(Circular) (Six-base) MAPSORT of: pFN21AE4461.seq Check: 8640 from: 1 to: 6136 pFN21AE4461 6136 bp With 182 enzymes: SgfI * March 28, 2012 13:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3946 278 Size: 53 83 186 936 2410 2468 Fragments arranged by size: 2468 2410 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3269 3479 1937 Size: 1332 210 4594 Fragments arranged by size: 4594 1332 210 Acc65I G'GTAC_C Cuts at: 3457 3457 Size: 6136 AclI AA'CG_TT Cuts at: 1563 4263 4636 1563 Size: 2700 373 3063 Fragments arranged by size: 3063 2700 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2853 3000 3233 4199 5583 5851 1632 Size: 1221 147 233 966 1384 268 1917 Fragments arranged by size: 1917 1384 1221 966 268 233 147 AfeI AGC'GCT Cuts at: 2672 2672 Size: 6136 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5939 AflIII A'CryG_T Cuts at: 1203 5035 6012 1203 Size: 3832 977 1327 Fragments arranged by size: 3832 1327 977 AgeI A'CCGG_T Cuts at: 4967 4967 Size: 6136 AhdI GACnn_n'nnGTC Cuts at: 4865 4865 Size: 6136 AleI CACnn'nnGTG Cuts at: 1166 2936 1166 Size: 1770 4366 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2466 1184 Size: 1282 4854 AlwNI CAG_nnn'CTG Cuts at: 2502 2550 2942 3133 3166 3879 5451 2502 Size: 48 392 191 33 713 1572 3187 Fragments arranged by size: 3187 1572 713 392 191 48 33 ApaI G_GGCC'C Cuts at: 2077 2212 3184 2077 Size: 135 972 5029 Fragments arranged by size: 5029 972 135 ApaLI G'TGCA_C Cuts at: 2441 4193 5349 2441 Size: 1752 1156 3228 Fragments arranged by size: 3228 1752 1156 ApoI r'AATT_y Cuts at: 1493 3445 3671 3823 5932 1493 Size: 1952 226 152 2109 1697 Fragments arranged by size: 2109 1952 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4690 160 Size: 872 3658 1606 Fragments arranged by size: 3658 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6136 AvaI C'yCGr_G Cuts at: 1870 1943 2178 2415 3461 1870 Size: 73 235 237 1046 4545 Fragments arranged by size: 4545 1046 237 235 73 AvrII C'CTAG_G Cuts at: 2203 2203 Size: 6136 BamHI G'GATC_C Cuts at: 3466 3833 3466 Size: 367 5769 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2312 3007 3393 3457 4912 Size: 325 217 627 525 695 386 64 1455 Cuts at: 4912 618 Size: 1842 Fragments arranged by size: 1842 1455 695 627 525 386 325 217 64 BanII G_rGCy'C Cuts at: 1391 2077 2203 2212 2538 2784 2977 3083 3184 Size: 686 126 9 326 246 193 106 101 Cuts at: 3184 3455 1391 Size: 271 4072 Fragments arranged by size: 4072 686 326 271 246 193 126 106 101 9 BbeI G_GCGC'C Cuts at: 3397 3397 Size: 6136 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5165 BbvCI CC'TCA_GC Cuts at: 2396 2895 2396 Size: 499 5637 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2567 2601 4327 4361 1903 Size: 34 630 34 1726 34 3678 Fragments arranged by size: 3678 1726 630 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4028 5238 4028 Size: 1210 4926 BclI T'GATC_A Cuts at: 1548 1548 Size: 6136 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2207 2909 3087 Size: 107 122 71 631 605 535 702 178 Cuts at: 3087 4747 136 Size: 1660 1525 Fragments arranged by size: 1660 1525 702 631 605 535 178 122 107 71 BglII A'GATC_T Cuts at: 6131 6131 Size: 6136 BlpI GC'TnA_GC Cuts at: 2537 2574 2667 2783 3553 2537 Size: 37 93 116 770 5120 Fragments arranged by size: 5120 770 116 93 37 Bme1580I G_kGCm'C Cuts at: 1790 2077 2212 2317 2445 2920 3184 3422 4197 Size: 287 135 105 128 475 264 238 775 Cuts at: 4197 5353 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 775 475 287 264 238 135 128 105 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6136 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2553 2609 3259 Size: 416 53 83 399 24 1136 56 650 Cuts at: 3259 3867 4825 442 Size: 608 958 