Cuts at: 2923 2923 Size: 6319 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2831 4930 136 Size: 107 122 71 631 605 1159 2099 1525 Fragments arranged by size: 2099 1525 1159 631 605 122 107 71 BglII A'GATC_T Cuts at: 3477 6314 3477 Size: 2837 3482 BlpI GC'TnA_GC Cuts at: 2537 3016 3736 2537 Size: 479 720 5120 Fragments arranged by size: 5120 720 479 Bme1580I G_kGCm'C Cuts at: 1790 2520 2836 3118 4380 5536 1790 Size: 730 316 282 1262 1156 2573 Fragments arranged by size: 2573 1262 1156 730 316 282 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6319 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3278 4050 5008 Size: 416 53 83 399 24 1861 772 958 Cuts at: 5008 442 Size: 1753 Fragments arranged by size: 1861 1753 958 772 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6319 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2616 2727 3252 4979 1239 Size: 1377 111 525 1727 2579 Fragments arranged by size: 2579 1727 1377 525 111 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4444 5309 5607 4444 Size: 865 298 5156 Fragments arranged by size: 5156 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4982 882 Size: 617 3483 2219 Fragments arranged by size: 3483 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4110 493 Size: 711 2906 2702 Fragments arranged by size: 2906 2702 711 BsaBI GATnn'nnATC Cuts at: 6313 6313 Size: 6319 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4126 4508 5143 275 Size: 53 83 186 936 2593 382 635 1451 Fragments arranged by size: 2593 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4752 5150 5424 5571 5982 4752 Size: 398 274 147 411 5089 Fragments arranged by size: 5089 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6094 Fragments arranged by size: 6094 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2098 3287 1186 Size: 912 1189 4218 Fragments arranged by size: 4218 1189 912 BseYI C'CCAG_C Cuts at: 2829 3151 5522 6018 2829 Size: 322 2371 496 3130 Fragments arranged by size: 3130 2371 496 322 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2632 1163 Size: 573 896 4850 Fragments arranged by size: 4850 896 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3769 4530 5558 1540 Size: 275 186 1768 761 1028 2301 Fragments arranged by size: 2301 1768 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4380 4465 5536 4380 Size: 85 1071 5163 Fragments arranged by size: 5163 1071 85 BsmI GAATG_Cn' Cuts at: 2701 3830 3923 2701 Size: 1129 93 5097 Fragments arranged by size: 5097 1129 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2520 2581 2836 3118 3638 4380 4465 Size: 399 730 61 255 282 520 742 85 Cuts at: 4465 5536 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 730 520 399 282 255 85 61 BspEI T'CCGG_A Cuts at: 5982 5982 Size: 6319 BspHI T'CATG_A Cuts at: 2206 3463 4208 5938 2206 Size: 1257 745 1730 2587 Fragments arranged by size: 2587 1730 1257 745 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3674 844 Size: 2830 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3286 4206 1394 Size: 429 1463 920 3507 Fragments arranged by size: 3507 1463 920 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2589 3540 4814 4988 65 Size: 1183 1341 951 1274 174 1396 Fragments arranged by size: 1396 1341 1274 1183 951 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4963 5150 1700 Size: 110 3153 187 2869 Fragments arranged by size: 3153 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2426 3322 96 Size: 2330 896 3093 Fragments arranged by size: 3093 2330 896 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6319 BssSI C'ACGA_G Cuts at: 4379 5391 4379 Size: 1012 5307 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6319 BstBI TT'CG_AA Cuts at: 5123 5123 Size: 6319 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6319 BstXI CCAn_nnnn'nTGG Cuts at: 2398 2398 Size: 6319 BstYI r'GATC_y Cuts at: 1972 3120 3477 3649 4016 4401 4418 5870 5956 Size: 1148 357 172 367 385 17 1452 86 Cuts at: 5956 5968 6314 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1148 385 367 357 346 172 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3356 3356 Size: 6319 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5767 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5580 Fragments arranged by size: 5580 683 56 BtsI GCAGTG_nn' Cuts at: 2429 3835 4629 4649 2429 Size: 1406 794 20 4099 Fragments arranged by size: 4099 1406 794 20 ClaI AT'CG_AT Cuts at: 1554 2883 1554 Size: 1329 4990 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6284 DraI TTT'AAA Cuts at: 3623 3623 Size: 6319 DraIII CAC_nnn'GTG Cuts at: 2243 2243 Size: 6319 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5326 809 Size: 507 4010 1802 Fragments arranged by size: 4010 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2388 3766 4655 Size: 54 1425 50 275 18 558 1378 889 Cuts at: 4655 6284 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1378 889 558 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3766 1537 Size: 275 1954 4090 Fragments arranged by size: 4090 1954 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2121 2487 4249 1353 Size: 768 366 1762 3423 Fragments arranged by size: 3423 1762 768 366 