################################## # NO CUT ENZYMES ( 106 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AflIII AocI Aor51HI AscI AseI AsnI Asp718I AspI AvaI AviII AvrII BalI BamHI BbeI BbrPI BclI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaHI BseAI BsiWI BsmBI BsoBI BspEI BspHI BspLU11I BspMI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CelII CpoI DraI DraIII EarI Eco47III Eco81I EcoNI EcoO65I EcoRI EcoRV FbaI FseI FspI Hin1I KasI KpnI Ksp632I MluI MluNI MroI MscI NarI NcoI NdeI NheI NotI NruI NspV PacI PaeR7I PflFI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I StuI SwaI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AccI 493 GTAGAC GT^[AC][GT]AC AgeI 1141 ACCGGT A^CCGGT AhdI 342 GACAGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 617 GTGCAC G^TGCAC AlwNI 920 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 363 GGGCCC GGGCC^C ApaLI 617 GTGCAC G^TGCAC AspEI 342 GACAGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BanI 724 GGTGCC G^G[CT][GA]CC BanII 363 GGGCCC G[GA]GC[CT]^C BbsI 1501 GTCTTC GAAGAC(2/6) BbvCI 1303 GCTGAGG CCTCAGC(-5/-2) BcgI 56 CGAGCGGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 295 GTATCC GTATCC(6/5) BglII 656 AGATCT A^GATCT BpuAI 1501 GTCTTC GAAGAC(2/6) BsaBI 868 GATGGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 1141 ACCGGT [AT]^CCGG[AT] BsgI 1667 CTGCAC GTGCAG(16/14) BsmI 956 GCATTC GAATGC(1/-1) Bsp1407I 1547 TGTACA T^GTACA BspDI 1552 ATCGAT AT^CGAT BsrGI 1547 TGTACA T^GTACA ClaI 1552 ATCGAT AT^CGAT EagI 186 CGGCCG C^GGCCG Eam1105I 342 GACAGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 186 CGGCCG C^GGCCG Eco52I 186 CGGCCG C^GGCCG EcoT14I 498 CCTTGG C^C[AT][AT]GG EcoT22I 673 ATGCAT ATGCA^T HaeII 94 AGCGCC [GA]GCGC^[CT] HincII 745 GTTAAC GT[CT]^[GA]AC HindII 745 GTTAAC GT[CT]^[GA]AC HindIII 578 AAGCTT A^AGCTT HpaI 745 GTTAAC GTT^AAC KspI 984 CCGCGG CCGC^GG MaeII 1571 ACGT A^CGT MamI 868 GATGGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 458 CAATTG C^AATTG MunI 458 CAATTG C^AATTG NaeI 144 GCCGGC GCC^GGC NgoMIV 144 GCCGGC G^CCGGC NsiI 673 ATGCAT ATGCA^T NspI 1001 GCATGC [GA]CATG^[CT] PflMI 1435 CCAGTGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 1141 ACCGGT A^CCGGT PleI 922 GACTC GAGTC(4/5) PshAI 975 GACTGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 1645 CTGCAG CTGCA^G SacII 984 CCGCGG CCGC^GG SfcI 1645 CTGCAG C^T[GA][CT]AG SphI 1001 GCATGC GCATG^C SspBI 1547 TGTACA T^GTACA SspI 394 AATATT AAT^ATT StyI 498 CCTTGG C^C[AT][AT]GG Van91I 1435 CCAGTGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 164 CCACGCTGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AfaI 33 GTAC GT^AC 1548 GTAC AspHI 617 GTGCAC G[AT]GC[AT]^C 1016 GTGCTC BfaI 1034 CTAG C^TAG 1204 CTAG BpmI 416 CTCCAG CTGGAG(16/14) 760 CTGGAG BsaI 820 GAGACC GGTCTC(1/5) 1060 GAGACC BseRI 1126 GAGGAG GAGGAG(10/8) 1291 GAGGAG BsiEI 186 CGGCCG CG[GA][CT]^CG 249 CGGTCG BsiHKAI 617 GTGCAC G[AT]GC[AT]^C 1016 GTGCTC BsmAI 820 GAGAC GTCTC(1/5) 1060 GAGAC BsrBI 57 GAGCGG CCGCTC(-3/-3) 1512 GAGCGG BsrDI 936 CATTGC GCAATG(2/0) 1623 CATTGC BssSI 54 CACGAG CACGAG(-5/-1) 1008 CACGAG BstYI 656 AGATCT [GA]^GATC[CT] 704 AGATCC DrdI 822 GACCATGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1591 GACCCCCAGGTC DsaI 137 CCGTGG C^C[GA][CT]GG 984 CCGCGG EaeI 186 CGGCCG [CT]^GGCC[GA] 1260 TGGCCG HgaI 1099 GACGC GACGC(5/10) 1661 GACGC MaeI 1034 CTAG C^TAG 1204 CTAG MaeIII 13 GTGAC ^GT[ACGT]AC 350 GTCAC MflI 656 AGATCT [GA]^GATC[CT] 704 AGATCC MslI 165 CACGCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1153 CACATCCGTG PpuMI 408 AGGTCCT [GA]G^G[AT]CC[CT] 1589 AGGACCC PvuII 806 CAGCTG CAG^CTG 1642 CAGCTG RsaI 33 GTAC GT^AC 1548 GTAC SexAI 812 ACCTGGT A^CC[AT]GGT 1276 ACCAGGT Tsp45I 13 GTGAC ^GT[GC]AC 350 GTCAC XhoII 656 AGATCT [GA]^GATC[CT] 704 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 68 enzymes) # ######################################### AccII AciI AcsI AluI AlwI ApoI Asp700I AvaII BbvI BcnI BmyI BsaJI BsiYI BslI BsmFI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MboI MboII MnlI MseI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I XmnI