(Linear) (Six-base) MAPSORT of: KIEE4461.seq Check: 2163 from: 1 to: 1431 KIEE4461.orf With 182 enzymes: SgfI * March 22, 2012 18:06 .. AccI GT'mk_AC Cuts at: 0 1264 1431 Size: 1264 167 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 848 995 1228 1431 Size: 848 147 233 203 Fragments arranged by size: 848 233 203 147 AfeI AGC'GCT Cuts at: 0 667 1431 Size: 667 764 AleI CACnn'nnGTG Cuts at: 0 931 1431 Size: 931 500 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 461 1431 Size: 461 970 AlwNI CAG_nnn'CTG Cuts at: 0 497 545 937 1128 1161 1431 Size: 497 48 392 191 33 270 Fragments arranged by size: 497 392 270 191 48 33 ApaI G_GGCC'C Cuts at: 0 72 207 1179 1431 Size: 72 135 972 252 Fragments arranged by size: 972 252 135 72 ApaLI G'TGCA_C Cuts at: 0 436 1431 Size: 436 995 AvaI C'yCGr_G Cuts at: 0 173 410 1431 Size: 173 237 1021 Fragments arranged by size: 1021 237 173 AvrII C'CTAG_G Cuts at: 0 198 1431 Size: 198 1233 BanI G'GyrC_C Cuts at: 0 307 1002 1388 1431 Size: 307 695 386 43 Fragments arranged by size: 695 386 307 43 BanII G_rGCy'C Cuts at: 0 72 198 207 533 779 972 1078 1179 Size: 72 126 9 326 246 193 106 101 Cuts at: 1179 1431 Size: 252 Fragments arranged by size: 326 252 246 193 126 106 101 72 9 BbeI G_GCGC'C Cuts at: 0 1392 1431 Size: 1392 39 BbvCI CC'TCA_GC Cuts at: 0 391 890 1431 Size: 391 499 541 Fragments arranged by size: 541 499 391 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 562 596 1431 Size: 562 34 835 Fragments arranged by size: 835 562 34 BglI GCCn_nnn'nGGC Cuts at: 0 202 904 1082 1431 Size: 202 702 178 349 Fragments arranged by size: 702 349 202 178 BlpI GC'TnA_GC Cuts at: 0 532 569 662 778 1431 Size: 532 37 93 116 653 Fragments arranged by size: 653 532 116 93 37 Bme1580I G_kGCm'C Cuts at: 0 72 207 312 440 915 1179 1417 1431 Size: 72 135 105 128 475 264 238 14 Fragments arranged by size: 475 264 238 135 128 105 72 14 BmrI ACTGGGnnnn_n' Cuts at: 0 548 604 1254 1431 Size: 548 56 650 177 Fragments arranged by size: 650 548 177 56 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 690 769 850 1150 1361 1431 Size: 690 79 81 300 211 70 Fragments arranged by size: 690 300 211 81 79 70 Bpu10I CC'TnA_GC Cuts at: 0 61 391 890 1061 1431 Size: 61 330 499 171 370 Fragments arranged by size: 499 370 330 171 61 BsaI GGTCTCn'nnnn_ Cuts at: 0 260 1431 Size: 260 1171 BsaAI yAC'GTr Cuts at: 0 300 1431 Size: 300 1131 BsaHI Gr'CG_yC Cuts at: 0 475 1389 1431 Size: 475 914 42 Fragments arranged by size: 914 475 42 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1349 1379 1431 Size: 1349 30 52 Fragments arranged by size: 1349 52 30 BseYI C'CCAG_C Cuts at: 0 21 273 385 449 606 1104 1431 Size: 21 252 112 64 157 498 327 Fragments arranged by size: 498 327 252 157 112 64 21 BsiHKAI G_wGCw'C Cuts at: 0 440 533 872 1431 Size: 440 93 339 559 Fragments arranged by size: 559 440 339 93 BsmI GAATG_Cn' Cuts at: 0 1253 1431 Size: 1253 178 BsmBI CGTCTCn'nnnn_ Cuts at: 0 482 1431 Size: 482 949 Bsp1286I G_dGCh'C Cuts at: 0 72 198 207 312 440 533 779 872 Size: 72 126 9 105 128 93 246 93 Cuts at: 872 915 972 1078 1179 1417 1431 Size: 43 57 106 101 238 14 Fragments arranged by size: 246 238 128 126 106 105 101 93 93 72 57 43 14 9 BsrFI r'CCGG_y Cuts at: 0 765 1431 Size: 765 666 BssSI C'ACGA_G Cuts at: 0 908 1431 Size: 908 523 BstXI CCAn_nnnn'nTGG Cuts at: 0 594 991 1431 Size: 594 397 440 Fragments arranged by size: 594 440 397 BstYI r'GATC_y Cuts at: 0 1017 1431 Size: 1017 414 BstZ17I GTA'TAC Cuts at: 0 1265 1431 Size: 1265 166 Bsu36I CC'TnA_GG Cuts at: 0 83 334 467 710 1431 Size: 83 251 133 243 721 Fragments arranged by size: 721 251 243 133 83 BtgI C'CryG_G Cuts at: 0 11 485 501 1431 Size: 11 474 16 930 Fragments arranged by size: 930 474 16 11 BtsI GCAGTG_nn' Cuts at: 0 111 926 938 1224 1431 Size: 111 815 12 286 207 Fragments arranged by size: 815 286 207 111 12 DraIII CAC_nnn'GTG Cuts at: 0 688 1431 Size: 688 743 EaeI y'GGCC_r