1753 Fragments arranged by size: 1753 1136 958 650 608 416 399 83 56 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6136 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2695 2774 2855 3155 3366 4796 1239 Size: 1456 79 81 300 211 1430 2579 Fragments arranged by size: 2579 1456 1430 300 211 81 79 Bpu10I CC'TnA_GC Cuts at: 2066 2396 2895 3066 2066 Size: 330 499 171 5136 Fragments arranged by size: 5136 499 330 171 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4261 5126 5424 4261 Size: 865 298 4973 Fragments arranged by size: 4973 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2265 4799 882 Size: 617 766 2534 2219 Fragments arranged by size: 2534 2219 766 617 BsaAI yAC'GTr Cuts at: 493 1204 2305 3927 493 Size: 711 1101 1622 2702 Fragments arranged by size: 2702 1622 1101 711 BsaBI GATnn'nnATC Cuts at: 6130 6130 Size: 6136 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2480 3394 3943 4325 Size: 53 83 186 936 947 914 549 382 Cuts at: 4325 4960 275 Size: 635 1451 Fragments arranged by size: 1451 947 936 914 635 549 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4569 4967 5241 5388 5799 4569 Size: 398 274 147 411 4906 Fragments arranged by size: 4906 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3354 3384 1178 Size: 30 165 30 1951 30 3930 Fragments arranged by size: 3930 1951 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6136 BseYI C'CCAG_C Cuts at: 2026 2278 2390 2454 2611 3109 5339 5835 2026 Size: 252 112 64 157 498 2230 496 2327 Fragments arranged by size: 2327 2230 498 496 252 157 112 64 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5563 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3586 4347 5375 1540 Size: 275 186 1585 761 1028 2301 Fragments arranged by size: 2301 1585 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2445 2538 2877 4197 4282 5353 2445 Size: 93 339 1320 85 1071 3228 Fragments arranged by size: 3228 1320 1071 339 93 85 BsmI GAATG_Cn' Cuts at: 3258 3647 3740 3258 Size: 389 93 5654 Fragments arranged by size: 5654 389 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2487 2487 Size: 6136 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2077 2203 2212 2317 2445 2538 2784 Size: 399 287 126 9 105 128 93 246 Cuts at: 2784 2877 2920 2977 3083 3184 3422 3455 4197 Size: 93 43 57 106 101 238 33 742 Cuts at: 4197 4282 5353 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 287 246 238 128 126 106 105 101 93 93 85 57 43 33 9 BspEI T'CCGG_A Cuts at: 5799 5799 Size: 6136 BspHI T'CATG_A Cuts at: 4025 5755 4025 Size: 1730 4406 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3491 844 Size: 2647 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4023 1394 Size: 429 2200 3507 Fragments arranged by size: 3507 2200 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4631 4805 65 Size: 1183 3383 174 1396 Fragments arranged by size: 3383 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2770 4780 4967 1700 Size: 110 960 2010 187 2869 Fragments arranged by size: 2869 2010 960 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6136 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6136 BssSI C'ACGA_G Cuts at: 2913 4196 5208 2913 Size: 1283 1012 3841 Fragments arranged by size: 3841 1283 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6136 BstBI TT'CG_AA Cuts at: 4940 4940 Size: 6136 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6136 BstXI CCAn_nnnn'nTGG Cuts at: 2599 2996 2599 Size: 397 5739 BstYI r'GATC_y Cuts at: 1972 3022 3466 3833 4218 4235 5687 5773 5785 Size: 1050 444 367 385 17 1452 86 12 Cuts at: 5785 6131 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1050 444 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 3270 3270 Size: 6136 