################################## # NO CUT ENZYMES ( 116 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AflII AflIII AgeI AhdI ApoI AscI AseI AsnI Asp718I AspEI AspI AviII BalI BamHI BbrPI BbsI BciVI BclI BfrI BglII BpuAI BsaBI BsaWI BseAI BseRI BsgI BsiEI BsiWI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrGI BssHII BstAPI BstBI BstEII BstF5I BstPI BstUI ClaI DraI DrdI EagI Eam1105I EclXI Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FokI FseI FspI HincII HindII HindIII HpaI KpnI KspI MamI MfeI MluI MluNI MroI MscI MunI MvnI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmlI SnaBI Sse8387I SspBI SspI SwaI Tth111I XhoI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### AccI 1263 GTATAC GT^[AC][GT]AC AfaI 960 GTAC GT^AC Alw44I 436 GTGCAC G^TGCAC AlwI 1018 GATCC GGATC(4/5) Aor51HI 665 AGCGCT AGC^GCT ApaLI 436 GTGCAC G^TGCAC Asp700I 285 GAACAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvrII 198 CCTAGG C^CTAGG BbeI 1388 GGCGCC GGCGC^C BcgI 573 GCAAACCCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlnI 198 CCTAGG C^CTAGG BsaAI 298 TACGTG [CT]AC^GT[GA] BsaI 266 GAGACC GGTCTC(1/5) BsmBI 476 CGTCTC CGTCTC(1/5) BsmFI 818 GGGAC GGGAC(10/14) BsmI 1253 GCATTC GAATGC(1/-1) BsrFI 765 GCCGGT [GA]^CCGG[CT] BssSI 908 CTCGTG CACGAG(-5/-1) Bst1107I 1263 GTATAC GTA^TAC BstYI 1017 AGATCC [GA]^GATC[CT] BstZ17I 1263 GTATAC GTA^TAC Cfr10I 765 GCCGGT [GA]^CCGG[CT] CpoI 7 CGGACCG CG^G[AT]CCG DpnII 1018 GATC ^GATC DraIII 683 CACTTCGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 44 TGGCCG [CT]^GGCC[GA] EarI 559 CTCTTC CTCTTC(1/4) Eco47III 665 AGCGCT AGC^GCT KasI 1388 GGCGCC G^GCGCC Ksp632I 559 CTCTTC CTCTTC(1/4) MboI 1018 GATC ^GATC MflI 1017 AGATCC [GA]^GATC[CT] MseI 1367 TTAA T^TAA MslI 927 CACTGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NarI 1388 GGCGCC GG^CGCC NcoI 485 CCATGG C^CATGG NdeII 1018 GATC ^GATC NspI 315 GCATGC [GA]CATG^[CT] PpuMI 541 AGGTCCT [GA]G^G[AT]CC[CT] RsaI 960 GTAC GT^AC RsrII 7 CGGACCG CG^G[AT]CCG SacI 529 GAGCTC GAGCT^C Sau3AI 1018 GATC ^GATC SfoI 1388 GGCGCC GGC^GCC SmaI 410 CCCGGG CCC^GGG SpeI 733 ACTAGT A^CTAGT SphI 315 GCATGC GCATG^C StuI 653 AGGCCT AGG^CCT TaqI 138 TCGA T^CGA Tru9I 1367 TTAA T^TAA TthHB8I 138 TCGA T^CGA XbaI 696 TCTAGA T^CTAGA XhoII 1017 AGATCC [GA]^GATC[CT] XmaCI 410 CCCGGG C^CCGGG XmaI 410 CCCGGG C^CCGGG XmnI 285 GAACAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AcyI 474 GGCGTC G[GA]^CG[CT]C 1388 GGCGCC AvaI 173 CTCGGG C^[CT]CG[GA]G 410 CCCGGG BbvCI 390 GCTGAGG CCTCAGC(-5/-2) 889 GCTGAGG BsaHI 474 GGCGTC G[GA]^CG[CT]C 1388 GGCGCC BsoBI 173 CTCGGG C^[CT]CG[GA]G 410 CCCGGG BstXI 587 CCATATTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 984 CCAGGGATTTGG FauI 190 GCGGG CCCGC(4/6) 1173 GCGGG HgaI 111 GCGTC GACGC(5/10) 475 GCGTC Hin1I 474 GGCGTC G[GA]^CG[CT]C 1388 GGCGCC HphI 948 GGTGA GGTGA(8/7) 355 TCACC MaeII 299 ACGT A^CGT 842 ACGT NlaIII 316 CATG CATG^ 486 CATG PflMI 651 CCAGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1224 CCACTGCTTGG PshAI 9 GACCGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 820 GACAGCAGTC PstI 929 CTGCAG CTGCA^G 955 CTGCAG PvuII 275 CAGCTG CAG^CTG 1187 CAGCTG Van91I 651 CCAGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1224 CCACTGCTTGG XcmI 450 CCAGCGCCGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 762 CCAGCCGGTCTGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 68 enzymes) # ######################################### AciI AluI AlwNI AocI ApaI AspHI AvaII BanI BanII BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BsaJI BsiHKAI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstNI Bsu36I Cac8I CelII CfoI Cfr13I DdeI DraII DsaI Eco81I EcoO109I EcoRII EcoT14I Fnu4HI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII ItaI MaeI MaeIII MboII MnlI MspA1I MspI MvaI MwoI NciI NlaIV PleI Sau96I ScrFI SfaNI SfcI StyI TfiI TseI Tsp45I Tsp509I TspRI