Cuts at: 0 44 1431 Size: 44 1387 EarI CTCTTCn'nnn_ Cuts at: 0 565 1431 Size: 565 866 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 41 337 633 1431 Size: 41 296 296 798 Fragments arranged by size: 798 296 296 41 EcoICRI GAG'CTC Cuts at: 0 531 1431 Size: 531 900 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 690 769 848 850 995 1150 1228 1361 Size: 690 79 79 2 145 155 78 133 Cuts at: 1361 1431 Size: 70 Fragments arranged by size: 690 155 145 133 79 79 78 70 2 EcoO109I rG'GnC_Cy Cuts at: 0 69 142 203 542 714 1431 Size: 69 73 61 339 172 717 Fragments arranged by size: 717 339 172 73 69 61 HaeII r_GCGC'y Cuts at: 0 456 669 801 1392 1431 Size: 456 213 132 591 39 Fragments arranged by size: 591 456 213 132 39 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 460 492 693 725 1431 Size: 460 32 201 32 706 Fragments arranged by size: 706 460 201 32 32 KasI G'GCGC_C Cuts at: 0 1388 1431 Size: 1388 43 MslI CAynn'nnrTG Cuts at: 0 931 1431 Size: 931 500 MspA1I CmG'CkG Cuts at: 0 38 190 277 1173 1189 1431 Size: 38 152 87 896 16 242 Fragments arranged by size: 896 242 152 87 38 16 NarI GG'CG_CC Cuts at: 0 1389 1431 Size: 1389 42 NcoI C'CATG_G Cuts at: 0 485 1431 Size: 485 946 NspI r_CATG'y Cuts at: 0 319 1431 Size: 319 1112 PflMI CCAn_nnn'nTGG Cuts at: 0 657 1230 1431 Size: 657 573 201 Fragments arranged by size: 657 573 201 PfoI T'CCnGG_A Cuts at: 0 1020 1431 Size: 1020 411 PpuMI rG'GwC_Cy Cuts at: 0 542 1431 Size: 542 889 PshAI GACnn'nnGTC Cuts at: 0 13 824 1431 Size: 13 811 607 Fragments arranged by size: 811 607 13 PspOMI G'GGCC_C Cuts at: 0 68 203 1175 1431 Size: 68 135 972 256 Fragments arranged by size: 972 256 135 68 PstI C_TGCA'G Cuts at: 0 933 959 1431 Size: 933 26 472 Fragments arranged by size: 933 472 26 PvuII CAG'CTG Cuts at: 0 277 1189 1431 Size: 277 912 242 Fragments arranged by size: 912 277 242 RsrII CG'GwC_CG Cuts at: 0 8 1431 Size: 8 1423 SacI G_AGCT'C Cuts at: 0 533 1431 Size: 533 898 SfcI C'TryA_G Cuts at: 0 929 938 955 1431 Size: 929 9 17 476 Fragments arranged by size: 929 476 17 9 SfoI GGC'GCC Cuts at: 0 1390 1431 Size: 1390 41 SmaI CCC'GGG Cuts at: 0 412 1431 Size: 412 1019 SpeI A'CTAG_T Cuts at: 0 733 1431 Size: 733 698 SphI G_CATG'C Cuts at: 0 319 1431 Size: 319 1112 StuI AGG'CCT Cuts at: 0 655 1431 Size: 655 776 StyI C'CwwG_G Cuts at: 0 198 459 485 656 963 1431 Size: 198 261 26 171 307 468 Fragments arranged by size: 468 307 261 198 171 26 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 287 1431 Size: 287 1144 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 455 487 986 1018 1431 Size: 455 32 499 32 413 Fragments arranged by size: 499 455 413 32 32 XbaI T'CTAG_A Cuts at: 0 696 1431 Size: 696 735 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 457 769 1431 Size: 457 312 662 Fragments arranged by size: 662 457 312 XmaI C'CCGG_G Cuts at: 0 410 1431 Size: 410 1021 XmnI GAAnn'nnTTC Cuts at: 0 289 1431 Size: 289 1142 Enzymes that do cut: AccI AcuI AfeI AleI AlfI AlwNI ApaI ApaLI AvaI AvrII BanI BanII BbeI BbvCI BcgI BglI BlpI Bme1580I BmrI BpmI Bpu10I BsaI BsaAI BsaHI BsaXI BseYI BsiHKAI BsmI BsmBI Bsp1286I BsrFI BssSI BstXI BstYI BstZ17I Bsu36I BtgI BtsI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoO109I HaeII Hin4I KasI MslI MspA1I NarI NcoI NspI PflMI PfoI PpuMI PshAI PspOMI PstI PvuII RsrII SacI SfcI SfoI SmaI SpeI SphI StuI StyI TaqII TstI XbaI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AflII AflIII AgeI AhdI AloI ApoI AscI AseI AsiSI BaeI BamHI BbsI BciVI BclI BfrBI BglII BmgBI BmtI BplI BpuEI BsaBI BsaWI BseRI BsgI BsiEI BsiWI BspEI BspHI BspMI BsrBI BsrDI BsrGI BssHII BstAPI BstBI BstEII BtgZI ClaI CspCI DraI DrdI EagI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI MscI NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PmeI PmlI PpiI PsiI PspXI PsrI PvuI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmlI SnaBI SrfI SspI SwaI TatI Tth111I XhoI ZraI