################################## # NO CUT ENZYMES ( 174 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AvaI AviII BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BcnI BfrI BglI BglII BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssKI BssSI Bst1107I BstAPI BstBI BstEII BstNI BstPI BstUI BstXI BstYI BstZ17I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco52I EcoNI EcoO65I EcoRI EcoRII EcoRV EcoT22I FseI FspI HapII Hin1I HincII HindII HindIII HpaI HpaII HphI KasI KpnI KspI MaeII MaeIII MamI MfeI MflI MluI MroI MseI MspI MunI MvaI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SbfI ScaI ScrFI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TaqI Tru9I Tsp45I Tsp509I TthHB8I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AfaI 154 GTAC GT^AC AocI 46 CCTCAGG CC^T[ACGT]AGG Aor51HI 476 AGCGCT AGC^GCT AspHI 341 GAGCAC G[AT]GC[AT]^C AspI 85 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 570 CCTAGG C^CTAGG BalI 251 TGGCCA TGG^CCA BanII 496 GAGCCC G[GA]GC[CT]^C BbvI 448 GCAGC GCAGC(8/12) BclI 74 TGATCA T^GATCA BlnI 570 CCTAGG C^CTAGG BseRI 487 GAGGAG GAGGAG(10/8) BsiHKAI 341 GAGCAC G[AT]GC[AT]^C BsmFI 321 GGGAC GGGAC(10/14) BsrI 501 CCAGT ACTGG(1/-1) Bsu36I 46 CCTCAGG CC^T[ACGT]AGG DpnII 75 GATC ^GATC DraII 321 GGGACCC [GA]G^G[ACGT]CC[CT] EaeI 251 TGGCCA [CT]^GGCC[GA] EarI 493 GAAGAG CTCTTC(1/4) Eco47III 476 AGCGCT AGC^GCT Eco81I 46 CCTCAGG CC^T[ACGT]AGG EcoO109I 321 GGGACCC [GA]G^G[ACGT]CC[CT] FbaI 74 TGATCA T^GATCA Fnu4HI 448 GCAGC GC^[ACGT]GC HaeII 476 AGCGCT [GA]GCGC^[CT] ItaI 448 GCAGC GC^[ACGT]GC Ksp632I 493 GAAGAG CTCTTC(1/4) MboI 75 GATC ^GATC MluNI 251 TGGCCA TGG^CCA MscI 251 TGGCCA TGG^CCA MspA1I 255 CAGCGG C[AC]G^C[GT]G NcoI 408 CCATGG C^CATGG NdeII 75 GATC ^GATC PflFI 85 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 6 CCAGAATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 418 GACTC GAGTC(4/5) PpuMI 321 GGGACCC [GA]G^G[AT]CC[CT] RsaI 154 GTAC GT^AC SapI 493 GAAGAGC GCTCTTC(1/4) Sau3AI 75 GATC ^GATC SfaNI 292 GATGC GCATC(5/9) SfcI 525 CTATAG C^T[GA][CT]AG StuI 567 AGGCCT AGG^CCT TseI 448 GCAGC G^C[AT]GC Tth111I 85 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 6 CCAGAATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AciI 396 CCGC CCGC(-3/-1) 257 GCGG AlwNI 7 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG 232 CAGAGGCTG AvaII 322 GGACC G^G[AT]CC 574 GGACC BfaI 67 CTAG C^TAG 571 CTAG BmyI 341 GAGCAC G[AGT]GC[ACT]^C 496 GAGCCC Bsp1286I 341 GAGCAC G[AGT]GC[ACT]^C 496 GAGCCC CfoI 477 GCGC GCG^C 507 GCGC DdeI 47 CTCAG C^T[ACGT]AG 133 CTAAG DsaI 369 CCGTGG C^C[GA][CT]GG 408 CCATGG EcoT14I 408 CCATGG C^C[AT][AT]GG 570 CCTAGG FauI 395 CCCGC CCCGC(4/6) 257 GCGGG HgaI 85 GACGC GACGC(5/10) 312 GACGC HhaI 477 GCGC GCG^C 507 GCGC HinP1I 477 GCGC G^CGC 507 GCGC MaeI 67 CTAG C^TAG 571 CTAG MslI 286 CACAAGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 327 CACCAGAGTG StyI 408 CCATGG C^C[AT][AT]GG 570 CCTAGG XcmI 501 CCAGTTGCGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 546 CCACTATGTAGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 19 enzymes) # ######################################### AluI BpmI BsaJI BsiYI BslI BstF5I Cac8I Cfr13I FokI HaeIII HinfI MboII MnlI MwoI NlaIII NlaIV Sau96I TfiI TspRI