Bsu36I CC'TnA_GG Cuts at: 2088 2339 2472 2715 2088 Size: 251 133 243 5509 Fragments arranged by size: 5509 251 243 133 BtgI C'CryG_G Cuts at: 513 1065 2004 2016 2490 2506 513 Size: 552 939 12 474 16 4143 Fragments arranged by size: 4143 939 552 474 16 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5397 Fragments arranged by size: 5397 683 56 BtsI GCAGTG_nn' Cuts at: 2116 2931 2943 3229 3652 4446 4466 2116 Size: 815 12 286 423 794 20 3786 Fragments arranged by size: 3786 815 794 423 286 20 12 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6136 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6101 DraI TTT'AAA Cuts at: 3440 3440 Size: 6136 DraIII CAC_nnn'GTG Cuts at: 2693 2693 Size: 6136 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5143 809 Size: 507 3827 1802 Fragments arranged by size: 3827 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2049 3583 4472 Size: 54 1425 50 275 18 219 1534 889 Cuts at: 4472 6101 8 Size: 1629 43 Fragments arranged by size: 1629 1534 1425 889 275 219 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3583 1537 Size: 275 1771 4090 Fragments arranged by size: 4090 1771 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2570 4066 1353 Size: 1217 1496 3423 Fragments arranged by size: 3423 1496 1217 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2046 2342 2638 4723 5097 5243 Size: 1064 138 457 296 296 2085 374 146 Cuts at: 5243 387 Size: 1280 Fragments arranged by size: 2085 1280 1064 457 374 296 296 146 138 EcoICRI GAG'CTC Cuts at: 2536 2536 Size: 6136 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2695 2774 2853 2855 3000 3155 3233 Size: 393 1063 79 79 2 145 155 78 Cuts at: 3233 3366 4199 4796 5583 5851 1239 Size: 133 833 597 787 268 1524 Fragments arranged by size: 1524 1063 833 787 597 393 268 155 145 133 79 79 78 2 EcoO109I rG'GnC_Cy Cuts at: 2074 2147 2208 2547 2719 2074 Size: 73 61 339 172 5491 Fragments arranged by size: 5491 339 172 73 61 EcoRI G'AATT_C Cuts at: 3445 3823 3445 Size: 378 5758 FspI TGC'GCA Cuts at: 4642 4642 Size: 6136 HaeII r_GCGC'y Cuts at: 1678 2461 2674 2806 3397 5283 1678 Size: 783 213 132 591 1886 2531 Fragments arranged by size: 2531 1886 783 591 213 132 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2465 2497 2698 2730 4777 Size: 32 135 32 893 32 201 32 2047 Cuts at: 4777 4809 4851 4883 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2047 893 201 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3480 3726 669 Size: 988 281 1542 246 3079 Fragments arranged by size: 3079 1542 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6136 HpaI GTT'AAC Cuts at: 3726 3726 Size: 6136 KasI G'GCGC_C Cuts at: 3393 3393 Size: 6136 KpnI G_GTAC'C Cuts at: 3461 3461 Size: 6136 MfeI C'AATT_G Cuts at: 3735 3735 Size: 6136 MluI A'CGCG_T Cuts at: 6012 6012 Size: 6136 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5250 5434 1305 Size: 3945 184 2007 Fragments arranged by size: 3945 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4314 Fragments arranged by size: 4314 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2936 4094 4453 4612 518 Size: 648 1770 1158 359 159 2042 Fragments arranged by size: 2042 1770 1158 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2043 2195 2282 3178 3194 3552 4229 5377 Size: 437 152 87 896 16 358 677 1148 Cuts at: 5377 5622 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 896 677 437 358 245 152 87 16 NarI GG'CG_CC Cuts at: 3394 3394 Size: 6136 NcoI C'CATG_G Cuts at: 513 1065 2004 2490 513 Size: 552 939 486 4159 Fragments arranged by size: 4159 939 552 486 NdeI CA'TA_TG Cuts at: 387 387 Size: 6136 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6136 NotI GC'GGCC_GC Cuts at: 3583 3583 Size: 6136 NspI