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4906 5280 5426 387 Size: 1064 138 3317 374 146 1280 Fragments arranged by size: 3317 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2454 2468 2616 2727 3252 4382 4979 Size: 393 822 14 148 111 525 1130 597 Cuts at: 4979 5766 6034 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1130 822 787 597 525 393 268 148 111 14 EcoNI CCTnn'n_nnAGG Cuts at: 3603 3603 Size: 6319 EcoO109I rG'GnC_Cy Cuts at: 2833 2833 Size: 6319 EcoRI G'AATT_C Cuts at: 3628 4006 3628 Size: 378 5941 EcoRV GAT'ATC Cuts at: 3090 3090 Size: 6319 FspI TGC'GCA Cuts at: 2078 4825 2078 Size: 2747 3572 HaeII r_GCGC'y Cuts at: 1678 5466 1678 Size: 3788 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4960 4992 5034 5066 1373 Size: 32 135 32 3388 32 42 32 2626 Fragments arranged by size: 3388 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3556 3663 3909 669 Size: 988 281 1618 107 246 3079 Fragments arranged by size: 3079 1618 988 281 246 107 HindIII A'AGCT_T Cuts at: 748 3426 748 Size: 2678 3641 HpaI GTT'AAC Cuts at: 3909 3909 Size: 6319 KpnI G_GTAC'C Cuts at: 3644 3644 Size: 6319 MfeI C'AATT_G Cuts at: 3918 3918 Size: 6319 MluI A'CGCG_T Cuts at: 6195 6195 Size: 6319 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3062 3607 5433 5617 1305 Size: 1757 545 1826 184 2007 Fragments arranged by size: 2007 1826 1757 545 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2390 10 Size: 54 1425 343 558 3939 Fragments arranged by size: 3939 1425 558 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2231 3305 4277 4636 4795 518 Size: 648 1065 1074 972 359 159 2042 Fragments arranged by size: 2042 1074 1065 972 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3104 3242 3735 4412 5560 5805 1606 Size: 1498 138 493 677 1148 245 2120 Fragments arranged by size: 2120 1498 1148 677 493 245 138 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5767 NdeI CA'TA_TG Cuts at: 387 387 Size: 6319 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6319 NotI GC'GGCC_GC Cuts at: 3766 3766 Size: 6319 NsiI A_TGCA'T Cuts at: 2925 2925 Size: 6319 NspI r_CATG'y Cuts at: 1132 1229 3081 3677 5222 1132 Size: 97 1852 596 1545 2229 Fragments arranged by size: 2229 1852 1545 596 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6319 PciI A'CATG_T Cuts at: 5218 5218 Size: 6319 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6319 PfoI T'CCnGG_A Cuts at: 2559 2559 Size: 6319 PmeI GTTT'AAAC Cuts at: 3623 3623 Size: 6319 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4388 4420 5901 5933 4388 Size: 32 1481 32 4774 Fragments arranged by size: 4774 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6319 PsiI TTA'TAA Cuts at: 2934 3889 2934 Size: 955 5364 PspOMI G'GGCC_C Cuts at: 2832 2832 Size: 6319 PstI C_TGCA'G Cuts at: 830 2106 2436 3027 3671 830 Size: 1276 330 591 644 3478 Fragments arranged by size: 3478 1276 644 591 330 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6319 PvuII CAG'CTG Cuts at: 3104 3242 3104 Size: 138 6181 SalI G'TCGA_C Cuts at: 1936 3661 1936 Size: 1725 4594 SapI GCTCTTCn'nnn_ Cuts at: 2121 2121 Size: 6319 SbfI CC_TGCA'GG Cuts at: 3671 3671 Size: 6319 ScaI AGT'ACT Cuts at: 4567 6279 4567 Size: 1712 4607 SexAI A'CCwGG_T Cuts at: 2239 2239 Size: 6319 SfcI C'TryA_G Cuts at: 826 1045 2102 2157 2432 2722 3023 3667 4802 Size: 219 1057 55 275 290 301 644 1135 Cuts at: 4802 5483 5674 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1057 681 644 301 290 275 219 191 55 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6319 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6319 SmaI CCC'GGG Cuts at: 1872 3646 1872 Size: 1774 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4423 5324 5586 820 Size: 197 926 2480 901 262 1553 Fragments arranged by size: 2480 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4110 493 Size: 3617 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6319 SphI G_CATG'C Cuts at: 1132 3677 1132 Size: 2545 3774 SspI AAT'ATT Cuts at: 5 52 2464 3097 4243 5 Size: 47 2412 633 1146 2081 Fragments arranged by size: 2412 2081 1146 633 47 StuI AGG'CCT Cuts at: 1517 3491 1517 Size: 1974 4345 StyI C'CwwG_G Cuts at: 513 1065 3758 513 Size: 552 2693 3074 Fragments arranged by size: 3074 2693 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3536 4346 4363 4516 4701 5146 325 Size: 1255 1956 810 17 153 185 445 1498 Fragments arranged by size: 1956 1498 1255 810 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2426 2812 3322 Size: 274 80 33 51 1443 449 386 510 Cuts at: 3322 3573 4565 6277 96 Size: 251 992 1712 138 Fragments arranged by size: 1712 1443 992 510 449 386 274 251 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6279 6311 6279 Size: 32 6287 Tth111I GACn'n_nGTC Cuts at: 1316 2621 1316 Size: 1305 5014 XbaI T'CTAG_A Cuts at: 3655 3655 Size: 6319 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6319 XmaI C'CCGG_G Cuts at: 1870 3644 1870 Size: 1774 4545 XmnI GAAnn'nnTTC Cuts at: 4448 4448 Size: 6319 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4127 276 Size: 53 83 186 936 2593 2468 Fragments arranged by size: 2593 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I EcoICRI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI XcmI