r_CATG'y Cuts at: 1132 1229 2324 3494 5039 1132 Size: 97 1095 1170 1545 2229 Fragments arranged by size: 2229 1545 1170 1095 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6136 PciI A'CATG_T Cuts at: 5035 5035 Size: 6136 PflMI CCAn_nnn'nTGG Cuts at: 1108 2662 3235 1108 Size: 1554 573 4009 Fragments arranged by size: 4009 1554 573 PfoI T'CCnGG_A Cuts at: 3025 3025 Size: 6136 PmeI GTTT'AAAC Cuts at: 3440 3440 Size: 6136 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4205 4237 5718 5750 4205 Size: 32 1481 32 4591 Fragments arranged by size: 4591 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2547 2547 Size: 6136 PshAI GACnn'nnGTC Cuts at: 1613 2018 2829 1613 Size: 405 811 4920 Fragments arranged by size: 4920 811 405 PsiI TTA'TAA Cuts at: 3706 3706 Size: 6136 PspOMI G'GGCC_C Cuts at: 2073 2208 3180 2073 Size: 135 972 5029 Fragments arranged by size: 5029 972 135 PstI C_TGCA'G Cuts at: 830 2938 2964 3488 830 Size: 2108 26 524 3478 Fragments arranged by size: 3478 2108 524 26 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6136 PvuII CAG'CTG Cuts at: 2282 3194 2282 Size: 912 5224 RsrII CG'GwC_CG Cuts at: 2013 2013 Size: 6136 SacI G_AGCT'C Cuts at: 2538 2538 Size: 6136 SalI G'TCGA_C Cuts at: 1936 3478 1936 Size: 1542 4594 SbfI CC_TGCA'GG Cuts at: 3488 3488 Size: 6136 ScaI AGT'ACT Cuts at: 4384 6096 4384 Size: 1712 4424 SfcI C'TryA_G Cuts at: 826 1045 2934 2943 2960 3484 4619 5300 5491 Size: 219 1889 9 17 524 1135 681 191 Cuts at: 5491 826 Size: 1471 Fragments arranged by size: 1889 1471 1135 681 524 219 191 17 9 SfoI GGC'GCC Cuts at: 3395 3395 Size: 6136 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6136 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6136 SmaI CCC'GGG Cuts at: 1872 2417 3463 1872 Size: 545 1046 4545 Fragments arranged by size: 4545 1046 545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4240 5141 5403 820 Size: 197 926 2297 901 262 1553 Fragments arranged by size: 2297 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3927 493 Size: 3434 2702 SpeI A'CTAG_T Cuts at: 152 2738 152 Size: 2586 3550 SphI G_CATG'C Cuts at: 1132 2324 3494 1132 Size: 1192 1170 3774 Fragments arranged by size: 3774 1192 1170 SspI AAT'ATT Cuts at: 5 52 4060 5 Size: 47 4008 2081 Fragments arranged by size: 4008 2081 47 StuI AGG'CCT Cuts at: 1517 2660 1517 Size: 1143 4993 StyI C'CwwG_G Cuts at: 513 1065 2004 2203 2464 2490 2661 2968 3575 Size: 552 939 199 261 26 171 307 607 Cuts at: 3575 513 Size: 3074 Fragments arranged by size: 3074 939 607 552 307 261 199 171 26 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2292 4163 4180 4333 4518 4963 325 Size: 1255 712 1871 17 153 185 445 1498 Fragments arranged by size: 1871 1498 1255 712 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4382 6094 96 Size: 274 80 33 51 1443 2405 1712 138 Fragments arranged by size: 2405 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2460 2492 2991 3023 6096 6128 2460 Size: 32 499 32 3073 32 2468 Fragments arranged by size: 3073 2468 499 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6136 XbaI T'CTAG_A Cuts at: 2701 3472 2701 Size: 771 5365 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2462 2774 2462 Size: 312 5824 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6136 XmaI C'CCGG_G Cuts at: 1870 2415 3461 1870 Size: 545 1046 4545 Fragments arranged by size: 4545 1046 545 XmnI GAAnn'nnTTC Cuts at: 2294 4265 2294 Size: 1971 4165 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3944 276 Size: 53 83 186 936 2410 2468 Fragments arranged by size: 2468 2410 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI BaeI BfrBI BplI BsiWI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI SacII SanDI SapI SexAI SfiI SrfI SwaI